Giant panda circovirus 4
Average proteome isoelectric point is 7.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A220IGT3|A0A220IGT3_9CIRC Capsid protein OS=Giant panda circovirus 4 OX=2016459 PE=4 SV=1
MM1 pKa = 7.14 ITPLILQINTMSRR14 pKa = 11.84 LRR16 pKa = 11.84 NCCFTINNIDD26 pKa = 3.58 ANNNIEE32 pKa = 4.22 NYY34 pKa = 10.25 FNFPIDD40 pKa = 3.49 GVKK43 pKa = 9.87 YY44 pKa = 10.5 CIYY47 pKa = 10.37 QKK49 pKa = 11.05 EE50 pKa = 4.57 KK51 pKa = 9.26 VTRR54 pKa = 11.84 DD55 pKa = 3.14 HH56 pKa = 6.06 LQGYY60 pKa = 9.6 IEE62 pKa = 4.59 FEE64 pKa = 4.17 APKK67 pKa = 10.35 SQAQIKK73 pKa = 10.09 DD74 pKa = 3.38 ILGKK78 pKa = 9.57 EE79 pKa = 3.58 AHH81 pKa = 5.86 IEE83 pKa = 4.01 KK84 pKa = 10.49 RR85 pKa = 11.84 RR86 pKa = 11.84 GTALQAQQYY95 pKa = 9.58 CMKK98 pKa = 10.19 NDD100 pKa = 3.33 SRR102 pKa = 11.84 VSGPYY107 pKa = 8.2 EE108 pKa = 3.81 FGVISHH114 pKa = 5.72 QGKK117 pKa = 10.18 RR118 pKa = 11.84 SDD120 pKa = 3.76 LKK122 pKa = 10.95 EE123 pKa = 3.51 IADD126 pKa = 3.97 RR127 pKa = 11.84 FRR129 pKa = 11.84 SGEE132 pKa = 4.21 SIKK135 pKa = 10.79 DD136 pKa = 3.33 IIDD139 pKa = 3.69 GNEE142 pKa = 4.01 TQFVRR147 pKa = 11.84 YY148 pKa = 9.69 GRR150 pKa = 11.84 GLRR153 pKa = 11.84 EE154 pKa = 4.14 LQALLQPRR162 pKa = 11.84 PRR164 pKa = 11.84 SYY166 pKa = 9.82 TKK168 pKa = 10.71 KK169 pKa = 9.85 EE170 pKa = 4.0 VVVLWGPPGVGKK182 pKa = 10.6 SYY184 pKa = 10.4 FVNHH188 pKa = 5.84 IMEE191 pKa = 4.99 LRR193 pKa = 11.84 QKK195 pKa = 10.65 LLYY198 pKa = 10.02 IKK200 pKa = 10.56 SPNTKK205 pKa = 8.41 WWDD208 pKa = 3.46 NYY210 pKa = 10.48 QGEE213 pKa = 4.55 SDD215 pKa = 3.52 ILLDD219 pKa = 4.0 EE220 pKa = 5.2 FPGTMTARR228 pKa = 11.84 DD229 pKa = 3.47 AKK231 pKa = 10.61 IILGEE236 pKa = 4.15 VNGPLEE242 pKa = 4.34 TKK244 pKa = 10.39 GGSFTTDD251 pKa = 2.04 KK252 pKa = 10.86 MEE254 pKa = 4.55 RR255 pKa = 11.84 FWITSNYY262 pKa = 8.98 EE263 pKa = 3.38 PGMWFEE269 pKa = 4.0 NAKK272 pKa = 10.82 DD273 pKa = 3.43 IDD275 pKa = 3.7 RR276 pKa = 11.84 SAVQRR281 pKa = 11.84 EE282 pKa = 4.33 VNGPLEE288 pKa = 4.35 TKK290 pKa = 10.39 GGSFTTDD297 pKa = 2.04 KK298 pKa = 10.86 MEE300 pKa = 4.55 RR301 pKa = 11.84 FWITSNYY308 pKa = 8.98 EE309 pKa = 3.38 PGMWFEE315 pKa = 4.0 NAKK318 pKa = 10.82 DD319 pKa = 3.43 IDD321 pKa = 3.7 RR322 pKa = 11.84 SAVQRR327 pKa = 11.84 RR328 pKa = 11.84 LTTICHH334 pKa = 3.83 VTEE337 pKa = 4.19 WQDD340 pKa = 3.22 TAEE343 pKa = 3.98 IFYY346 pKa = 10.81 KK347 pKa = 10.2 FFPPPSIQFGPPYY360 pKa = 10.6 DD361 pKa = 3.8 EE362 pKa = 4.11 TQNIYY367 pKa = 10.43 KK368 pKa = 10.51
Molecular weight: 42.72 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.403
IPC2_protein 6.478
IPC_protein 6.649
Toseland 6.62
ProMoST 7.161
Dawson 7.117
Bjellqvist 6.971
Wikipedia 7.088
Rodwell 7.102
Grimsley 6.707
Solomon 7.161
Lehninger 7.176
