Microlunatus soli

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Propionibacteriales; Propionibacteriaceae; Microlunatus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6014 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1P7K5|A0A1H1P7K5_9ACTN Short-chain dehydrogenase OS=Microlunatus soli OX=630515 GN=SAMN04489812_0796 PE=4 SV=1
MM1 pKa = 7.35SAASSEE7 pKa = 4.33SGSFRR12 pKa = 11.84RR13 pKa = 11.84VCPLADD19 pKa = 3.91LAAEE23 pKa = 4.03TAAAVEE29 pKa = 4.14VDD31 pKa = 3.9GEE33 pKa = 4.65DD34 pKa = 3.19IAIVNSAGEE43 pKa = 4.05IYY45 pKa = 10.49AIADD49 pKa = 3.98EE50 pKa = 5.02CSHH53 pKa = 6.55AAIPLSEE60 pKa = 4.63GDD62 pKa = 3.68VGDD65 pKa = 5.09GEE67 pKa = 4.71IEE69 pKa = 4.53CYY71 pKa = 8.43MHH73 pKa = 7.64GSRR76 pKa = 11.84FSLKK80 pKa = 9.41TGEE83 pKa = 4.23PLGLPATEE91 pKa = 4.28PVAVYY96 pKa = 8.93PVKK99 pKa = 11.06VEE101 pKa = 4.19DD102 pKa = 3.8GEE104 pKa = 4.59IYY106 pKa = 10.91VDD108 pKa = 3.9VDD110 pKa = 3.27HH111 pKa = 7.33ALNDD115 pKa = 3.52VV116 pKa = 3.39

Molecular weight: 12.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1US94|A0A1H1US94_9ACTN Isoleucine--tRNA ligase OS=Microlunatus soli OX=630515 GN=ileS PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84THH17 pKa = 6.24GFRR20 pKa = 11.84HH21 pKa = 6.29RR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AVLTARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.51GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6014

0

6014

2026290

29

3115

336.9

36.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.321 ± 0.035

0.7 ± 0.008

6.781 ± 0.03

4.948 ± 0.035

2.942 ± 0.02

9.094 ± 0.034

2.09 ± 0.014

4.727 ± 0.025

2.015 ± 0.027

10.074 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.842 ± 0.013

2.022 ± 0.017

5.711 ± 0.025

3.325 ± 0.019

7.672 ± 0.042

5.743 ± 0.025

6.155 ± 0.032

8.092 ± 0.033

1.589 ± 0.014

2.158 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski