Amino acid dipepetide frequency for Gracilibacillus boraciitolerans JCM 21714

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.62AlaAla: 4.62 ± 0.092	0.572AlaCys: 0.572 ± 0.028	3.418AlaAsp: 3.418 ± 0.056	4.324AlaGlu: 4.324 ± 0.071	2.986AlaPhe: 2.986 ± 0.062	4.467AlaGly: 4.467 ± 0.075	1.17AlaHis: 1.17 ± 0.033	6.205AlaIle: 6.205 ± 0.082	4.379AlaLys: 4.379 ± 0.073	6.291AlaLeu: 6.291 ± 0.091	1.821AlaMet: 1.821 ± 0.044	3.053AlaAsn: 3.053 ± 0.059	1.749AlaPro: 1.749 ± 0.042	2.118AlaGln: 2.118 ± 0.047	2.262AlaArg: 2.262 ± 0.055	3.836AlaSer: 3.836 ± 0.063	3.539AlaThr: 3.539 ± 0.066	4.496AlaVal: 4.496 ± 0.075	0.602AlaTrp: 0.602 ± 0.024	2.258AlaTyr: 2.258 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.335CysAla: 0.335 ± 0.018	0.108CysCys: 0.108 ± 0.01	0.359CysAsp: 0.359 ± 0.02	0.379CysGlu: 0.379 ± 0.02	0.322CysPhe: 0.322 ± 0.019	0.541CysGly: 0.541 ± 0.023	0.205CysHis: 0.205 ± 0.013	0.514CysIle: 0.514 ± 0.021	0.327CysLys: 0.327 ± 0.02	0.645CysLeu: 0.645 ± 0.026	0.193CysMet: 0.193 ± 0.014	0.321CysAsn: 0.321 ± 0.016	0.289CysPro: 0.289 ± 0.019	0.269CysGln: 0.269 ± 0.017	0.29CysArg: 0.29 ± 0.017	0.407CysSer: 0.407 ± 0.022	0.364CysThr: 0.364 ± 0.02	0.323CysVal: 0.323 ± 0.016	0.083CysTrp: 0.083 ± 0.009	0.279CysTyr: 0.279 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.246AspAla: 3.246 ± 0.058	0.327AspCys: 0.327 ± 0.016	2.862AspAsp: 2.862 ± 0.066	4.212AspGlu: 4.212 ± 0.064	2.528AspPhe: 2.528 ± 0.051	3.386AspGly: 3.386 ± 0.072	1.377AspHis: 1.377 ± 0.041	4.863AspIle: 4.863 ± 0.062	3.647AspLys: 3.647 ± 0.068	5.182AspLeu: 5.182 ± 0.071	1.473AspMet: 1.473 ± 0.039	2.457AspAsn: 2.457 ± 0.053	2.004AspPro: 2.004 ± 0.043	2.8AspGln: 2.8 ± 0.05	2.336AspArg: 2.336 ± 0.054	2.781AspSer: 2.781 ± 0.054	2.7AspThr: 2.7 ± 0.057	3.599AspVal: 3.599 ± 0.064	0.705AspTrp: 0.705 ± 0.024	2.426AspTyr: 2.426 ± 0.048	0.0AspXaa: 0.0 ± 0.0
Glu	5.12GluAla: 5.12 ± 0.085	0.303GluCys: 0.303 ± 0.019	4.147GluAsp: 4.147 ± 0.069	7.395GluGlu: 7.395 ± 0.12	2.494GluPhe: 2.494 ± 0.047	3.901GluGly: 3.901 ± 0.06	1.502GluHis: 1.502 ± 0.041	6.085GluIle: 6.085 ± 0.085	6.417GluLys: 6.417 ± 0.083	6.82GluLeu: 6.82 ± 0.089	2.365GluMet: 2.365 ± 0.048	4.215GluAsn: 4.215 ± 0.069	1.827GluPro: 1.827 ± 0.046	3.787GluGln: 3.787 ± 0.067	3.245GluArg: 3.245 ± 0.057	3.562GluSer: 3.562 ± 0.064	3.968GluThr: 3.968 ± 0.062	5.104GluVal: 5.104 ± 0.082	0.98GluTrp: 0.98 ± 0.033	2.353GluTyr: 2.353 ± 0.051	0.0GluXaa: 0.0 ± 0.0
Phe	2.788PheAla: 2.788 ± 0.058	0.311PheCys: 0.311 ± 0.017	2.555PheAsp: 2.555 ± 0.056	2.862PheGlu: 2.862 ± 0.051	2.306PhePhe: 2.306 ± 0.059	2.87PheGly: 2.87 ± 0.058	1.023PheHis: 1.023 ± 0.034	4.166PheIle: 4.166 ± 0.082	2.41PheLys: 2.41 ± 0.048	4.461PheLeu: 4.461 ± 0.083	1.223PheMet: 1.223 ± 0.038	1.984PheAsn: 1.984 ± 0.05	1.649PhePro: 1.649 ± 0.047	1.931PheGln: 1.931 ± 0.04	1.66PheArg: 1.66 ± 0.046	3.147PheSer: 3.147 ± 0.06	2.544PheThr: 2.544 ± 0.06	2.969PheVal: 2.969 ± 0.061	0.498PheTrp: 0.498 ± 0.026	1.904PheTyr: 1.904 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	3.967GlyAla: 3.967 ± 0.064	0.5GlyCys: 0.5 ± 0.024	3.163GlyAsp: 3.163 ± 0.061	4.182GlyGlu: 4.182 ± 0.066	3.129GlyPhe: 3.129 ± 0.059	6.093GlyGly: 6.093 ± 0.091	1.174GlyHis: 1.174 ± 0.036	5.623GlyIle: 5.623 ± 0.08	4.408GlyLys: 4.408 ± 0.07	5.857GlyLeu: 5.857 ± 0.094	1.995GlyMet: 1.995 ± 0.045	2.801GlyAsn: 2.801 ± 0.058	1.483GlyPro: 1.483 ± 0.04	2.014GlyGln: 2.014 ± 0.047	2.302GlyArg: 2.302 ± 0.048	3.702GlySer: 3.702 ± 0.062	3.498GlyThr: 3.498 ± 0.079	4.329GlyVal: 4.329 ± 0.081	0.675GlyTrp: 0.675 ± 0.028	2.709GlyTyr: 2.709 ± 0.054	0.0GlyXaa: 0.0 ± 0.0
His	1.333HisAla: 1.333 ± 0.036	0.21HisCys: 0.21 ± 0.016	1.103HisAsp: 1.103 ± 0.036	1.303HisGlu: 1.303 ± 0.041	1.13HisPhe: 1.13 ± 0.038	1.308HisGly: 1.308 ± 0.043	0.774HisHis: 0.774 ± 0.033	1.687HisIle: 1.687 ± 0.048	1.088HisLys: 1.088 ± 0.036	2.157HisLeu: 2.157 ± 0.042	0.54HisMet: 0.54 ± 0.024	0.886HisAsn: 0.886 ± 0.03	1.117HisPro: 1.117 ± 0.036	1.118HisGln: 1.118 ± 0.034	0.904HisArg: 0.904 ± 0.03	1.195HisSer: 1.195 ± 0.031	1.058HisThr: 1.058 ± 0.031	1.423HisVal: 1.423 ± 0.041	0.211HisTrp: 0.211 ± 0.014	1.035HisTyr: 1.035 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.163IleAla: 6.163 ± 0.089	0.623IleCys: 0.623 ± 0.025	5.143IleAsp: 5.143 ± 0.07	6.234IleGlu: 6.234 ± 0.089	3.648IlePhe: 3.648 ± 0.068	5.967IleGly: 5.967 ± 0.088	1.83IleHis: 1.83 ± 0.048	7.061IleIle: 7.061 ± 0.109	5.223IleLys: 5.223 ± 0.08	7.571IleLeu: 7.571 ± 0.098	2.038IleMet: 2.038 ± 0.046	3.972IleAsn: 3.972 ± 0.06	3.449IlePro: 3.449 ± 0.062	3.289IleGln: 3.289 ± 0.068	3.127IleArg: 3.127 ± 0.065	5.469IleSer: 5.469 ± 0.072	4.839IleThr: 4.839 ± 0.064	5.523IleVal: 5.523 ± 0.09	0.748IleTrp: 0.748 ± 0.032	2.827IleTyr: 2.827 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	4.207LysAla: 4.207 ± 0.071	0.313LysCys: 0.313 ± 0.02	4.007LysAsp: 4.007 ± 0.067	6.811LysGlu: 6.811 ± 0.092	1.899LysPhe: 1.899 ± 0.04	3.886LysGly: 3.886 ± 0.066	1.433LysHis: 1.433 ± 0.037	4.898LysIle: 4.898 ± 0.073	5.8LysLys: 5.8 ± 0.093	5.574LysLeu: 5.574 ± 0.077	2.162LysMet: 2.162 ± 0.05	3.628LysAsn: 3.628 ± 0.071	2.025LysPro: 2.025 ± 0.046	3.748LysGln: 3.748 ± 0.074	3.184LysArg: 3.184 ± 0.067	3.577LysSer: 3.577 ± 0.06	3.424LysThr: 3.424 ± 0.063	4.403LysVal: 4.403 ± 0.066	0.898LysTrp: 0.898 ± 0.027	2.413LysTyr: 2.413 ± 0.056	0.0LysXaa: 0.0 ± 0.0
Leu	6.659LeuAla: 6.659 ± 0.085	0.589LeuCys: 0.589 ± 0.026	5.107LeuAsp: 5.107 ± 0.067	6.879LeuGlu: 6.879 ± 0.098	4.786LeuPhe: 4.786 ± 0.09	5.381LeuGly: 5.381 ± 0.087	1.985LeuHis: 1.985 ± 0.05	7.823LeuIle: 7.823 ± 0.118	6.355LeuLys: 6.355 ± 0.098	9.923LeuLeu: 9.923 ± 0.116	2.443LeuMet: 2.443 ± 0.048	4.517LeuAsn: 4.517 ± 0.064	3.726LeuPro: 3.726 ± 0.054	3.818LeuGln: 3.818 ± 0.059	3.408LeuArg: 3.408 ± 0.065	6.483LeuSer: 6.483 ± 0.075	5.454LeuThr: 5.454 ± 0.068	5.657LeuVal: 5.657 ± 0.091	0.865LeuTrp: 0.865 ± 0.033	3.264LeuTyr: 3.264 ± 0.069	0.0LeuXaa: 0.0 ± 0.0
Met	1.899MetAla: 1.899 ± 0.044	0.134MetCys: 0.134 ± 0.012	1.554MetAsp: 1.554 ± 0.037	2.258MetGlu: 2.258 ± 0.052	1.067MetPhe: 1.067 ± 0.033	1.537MetGly: 1.537 ± 0.039	0.466MetHis: 0.466 ± 0.025	2.475MetIle: 2.475 ± 0.05	2.442MetLys: 2.442 ± 0.048	2.573MetLeu: 2.573 ± 0.045	0.826MetMet: 0.826 ± 0.029	1.618MetAsn: 1.618 ± 0.042	0.984MetPro: 0.984 ± 0.036	1.042MetGln: 1.042 ± 0.03	1.07MetArg: 1.07 ± 0.035	1.581MetSer: 1.581 ± 0.037	1.562MetThr: 1.562 ± 0.043	1.834MetVal: 1.834 ± 0.048	0.191MetTrp: 0.191 ± 0.014	0.824MetTyr: 0.824 ± 0.03	0.0MetXaa: 0.0 ± 0.0
Asn	2.73AsnAla: 2.73 ± 0.055	0.27AsnCys: 0.27 ± 0.018	2.81AsnAsp: 2.81 ± 0.06	3.84AsnGlu: 3.84 ± 0.063	1.905AsnPhe: 1.905 ± 0.043	3.446AsnGly: 3.446 ± 0.066	1.282AsnHis: 1.282 ± 0.038	4.065AsnIle: 4.065 ± 0.067	3.439AsnLys: 3.439 ± 0.06	3.936AsnLeu: 3.936 ± 0.065	1.349AsnMet: 1.349 ± 0.032	2.599AsnAsn: 2.599 ± 0.065	1.996AsnPro: 1.996 ± 0.049	2.708AsnGln: 2.708 ± 0.055	2.197AsnArg: 2.197 ± 0.048	2.594AsnSer: 2.594 ± 0.051	2.417AsnThr: 2.417 ± 0.05	3.008AsnVal: 3.008 ± 0.062	0.66AsnTrp: 0.66 ± 0.027	2.018AsnTyr: 2.018 ± 0.048	0.0AsnXaa: 0.0 ± 0.0
Pro	2.06ProAla: 2.06 ± 0.042	0.204ProCys: 0.204 ± 0.013	1.925ProAsp: 1.925 ± 0.047	2.779ProGlu: 2.779 ± 0.054	1.877ProPhe: 1.877 ± 0.039	2.016ProGly: 2.016 ± 0.05	0.702ProHis: 0.702 ± 0.025	3.017ProIle: 3.017 ± 0.051	1.954ProLys: 1.954 ± 0.049	3.224ProLeu: 3.224 ± 0.063	0.808ProMet: 0.808 ± 0.032	1.776ProAsn: 1.776 ± 0.045	1.38ProPro: 1.38 ± 0.04	1.066ProGln: 1.066 ± 0.036	0.981ProArg: 0.981 ± 0.034	2.149ProSer: 2.149 ± 0.043	1.94ProThr: 1.94 ± 0.046	2.478ProVal: 2.478 ± 0.051	0.363ProTrp: 0.363 ± 0.024	1.388ProTyr: 1.388 ± 0.041	0.0ProXaa: 0.0 ± 0.0
Gln	2.855GlnAla: 2.855 ± 0.051	0.21GlnCys: 0.21 ± 0.016	1.984GlnAsp: 1.984 ± 0.047	3.36GlnGlu: 3.36 ± 0.065	1.881GlnPhe: 1.881 ± 0.043	2.136GlnGly: 2.136 ± 0.045	1.011GlnHis: 1.011 ± 0.037	3.232GlnIle: 3.232 ± 0.056	2.992GlnLys: 2.992 ± 0.059	4.864GlnLeu: 4.864 ± 0.073	1.233GlnMet: 1.233 ± 0.037	1.956GlnAsn: 1.956 ± 0.051	1.361GlnPro: 1.361 ± 0.043	2.603GlnGln: 2.603 ± 0.067	1.558GlnArg: 1.558 ± 0.042	2.53GlnSer: 2.53 ± 0.049	2.392GlnThr: 2.392 ± 0.042	2.748GlnVal: 2.748 ± 0.058	0.488GlnTrp: 0.488 ± 0.024	1.632GlnTyr: 1.632 ± 0.039	0.0GlnXaa: 0.0 ± 0.0
Arg	2.118ArgAla: 2.118 ± 0.048	0.274ArgCys: 0.274 ± 0.018	2.04ArgAsp: 2.04 ± 0.047	2.975ArgGlu: 2.975 ± 0.065	1.954ArgPhe: 1.954 ± 0.044	2.07ArgGly: 2.07 ± 0.048	0.822ArgHis: 0.822 ± 0.029	3.186ArgIle: 3.186 ± 0.058	3.075ArgLys: 3.075 ± 0.061	3.757ArgLeu: 3.757 ± 0.062	1.275ArgMet: 1.275 ± 0.039	1.996ArgAsn: 1.996 ± 0.045	1.143ArgPro: 1.143 ± 0.034	1.576ArgGln: 1.576 ± 0.041	1.655ArgArg: 1.655 ± 0.04	2.214ArgSer: 2.214 ± 0.045	1.925ArgThr: 1.925 ± 0.042	2.426ArgVal: 2.426 ± 0.047	0.413ArgTrp: 0.413 ± 0.021	1.638ArgTyr: 1.638 ± 0.043	0.0ArgXaa: 0.0 ± 0.0
Ser	3.357SerAla: 3.357 ± 0.063	0.389SerCys: 0.389 ± 0.02	3.252SerAsp: 3.252 ± 0.056	4.098SerGlu: 4.098 ± 0.068	3.236SerPhe: 3.236 ± 0.063	3.854SerGly: 3.854 ± 0.063	1.201SerHis: 1.201 ± 0.037	5.34SerIle: 5.34 ± 0.07	3.779SerLys: 3.779 ± 0.063	5.981SerLeu: 5.981 ± 0.087	1.694SerMet: 1.694 ± 0.046	2.952SerAsn: 2.952 ± 0.062	1.881SerPro: 1.881 ± 0.043	2.178SerGln: 2.178 ± 0.051	2.126SerArg: 2.126 ± 0.053	3.856SerSer: 3.856 ± 0.075	3.119SerThr: 3.119 ± 0.054	3.865SerVal: 3.865 ± 0.058	0.634SerTrp: 0.634 ± 0.024	2.398SerTyr: 2.398 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	3.589ThrAla: 3.589 ± 0.062	0.387ThrCys: 0.387 ± 0.023	3.084ThrAsp: 3.084 ± 0.055	3.806ThrGlu: 3.806 ± 0.077	2.684ThrPhe: 2.684 ± 0.054	3.752ThrGly: 3.752 ± 0.071	1.033ThrHis: 1.033 ± 0.035	4.946ThrIle: 4.946 ± 0.072	3.415ThrLys: 3.415 ± 0.058	5.068ThrLeu: 5.068 ± 0.077	1.378ThrMet: 1.378 ± 0.04	2.936ThrAsn: 2.936 ± 0.053	2.033ThrPro: 2.033 ± 0.043	1.585ThrGln: 1.585 ± 0.046	1.715ThrArg: 1.715 ± 0.044	3.188ThrSer: 3.188 ± 0.057	3.092ThrThr: 3.092 ± 0.061	3.901ThrVal: 3.901 ± 0.078	0.575ThrTrp: 0.575 ± 0.024	2.083ThrTyr: 2.083 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	4.472ValAla: 4.472 ± 0.068	0.461ValCys: 0.461 ± 0.022	3.691ValAsp: 3.691 ± 0.061	4.901ValGlu: 4.901 ± 0.074	2.944ValPhe: 2.944 ± 0.059	3.971ValGly: 3.971 ± 0.069	1.27ValHis: 1.27 ± 0.034	5.803ValIle: 5.803 ± 0.078	4.296ValLys: 4.296 ± 0.066	6.127ValLeu: 6.127 ± 0.085	1.747ValMet: 1.747 ± 0.041	3.25ValAsn: 3.25 ± 0.063	2.271ValPro: 2.271 ± 0.048	2.333ValGln: 2.333 ± 0.053	2.285ValArg: 2.285 ± 0.049	4.17ValSer: 4.17 ± 0.065	4.035ValThr: 4.035 ± 0.076	4.428ValVal: 4.428 ± 0.08	0.589ValTrp: 0.589 ± 0.025	2.281ValTyr: 2.281 ± 0.052	0.0ValXaa: 0.0 ± 0.0
Trp	0.52TrpAla: 0.52 ± 0.023	0.084TrpCys: 0.084 ± 0.008	0.555TrpAsp: 0.555 ± 0.025	0.71TrpGlu: 0.71 ± 0.029	0.522TrpPhe: 0.522 ± 0.024	0.576TrpGly: 0.576 ± 0.027	0.263TrpHis: 0.263 ± 0.015	0.935TrpIle: 0.935 ± 0.033	0.805TrpLys: 0.805 ± 0.029	1.2TrpLeu: 1.2 ± 0.033	0.439TrpMet: 0.439 ± 0.021	0.63TrpAsn: 0.63 ± 0.027	0.267TrpPro: 0.267 ± 0.017	0.538TrpGln: 0.538 ± 0.025	0.435TrpArg: 0.435 ± 0.023	0.629TrpSer: 0.629 ± 0.026	0.554TrpThr: 0.554 ± 0.025	0.59TrpVal: 0.59 ± 0.025	0.166TrpTrp: 0.166 ± 0.013	0.373TrpTyr: 0.373 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.128TyrAla: 2.128 ± 0.048	0.285TyrCys: 0.285 ± 0.018	2.154TyrAsp: 2.154 ± 0.047	2.281TyrGlu: 2.281 ± 0.051	1.964TyrPhe: 1.964 ± 0.047	2.447TyrGly: 2.447 ± 0.057	1.057TyrHis: 1.057 ± 0.031	2.819TyrIle: 2.819 ± 0.059	2.041TyrLys: 2.041 ± 0.043	3.909TyrLeu: 3.909 ± 0.067	0.933TyrMet: 0.933 ± 0.031	1.721TyrAsn: 1.721 ± 0.042	1.453TyrPro: 1.453 ± 0.036	2.502TyrGln: 2.502 ± 0.053	1.792TyrArg: 1.792 ± 0.047	2.123TyrSer: 2.123 ± 0.046	1.826TyrThr: 1.826 ± 0.042	2.22TyrVal: 2.22 ± 0.048	0.448TyrTrp: 0.448 ± 0.022	1.67TyrTyr: 1.67 ± 0.045	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4450 proteins (993814 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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