Water chestnut soymovirus 1
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A172PC86|A0A172PC86_9VIRU Reverse transcriptase OS=Water chestnut soymovirus 1 OX=1848040 GN=WCSV-1gp5 PE=4 SV=1
MM1 pKa = 7.75 SNLEE5 pKa = 4.26 TLNKK9 pKa = 10.31 DD10 pKa = 3.22 IEE12 pKa = 4.63 STEE15 pKa = 3.76 QMIEE19 pKa = 3.95 YY20 pKa = 9.95 LQDD23 pKa = 3.1 EE24 pKa = 5.09 LEE26 pKa = 5.26 RR27 pKa = 11.84 ITQTLVVLRR36 pKa = 11.84 EE37 pKa = 4.11 TLQSLNNEE45 pKa = 4.23 RR46 pKa = 11.84 NDD48 pKa = 3.63 LEE50 pKa = 4.14 ASRR53 pKa = 11.84 EE54 pKa = 3.87 EE55 pKa = 4.4 LEE57 pKa = 4.13 NEE59 pKa = 3.94 EE60 pKa = 4.07 YY61 pKa = 10.92 LAIEE65 pKa = 5.13 DD66 pKa = 4.35 EE67 pKa = 4.72 LSSHH71 pKa = 7.6 DD72 pKa = 4.03 YY73 pKa = 11.01 YY74 pKa = 11.72 DD75 pKa = 3.59 NLTEE79 pKa = 4.24 EE80 pKa = 4.68 EE81 pKa = 4.82 YY82 pKa = 11.0 EE83 pKa = 3.94 NLMNRR88 pKa = 11.84 LEE90 pKa = 4.05 II91 pKa = 4.69
Molecular weight: 10.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.112
IPC2_protein 3.923
IPC_protein 3.821
Toseland 3.668
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.617
Rodwell 3.656
Grimsley 3.579
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 3.948
Thurlkill 3.681
EMBOSS 3.63
Sillero 3.923
Patrickios 3.083
IPC_peptide 3.757
IPC2_peptide 3.91
IPC2.peptide.svr19 3.857
Protein with the highest isoelectric point:
>tr|A0A172PC90|A0A172PC90_9VIRU Uncharacterized protein OS=Water chestnut soymovirus 1 OX=1848040 GN=WCSV-1gp9 PE=4 SV=1
MM1 pKa = 7.17 TKK3 pKa = 10.39 KK4 pKa = 10.53 KK5 pKa = 10.15 VVNVGKK11 pKa = 10.67 RR12 pKa = 11.84 KK13 pKa = 8.89 QKK15 pKa = 10.45 KK16 pKa = 9.12 LKK18 pKa = 10.26 KK19 pKa = 9.0 EE20 pKa = 3.64 LWRR23 pKa = 11.84 NEE25 pKa = 3.85 QFRR28 pKa = 11.84 GLAQDD33 pKa = 4.33 KK34 pKa = 10.49 GIKK37 pKa = 9.88 CIIRR41 pKa = 11.84 AMVNANHH48 pKa = 5.92 SVKK51 pKa = 10.77 SIVRR55 pKa = 11.84 NVFRR59 pKa = 5.91
Molecular weight: 6.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.185
IPC2_protein 10.087
IPC_protein 11.184
Toseland 11.623
ProMoST 11.959
Dawson 11.637
Bjellqvist 11.477
Wikipedia 11.974
Rodwell 11.915
Grimsley 11.667
Solomon 11.974
Lehninger 11.901
Nozaki 11.608
DTASelect 11.477
Thurlkill 11.608
EMBOSS 12.062
Sillero 11.608
Patrickios 11.652
IPC_peptide 11.989
IPC2_peptide 10.833
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9
0
9
2363
59
680
262.6
30.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.174 ± 0.602
1.523 ± 0.336
4.951 ± 0.289
9.31 ± 1.266
3.512 ± 0.487
4.613 ± 0.426
2.243 ± 0.189
7.871 ± 0.986
10.072 ± 0.844
10.03 ± 0.578
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.497 ± 0.337
6.348 ± 0.805
3.724 ± 0.521
4.994 ± 0.428
4.528 ± 0.567
5.544 ± 0.433
5.459 ± 0.718
5.121 ± 0.341
1.016 ± 0.219
3.47 ± 0.353
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here