Corynebacterium sp. KPL1989
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2066 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U7MJG2|U7MJG2_9CORY SWIM-type domain-containing protein OS=Corynebacterium sp. KPL1989 OX=1203622 GN=HMPREF1290_01959 PE=4 SV=1
MM1 pKa = 7.94 RR2 pKa = 11.84 YY3 pKa = 9.75 SSRR6 pKa = 11.84 FPLSFGQGKK15 pKa = 9.91 AFLALSLMAFLVACSSEE32 pKa = 4.13 IEE34 pKa = 4.22 QALPAASEE42 pKa = 4.19 SGGPVTISVRR52 pKa = 11.84 DD53 pKa = 3.57 AYY55 pKa = 9.47 GQHH58 pKa = 4.7 WVEE61 pKa = 5.46 FGVQCPYY68 pKa = 10.87 TSVDD72 pKa = 4.7 DD73 pKa = 4.16 ISEE76 pKa = 4.22 HH77 pKa = 6.93 LGLEE81 pKa = 3.86 PDD83 pKa = 3.25 EE84 pKa = 6.05 AYY86 pKa = 10.69 DD87 pKa = 3.78 LGSYY91 pKa = 7.62 DD92 pKa = 3.66 TQEE95 pKa = 3.78 VVYY98 pKa = 10.7 LKK100 pKa = 10.9 DD101 pKa = 3.17 ADD103 pKa = 3.92 GDD105 pKa = 3.78 VDD107 pKa = 5.51 FDD109 pKa = 3.78 VLDD112 pKa = 4.19 RR113 pKa = 11.84 LDD115 pKa = 3.44 VTLCSHH121 pKa = 7.68 RR122 pKa = 11.84 APEE125 pKa = 4.13 YY126 pKa = 9.48 FGYY129 pKa = 10.39 RR130 pKa = 11.84 GWLPADD136 pKa = 3.61 LPLIFEE142 pKa = 4.37 VNYY145 pKa = 10.67 DD146 pKa = 3.07 GWALTGVGDD155 pKa = 3.96 TTTGG159 pKa = 3.11
Molecular weight: 17.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.81
IPC2_protein 3.948
IPC_protein 3.935
Toseland 3.719
ProMoST 4.088
Dawson 3.923
Bjellqvist 4.075
Wikipedia 3.859
Rodwell 3.757
Grimsley 3.63
Solomon 3.91
Lehninger 3.872
Nozaki 4.037
DTASelect 4.266
Thurlkill 3.77
EMBOSS 3.859
Sillero 4.05
Patrickios 0.604
IPC_peptide 3.91
IPC2_peptide 4.024
IPC2.peptide.svr19 3.944
Protein with the highest isoelectric point:
>tr|U7MVC1|U7MVC1_9CORY DUF2183 domain-containing protein OS=Corynebacterium sp. KPL1989 OX=1203622 GN=HMPREF1290_00244 PE=4 SV=1
MM1 pKa = 7.69 AKK3 pKa = 10.06 GKK5 pKa = 8.69 RR6 pKa = 11.84 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 ARR17 pKa = 11.84 VHH19 pKa = 5.99 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVAARR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 9.67 KK40 pKa = 10.59 GRR42 pKa = 11.84 SKK44 pKa = 10.17 LTAA47 pKa = 4.04
Molecular weight: 5.48 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2066
0
2066
683554
27
3635
330.9
35.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.58 ± 0.068
0.712 ± 0.015
5.974 ± 0.049
6.318 ± 0.061
3.294 ± 0.034
8.242 ± 0.053
2.317 ± 0.025
5.13 ± 0.037
3.439 ± 0.053
9.371 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.16 ± 0.022
3.215 ± 0.036
4.885 ± 0.036
3.841 ± 0.032
5.987 ± 0.049
6.237 ± 0.04
5.904 ± 0.038
7.808 ± 0.043
1.339 ± 0.024
2.247 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here