Amino acid dipepetide frequency for Nitrosomonas sp. Nm166

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.246AlaAla: 9.246 ± 0.139
0.988AlaCys: 0.988 ± 0.036
4.884AlaAsp: 4.884 ± 0.084
5.65AlaGlu: 5.65 ± 0.086
3.339AlaPhe: 3.339 ± 0.073
6.744AlaGly: 6.744 ± 0.111
2.087AlaHis: 2.087 ± 0.047
6.194AlaIle: 6.194 ± 0.082
4.093AlaLys: 4.093 ± 0.074
9.627AlaLeu: 9.627 ± 0.135
2.32AlaMet: 2.32 ± 0.052
3.276AlaAsn: 3.276 ± 0.073
3.135AlaPro: 3.135 ± 0.07
3.864AlaGln: 3.864 ± 0.089
4.773AlaArg: 4.773 ± 0.07
5.147AlaSer: 5.147 ± 0.078
4.555AlaThr: 4.555 ± 0.093
5.845AlaVal: 5.845 ± 0.084
1.146AlaTrp: 1.146 ± 0.047
2.51AlaTyr: 2.51 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.883CysAla: 0.883 ± 0.033
0.157CysCys: 0.157 ± 0.015
0.48CysAsp: 0.48 ± 0.025
0.558CysGlu: 0.558 ± 0.029
0.401CysPhe: 0.401 ± 0.02
0.87CysGly: 0.87 ± 0.029
0.372CysHis: 0.372 ± 0.024
0.696CysIle: 0.696 ± 0.027
0.384CysLys: 0.384 ± 0.021
0.948CysLeu: 0.948 ± 0.037
0.229CysMet: 0.229 ± 0.016
0.392CysAsn: 0.392 ± 0.018
0.436CysPro: 0.436 ± 0.024
0.366CysGln: 0.366 ± 0.019
0.545CysArg: 0.545 ± 0.026
0.609CysSer: 0.609 ± 0.027
0.474CysThr: 0.474 ± 0.024
0.605CysVal: 0.605 ± 0.027
0.121CysTrp: 0.121 ± 0.011
0.34CysTyr: 0.34 ± 0.019
0.0CysXaa: 0.0 ± 0.0
Asp
4.624AspAla: 4.624 ± 0.087
0.509AspCys: 0.509 ± 0.021
2.914AspAsp: 2.914 ± 0.086
3.412AspGlu: 3.412 ± 0.071
2.478AspPhe: 2.478 ± 0.064
4.4AspGly: 4.4 ± 0.167
1.337AspHis: 1.337 ± 0.041
3.703AspIle: 3.703 ± 0.055
2.624AspLys: 2.624 ± 0.064
5.588AspLeu: 5.588 ± 0.109
1.259AspMet: 1.259 ± 0.035
2.036AspAsn: 2.036 ± 0.051
2.495AspPro: 2.495 ± 0.065
2.196AspGln: 2.196 ± 0.055
2.785AspArg: 2.785 ± 0.061
3.025AspSer: 3.025 ± 0.062
2.815AspThr: 2.815 ± 0.066
3.632AspVal: 3.632 ± 0.105
0.871AspTrp: 0.871 ± 0.037
1.835AspTyr: 1.835 ± 0.044
0.0AspXaa: 0.0 ± 0.0
Glu
5.385GluAla: 5.385 ± 0.082
0.456GluCys: 0.456 ± 0.025
2.581GluAsp: 2.581 ± 0.062
3.568GluGlu: 3.568 ± 0.065
2.32GluPhe: 2.32 ± 0.057
3.175GluGly: 3.175 ± 0.063
1.45GluHis: 1.45 ± 0.046
4.847GluIle: 4.847 ± 0.086
3.709GluLys: 3.709 ± 0.079
6.225GluLeu: 6.225 ± 0.102
1.584GluMet: 1.584 ± 0.043
2.687GluAsn: 2.687 ± 0.06
2.125GluPro: 2.125 ± 0.057
3.019GluGln: 3.019 ± 0.068
3.618GluArg: 3.618 ± 0.079
3.409GluSer: 3.409 ± 0.07
3.21GluThr: 3.21 ± 0.052
3.636GluVal: 3.636 ± 0.065
0.718GluTrp: 0.718 ± 0.03
