Longibacter salinarum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidetes Order II. Incertae sedis; Rhodothermaceae; Longibacter

Average proteome isoelectric point is 5.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3409 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A8CUI9|A0A2A8CUI9_9BACT Uncharacterized protein OS=Longibacter salinarum OX=1850348 GN=CRI94_14310 PE=4 SV=1
MM1 pKa = 7.8PLADD5 pKa = 3.78SLPRR9 pKa = 11.84LRR11 pKa = 11.84SFSFLLVLTLALIATGCDD29 pKa = 3.22SGGSAMDD36 pKa = 4.98DD37 pKa = 3.93DD38 pKa = 4.42MDD40 pKa = 5.32SEE42 pKa = 4.74NEE44 pKa = 4.1DD45 pKa = 3.69AVTTLADD52 pKa = 3.69VVNDD56 pKa = 3.43IANLSTLGAALEE68 pKa = 4.18ATGQDD73 pKa = 3.03EE74 pKa = 4.43TLADD78 pKa = 4.03EE79 pKa = 4.5NTTFTVFAPANAAFDD94 pKa = 4.04NVDD97 pKa = 3.77VEE99 pKa = 4.84SLTNNPDD106 pKa = 2.89LTTRR110 pKa = 11.84LLNYY114 pKa = 9.89HH115 pKa = 5.83VVAGEE120 pKa = 4.07ALTASDD126 pKa = 4.01LEE128 pKa = 4.52GRR130 pKa = 11.84QSVTTVEE137 pKa = 4.34GQEE140 pKa = 4.06LPIASEE146 pKa = 4.03NGTLTVGGIPVTQADD161 pKa = 3.81VEE163 pKa = 4.32ADD165 pKa = 3.03NGVAHH170 pKa = 6.9VIDD173 pKa = 4.64GVLVPDD179 pKa = 4.86SFPQRR184 pKa = 11.84ISYY187 pKa = 10.55DD188 pKa = 3.02LAAQSNGGALPNGVDD203 pKa = 3.47GTVTFWEE210 pKa = 4.57AGDD213 pKa = 3.82TQTLVTLSLTDD224 pKa = 3.93GPTGTTVSHH233 pKa = 7.37PAHH236 pKa = 6.16IHH238 pKa = 5.93NNTASEE244 pKa = 4.37GGSIAIYY251 pKa = 10.17LSPIDD256 pKa = 3.65GTNANSANDD265 pKa = 3.97GTSARR270 pKa = 11.84LVDD273 pKa = 3.91RR274 pKa = 11.84PFDD277 pKa = 3.64EE278 pKa = 5.16LATFNGYY285 pKa = 9.69VNIHH289 pKa = 6.17EE290 pKa = 4.64SVANLDD296 pKa = 3.8NVVSQGNIGANATGTFGAGLALLDD320 pKa = 3.98NPQTTTYY327 pKa = 10.4PLQANANSGSVAPSGIPGEE346 pKa = 4.0ITFRR350 pKa = 11.84EE351 pKa = 4.46LTTDD355 pKa = 3.65LTLATVRR362 pKa = 11.84LDD364 pKa = 3.29VDD366 pKa = 3.67GDD368 pKa = 3.87GQYY371 pKa = 11.43EE372 pKa = 4.1DD373 pKa = 5.22DD374 pKa = 3.88ATGADD379 pKa = 3.93VSHH382 pKa = 7.62PGHH385 pKa = 6.01IHH387 pKa = 6.68ANSASEE393 pKa = 4.3GGDD396 pKa = 2.87IQYY399 pKa = 10.84YY400 pKa = 10.23LSPIDD405 pKa = 3.77GTDD408 pKa = 3.13PAARR412 pKa = 11.84SSQIIDD418 pKa = 3.79LSFGDD423 pKa = 3.88LTSFDD428 pKa = 4.14GYY430 pKa = 11.57VNIHH434 pKa = 6.3EE435 pKa = 4.58SAANLQEE442 pKa = 4.29IVSQGNIGANEE453 pKa = 4.2GTGTSSPDD461 pKa = 3.21VTVTLTNVGASAWEE475 pKa = 3.91VANVEE480 pKa = 4.32GATGVAASGEE490 pKa = 4.4NPTLTLTVGTRR501 pKa = 11.84YY502 pKa = 10.18RR503 pKa = 11.84IEE505 pKa = 4.17NNGGSAHH512 pKa = 7.07PLGFQNANSDD522 pKa = 3.75YY523 pKa = 10.94LLNQGGSGSLEE534 pKa = 3.84GDD536 pKa = 2.89SDD538 pKa = 3.69INYY541 pKa = 9.54VEE543 pKa = 4.81DD544 pKa = 3.55SQGITFTYY552 pKa = 8.94TQTLADD558 pKa = 3.35AVATYY563 pKa = 10.42RR564 pKa = 11.84CTIHH568 pKa = 8.08ASMEE572 pKa = 4.2GTVQTGSS579 pKa = 3.1

Molecular weight: 59.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A8CTI7|A0A2A8CTI7_9BACT DNA ligase-associated DEXH box helicase OS=Longibacter salinarum OX=1850348 GN=CRI94_16375 PE=4 SV=1
II1 pKa = 7.12EE2 pKa = 4.87RR3 pKa = 11.84AICQQLPPLRR13 pKa = 11.84KK14 pKa = 7.24TRR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84NLTRR22 pKa = 11.84FCAGLYY28 pKa = 8.63EE29 pKa = 4.52AEE31 pKa = 4.55HH32 pKa = 5.36VHH34 pKa = 6.16LPKK37 pKa = 10.34IADD40 pKa = 3.78RR41 pKa = 11.84CFGRR45 pKa = 11.84AQQASKK51 pKa = 9.25TRR53 pKa = 11.84RR54 pKa = 11.84LRR56 pKa = 11.84RR57 pKa = 11.84FLANEE62 pKa = 3.84AVRR65 pKa = 11.84PGDD68 pKa = 3.06WYY70 pKa = 11.18RR71 pKa = 11.84PVARR75 pKa = 11.84SLLKK79 pKa = 10.41AAAAAGPVRR88 pKa = 11.84LLIDD92 pKa = 3.66TLHH95 pKa = 6.03LTGEE99 pKa = 4.14RR100 pKa = 11.84RR101 pKa = 11.84LLVAAVAFRR110 pKa = 11.84RR111 pKa = 11.84RR112 pKa = 11.84ALPVLWRR119 pKa = 11.84VHH121 pKa = 5.15ATSGVTGQDD130 pKa = 3.2LQRR133 pKa = 11.84CFVHH137 pKa = 6.75EE138 pKa = 4.34LAALVPEE145 pKa = 4.19EE146 pKa = 4.8AEE148 pKa = 4.29VIVIGDD154 pKa = 4.03GEE156 pKa = 4.18FHH158 pKa = 6.85CVSLLQAVQKK168 pKa = 10.94AGWTYY173 pKa = 10.37CVRR176 pKa = 11.84LHH178 pKa = 7.27ADD180 pKa = 3.23TFVRR184 pKa = 11.84AFVRR188 pKa = 11.84EE189 pKa = 4.22DD190 pKa = 3.15VV191 pKa = 3.53

Molecular weight: 21.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3409

0

3409

1207722

34

3789

354.3

38.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.681 ± 0.042

0.633 ± 0.013

7.101 ± 0.037

6.783 ± 0.046

3.671 ± 0.029

7.769 ± 0.043

2.127 ± 0.028

4.873 ± 0.028

2.62 ± 0.032

9.225 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.021

2.907 ± 0.033

4.994 ± 0.026

3.466 ± 0.024

7.186 ± 0.043

6.62 ± 0.043

6.195 ± 0.037

7.912 ± 0.039

1.259 ± 0.018

2.794 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski