Amino acid dipepetide frequency for Megaselia scalaris (Humpbacked fly) (Phora scalaris)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.248AlaAla: 4.248 ± 0.072	0.986AlaCys: 0.986 ± 0.025	2.453AlaAsp: 2.453 ± 0.031	3.182AlaGlu: 3.182 ± 0.04	2.261AlaPhe: 2.261 ± 0.03	2.928AlaGly: 2.928 ± 0.043	1.165AlaHis: 1.165 ± 0.022	3.473AlaIle: 3.473 ± 0.042	3.539AlaLys: 3.539 ± 0.046	4.863AlaLeu: 4.863 ± 0.052	1.374AlaMet: 1.374 ± 0.024	2.692AlaAsn: 2.692 ± 0.034	2.573AlaPro: 2.573 ± 0.049	2.024AlaGln: 2.024 ± 0.031	2.251AlaArg: 2.251 ± 0.03	3.966AlaSer: 3.966 ± 0.041	3.11AlaThr: 3.11 ± 0.044	3.293AlaVal: 3.293 ± 0.037	0.48AlaTrp: 0.48 ± 0.014	1.593AlaTyr: 1.593 ± 0.024	0.016AlaXaa: 0.016 ± 0.003
Cys	0.93CysAla: 0.93 ± 0.021	0.46CysCys: 0.46 ± 0.015	1.109CysAsp: 1.109 ± 0.025	1.18CysGlu: 1.18 ± 0.029	0.883CysPhe: 0.883 ± 0.019	1.346CysGly: 1.346 ± 0.038	0.463CysHis: 0.463 ± 0.015	1.26CysIle: 1.26 ± 0.032	1.276CysLys: 1.276 ± 0.029	1.816CysLeu: 1.816 ± 0.038	0.437CysMet: 0.437 ± 0.014	1.045CysAsn: 1.045 ± 0.024	1.03CysPro: 1.03 ± 0.028	0.736CysGln: 0.736 ± 0.021	0.93CysArg: 0.93 ± 0.025	1.668CysSer: 1.668 ± 0.039	1.041CysThr: 1.041 ± 0.024	1.147CysVal: 1.147 ± 0.033	0.223CysTrp: 0.223 ± 0.009	0.624CysTyr: 0.624 ± 0.016	0.008CysXaa: 0.008 ± 0.002
Asp	2.327AspAla: 2.327 ± 0.033	1.09AspCys: 1.09 ± 0.028	3.539AspAsp: 3.539 ± 0.055	4.084AspGlu: 4.084 ± 0.054	2.692AspPhe: 2.692 ± 0.036	2.872AspGly: 2.872 ± 0.04	1.046AspHis: 1.046 ± 0.019	3.803AspIle: 3.803 ± 0.045	3.284AspLys: 3.284 ± 0.04	4.82AspLeu: 4.82 ± 0.054	1.243AspMet: 1.243 ± 0.022	2.738AspAsn: 2.738 ± 0.038	2.218AspPro: 2.218 ± 0.042	1.609AspGln: 1.609 ± 0.027	2.129AspArg: 2.129 ± 0.035	3.981AspSer: 3.981 ± 0.047	2.349AspThr: 2.349 ± 0.03	3.093AspVal: 3.093 ± 0.036	0.597AspTrp: 0.597 ± 0.016	1.844AspTyr: 1.844 ± 0.029	0.014AspXaa: 0.014 ± 0.002
Glu	3.292GluAla: 3.292 ± 0.04	1.24GluCys: 1.24 ± 0.033	4.027GluAsp: 4.027 ± 0.042	5.776GluGlu: 5.776 ± 0.103	2.533GluPhe: 2.533 ± 0.039	2.811GluGly: 2.811 ± 0.036	1.33GluHis: 1.33 ± 0.025	4.873GluIle: 4.873 ± 0.054	5.537GluLys: 5.537 ± 0.077	5.324GluLeu: 5.324 ± 0.051	1.655GluMet: 1.655 ± 0.029	4.442GluAsn: 4.442 ± 0.044	2.239GluPro: 2.239 ± 0.044	2.347GluGln: 2.347 ± 0.04	2.919GluArg: 2.919 ± 0.039	4.491GluSer: 4.491 ± 0.063	3.316GluThr: 3.316 ± 0.042	3.588GluVal: 3.588 ± 0.045	0.617GluTrp: 0.617 ± 0.018	2.044GluTyr: 2.044 ± 0.033	0.026GluXaa: 0.026 ± 0.003
Phe	2.236PheAla: 2.236 ± 0.028	0.976PheCys: 0.976 ± 0.019	2.36PheAsp: 2.36 ± 0.033	2.735PheGlu: 2.735 ± 0.036	2.004PhePhe: 2.004 ± 0.032	2.718PheGly: 2.718 ± 0.046	1.073PheHis: 1.073 ± 0.019	2.751PheIle: 2.751 ± 0.037	2.89PheLys: 2.89 ± 0.038	4.274PheLeu: 4.274 ± 0.046	1.079PheMet: 1.079 ± 0.021	2.337PheAsn: 2.337 ± 0.032	1.892PhePro: 1.892 ± 0.031	1.758PheGln: 1.758 ± 0.028	1.874PheArg: 1.874 ± 0.028	3.403PheSer: 3.403 ± 0.042	2.246PheThr: 2.246 ± 0.033	2.636PheVal: 2.636 ± 0.034	0.508PheTrp: 0.508 ± 0.015	1.65PheTyr: 1.65 ± 0.028	0.015PheXaa: 0.015 ± 0.002
Gly	2.873GlyAla: 2.873 ± 0.041	0.988GlyCys: 0.988 ± 0.025	2.748GlyAsp: 2.748 ± 0.041	2.969GlyGlu: 2.969 ± 0.053	2.556GlyPhe: 2.556 ± 0.032	4.194GlyGly: 4.194 ± 0.086	1.393GlyHis: 1.393 ± 0.033	3.505GlyIle: 3.505 ± 0.048	3.559GlyLys: 3.559 ± 0.041	4.33GlyLeu: 4.33 ± 0.05	1.205GlyMet: 1.205 ± 0.028	3.094GlyAsn: 3.094 ± 0.039	2.134GlyPro: 2.134 ± 0.049	1.961GlyGln: 1.961 ± 0.036	2.45GlyArg: 2.45 ± 0.037	4.414GlySer: 4.414 ± 0.059	2.912GlyThr: 2.912 ± 0.038	3.2GlyVal: 3.2 ± 0.042	0.635GlyTrp: 0.635 ± 0.018	2.012GlyTyr: 2.012 ± 0.038	0.031GlyXaa: 0.031 ± 0.004
His	0.967HisAla: 0.967 ± 0.021	0.557HisCys: 0.557 ± 0.012	0.95HisAsp: 0.95 ± 0.019	1.279HisGlu: 1.279 ± 0.024	1.15HisPhe: 1.15 ± 0.019	1.286HisGly: 1.286 ± 0.032	1.115HisHis: 1.115 ± 0.037	1.539HisIle: 1.539 ± 0.024	1.448HisLys: 1.448 ± 0.024	2.263HisLeu: 2.263 ± 0.029	0.614HisMet: 0.614 ± 0.018	1.233HisAsn: 1.233 ± 0.026	1.234HisPro: 1.234 ± 0.024	1.166HisGln: 1.166 ± 0.024	1.168HisArg: 1.168 ± 0.019	1.879HisSer: 1.879 ± 0.03	1.151HisThr: 1.151 ± 0.023	1.202HisVal: 1.202 ± 0.024	0.279HisTrp: 0.279 ± 0.009	0.844HisTyr: 0.844 ± 0.02	0.007HisXaa: 0.007 ± 0.002
