Camellia sinensis var. sinensis
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 30052 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S4DFN4|A0A4S4DFN4_CAMSI Uncharacterized protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_020291 PE=4 SV=1
MM1 pKa = 7.75 AAFLYY6 pKa = 10.48 FDD8 pKa = 6.13 LIINYY13 pKa = 8.65 KK14 pKa = 10.54 DD15 pKa = 3.25 KK16 pKa = 11.09 VGRR19 pKa = 11.84 IYY21 pKa = 11.03 DD22 pKa = 3.67 VDD24 pKa = 3.99 PNMYY28 pKa = 10.19 CYY30 pKa = 10.13 IDD32 pKa = 3.77 ALKK35 pKa = 10.82 DD36 pKa = 3.35 VTEE39 pKa = 4.53 TVLSHH44 pKa = 6.75 IDD46 pKa = 3.45 CSEE49 pKa = 4.48 AIAITLHH56 pKa = 7.01 YY57 pKa = 10.7 DD58 pKa = 3.23 MPGTEE63 pKa = 4.08 NKK65 pKa = 10.18 RR66 pKa = 11.84 LIEE69 pKa = 4.28 NDD71 pKa = 3.69 LDD73 pKa = 3.96 VLDD76 pKa = 4.32 MFYY79 pKa = 10.82 VQCRR83 pKa = 11.84 SKK85 pKa = 9.93 TINLYY90 pKa = 10.71 VDD92 pKa = 3.46 IAYY95 pKa = 10.48 CIGKK99 pKa = 9.76 EE100 pKa = 3.99 DD101 pKa = 3.84 GEE103 pKa = 4.48 GRR105 pKa = 11.84 NDD107 pKa = 3.29 VGDD110 pKa = 3.85 NNGGDD115 pKa = 4.0 GNGGVHH121 pKa = 6.41 GVHH124 pKa = 6.56 TIEE127 pKa = 3.77 VDD129 pKa = 3.08 EE130 pKa = 5.23 EE131 pKa = 4.06 YY132 pKa = 10.89 RR133 pKa = 11.84 EE134 pKa = 4.15 DD135 pKa = 3.59 NVYY138 pKa = 10.82 GFSDD142 pKa = 4.81 EE143 pKa = 4.75 DD144 pKa = 4.2 RR145 pKa = 11.84 DD146 pKa = 3.71 WNASVDD152 pKa = 4.01 GQHH155 pKa = 6.27 EE156 pKa = 4.58 LNGTVSLSDD165 pKa = 3.56 EE166 pKa = 4.68 DD167 pKa = 4.73 NEE169 pKa = 5.16 EE170 pKa = 4.23 SNEE173 pKa = 3.89 DD174 pKa = 4.29 DD175 pKa = 4.68 GLSDD179 pKa = 3.79 YY180 pKa = 11.25 QSGDD184 pKa = 3.26 DD185 pKa = 3.55 EE186 pKa = 4.6 VRR188 pKa = 11.84 YY189 pKa = 10.41 SSSDD193 pKa = 3.42 DD194 pKa = 3.45 EE195 pKa = 4.61 FAGG198 pKa = 4.09
Molecular weight: 22.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.722
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.973
Dawson 3.808
Bjellqvist 3.961
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.757
Nozaki 3.91
DTASelect 4.164
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.935
Patrickios 0.795
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|A0A4S4D3Q4|A0A4S4D3Q4_CAMSI Uncharacterized protein OS=Camellia sinensis var. sinensis OX=542762 GN=TEA_009666 PE=4 SV=1
MM1 pKa = 7.23 SAVAKK6 pKa = 10.36 ASKK9 pKa = 10.48 NVFATGYY16 pKa = 7.64 YY17 pKa = 8.85 AHH19 pKa = 7.34 RR20 pKa = 11.84 RR21 pKa = 11.84 STAPPFVLAAAPPQHH36 pKa = 6.15 RR37 pKa = 11.84 TTVRR41 pKa = 11.84 PRR43 pKa = 11.84 RR44 pKa = 11.84 STAAAPHH51 pKa = 6.33 HH52 pKa = 6.66 RR53 pKa = 11.84 SSSPQHH59 pKa = 6.22 RR60 pKa = 11.84 RR61 pKa = 11.84 STAPPFVLAAAPPQHH76 pKa = 6.15 RR77 pKa = 11.84 TTVRR81 pKa = 11.84 PRR83 pKa = 11.84 RR84 pKa = 11.84 STAAAPHH91 pKa = 6.33 HH92 pKa = 6.66 RR93 pKa = 11.84 SSSPQHH99 pKa = 6.22 RR100 pKa = 11.84 RR101 pKa = 11.84 STAPPFVLAAAPPQHH116 pKa = 6.15 RR117 pKa = 11.84 TTVRR121 pKa = 11.84 PRR123 pKa = 11.84 RR124 pKa = 11.84 STAAAPHH131 pKa = 6.33 HH132 pKa = 6.66 RR133 pKa = 11.84 SSSPQHH139 pKa = 6.22 RR140 pKa = 11.84 RR141 pKa = 11.84 STAPPFVLAAAPPQHH156 pKa = 6.15 RR157 pKa = 11.84 TTVRR161 pKa = 11.84 PRR163 pKa = 11.84 RR164 pKa = 11.84 STAAAPHH171 pKa = 6.33 HH172 pKa = 6.66 RR173 pKa = 11.84 SSSPQHH179 pKa = 6.22 RR180 pKa = 11.84 RR181 pKa = 11.84 STAPPFVLAAAPPQHH196 pKa = 6.15 RR197 pKa = 11.84 TTVRR201 pKa = 11.84 PRR203 pKa = 11.84 RR204 pKa = 11.84 STAAAPHH211 pKa = 6.33 HH212 pKa = 6.66 RR213 pKa = 11.84 SSSPQHH219 pKa = 6.22 RR220 pKa = 11.84 RR221 pKa = 11.84 STAPPFVLAAAPPQHH236 pKa = 6.15 RR237 pKa = 11.84 TTVRR241 pKa = 11.84 PRR243 pKa = 11.84 RR244 pKa = 11.84 STAAAPHH251 pKa = 6.33 HH252 pKa = 6.66 RR253 pKa = 11.84 SSSPQHH259 pKa = 6.22 RR260 pKa = 11.84 RR261 pKa = 11.84 STAPPFVLAAAPPQHH276 pKa = 6.15 RR277 pKa = 11.84 TTVRR281 pKa = 11.84 PRR283 pKa = 11.84 RR284 pKa = 11.84 STAAAPHH291 pKa = 6.33 HH292 pKa = 6.66 RR293 pKa = 11.84 SSSPQHH299 pKa = 6.22 RR300 pKa = 11.84 RR301 pKa = 11.84 STAPPFVLAAAPPQYY316 pKa = 9.25 RR317 pKa = 11.84 TTVRR321 pKa = 11.84 PRR323 pKa = 11.84 RR324 pKa = 11.84 STAAAPHH331 pKa = 6.07 HH332 pKa = 6.49 RR333 pKa = 11.84 SPFGFAGIWGKK344 pKa = 10.67 EE345 pKa = 3.54 IGFTDD350 pKa = 3.79 LGIWII355 pKa = 4.82
Molecular weight: 38.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.509
IPC2_protein 11.316
IPC_protein 12.764
Toseland 12.925
ProMoST 13.422
Dawson 12.925
Bjellqvist 12.925
Wikipedia 13.408
Rodwell 12.442
Grimsley 12.969
Solomon 13.422
Lehninger 13.32
Nozaki 12.925
DTASelect 12.925
Thurlkill 12.925
EMBOSS 13.422
Sillero 12.925
Patrickios 12.149
IPC_peptide 13.422
IPC2_peptide 12.413
IPC2.peptide.svr19 9.197
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
30052
0
30052
13452360
40
5832
447.6
49.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.438 ± 0.012
1.951 ± 0.007
5.186 ± 0.01
6.349 ± 0.015
4.299 ± 0.009
6.666 ± 0.025
2.439 ± 0.007
5.439 ± 0.01
5.872 ± 0.014
10.019 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.483 ± 0.006
4.589 ± 0.011
4.894 ± 0.014
3.636 ± 0.009
5.145 ± 0.01
8.98 ± 0.018
4.95 ± 0.009
6.61 ± 0.01
1.314 ± 0.005
2.742 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here