Bat polyomavirus 5a

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Alphapolyomavirus; Dobsonia moluccensis polyomavirus 1

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D5ZZ35|A0A0D5ZZ35_9POLY Capsid protein VP1 OS=Bat polyomavirus 5a OX=1623687 GN=VP1 PE=3 SV=1
MM1 pKa = 7.37APKK4 pKa = 10.38RR5 pKa = 11.84KK6 pKa = 9.72GGSSKK11 pKa = 11.35GEE13 pKa = 3.93TKK15 pKa = 10.4SCSNICSTKK24 pKa = 10.12CPVVNPVPKK33 pKa = 10.43LLVKK37 pKa = 10.82GGIQVLSVKK46 pKa = 9.56TGPDD50 pKa = 3.54SITQIEE56 pKa = 4.95AYY58 pKa = 10.06LNPRR62 pKa = 11.84MGQPPASSTLGPGYY76 pKa = 9.07SEE78 pKa = 4.25KK79 pKa = 11.14VGVATYY85 pKa = 9.61WGDD88 pKa = 3.39TDD90 pKa = 5.09KK91 pKa = 11.24PLANHH96 pKa = 6.99LPMYY100 pKa = 10.02SCARR104 pKa = 11.84INLPMLNEE112 pKa = 4.9DD113 pKa = 3.86MTCDD117 pKa = 3.43TLQMWEE123 pKa = 4.04AVSVKK128 pKa = 9.81TEE130 pKa = 4.11VVGTTSLLDD139 pKa = 3.1SHH141 pKa = 6.69YY142 pKa = 10.65AGKK145 pKa = 10.55RR146 pKa = 11.84MYY148 pKa = 10.51SDD150 pKa = 3.44YY151 pKa = 11.34GIALPIEE158 pKa = 4.67GTSLHH163 pKa = 6.1FFSVGGEE170 pKa = 3.95PLDD173 pKa = 4.2LQGLVANGKK182 pKa = 9.84AIYY185 pKa = 7.17PTTLAVINNMTEE197 pKa = 4.06KK198 pKa = 10.89NQVLQQKK205 pKa = 10.33AKK207 pKa = 10.98AKK209 pKa = 9.68LLKK212 pKa = 10.76DD213 pKa = 3.07GTYY216 pKa = 9.74PVEE219 pKa = 3.95MWGPDD224 pKa = 3.26PSRR227 pKa = 11.84NEE229 pKa = 3.41NTRR232 pKa = 11.84YY233 pKa = 10.37YY234 pKa = 10.51GTFTGGQTTPPVLQFTNTVTTVLLDD259 pKa = 3.84EE260 pKa = 5.12NGVGPLCKK268 pKa = 10.13GDD270 pKa = 3.88GLFIASVDD278 pKa = 3.81VIGFYY283 pKa = 10.37TDD285 pKa = 2.56SSGYY289 pKa = 8.56MYY291 pKa = 10.98YY292 pKa = 10.34RR293 pKa = 11.84GLPRR297 pKa = 11.84YY298 pKa = 9.74FNITLRR304 pKa = 11.84KK305 pKa = 9.42RR306 pKa = 11.84NVKK309 pKa = 10.03NPYY312 pKa = 9.3PVTSLLSSLFNNMIPKK328 pKa = 9.91LQGQPMEE335 pKa = 4.42GEE337 pKa = 4.05EE338 pKa = 4.43GQVEE342 pKa = 4.38EE343 pKa = 4.18VRR345 pKa = 11.84VYY347 pKa = 10.83EE348 pKa = 4.26GLEE351 pKa = 3.96GLPGDD356 pKa = 4.44PDD358 pKa = 2.97MDD360 pKa = 3.57RR361 pKa = 11.84YY362 pKa = 10.82VDD364 pKa = 4.43QFGQDD369 pKa = 3.12KK370 pKa = 10.25TIIPSLRR377 pKa = 11.84TNDD380 pKa = 3.51EE381 pKa = 4.01LL382 pKa = 5.69

Molecular weight: 41.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D5ZYM7|A0A0D5ZYM7_9POLY Large T antigen OS=Bat polyomavirus 5a OX=1623687 PE=4 SV=1
MM1 pKa = 8.0DD2 pKa = 4.39KK3 pKa = 11.03FMDD6 pKa = 3.86RR7 pKa = 11.84EE8 pKa = 4.06EE9 pKa = 4.49LKK11 pKa = 10.7EE12 pKa = 3.99LCEE15 pKa = 4.09LLNIPAHH22 pKa = 6.64CYY24 pKa = 10.85GNLPMMKK31 pKa = 9.53INYY34 pKa = 9.78KK35 pKa = 10.14KK36 pKa = 9.33MCLIYY41 pKa = 10.7HH42 pKa = 6.89PDD44 pKa = 3.28KK45 pKa = 11.45GGDD48 pKa = 3.42VAKK51 pKa = 8.75MQRR54 pKa = 11.84MNEE57 pKa = 3.86LWQKK61 pKa = 10.39LQDD64 pKa = 3.73GVINARR70 pKa = 11.84DD71 pKa = 3.8EE72 pKa = 4.8GPVSRR77 pKa = 11.84WFWEE81 pKa = 4.08YY82 pKa = 10.63QGQTLRR88 pKa = 11.84EE89 pKa = 3.87FLGPDD94 pKa = 3.21FNKK97 pKa = 10.11RR98 pKa = 11.84FCKK101 pKa = 10.34VFPTCLYY108 pKa = 10.53ASKK111 pKa = 10.27EE112 pKa = 3.93FCFCVCCLLNKK123 pKa = 7.32QHH125 pKa = 7.21KK126 pKa = 8.13IYY128 pKa = 10.29KK129 pKa = 9.17VKK131 pKa = 10.59RR132 pKa = 11.84EE133 pKa = 3.92KK134 pKa = 10.81NAA136 pKa = 3.36

Molecular weight: 16.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1474

136

647

368.5

41.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.834 ± 1.534

2.307 ± 0.752

5.02 ± 0.174

6.716 ± 0.509

4.071 ± 0.695

6.513 ± 1.16

1.628 ± 0.261

4.953 ± 0.517

7.123 ± 1.216

10.176 ± 0.419

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.917 ± 0.508

5.563 ± 0.656

5.834 ± 0.528

4.478 ± 0.203

4.206 ± 0.472

5.97 ± 0.756

5.563 ± 0.815

6.174 ± 0.527

1.153 ± 0.324

3.799 ± 0.453
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski