Raspberry bushy dwarf virus (isolate Malling Jewel raspberry/R15) (RBDV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Mayoviridae; Idaeovirus; Raspberry bushy dwarf virus

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q86564|CAPSD_RBDVR Capsid protein OS=Raspberry bushy dwarf virus (isolate Malling Jewel raspberry/R15) OX=652675 PE=4 SV=1
MM1 pKa = 7.62FSRR4 pKa = 11.84SSSTRR9 pKa = 11.84SSLVGSRR16 pKa = 11.84SGSIFGGGSVKK27 pKa = 10.39KK28 pKa = 10.33SSTVRR33 pKa = 11.84GFSAGLEE40 pKa = 4.08RR41 pKa = 11.84SRR43 pKa = 11.84GLPSASAGEE52 pKa = 4.19NQISLPGLRR61 pKa = 11.84IPVKK65 pKa = 10.64ASSQPGNYY73 pKa = 8.74YY74 pKa = 10.51LKK76 pKa = 10.55EE77 pKa = 4.12RR78 pKa = 11.84GIDD81 pKa = 3.63LPIVQQQKK89 pKa = 10.17FLAADD94 pKa = 3.7GKK96 pKa = 11.42EE97 pKa = 3.81MGEE100 pKa = 4.58CYY102 pKa = 10.83LLDD105 pKa = 3.61TSRR108 pKa = 11.84TDD110 pKa = 4.17LLDD113 pKa = 3.75AAKK116 pKa = 10.43AALNEE121 pKa = 4.4SNLLEE126 pKa = 4.07FNKK129 pKa = 10.11FKK131 pKa = 10.77EE132 pKa = 4.18FKK134 pKa = 10.22KK135 pKa = 11.08YY136 pKa = 9.93KK137 pKa = 9.95GKK139 pKa = 10.27NNEE142 pKa = 3.71FSLVEE147 pKa = 3.65ASVFDD152 pKa = 3.75KK153 pKa = 11.17LIRR156 pKa = 11.84KK157 pKa = 9.29DD158 pKa = 3.8DD159 pKa = 4.0SPIHH163 pKa = 6.27LNRR166 pKa = 11.84LLIAVLPAVGKK177 pKa = 7.54GTPGTARR184 pKa = 11.84IKK186 pKa = 10.63IRR188 pKa = 11.84DD189 pKa = 3.44ARR191 pKa = 11.84LDD193 pKa = 3.69DD194 pKa = 4.82GYY196 pKa = 11.89GEE198 pKa = 4.73LFSSEE203 pKa = 3.89NRR205 pKa = 11.84VDD207 pKa = 3.62SGYY210 pKa = 10.25IYY212 pKa = 9.88CINVGYY218 pKa = 9.87SVPKK222 pKa = 10.58SEE224 pKa = 4.7IDD226 pKa = 3.37YY227 pKa = 10.67KK228 pKa = 11.19INIDD232 pKa = 3.69FAGVPIKK239 pKa = 10.61DD240 pKa = 3.58GKK242 pKa = 10.25SPIWVKK248 pKa = 10.66AAFSLAGGPPVFLDD262 pKa = 3.54GTMSLGAEE270 pKa = 4.28ILPDD274 pKa = 3.39SHH276 pKa = 8.24KK277 pKa = 10.95EE278 pKa = 3.84LLGTSALLLNEE289 pKa = 4.37ANSNRR294 pKa = 11.84KK295 pKa = 9.19SFSGDD300 pKa = 3.2DD301 pKa = 3.49GEE303 pKa = 5.03LRR305 pKa = 11.84RR306 pKa = 11.84DD307 pKa = 3.5YY308 pKa = 10.42PYY310 pKa = 11.31KK311 pKa = 10.25RR312 pKa = 11.84FEE314 pKa = 4.79EE315 pKa = 4.26ISPLDD320 pKa = 4.19SISQVDD326 pKa = 3.81TASQDD331 pKa = 3.4SVNEE335 pKa = 3.97VNTEE339 pKa = 3.72NVQNGTGEE347 pKa = 4.46VYY349 pKa = 10.73LAPPSHH355 pKa = 6.52SVYY358 pKa = 11.18

Molecular weight: 38.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q86564|CAPSD_RBDVR Capsid protein OS=Raspberry bushy dwarf virus (isolate Malling Jewel raspberry/R15) OX=652675 PE=4 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.45KK4 pKa = 10.15AVPPIVKK11 pKa = 9.74AQYY14 pKa = 9.74EE15 pKa = 4.47LYY17 pKa = 10.33NRR19 pKa = 11.84KK20 pKa = 9.49LNRR23 pKa = 11.84AIKK26 pKa = 10.6VSGSQKK32 pKa = 10.72KK33 pKa = 10.18LDD35 pKa = 3.55ASFVGFSEE43 pKa = 4.29SSNPEE48 pKa = 3.6TGKK51 pKa = 9.35PHH53 pKa = 7.55ADD55 pKa = 2.83MSMSAKK61 pKa = 9.62VKK63 pKa = 10.4RR64 pKa = 11.84VNTWLKK70 pKa = 10.92NFDD73 pKa = 3.34RR74 pKa = 11.84EE75 pKa = 4.07YY76 pKa = 10.32WDD78 pKa = 3.32NQFASKK84 pKa = 10.1PIPRR88 pKa = 11.84PAKK91 pKa = 9.57QVLKK95 pKa = 10.49GSSSKK100 pKa = 10.55SQQRR104 pKa = 11.84DD105 pKa = 2.99EE106 pKa = 4.84GEE108 pKa = 4.29VVFTRR113 pKa = 11.84KK114 pKa = 9.98DD115 pKa = 3.33SQKK118 pKa = 10.42SVRR121 pKa = 11.84TVSYY125 pKa = 8.48WVCTPEE131 pKa = 5.89KK132 pKa = 10.67SMKK135 pKa = 8.3PLKK138 pKa = 10.58YY139 pKa = 10.24KK140 pKa = 10.2EE141 pKa = 4.42DD142 pKa = 3.79EE143 pKa = 4.33NVVEE147 pKa = 4.45VTFNDD152 pKa = 3.91LAAQKK157 pKa = 10.75AGDD160 pKa = 3.89KK161 pKa = 10.47LVSILLEE168 pKa = 4.07INVVGGAVDD177 pKa = 3.46DD178 pKa = 4.31KK179 pKa = 11.67GRR181 pKa = 11.84VAVLEE186 pKa = 3.9KK187 pKa = 10.72DD188 pKa = 3.18AAVTVDD194 pKa = 3.79YY195 pKa = 11.19LLGSPYY201 pKa = 10.51EE202 pKa = 4.47AINLVSGLNKK212 pKa = 9.97INFRR216 pKa = 11.84SMTDD220 pKa = 3.24VVDD223 pKa = 5.42SIPSLLNEE231 pKa = 3.95RR232 pKa = 11.84KK233 pKa = 9.88VCVFQNDD240 pKa = 3.94DD241 pKa = 3.37SSSFYY246 pKa = 9.67IRR248 pKa = 11.84KK249 pKa = 7.9WANFLQEE256 pKa = 3.96VSAVLPVGTGKK267 pKa = 10.68SSTIVLTT274 pKa = 3.95

Molecular weight: 30.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2326

274

1694

775.3

86.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.89 ± 0.359

1.978 ± 0.639

6.793 ± 0.224

5.374 ± 0.244

4.772 ± 0.4

6.105 ± 0.835

2.107 ± 0.708

5.546 ± 0.484

6.922 ± 0.954

9.114 ± 0.418

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.677 ± 0.236

4.729 ± 0.246

4.385 ± 0.192

2.537 ± 0.259

5.245 ± 0.216

9.329 ± 1.139

5.116 ± 0.783

7.696 ± 0.921

1.075 ± 0.213

3.611 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski