Hubei macula-like virus 2
Average proteome isoelectric point is 7.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KJV4|A0A1L3KJV4_9VIRU Uncharacterized protein OS=Hubei macula-like virus 2 OX=1922923 PE=4 SV=1
MM1 pKa = 6.94 NTLLQTAIEE10 pKa = 4.21 HH11 pKa = 6.22 APAIISALPSLLPPSSQSTSTSSDD35 pKa = 3.26 PVHH38 pKa = 7.91 DD39 pKa = 5.4 DD40 pKa = 3.47 RR41 pKa = 11.84 LLLANSALPPAPSTPSHH58 pKa = 6.38 ISSFPSSSSVKK69 pKa = 10.46 VPFQYY74 pKa = 10.99 VWFGLDD80 pKa = 3.15 GTEE83 pKa = 4.65 SKK85 pKa = 10.5 NASITIPEE93 pKa = 4.1 IPSVKK98 pKa = 10.18 KK99 pKa = 10.34 IIAFFRR105 pKa = 11.84 DD106 pKa = 3.33 AKK108 pKa = 10.63 LVNLEE113 pKa = 3.97 AAVYY117 pKa = 8.67 PHH119 pKa = 7.03 FDD121 pKa = 3.85 CLEE124 pKa = 4.01 KK125 pKa = 10.62 PITVDD130 pKa = 4.84 LMWSPSDD137 pKa = 3.68 TVPSGSILNCPGAVCFTVGGQFLSQSGILPCDD169 pKa = 3.84 LNFMNPIIKK178 pKa = 10.41 SPIPYY183 pKa = 8.88 TNHH186 pKa = 6.0 PRR188 pKa = 11.84 LNAKK192 pKa = 9.7 FYY194 pKa = 11.17 KK195 pKa = 10.4 NQTEE199 pKa = 4.33 LQSHH203 pKa = 6.51 RR204 pKa = 11.84 KK205 pKa = 7.15 ATIIIRR211 pKa = 11.84 GIIACAHH218 pKa = 5.19 PTVYY222 pKa = 10.85 AA223 pKa = 4.13
Molecular weight: 24.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.503
IPC2_protein 6.465
IPC_protein 6.678
Toseland 6.707
ProMoST 7.117
Dawson 7.176
Bjellqvist 7.029
Wikipedia 7.146
Rodwell 7.161
Grimsley 6.81
Solomon 7.22
Lehninger 7.234
Nozaki 7.395
DTASelect 7.468
Thurlkill 7.497
EMBOSS 7.541
Sillero 7.6
Patrickios 3.973
IPC_peptide 7.234
IPC2_peptide 7.161
IPC2.peptide.svr19 7.032
Protein with the highest isoelectric point:
>tr|A0A1L3KJV4|A0A1L3KJV4_9VIRU Uncharacterized protein OS=Hubei macula-like virus 2 OX=1922923 PE=4 SV=1
MM1 pKa = 7.65 SSLSNNMQILSNTLHH16 pKa = 7.02 KK17 pKa = 10.71 DD18 pKa = 3.19 ASTHH22 pKa = 6.19 PLLTHH27 pKa = 5.67 TLKK30 pKa = 10.8 NLHH33 pKa = 6.26 TSLHH37 pKa = 5.81 AFPWSLTSEE46 pKa = 4.21 QVEE49 pKa = 4.15 LLVNLGINVSPHH61 pKa = 4.72 GTLPHH66 pKa = 6.16 PHH68 pKa = 7.12 PVHH71 pKa = 6.5 KK72 pKa = 10.63 CIEE75 pKa = 4.12 THH77 pKa = 6.21 VLFNHH82 pKa = 6.3 WNHH85 pKa = 6.76 LAVQPSAVCFMKK97 pKa = 10.38 PSKK100 pKa = 9.85 FAKK103 pKa = 10.19 LSNSNPNFIEE113 pKa = 3.78 LHH115 pKa = 6.05 NYY117 pKa = 9.43 IIDD120 pKa = 3.7 ARR122 pKa = 11.84 DD123 pKa = 3.39 PTRR126 pKa = 11.84 YY127 pKa = 9.35 PSISSSLPTTPYY139 pKa = 11.17 LFIHH143 pKa = 7.15 DD144 pKa = 4.48 ALMFISPNEE153 pKa = 4.19 IIDD156 pKa = 4.46 LFSNCPNLTHH166 pKa = 6.97 VFGTLVLPAEE176 pKa = 4.94 SIHH179 pKa = 6.42 NIPSFYY185 pKa = 10.07 PDD187 pKa = 2.93 IYY189 pKa = 10.43 TYY191 pKa = 10.71 HH192 pKa = 6.89 HH193 pKa = 6.94 EE194 pKa = 4.5 NGNLHH199 pKa = 6.39 YY200 pKa = 9.97 FLEE203 pKa = 5.26 GNPSPHH209 pKa = 5.19 YY210 pKa = 7.7 TQPKK214 pKa = 9.34 DD215 pKa = 3.14 AAIWLTTHH223 pKa = 7.56 TINGPNFSLSVVILEE238 pKa = 4.39 SWLSVHH244 pKa = 7.05 SILITKK250 pKa = 7.11 YY251 pKa = 8.0 QPRR254 pKa = 11.84 VPTYY258 pKa = 8.99 KK259 pKa = 10.25 HH260 pKa = 5.71 YY261 pKa = 10.78 FRR263 pKa = 11.84 IPKK266 pKa = 9.42 TSLLPNPTNLNLPLHH281 pKa = 6.16 SRR283 pKa = 11.84 LVPTEE288 pKa = 3.57 VHH290 pKa = 6.58 ASLFNYY296 pKa = 8.49 VRR298 pKa = 11.84 AVRR301 pKa = 11.84 TLRR304 pKa = 11.84 TSDD307 pKa = 3.08 PSAFIRR313 pKa = 11.84 SLRR316 pKa = 11.84 SKK318 pKa = 10.86 EE319 pKa = 3.8 EE320 pKa = 3.84 YY321 pKa = 9.87 RR322 pKa = 11.84 WVHH325 pKa = 5.97 ASAWDD330 pKa = 3.6 QLVQFALATHH340 pKa = 6.44 NVTSPSLFAFIEE352 pKa = 4.27 TFLRR356 pKa = 11.84 QILNHH361 pKa = 6.63 AKK363 pKa = 9.04 LTFLQFIPNPIHH375 pKa = 7.1 HH376 pKa = 6.91 AFFWLLAKK384 pKa = 10.16 ALSYY388 pKa = 8.97 ITPKK392 pKa = 10.04 PYY394 pKa = 9.56 STIFHH399 pKa = 5.94 VASIISPLLHH409 pKa = 6.29 TYY411 pKa = 8.99 WLFSHH416 pKa = 7.45 PSPPQVILANYY427 pKa = 7.89 NQYY430 pKa = 10.45 FHH432 pKa = 7.83 PDD434 pKa = 3.0 PWLLFADD441 pKa = 4.96 RR442 pKa = 11.84 YY443 pKa = 10.85 SIVAMPKK450 pKa = 9.66 PFLSLTLQTPPPSPTSTPPPQPTQQTQPALPAPPPVQTSNPLNSTPFAPPSSLPPLQTPQSAQPTILSTSTPVLPPIVSPPITTCNNPDD539 pKa = 4.14 PLLTDD544 pKa = 3.8 PTAIGVILPFNQLYY558 pKa = 9.79 PGLSMPSHH566 pKa = 6.2 VCNFPSRR573 pKa = 11.84 LRR575 pKa = 11.84 DD576 pKa = 3.44 PNAPLPSYY584 pKa = 10.48 PITQVCLFDD593 pKa = 4.64 ALSTLLQQPQHH604 pKa = 6.19 NLFQILAQYY613 pKa = 10.61 LPDD616 pKa = 3.82 SLVVGPEE623 pKa = 4.05 EE624 pKa = 4.18 KK625 pKa = 10.11 TSGYY629 pKa = 8.66 STDD632 pKa = 