Ile	3.563IleAla: 3.563 ± 0.037	1.516IleCys: 1.516 ± 0.035	3.466IleAsp: 3.466 ± 0.038	4.118IleGlu: 4.118 ± 0.045	3.092IlePhe: 3.092 ± 0.04	3.289IleGly: 3.289 ± 0.037	1.524IleHis: 1.524 ± 0.025	4.192IleIle: 4.192 ± 0.048	4.314IleLys: 4.314 ± 0.043	6.345IleLeu: 6.345 ± 0.053	1.496IleMet: 1.496 ± 0.026	3.492IleAsn: 3.492 ± 0.048	3.413IlePro: 3.413 ± 0.041	2.661IleGln: 2.661 ± 0.037	2.879IleArg: 2.879 ± 0.035	5.485IleSer: 5.485 ± 0.049	3.53IleThr: 3.53 ± 0.043	3.883IleVal: 3.883 ± 0.046	0.705IleTrp: 0.705 ± 0.018	2.131IleTyr: 2.131 ± 0.026	0.019IleXaa: 0.019 ± 0.003
Lys	3.483LysAla: 3.483 ± 0.043	1.501LysCys: 1.501 ± 0.039	3.875LysAsp: 3.875 ± 0.049	5.094LysGlu: 5.094 ± 0.072	2.881LysPhe: 2.881 ± 0.039	3.056LysGly: 3.056 ± 0.05	1.62LysHis: 1.62 ± 0.028	4.771LysIle: 4.771 ± 0.045	5.902LysLys: 5.902 ± 0.077	6.158LysLeu: 6.158 ± 0.06	1.783LysMet: 1.783 ± 0.027	4.197LysAsn: 4.197 ± 0.042	3.063LysPro: 3.063 ± 0.047	2.693LysGln: 2.693 ± 0.034	3.513LysArg: 3.513 ± 0.049	5.421LysSer: 5.421 ± 0.063	4.043LysThr: 4.043 ± 0.044	3.912LysVal: 3.912 ± 0.039	0.746LysTrp: 0.746 ± 0.018	2.571LysTyr: 2.571 ± 0.035	0.033LysXaa: 0.033 ± 0.004
Leu	4.941LeuAla: 4.941 ± 0.055	1.662LeuCys: 1.662 ± 0.028	4.509LeuAsp: 4.509 ± 0.047	5.931LeuGlu: 5.931 ± 0.064	3.632LeuPhe: 3.632 ± 0.044	4.3LeuGly: 4.3 ± 0.046	2.057LeuHis: 2.057 ± 0.032	5.338LeuIle: 5.338 ± 0.056	6.852LeuLys: 6.852 ± 0.061	7.806LeuLeu: 7.806 ± 0.077	2.178LeuMet: 2.178 ± 0.03	4.931LeuAsn: 4.931 ± 0.044	4.25LeuPro: 4.25 ± 0.042	4.025LeuGln: 4.025 ± 0.047	4.376LeuArg: 4.376 ± 0.042	6.823LeuSer: 6.823 ± 0.06	4.756LeuThr: 4.756 ± 0.045	4.834LeuVal: 4.834 ± 0.048	0.876LeuTrp: 0.876 ± 0.018	2.765LeuTyr: 2.765 ± 0.035	0.033LeuXaa: 0.033 ± 0.004
Met	1.432MetAla: 1.432 ± 0.023	0.412MetCys: 0.412 ± 0.014	1.394MetAsp: 1.394 ± 0.026	1.714MetGlu: 1.714 ± 0.028	1.103MetPhe: 1.103 ± 0.021	1.303MetGly: 1.303 ± 0.025	0.493MetHis: 0.493 ± 0.014	1.389MetIle: 1.389 ± 0.03	1.938MetLys: 1.938 ± 0.029	1.996MetLeu: 1.996 ± 0.029	0.702MetMet: 0.702 ± 0.019	1.403MetAsn: 1.403 ± 0.026	1.049MetPro: 1.049 ± 0.023	0.91MetGln: 0.91 ± 0.022	1.07MetArg: 1.07 ± 0.022	1.896MetSer: 1.896 ± 0.025	1.293MetThr: 1.293 ± 0.023	1.323MetVal: 1.323 ± 0.023	0.242MetTrp: 0.242 ± 0.009	0.796MetTyr: 0.796 ± 0.018	0.012MetXaa: 0.012 ± 0.002
Asn	2.647AsnAla: 2.647 ± 0.035	1.209AsnCys: 1.209 ± 0.024	2.831AsnAsp: 2.831 ± 0.032	3.609AsnGlu: 3.609 ± 0.04	2.72AsnPhe: 2.72 ± 0.033	3.426AsnGly: 3.426 ± 0.051	1.332AsnHis: 1.332 ± 0.03	4.196AsnIle: 4.196 ± 0.044	3.569AsnLys: 3.569 ± 0.038	5.279AsnLeu: 5.279 ± 0.054	1.384AsnMet: 1.384 ± 0.026	4.056AsnAsn: 4.056 ± 0.067	2.593AsnPro: 2.593 ± 0.038	2.225AsnGln: 2.225 ± 0.037	2.453AsnArg: 2.453 ± 0.033	5.016AsnSer: 5.016 ± 0.06	2.95AsnThr: 2.95 ± 0.036	3.29AsnVal: 3.29 ± 0.038	0.607AsnTrp: 0.607 ± 0.015	2.063AsnTyr: 2.063 ± 0.028	0.019AsnXaa: 0.019 ± 0.003
Pro	2.462ProAla: 2.462 ± 0.045	0.736ProCys: 0.736 ± 0.044	2.151ProAsp: 2.151 ± 0.029	3.169ProGlu: 3.169 ± 0.043	1.854ProPhe: 1.854 ± 0.031	2.53ProGly: 2.53 ± 0.07	1.085ProHis: 1.085 ± 0.023	3.008ProIle: 3.008 ± 0.034	3.352ProLys: 3.352 ± 0.049	3.653ProLeu: 3.653 ± 0.039	1.019ProMet: 1.019 ± 0.02	2.655ProAsn: 2.655 ± 0.04	3.588ProPro: 3.588 ± 0.071	2.297ProGln: 2.297 ± 0.05	1.944ProArg: 1.944 ± 0.033	4.016ProSer: 4.016 ± 0.048	2.948ProThr: 2.948 ± 0.043	2.772ProVal: 2.772 ± 0.042	0.397ProTrp: 0.397 ± 0.012	1.538ProTyr: 1.538 ± 0.031	0.017ProXaa: 0.017 ± 0.003
Gln	1.988GlnAla: 1.988 ± 0.033	0.74GlnCys: 0.74 ± 0.021	1.648GlnAsp: 1.648 ± 0.026	2.539GlnGlu: 2.539 ± 0.038	1.575GlnPhe: 1.575 ± 0.026	1.803GlnGly: 1.803 ± 0.042	1.172GlnHis: 1.172 ± 0.027	2.77GlnIle: 2.77 ± 0.036	3.0GlnLys: 3.0 ± 0.039	3.393GlnLeu: 3.393 ± 0.043	1.093GlnMet: 1.093 ± 0.019	2.618GlnAsn: 2.618 ± 0.035	1.927GlnPro: 1.927 ± 0.04	3.368GlnGln: 3.368 ± 0.116	2.05GlnArg: 2.05 ± 0.033	3.001GlnSer: 3.001 ± 0.041	2.288GlnThr: 2.288 ± 0.032	2.019GlnVal: 2.019 ± 0.034	0.396GlnTrp: 0.396 ± 0.014	1.321GlnTyr: 1.321 ± 0.025	0.019GlnXaa: 0.019 ± 0.003
Arg	2.187ArgAla: 2.187 ± 0.032	0.833ArgCys: 0.833 ± 0.022	2.24ArgAsp: 2.24 ± 0.034	2.693ArgGlu: 2.693 ± 0.037	1.925ArgPhe: 1.925 ± 0.031	2.257ArgGly: 2.257 ± 0.036	1.153ArgHis: 1.153 ± 0.025	3.036ArgIle: 3.036 ± 0.034	3.799ArgLys: 3.799 ± 0.041	3.823ArgLeu: 3.823 ± 0.044	1.148ArgMet: 1.148 ± 0.019	2.924ArgAsn: 2.924 ± 0.037	2.026ArgPro: 2.026 ± 0.031	1.919ArgGln: 1.919 ± 0.031	2.89ArgArg: 2.89 ± 0.043	3.451ArgSer: 3.451 ± 0.049	2.398ArgThr: 2.398 ± 0.031	2.266ArgVal: 2.266 ± 0.03	0.466ArgTrp: 0.466 ± 0.015	1.455ArgTyr: 1.455 ± 0.021	0.019ArgXaa: 0.019 ± 0.003
Ser	4.21SerAla: 4.21 ± 0.051	1.475SerCys: 1.475 ± 0.033	3.958SerAsp: 3.958 ± 0.04	4.688SerGlu: 4.688 ± 0.056	3.367SerPhe: 3.367 ± 0.037	4.67SerGly: 4.67 ± 0.054	1.744SerHis: 1.744 ± 0.034	4.876SerIle: 4.876 ± 0.054	5.467SerLys: 5.467 ± 0.052	6.886SerLeu: 6.886 ± 0.06	1.815SerMet: 1.815 ± 0.026	4.822SerAsn: 4.822 ± 0.052	4.106SerPro: 4.106 ± 0.066	3.093SerGln: 3.093 ± 0.04	3.472SerArg: 3.472 ± 0.046	9.273SerSer: 9.273 ± 0.124	5.192SerThr: 5.192 ± 0.058	4.363SerVal: 4.363 ± 0.047	0.844SerTrp: 0.844 ± 0.02	2.445SerTyr: 2.445 ± 0.034	0.029SerXaa: 0.029 ± 0.004
Thr	3.158ThrAla: 3.158 ± 0.041	1.095ThrCys: 1.095 ± 0.027	2.633ThrAsp: 2.633 ± 0.039	3.298ThrGlu: 3.298 ± 0.043	2.373ThrPhe: 2.373 ± 0.035	2.908ThrGly: 2.908 ± 0.039	1.157ThrHis: 1.157 ± 0.023	3.815ThrIle: 3.815 ± 0.039	3.681ThrLys: 3.681 ± 0.043	4.712ThrLeu: 4.712 ± 0.046	1.252ThrMet: 1.252 ± 0.021	3.197ThrAsn: 3.197 ± 0.037	3.186ThrPro: 3.186 ± 0.045	1.984ThrGln: 1.984 ± 0.03	2.097ThrArg: 2.097 ± 0.029	4.972ThrSer: 4.972 ± 0.057	4.406ThrThr: 4.406 ± 0.081	3.277ThrVal: 3.277 ± 0.038	0.541ThrTrp: 0.541 ± 0.017	1.74ThrTyr: 1.74 ± 0.03	0.021ThrXaa: 0.021 ± 0.003
Val	3.401ValAla: 3.401 ± 0.039	1.212ValCys: 1.212 ± 0.029	3.063ValAsp: 3.063 ± 0.037	3.694ValGlu: 3.694 ± 0.047	2.63ValPhe: 2.63 ± 0.039	2.937ValGly: 2.937 ± 0.037	1.334ValHis: 1.334 ± 0.024	3.597ValIle: 3.597 ± 0.044	3.786ValLys: 3.786 ± 0.042	5.191ValLeu: 5.191 ± 0.053	1.307ValMet: 1.307 ± 0.022	2.953ValAsn: 2.953 ± 0.044	2.803ValPro: 2.803 ± 0.037	2.268ValGln: 2.268 ± 0.037	2.374ValArg: 2.374 ± 0.037	4.254ValSer: 4.254 ± 0.046	3.251ValThr: 3.251 ± 0.038	3.789ValVal: 3.789 ± 0.053	0.576ValTrp: 0.576 ± 0.016	1.868ValTyr: 1.868 ± 0.029	0.018ValXaa: 0.018 ± 0.003
Trp	0.494TrpAla: 0.494 ± 0.015	0.195TrpCys: 0.195 ± 0.01	0.529TrpAsp: 0.529 ± 0.016	0.618TrpGlu: 0.618 ± 0.017	0.49TrpPhe: 0.49 ± 0.014	0.589TrpGly: 0.589 ± 0.016	0.203TrpHis: 0.203 ± 0.009	0.759TrpIle: 0.759 ± 0.019	0.851TrpLys: 0.851 ± 0.02	0.919TrpLeu: 0.919 ± 0.02	0.293TrpMet: 0.293 ± 0.012	0.661TrpAsn: 0.661 ± 0.016	0.363TrpPro: 0.363 ± 0.014	0.345TrpGln: 0.345 ± 0.014	0.538TrpArg: 0.538 ± 0.015	0.786TrpSer: 0.786 ± 0.017	0.598TrpThr: 0.598 ± 0.018	0.548TrpVal: 0.548 ± 0.015	0.146TrpTrp: 0.146 ± 0.009	0.321TrpTyr: 0.321 ± 0.012	0.002TrpXaa: 0.002 ± 0.001
Tyr	1.611TyrAla: 1.611 ± 0.024	0.721TyrCys: 0.721 ± 0.017	1.868TyrAsp: 1.868 ± 0.026	2.039TyrGlu: 2.039 ± 0.029	1.676TyrPhe: 1.676 ± 0.031	1.914TyrGly: 1.914 ± 0.034	0.866TyrHis: 0.866 ± 0.021	2.139TyrIle: 2.139 ± 0.033	2.246TyrLys: 2.246 ± 0.032	2.993TyrLeu: 2.993 ± 0.037	0.771TyrMet: 0.771 ± 0.015	1.961TyrAsn: 1.961 ± 0.03	1.484TyrPro: 1.484 ± 0.028	1.326TyrGln: 1.326 ± 0.027	1.517TyrArg: 1.517 ± 0.021	2.578TyrSer: 2.578 ± 0.036	1.711TyrThr: 1.711 ± 0.026	1.851TyrVal: 1.851 ± 0.033	0.361TyrTrp: 0.361 ± 0.012	1.308TyrTyr: 1.308 ± 0.029	0.01TyrXaa: 0.01 ± 0.002
Xaa	0.019XaaAla: 0.019 ± 0.003	0.008XaaCys: 0.008 ± 0.002	0.019XaaAsp: 0.019 ± 0.003	0.023XaaGlu: 0.023 ± 0.003	0.019XaaPhe: 0.019 ± 0.003	0.025XaaGly: 0.025 ± 0.003	0.008XaaHis: 0.008 ± 0.002	0.024XaaIle: 0.024 ± 0.003	0.022XaaLys: 0.022 ± 0.003	0.03XaaLeu: 0.03 ± 0.003	0.02XaaMet: 0.02 ± 0.003	0.025XaaAsn: 0.025 ± 0.003	0.02XaaPro: 0.02 ± 0.003	0.014XaaGln: 0.014 ± 0.002	0.011XaaArg: 0.011 ± 0.002	0.022XaaSer: 0.022 ± 0.003	0.014XaaThr: 0.014 ± 0.002	0.021XaaVal: 0.021 ± 0.003	0.006XaaTrp: 0.006 ± 0.002	0.017XaaTyr: 0.017 ± 0.003	19.57XaaXaa: 19.57 ± 1.114

Statistics based on 11466 proteins (2583012 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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