1.6GluTyr: 1.6 ± 0.043
0.0GluXaa: 0.0 ± 0.0
Phe
3.442PheAla: 3.442 ± 0.066
0.484PheCys: 0.484 ± 0.022
2.984PheAsp: 2.984 ± 0.083
2.358PheGlu: 2.358 ± 0.051
1.844PhePhe: 1.844 ± 0.053
3.214PheGly: 3.214 ± 0.09
0.915PheHis: 0.915 ± 0.034
2.72PheIle: 2.72 ± 0.064
1.607PheLys: 1.607 ± 0.045
3.733PheLeu: 3.733 ± 0.068
0.897PheMet: 0.897 ± 0.038
1.827PheAsn: 1.827 ± 0.047
1.661PhePro: 1.661 ± 0.046
1.278PheGln: 1.278 ± 0.036
2.169PheArg: 2.169 ± 0.066
3.084PheSer: 3.084 ± 0.065
2.266PheThr: 2.266 ± 0.056
2.413PheVal: 2.413 ± 0.051
0.519PheTrp: 0.519 ± 0.027
1.296PheTyr: 1.296 ± 0.043
0.0PheXaa: 0.0 ± 0.0
Gly
5.612GlyAla: 5.612 ± 0.123
0.807GlyCys: 0.807 ± 0.03
4.16GlyAsp: 4.16 ± 0.158
3.969GlyGlu: 3.969 ± 0.066
3.864GlyPhe: 3.864 ± 0.159
5.242GlyGly: 5.242 ± 0.119
1.721GlyHis: 1.721 ± 0.046
5.297GlyIle: 5.297 ± 0.075
4.196GlyLys: 4.196 ± 0.079
6.665GlyLeu: 6.665 ± 0.105
1.856GlyMet: 1.856 ± 0.056
3.04GlyAsn: 3.04 ± 0.084
1.878GlyPro: 1.878 ± 0.059
2.537GlyGln: 2.537 ± 0.053
3.747GlyArg: 3.747 ± 0.069
4.54GlySer: 4.54 ± 0.11
3.821GlyThr: 3.821 ± 0.079
4.531GlyVal: 4.531 ± 0.083
1.041GlyTrp: 1.041 ± 0.039
2.381GlyTyr: 2.381 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.233HisAla: 2.233 ± 0.049
0.31HisCys: 0.31 ± 0.017
1.255HisAsp: 1.255 ± 0.037
1.387HisGlu: 1.387 ± 0.037
1.049HisPhe: 1.049 ± 0.037
1.794HisGly: 1.794 ± 0.048
0.849HisHis: 0.849 ± 0.038
1.652HisIle: 1.652 ± 0.042
0.94HisLys: 0.94 ± 0.031
2.618HisLeu: 2.618 ± 0.062
0.551HisMet: 0.551 ± 0.026
0.905HisAsn: 0.905 ± 0.031
1.398HisPro: 1.398 ± 0.041
1.184HisGln: 1.184 ± 0.031
1.304HisArg: 1.304 ± 0.037
1.404HisSer: 1.404 ± 0.043
1.26HisThr: 1.26 ± 0.039
1.443HisVal: 1.443 ± 0.045
0.354HisTrp: 0.354 ± 0.019
0.872HisTyr: 0.872 ± 0.029
0.0HisXaa: 0.0 ± 0.0
Ile
6.776IleAla: 6.776 ± 0.097
0.703IleCys: 0.703 ± 0.028
4.184IleAsp: 4.184 ± 0.071
4.635IleGlu: 4.635 ± 0.074
2.581IlePhe: 2.581 ± 0.055
4.931IleGly: 4.931 ± 0.088
1.59IleHis: 1.59 ± 0.045
4.375IleIle: 4.375 ± 0.087
3.468IleLys: 3.468 ± 0.076
6.457IleLeu: 6.457 ± 0.098
1.369IleMet: 1.369 ± 0.043
3.245IleAsn: 3.245 ± 0.062
3.274IlePro: 3.274 ± 0.07
2.636IleGln: 2.636 ± 0.059
3.636IleArg: 3.636 ± 0.064
4.52IleSer: 4.52 ± 0.075
4.118IleThr: 4.118 ± 0.107
4.371IleVal: 4.371 ± 0.071
0.727IleTrp: 0.727 ± 0.032
1.809IleTyr: 1.809 ± 0.047
0.0IleXaa: 0.0 ± 0.0
Lys
4.075LysAla: 4.075 ± 0.068
0.335LysCys: 0.335 ± 0.02
2.285LysAsp: 2.285 ± 0.051
2.909LysGlu: 2.909 ± 0.074
1.472LysPhe: 1.472 ± 0.044
2.652LysGly: 2.652 ± 0.059
1.158LysHis: 1.158 ± 0.036
3.631LysIle: 3.631 ± 0.074
2.862LysLys: 2.862 ± 0.072
5.177LysLeu: 5.177 ± 0.086
1.271LysMet: 1.271 ± 0.037
2.389LysAsn: 2.389 ± 0.065
2.347LysPro: 2.347 ± 0.054
2.506LysGln: 2.506 ± 0.06
2.801LysArg: 2.801 ± 0.067
2.917LysSer: 2.917 ± 0.063
2.676LysThr: 2.676 ± 0.055
2.762LysVal: 2.762 ± 0.06
0.534LysTrp: 0.534 ± 0.028
1.171LysTyr: 1.171 ± 0.04
0.0LysXaa: 0.0 ± 0.0
Leu
9.663LeuAla: 9.663 ± 0.118
1.045LeuCys: 1.045 ± 0.036
5.988LeuAsp: 5.988 ± 0.128
5.906LeuGlu: 5.906 ± 0.093
4.024LeuPhe: 4.024 ± 0.077
6.626LeuGly: 6.626 ± 0.099
2.437LeuHis: 2.437 ± 0.06
7.09LeuIle: 7.09 ± 0.101
5.065LeuLys: 5.065 ± 0.085
11.256LeuLeu: 11.256 ± 0.161
2.459LeuMet: 2.459 ± 0.049
4.504LeuAsn: 4.504 ± 0.079
5.249LeuPro: 5.249 ± 0.083
4.41LeuGln: 4.41 ± 0.08
5.692LeuArg: 5.692 ± 0.092
7.381LeuSer: 7.381 ± 0.105
6.013LeuThr: 6.013 ± 0.09
6.147LeuVal: 6.147 ± 0.084
1.158LeuTrp: 1.158 ± 0.041
2.449LeuTyr: 2.449 ± 0.053
0.0LeuXaa: 0.0 ± 0.0
Met
2.106MetAla: 2.106 ± 0.042
0.165MetCys: 0.165 ± 0.013
1.156MetAsp: 1.156 ± 0.043
1.308MetGlu: 1.308 ± 0.041
0.712MetPhe: 0.712 ± 0.028
1.572MetGly: 1.572 ± 0.046
0.601MetHis: 0.601 ± 0.026
1.542MetIle: 1.542 ± 0.044
1.286MetLys: 1.286 ± 0.041
2.686MetLeu: 2.686 ± 0.061
0.648MetMet: 0.648 ± 0.03
1.218MetAsn: 1.218 ± 0.034
1.231MetPro: 1.231 ± 0.037
1.268MetGln: 1.268 ± 0.037
1.457MetArg: 1.457 ± 0.04
1.525MetSer: 1.525 ± 0.043
1.357MetThr: 1.357 ± 0.04
1.531MetVal: 1.531 ± 0.045
0.169MetTrp: 0.169 ± 0.014
0.401MetTyr: 0.401 ± 0.022
0.0MetXaa: 0.0 ± 0.0
Asn
3.53AsnAla: 3.53 ± 0.068
0.427AsnCys: 0.427 ± 0.022
2.209AsnAsp: 2.209 ± 0.062
2.302AsnGlu: 2.302 ± 0.052
1.736AsnPhe: 1.736 ± 0.043
3.561AsnGly: 3.561 ± 0.152
1.017AsnHis: 1.017 ± 0.033
2.921AsnIle: 2.921 ± 0.059
1.988AsnLys: 1.988 ± 0.06
4.43AsnLeu: 4.43 ± 0.078
0.908AsnMet: 0.908 ± 0.032
1.875AsnAsn: 1.875 ± 0.053
2.433AsnPro: 2.433 ± 0.053
1.944AsnGln: 1.944 ± 0.057
2.194AsnArg: 2.194 ± 0.054
2.511AsnSer: 2.511 ± 0.067
2.24AsnThr: 2.24 ± 0.062
2.37AsnVal: 2.37 ± 0.057
0.57AsnTrp: 0.57 ± 0.026
1.284AsnTyr: 1.284 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
3.847ProAla: 3.847 ± 0.074
0.394ProCys: 0.394 ± 0.02
2.893ProAsp: 2.893 ± 0.061
3.252ProGlu: 3.252 ± 0.06
1.803ProPhe: 1.803 ± 0.048
3.163ProGly: 3.163 ± 0.066
1.035ProHis: 1.035 ± 0.039
2.744ProIle: 2.744 ± 0.059
1.799ProLys: 1.799 ± 0.044
4.246ProLeu: 4.246 ± 0.078
0.964ProMet: 0.964 ± 0.029
1.772ProAsn: 1.772 ± 0.052
1.886ProPro: 1.886 ± 0.06
1.768ProGln: 1.768 ± 0.045
1.853ProArg: 1.853 ± 0.048
2.508ProSer: 2.508 ± 0.057
2.006ProThr: 2.006 ± 0.049
3.446ProVal: 3.446 ± 0.063
0.555ProTrp: 0.555 ± 0.029
1.319ProTyr: 1.319 ± 0.038
0.0ProXaa: 0.0 ± 0.0
Gln
4.202GlnAla: 4.202 ± 0.07
0.355GlnCys: 0.355 ± 0.019
1.826GlnAsp: 1.826 ± 0.048
2.384GlnGlu: 2.384 ± 0.064
1.578GlnPhe: 1.578 ± 0.044
2.686GlnGly: 2.686 ± 0.067
1.168GlnHis: 1.168 ± 0.036
3.042GlnIle: 3.042 ± 0.066
2.113GlnLys: 2.113 ± 0.053
4.806GlnLeu: 4.806 ± 0.086
1.015GlnMet: 1.015 ± 0.033
1.657GlnAsn: 1.657 ± 0.049
1.883GlnPro: 1.883 ± 0.049
2.502GlnGln: 2.502 ± 0.059
2.664GlnArg: 2.664 ± 0.064
2.65GlnSer: 2.65 ± 0.061
2.158GlnThr: 2.158 ± 0.052
2.811GlnVal: 2.811 ± 0.062
0.58GlnTrp: 0.58 ± 0.026
1.152GlnTyr: 1.152 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
4.261ArgAla: 4.261 ± 0.074
0.478ArgCys: 0.478 ± 0.021
2.721ArgAsp: 2.721 ± 0.055
3.38ArgGlu: 3.38 ± 0.078
2.466ArgPhe: 2.466 ± 0.057
3.126ArgGly: 3.126 ± 0.063
1.415ArgHis: 1.415 ± 0.034
4.175ArgIle: 4.175 ± 0.076
2.667ArgLys: 2.667 ± 0.062
6.272ArgLeu: 6.272 ± 0.107
1.475ArgMet: 1.475 ± 0.042
2.436ArgAsn: 2.436 ± 0.053
2.004ArgPro: 2.004 ± 0.049
2.499ArgGln: 2.499 ± 0.06
3.191ArgArg: 3.191 ± 0.078
3.06ArgSer: 3.06 ± 0.055
2.531ArgThr: 2.531 ± 0.056
3.348ArgVal: 3.348 ± 0.075
0.839ArgTrp: 0.839 ± 0.034
1.948ArgTyr: 1.948 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
5.487SerAla: 5.487 ± 0.092
0.635SerCys: 0.635 ± 0.025
3.372SerAsp: 3.372 ± 0.062
3.5SerGlu: 3.5 ± 0.063
2.449SerPhe: 2.449 ± 0.048
5.729SerGly: 5.729 ± 0.129
1.552SerHis: 1.552 ± 0.042
3.961SerIle: 3.961 ± 0.07
2.618SerLys: 2.618 ± 0.049
6.489SerLeu: 6.489 ± 0.088
1.494SerMet: 1.494 ± 0.04
2.792SerAsn: 2.792 ± 0.078
2.498SerPro: 2.498 ± 0.053
2.474SerGln: 2.474 ± 0.057
3.193SerArg: 3.193 ± 0.061
4.765SerSer: 4.765 ± 0.131
3.157SerThr: 3.157 ± 0.073
4.086SerVal: 4.086 ± 0.069
0.761SerTrp: 0.761 ± 0.035
1.924SerTyr: 1.924 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
4.951ThrAla: 4.951 ± 0.085
0.5ThrCys: 0.5 ± 0.025
3.044ThrAsp: 3.044 ± 0.076
3.004ThrGlu: 3.004 ± 0.067
1.927ThrPhe: 1.927 ± 0.043
4.472ThrGly: 4.472 ± 0.095
1.422ThrHis: 1.422 ± 0.048
3.468ThrIle: 3.468 ± 0.057
1.936ThrLys: 1.936 ± 0.047
6.121ThrLeu: 6.121 ± 0.083
1.06ThrMet: 1.06 ± 0.037
1.885ThrAsn: 1.885 ± 0.05
2.793ThrPro: 2.793 ± 0.062
2.312ThrGln: 2.312 ± 0.058
2.681ThrArg: 2.681 ± 0.059
3.157ThrSer: 3.157 ± 0.092
2.932ThrThr: 2.932 ± 0.079
3.589ThrVal: 3.589 ± 0.079
0.591ThrTrp: 0.591 ± 0.027
1.451ThrTyr: 1.451 ± 0.06
0.0ThrXaa: 0.0 ± 0.0
Val
5.729ValAla: 5.729 ± 0.078
0.644ValCys: 0.644 ± 0.027
3.47ValAsp: 3.47 ± 0.059
3.68ValGlu: 3.68 ± 0.066
2.794ValPhe: 2.794 ± 0.068
4.138ValGly: 4.138 ± 0.086
1.36ValHis: 1.36 ± 0.04
4.919ValIle: 4.919 ± 0.083
2.86ValLys: 2.86 ± 0.058
6.548ValLeu: 6.548 ± 0.081
1.716ValMet: 1.716 ± 0.046
2.997ValAsn: 2.997 ± 0.079
2.671ValPro: 2.671 ± 0.053
2.203ValGln: 2.203 ± 0.055
3.242ValArg: 3.242 ± 0.06
4.277ValSer: 4.277 ± 0.076
3.721ValThr: 3.721 ± 0.065
4.63ValVal: 4.63 ± 0.082
0.719ValTrp: 0.719 ± 0.032
1.575ValTyr: 1.575 ± 0.046
0.0ValXaa: 0.0 ± 0.0
Trp
0.816TrpAla: 0.816 ± 0.032
0.152TrpCys: 0.152 ± 0.014
0.583TrpAsp: 0.583 ± 0.028
0.638TrpGlu: 0.638 ± 0.031
0.565TrpPhe: 0.565 ± 0.028
0.789TrpGly: 0.789 ± 0.038
0.393TrpHis: 0.393 ± 0.022
0.861TrpIle: 0.861 ± 0.031
0.582TrpLys: 0.582 ± 0.032
1.702TrpLeu: 1.702 ± 0.052
0.338TrpMet: 0.338 ± 0.019
0.546TrpAsn: 0.546 ± 0.028
0.49TrpPro: 0.49 ± 0.024
0.778TrpGln: 0.778 ± 0.038
0.832TrpArg: 0.832 ± 0.033
0.698TrpSer: 0.698 ± 0.031
0.497TrpThr: 0.497 ± 0.027
0.805TrpVal: 0.805 ± 0.027
0.211TrpTrp: 0.211 ± 0.02
0.354TrpTyr: 0.354 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.554TyrAla: 2.554 ± 0.056
0.322TyrCys: 0.322 ± 0.019
1.547TyrAsp: 1.547 ± 0.05
1.523TyrGlu: 1.523 ± 0.049
1.307TyrPhe: 1.307 ± 0.04
2.021TyrGly: 2.021 ± 0.051
0.884TyrHis: 0.884 ± 0.04
1.566TyrIle: 1.566 ± 0.049
1.134TyrLys: 1.134 ± 0.037
2.967TyrLeu: 2.967 ± 0.066
0.527TyrMet: 0.527 ± 0.023
0.974TyrAsn: 0.974 ± 0.041
1.368TyrPro: 1.368 ± 0.047
1.488TyrGln: 1.488 ± 0.038
1.915TyrArg: 1.915 ± 0.047
1.748TyrSer: 1.748 ± 0.05
1.49TyrThr: 1.49 ± 0.048
1.888TyrVal: 1.888 ± 0.06
0.446TyrTrp: 0.446 ± 0.022
0.982TyrTyr: 0.982 ± 0.038
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3014 proteins (915179 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski