Streptococcus phage phi-SsuHCJ31_comEC
Average proteome isoelectric point is 6.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A7G3MSW1|A0A7G3MSW1_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuHCJ31_comEC OX=2664398 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.52 LKK4 pKa = 10.66 DD5 pKa = 3.35 ILEE8 pKa = 4.65 LGTYY12 pKa = 9.75 GYY14 pKa = 11.27 NPDD17 pKa = 3.86 CKK19 pKa = 11.03 VEE21 pKa = 4.12 IFNMDD26 pKa = 3.22 NFEE29 pKa = 4.08 EE30 pKa = 4.3 RR31 pKa = 11.84 LEE33 pKa = 4.14 NEE35 pKa = 4.24 GFDD38 pKa = 5.31 EE39 pKa = 5.2 ILIPQNEE46 pKa = 4.18 DD47 pKa = 2.73 AKK49 pKa = 10.24 IYY51 pKa = 9.99 PYY53 pKa = 11.33 AFLIEE58 pKa = 4.24 DD59 pKa = 4.28 SILIAMTEE67 pKa = 3.91 EE68 pKa = 4.47 DD69 pKa = 5.42 DD70 pKa = 4.4 NTNEE74 pKa = 4.2 CC75 pKa = 4.51
Molecular weight: 8.79 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.783
IPC_protein 3.694
Toseland 3.516
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.834
Wikipedia 3.579
Rodwell 3.528
Grimsley 3.427
Solomon 3.643
Lehninger 3.605
Nozaki 3.795
DTASelect 3.935
Thurlkill 3.567
EMBOSS 3.592
Sillero 3.808
Patrickios 1.825
IPC_peptide 3.643
IPC2_peptide 3.795
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|A0A7G3MKW0|A0A7G3MKW0_9CAUD Uncharacterized protein OS=Streptococcus phage phi-SsuHCJ31_comEC OX=2664398 PE=4 SV=1
MM1 pKa = 7.72 MYY3 pKa = 10.16 TILTCTIMGLWVLIGLYY20 pKa = 9.77 FGYY23 pKa = 8.1 MTIRR27 pKa = 11.84 DD28 pKa = 4.34 DD29 pKa = 3.23 IRR31 pKa = 11.84 NEE33 pKa = 3.63 IEE35 pKa = 4.98 RR36 pKa = 11.84 KK37 pKa = 9.56 AKK39 pKa = 9.28 QNKK42 pKa = 8.32 EE43 pKa = 4.09 KK44 pKa = 10.83 LSQTPLSRR52 pKa = 11.84 KK53 pKa = 9.46 NKK55 pKa = 9.68
Molecular weight: 6.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.062
IPC2_protein 9.165
IPC_protein 9.165
Toseland 9.838
ProMoST 9.663
Dawson 10.058
Bjellqvist 9.736
Wikipedia 10.218
Rodwell 10.467
Grimsley 10.116
Solomon 10.101
Lehninger 10.087
Nozaki 9.867
DTASelect 9.706
Thurlkill 9.897
EMBOSS 10.233
Sillero 9.97
Patrickios 10.204
IPC_peptide 10.101
IPC2_peptide 8.39
IPC2.peptide.svr19 8.063
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
64
0
64
18049
42
1515
282.0
31.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.427 ± 0.41
0.914 ± 0.113
5.768 ± 0.235
7.114 ± 0.276
4.05 ± 0.269
6.405 ± 0.256
1.884 ± 0.107
6.837 ± 0.265
7.136 ± 0.249
9.402 ± 0.364
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.255 ± 0.148
4.77 ± 0.21
3.164 ± 0.157
4.338 ± 0.202
4.455 ± 0.205
6.937 ± 0.281
6.388 ± 0.402
6.66 ± 0.194
1.346 ± 0.077
3.751 ± 0.22
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here