Bacillus phage PBC2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A218KBS7|A0A218KBS7_9CAUD Uncharacterized protein OS=Bacillus phage PBC2 OX=1675029 GN=PBC2_024 PE=4 SV=1
MM1 pKa = 6.57WTWIFIGGFIALIIVLILSGMEE23 pKa = 4.04HH24 pKa = 6.96GSGSGGSSRR33 pKa = 11.84GGSSSSGSSYY43 pKa = 11.3DD44 pKa = 4.57YY45 pKa = 11.65GDD47 pKa = 4.45DD48 pKa = 5.02DD49 pKa = 4.71DD50 pKa = 4.0WW51 pKa = 6.5

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A218KBS2|A0A218KBS2_9CAUD Uncharacterized protein OS=Bacillus phage PBC2 OX=1675029 GN=PBC2_026 PE=4 SV=1
MM1 pKa = 7.33FMLGFVKK8 pKa = 9.8FFIFMFIIFLSVVVSCKK25 pKa = 9.74AKK27 pKa = 8.71SWRR30 pKa = 11.84RR31 pKa = 11.84LTITTPITFLLLMIVEE47 pKa = 4.5RR48 pKa = 11.84LFF50 pKa = 4.0

Molecular weight: 6.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

251

0

251

49663

49

2304

197.9

22.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.537 ± 0.317

0.858 ± 0.064

6.594 ± 0.183

8.131 ± 0.236

4.239 ± 0.125

6.262 ± 0.201

1.865 ± 0.109

7.019 ± 0.168

9.142 ± 0.182

7.714 ± 0.142

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.067 ± 0.106

5.809 ± 0.153

2.227 ± 0.111

3.363 ± 0.197

3.999 ± 0.113

5.719 ± 0.199

5.767 ± 0.232

6.766 ± 0.124

1.244 ± 0.085

4.68 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski