Tursiops truncatus papillomavirus 1
Average proteome isoelectric point is 6.28
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B4XYE1|B4XYE1_9PAPI Replication protein E1 OS=Tursiops truncatus papillomavirus 1 OX=936059 GN=E1 PE=3 SV=1
MM1 pKa = 7.72 ANTNPCTIIEE11 pKa = 4.2 LCEE14 pKa = 3.97 QFDD17 pKa = 4.33 LDD19 pKa = 4.41 LYY21 pKa = 11.12 EE22 pKa = 6.14 LFICCVFCRR31 pKa = 11.84 KK32 pKa = 9.46 VLEE35 pKa = 5.49 DD36 pKa = 3.37 IEE38 pKa = 4.55 KK39 pKa = 8.32 WSCRR43 pKa = 11.84 NRR45 pKa = 11.84 EE46 pKa = 3.91 INVVWKK52 pKa = 10.59 KK53 pKa = 9.2 EE54 pKa = 3.74 YY55 pKa = 9.95 PYY57 pKa = 11.04 AVCCKK62 pKa = 9.45 CLEE65 pKa = 4.17 VQAILDD71 pKa = 3.61 LLRR74 pKa = 11.84 YY75 pKa = 9.03 FEE77 pKa = 4.87 RR78 pKa = 11.84 SGSAKK83 pKa = 9.43 TVEE86 pKa = 4.3 EE87 pKa = 4.33 DD88 pKa = 2.94 TGKK91 pKa = 9.79 PLGDD95 pKa = 3.49 LRR97 pKa = 11.84 VRR99 pKa = 11.84 CYY101 pKa = 11.13 GCYY104 pKa = 9.93 KK105 pKa = 10.44 PLTATEE111 pKa = 4.32 KK112 pKa = 10.49 LFHH115 pKa = 8.0 VEE117 pKa = 3.85 DD118 pKa = 3.51 QRR120 pKa = 11.84 PFTKK124 pKa = 10.23 VCNQWRR130 pKa = 11.84 GICTNCQYY138 pKa = 11.11 LPPRR142 pKa = 11.84 LLYY145 pKa = 10.88 YY146 pKa = 10.38 FFTIAGRR153 pKa = 11.84 TVRR156 pKa = 11.84 SPLPGLTWGFDD167 pKa = 3.64 PPPANLSEE175 pKa = 4.61 SEE177 pKa = 4.26 SSSWTTTTTSSDD189 pKa = 3.25 TSSQTSSSVTSGRR202 pKa = 11.84 RR203 pKa = 11.84 DD204 pKa = 3.58 DD205 pKa = 4.3 NLSDD209 pKa = 4.15 AEE211 pKa = 4.41 SNEE214 pKa = 3.82 NDD216 pKa = 3.74 DD217 pKa = 4.49 EE218 pKa = 4.61 NPEE221 pKa = 4.0 VLII224 pKa = 5.03
Molecular weight: 25.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.594
IPC2_protein 4.813
IPC_protein 4.711
Toseland 4.558
ProMoST 4.8
Dawson 4.647
Bjellqvist 4.8
Wikipedia 4.507
Rodwell 4.546
Grimsley 4.469
Solomon 4.647
Lehninger 4.596
Nozaki 4.749
DTASelect 4.889
Thurlkill 4.558
EMBOSS 4.52
Sillero 4.825
Patrickios 3.007
IPC_peptide 4.647
IPC2_peptide 4.813
IPC2.peptide.svr19 4.787
Protein with the highest isoelectric point:
>tr|B4XYE5|B4XYE5_9PAPI Minor capsid protein L2 OS=Tursiops truncatus papillomavirus 1 OX=936059 GN=L2 PE=3 SV=1
LL1 pKa = 6.99 LVCILFVLFPLCIFVIKK18 pKa = 10.51 SCMHH22 pKa = 6.73 PASLLYY28 pKa = 9.98 IYY30 pKa = 10.31 II31 pKa = 4.39
Molecular weight: 3.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.168
IPC2_protein 6.854
IPC_protein 7.249
Toseland 6.737
ProMoST 7.556
Dawson 7.805
Bjellqvist 7.951
Wikipedia 7.717
Rodwell 7.761
Grimsley 6.707
Solomon 7.98
Lehninger 8.009
Nozaki 8.141
DTASelect 7.878
Thurlkill 7.907
EMBOSS 8.053
Sillero 8.244
Patrickios 0.006
IPC_peptide 7.98
IPC2_peptide 7.907
IPC2.peptide.svr19 7.924
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2481
31
639
354.4
39.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.804 ± 0.515
2.66 ± 0.718
5.804 ± 0.763
5.844 ± 0.649
3.708 ± 0.334
6.651 ± 0.585
2.499 ± 0.675
4.635 ± 0.47
5.038 ± 0.854
8.787 ± 0.655
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.894 ± 0.462
3.99 ± 0.553
7.134 ± 0.931
3.869 ± 0.78
5.361 ± 0.609
8.303 ± 0.91
6.933 ± 0.782
6.489 ± 0.357
1.693 ± 0.248
2.902 ± 0.162
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here