Nozaki 7.293
DTASelect 7.38
Thurlkill 7.41
EMBOSS 7.454
Sillero 7.497
Patrickios 4.342
IPC_peptide 7.161
IPC2_peptide 6.883
IPC2.peptide.svr19 6.899
Protein with the highest isoelectric point:
>tr|A0A220IGT3|A0A220IGT3_9CIRC Capsid protein OS=Giant panda circovirus 4 OX=2016459 PE=4 SV=1
MM1 pKa = 7.87 RR2 pKa = 11.84 KK3 pKa = 9.42 FFINFFLPLRR13 pKa = 11.84 YY14 pKa = 9.9 NLDD17 pKa = 3.03 HH18 pKa = 8.08 RR19 pKa = 11.84 MMKK22 pKa = 10.27 RR23 pKa = 11.84 KK24 pKa = 8.16 TYY26 pKa = 7.83 TNRR29 pKa = 11.84 SYY31 pKa = 10.2 RR32 pKa = 11.84 TTYY35 pKa = 10.13 KK36 pKa = 9.72 RR37 pKa = 11.84 RR38 pKa = 11.84 KK39 pKa = 8.25 YY40 pKa = 8.38 GTNRR44 pKa = 11.84 KK45 pKa = 8.6 VPKK48 pKa = 9.5 PLRR51 pKa = 11.84 TAIKK55 pKa = 9.18 KK56 pKa = 7.99 TMNRR60 pKa = 11.84 QLEE63 pKa = 4.48 TKK65 pKa = 9.84 KK66 pKa = 10.56 CYY68 pKa = 8.16 QQGMTVMQANFQANTMYY85 pKa = 10.51 TDD87 pKa = 4.2 NILYY91 pKa = 9.19 NIPQNATEE99 pKa = 4.53 GGRR102 pKa = 11.84 IGDD105 pKa = 4.7 RR106 pKa = 11.84 INISKK111 pKa = 10.21 VVISGEE117 pKa = 4.34 FQCQPTDD124 pKa = 3.16 NVFWHH129 pKa = 6.72 LYY131 pKa = 8.05 IFRR134 pKa = 11.84 SALYY138 pKa = 10.02 NDD140 pKa = 3.71 NTTPPLYY147 pKa = 10.88 GIMPYY152 pKa = 10.59 SRR154 pKa = 11.84 FYY156 pKa = 10.98 HH157 pKa = 5.51 PVGSIVNPNVDD168 pKa = 5.15 LPDD171 pKa = 4.91 PDD173 pKa = 3.11 QVRR176 pKa = 11.84 ILKK179 pKa = 9.48 YY180 pKa = 10.21 KK181 pKa = 10.38 RR182 pKa = 11.84 IRR184 pKa = 11.84 VDD186 pKa = 3.05 PANNGGICIRR196 pKa = 11.84 RR197 pKa = 11.84 FYY199 pKa = 11.19 LSVNMKK205 pKa = 9.76 NAAYY209 pKa = 9.92 QYY211 pKa = 9.34 ATDD214 pKa = 4.04 GSSYY218 pKa = 9.73 GRR220 pKa = 11.84 NYY222 pKa = 10.74 NLYY225 pKa = 10.11 FGVICRR231 pKa = 11.84 GQEE234 pKa = 3.94 GNQLNITEE242 pKa = 3.89 QRR244 pKa = 11.84 IFYY247 pKa = 10.52 KK248 pKa = 10.75 DD249 pKa = 2.89 AA250 pKa = 5.33
Molecular weight: 29.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.688
IPC2_protein 9.736
IPC_protein 10.087
Toseland 9.926
ProMoST 9.809
Dawson 10.233
Bjellqvist 9.984
Wikipedia 10.467
Rodwell 10.452
Grimsley 10.335
Solomon 10.248
Lehninger 10.189
Nozaki 9.955
DTASelect 9.97
Thurlkill 10.043
EMBOSS 10.365
Sillero 10.145
Patrickios 5.08
IPC_peptide 10.248
IPC2_peptide 8.902
IPC2.peptide.svr19 8.62
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
618
250
368
309.0
36.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.045 ± 0.026
1.456 ± 0.084
5.178 ± 0.455
5.34 ± 1.952
5.016 ± 0.126
6.634 ± 0.371
1.294 ± 0.055
7.767 ± 0.331
6.796 ± 0.232
5.663 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.913 ± 0.402
7.282 ± 1.355
5.178 ± 0.013
5.178 ± 0.013
7.443 ± 1.027
4.531 ± 0.544
6.472 ± 0.191
4.369 ± 0.252
1.456 ± 0.618
5.987 ± 1.411
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here