3.74 HH633 pKa = 7.16 LDD635 pKa = 2.94 ILLYY639 pKa = 10.9 HH640 pKa = 6.95 FDD642 pKa = 3.55 QPLIIHH648 pKa = 6.62 SSLGDD653 pKa = 3.62 EE654 pKa = 3.92 PHH656 pKa = 6.6 GPKK659 pKa = 10.16 NSRR662 pKa = 11.84 NLLHH666 pKa = 7.15 LYY668 pKa = 10.03 HH669 pKa = 6.35 FTGHH673 pKa = 6.66 WSAQPPSQPPLRR685 pKa = 11.84 GSGLSPFANAVLSFRR700 pKa = 11.84 CEE702 pKa = 4.2 DD703 pKa = 3.46 NSLLPIANVYY713 pKa = 6.69 VHH715 pKa = 6.49 KK716 pKa = 10.99 VNYY719 pKa = 9.36 HH720 pKa = 4.97 RR721 pKa = 11.84 AKK723 pKa = 10.65 NLSSNLKK730 pKa = 10.1 NEE732 pKa = 3.93 NDD734 pKa = 4.8 GILLTQLKK742 pKa = 10.34 QSVNDD747 pKa = 3.51 NKK749 pKa = 10.76 LLSKK753 pKa = 10.2 LDD755 pKa = 3.64 SMMDD759 pKa = 3.54 NPVSRR764 pKa = 11.84 HH765 pKa = 4.74 VEE767 pKa = 4.32 LIHH770 pKa = 6.66 IAGFPGCGKK779 pKa = 9.18 SHH781 pKa = 7.09 PVASLLKK788 pKa = 10.27 CKK790 pKa = 7.84 TFRR793 pKa = 11.84 NSFRR797 pKa = 11.84 VVVPTVEE804 pKa = 5.63 LRR806 pKa = 11.84 SEE808 pKa = 4.07 WKK810 pKa = 10.42 DD811 pKa = 3.36 KK812 pKa = 11.72 LEE814 pKa = 4.62 LPDD817 pKa = 3.83 FEE819 pKa = 4.53 SWRR822 pKa = 11.84 ISTWEE827 pKa = 3.79 TSLLKK832 pKa = 10.26 LAKK835 pKa = 10.26 VLVIDD840 pKa = 4.86 EE841 pKa = 4.58 VYY843 pKa = 10.82 KK844 pKa = 11.03 LPNGFLDD851 pKa = 4.12 LAVALDD857 pKa = 3.71 PTLQFVILLGDD868 pKa = 4.14 PCQGTYY874 pKa = 10.6 HH875 pKa = 6.6 SLSPHH880 pKa = 5.2 STNHH884 pKa = 6.13 HH885 pKa = 6.83 IYY887 pKa = 10.41 PEE889 pKa = 4.03 TEE891 pKa = 4.0 YY892 pKa = 10.97 LSKK895 pKa = 11.18 YY896 pKa = 9.93 RR897 pKa = 11.84 DD898 pKa = 4.6 FYY900 pKa = 11.32 CLWTHH905 pKa = 6.54 RR906 pKa = 11.84 TPQVIANLLNVRR918 pKa = 11.84 SSSKK922 pKa = 10.89 EE923 pKa = 3.35 NGQISRR929 pKa = 11.84 SNTLNASFPVLATSMAIATHH949 pKa = 6.68 LNQNGFRR956 pKa = 11.84 SITYY960 pKa = 9.9 AGSQGLTLNRR970 pKa = 11.84 LTYY973 pKa = 10.63 LCVDD977 pKa = 4.27 RR978 pKa = 11.84 YY979 pKa = 9.9 ISLMSPGVTLVALTRR994 pKa = 11.84 STKK997 pKa = 10.82 GIIFNGNYY1005 pKa = 10.27 DD1006 pKa = 3.84 LLGNLGYY1013 pKa = 9.53 TNTLLEE1019 pKa = 4.54 SIFRR1023 pKa = 11.84 GEE1025 pKa = 4.67 KK1026 pKa = 9.33 IDD1028 pKa = 3.9 FQHH1031 pKa = 7.23 LFHH1034 pKa = 6.12 QQLRR1038 pKa = 11.84 GSRR1041 pKa = 11.84 IITQPIMSPTDD1052 pKa = 2.99 RR1053 pKa = 11.84 KK1054 pKa = 9.22 MLRR1057 pKa = 11.84 GGAILITKK1065 pKa = 6.86 TAKK1068 pKa = 9.81 IDD1070 pKa = 3.49 INSSIDD1076 pKa = 3.55 FLRR1079 pKa = 11.84 QDD1081 pKa = 3.0 SQLRR1085 pKa = 11.84 SNPEE1089 pKa = 4.63 LIPLHH1094 pKa = 7.06 DD1095 pKa = 4.56 NFYY1098 pKa = 10.5 LPPSRR1103 pKa = 11.84 LVLSQSFSSSLHH1115 pKa = 6.27 PPAKK1119 pKa = 10.17 PNNTPFAPTPFEE1131 pKa = 4.02 PSYY1134 pKa = 10.37 PGYY1137 pKa = 10.0 PFEE1140 pKa = 4.92 TYY1142 pKa = 9.85 FANLPDD1148 pKa = 4.23 PSEE1151 pKa = 4.5 PEE1153 pKa = 4.52 SKK1155 pKa = 10.56 EE1156 pKa = 3.33 KK1157 pKa = 9.74 WYY1159 pKa = 10.59 KK1160 pKa = 10.57 DD1161 pKa = 3.0 KK1162 pKa = 10.99 RR1163 pKa = 11.84 SQQFPILNKK1172 pKa = 9.91 GSPYY1176 pKa = 10.87 SPFPANIVAPIHH1188 pKa = 6.85 DD1189 pKa = 4.4 SKK1191 pKa = 11.46 KK1192 pKa = 10.94 DD1193 pKa = 3.57 STLLPLSINKK1203 pKa = 9.63 RR1204 pKa = 11.84 LRR1206 pKa = 11.84 FRR1208 pKa = 11.84 PDD1210 pKa = 2.81 NKK1212 pKa = 9.99 PYY1214 pKa = 10.59 QITEE1218 pKa = 3.89 LDD1220 pKa = 3.72 HH1221 pKa = 6.72 YY1222 pKa = 10.01 VANHH1226 pKa = 6.85 LFQSYY1231 pKa = 10.48 CEE1233 pKa = 4.19 TLNTTIDD1240 pKa = 3.11 IKK1242 pKa = 11.45 VPFDD1246 pKa = 3.07 AHH1248 pKa = 7.11 LFAEE1252 pKa = 5.28 CINLNDD1258 pKa = 3.76 YY1259 pKa = 10.56 NQLTKK1264 pKa = 9.61 KK1265 pKa = 8.78 TRR1267 pKa = 11.84 ATLVANAYY1275 pKa = 10.2 RR1276 pKa = 11.84 SDD1278 pKa = 3.94 PDD1280 pKa = 3.0 WRR1282 pKa = 11.84 FTFVRR1287 pKa = 11.84 IFSKK1291 pKa = 9.02 TQHH1294 pKa = 6.65 KK1295 pKa = 10.69 INTNSLYY1302 pKa = 10.29 TSWKK1306 pKa = 9.38 ACQTLALMNDD1316 pKa = 4.87 LIVLTLGPVKK1326 pKa = 10.46 KK1327 pKa = 10.05 YY1328 pKa = 10.43 QRR1330 pKa = 11.84 LINDD1334 pKa = 4.47 PLRR1337 pKa = 11.84 KK1338 pKa = 9.92 SNIFTYY1344 pKa = 10.39 GGKK1347 pKa = 8.06 TPFDD1351 pKa = 3.75 LSSFCQNHH1359 pKa = 5.55 FTPKK1363 pKa = 9.21 STVANDD1369 pKa = 3.13 YY1370 pKa = 8.79 TAFDD1374 pKa = 3.61 QSQLGEE1380 pKa = 4.29 SLCLEE1385 pKa = 4.11 VLKK1388 pKa = 10.21 MKK1390 pKa = 10.5 RR1391 pKa = 11.84 LSIPNNFIEE1400 pKa = 5.02 LHH1402 pKa = 5.47 LTLKK1406 pKa = 10.76 RR1407 pKa = 11.84 NLYY1410 pKa = 9.88 CQFGPLTCMRR1420 pKa = 11.84 FTGEE1424 pKa = 3.96 PGTWDD1429 pKa = 4.66 DD1430 pKa = 3.68 NTDD1433 pKa = 3.48 YY1434 pKa = 11.21 NLSVLFTLFQITNQAVLVSGDD1455 pKa = 4.04 DD1456 pKa = 3.57 SCINPIPPFNPRR1468 pKa = 11.84 WNSIKK1473 pKa = 10.54 KK1474 pKa = 10.2 LLLLKK1479 pKa = 10.7 FKK1481 pKa = 11.18 LEE1483 pKa = 4.0 YY1484 pKa = 9.89 TKK1486 pKa = 10.77 FPIFCGYY1493 pKa = 10.36 YY1494 pKa = 9.0 VGKK1497 pKa = 10.44 AGALRR1502 pKa = 11.84 APTALAHH1509 pKa = 6.73 KK1510 pKa = 9.43 IHH1512 pKa = 6.6 NALADD1517 pKa = 3.86 DD1518 pKa = 4.93 SISDD1522 pKa = 3.34 KK1523 pKa = 10.91 HH1524 pKa = 7.7 LSYY1527 pKa = 10.89 LSEE1530 pKa = 3.92 FAIGHH1535 pKa = 5.44 SLGEE1539 pKa = 4.15 QLWTLLPHH1547 pKa = 6.67 HH1548 pKa = 6.53 EE1549 pKa = 4.4 VLAQSALFDD1558 pKa = 3.66 YY1559 pKa = 10.3 FCRR1562 pKa = 11.84 KK1563 pKa = 7.68 TSKK1566 pKa = 10.47 KK1567 pKa = 10.36 NKK1569 pKa = 8.66 LLLYY1573 pKa = 9.88 VGPPKK1578 pKa = 10.13 QEE1580 pKa = 4.05 HH1581 pKa = 6.57 LNLLTFDD1588 pKa = 3.58 IEE1590 pKa = 4.67 SYY1592 pKa = 10.19 QALRR1596 pKa = 11.84 QYY1598 pKa = 10.74 QRR1600 pKa = 11.84 ANLLSKK1606 pKa = 10.23 HH1607 pKa = 5.48 QPPPLAPTLEE1617 pKa = 4.38 PVLQQIFNNN1626 pKa = 3.93
Molecular weight: 183.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.814
IPC2_protein 8.024
IPC_protein 7.819
Toseland 7.761
ProMoST 8.39
Dawson 8.595
Bjellqvist 8.814
Wikipedia 8.595
Rodwell 8.653
Grimsley 7.907
Solomon 8.624
Lehninger 8.624
Nozaki 8.902
DTASelect 8.609
Thurlkill 8.668
EMBOSS 8.77
Sillero 8.916
Patrickios 4.075
IPC_peptide 8.624
IPC2_peptide 7.688
IPC2.peptide.svr19 7.836
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1849
223
1626
924.5
104.04
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.408 ± 1.064
1.46 ± 0.312
4.056 ± 0.008
3.029 ± 0.135
5.138 ± 0.261
3.191 ± 0.158
4.327 ± 0.474
6.057 ± 1.163
4.651 ± 0.067
12.655 ± 1.473
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
0.973 ± 0.149
6.165 ± 0.672
9.627 ± 0.454
4.056 ± 0.366
3.407 ± 0.465
9.519 ± 1.213
6.923 ± 0.258
4.489 ± 0.356
1.19 ± 0.117
3.678 ± 0.573
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here