Hubei tombus-like virus 21

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGC9|A0A1L3KGC9_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 21 OX=1923268 PE=4 SV=1
MM1 pKa = 6.85NTVKK5 pKa = 10.74GSTFQIMTTGAKK17 pKa = 9.16PCRR20 pKa = 11.84HH21 pKa = 4.87TRR23 pKa = 11.84MLTRR27 pKa = 11.84HH28 pKa = 4.79YY29 pKa = 10.04HH30 pKa = 4.73GHH32 pKa = 6.89PLMEE36 pKa = 4.17QQHH39 pKa = 6.22IFQSCSSNEE48 pKa = 3.63YY49 pKa = 9.51RR50 pKa = 11.84GLKK53 pKa = 8.26EE54 pKa = 3.58RR55 pKa = 11.84HH56 pKa = 6.09VIEE59 pKa = 4.71QLGCSKK65 pKa = 11.15DD66 pKa = 3.78FVKK69 pKa = 10.15WAHH72 pKa = 5.41QFIAKK77 pKa = 9.89LDD79 pKa = 3.89LPSIEE84 pKa = 4.32PMSDD88 pKa = 3.03EE89 pKa = 4.28EE90 pKa = 5.15LIASRR95 pKa = 11.84PPRR98 pKa = 11.84MKK100 pKa = 10.17KK101 pKa = 10.09RR102 pKa = 11.84YY103 pKa = 9.49ASGLKK108 pKa = 10.16VPFEE112 pKa = 4.49GKK114 pKa = 8.08IHH116 pKa = 6.5ARR118 pKa = 11.84VSCFIKK124 pKa = 10.35NEE126 pKa = 3.51KK127 pKa = 8.66MAIEE131 pKa = 4.23KK132 pKa = 9.25PKK134 pKa = 9.92PRR136 pKa = 11.84CINYY140 pKa = 9.64RR141 pKa = 11.84NSIFTAQLAKK151 pKa = 8.54WTVPIEE157 pKa = 3.95KK158 pKa = 10.33LLAHH162 pKa = 6.98WPLPDD167 pKa = 3.56NGGVPFMSKK176 pKa = 9.64GRR178 pKa = 11.84NAMDD182 pKa = 4.77LGQMLNQAYY191 pKa = 7.9TLAGKK196 pKa = 10.31KK197 pKa = 9.78YY198 pKa = 9.26IHH200 pKa = 6.63LVDD203 pKa = 4.14HH204 pKa = 6.6SAYY207 pKa = 10.26DD208 pKa = 3.41GSINVEE214 pKa = 4.16HH215 pKa = 7.21IKK217 pKa = 10.88LEE219 pKa = 4.24RR220 pKa = 11.84KK221 pKa = 8.81WYY223 pKa = 10.79QKK225 pKa = 9.94MSQNDD230 pKa = 3.19SSLYY234 pKa = 10.84DD235 pKa = 4.78LITLQLEE242 pKa = 4.23NKK244 pKa = 8.61IVSRR248 pKa = 11.84NGVKK252 pKa = 10.3AKK254 pKa = 10.51CKK256 pKa = 8.78GVRR259 pKa = 11.84MSGDD263 pKa = 3.45ANTSLGNSVINYY275 pKa = 9.47IMLRR279 pKa = 11.84YY280 pKa = 9.42QYY282 pKa = 9.67PDD284 pKa = 3.15SIIIVNGDD292 pKa = 3.4DD293 pKa = 3.84SVIFSDD299 pKa = 4.54LVEE302 pKa = 4.75PAHH305 pKa = 6.12SWEE308 pKa = 4.17EE309 pKa = 3.73VGVNSKK315 pKa = 10.63VSVVQEE321 pKa = 4.14FTDD324 pKa = 4.83LEE326 pKa = 4.62YY327 pKa = 10.92CQSRR331 pKa = 11.84PVHH334 pKa = 5.47TEE336 pKa = 3.38KK337 pKa = 10.59GWVMMRR343 pKa = 11.84DD344 pKa = 4.44PIRR347 pKa = 11.84SLSRR351 pKa = 11.84MAYY354 pKa = 10.03RR355 pKa = 11.84LTHH358 pKa = 6.43GKK360 pKa = 10.38DD361 pKa = 3.13SDD363 pKa = 3.19WFYY366 pKa = 10.88TLGVGEE372 pKa = 4.3LHH374 pKa = 7.02SNPFDD379 pKa = 4.07PFMQAMAEE387 pKa = 4.14GFIKK391 pKa = 10.36RR392 pKa = 11.84GKK394 pKa = 8.68GGKK397 pKa = 8.84FRR399 pKa = 11.84SHH401 pKa = 5.56LRR403 pKa = 11.84EE404 pKa = 3.66YY405 pKa = 10.25RR406 pKa = 11.84HH407 pKa = 5.76AVGWTKK413 pKa = 10.65DD414 pKa = 3.46VEE416 pKa = 4.44RR417 pKa = 11.84ATHH420 pKa = 6.05YY421 pKa = 10.34STINWQTTFNIDD433 pKa = 3.17SAMMKK438 pKa = 10.6SMVDD442 pKa = 3.11MVLRR446 pKa = 11.84QCCYY450 pKa = 11.07ASTTSNYY457 pKa = 9.75CAA459 pKa = 4.4

Molecular weight: 52.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGC9|A0A1L3KGC9_9VIRU RNA-directed RNA polymerase OS=Hubei tombus-like virus 21 OX=1923268 PE=4 SV=1
MM1 pKa = 7.39SNVGNRR7 pKa = 11.84LKK9 pKa = 10.4GTAHH13 pKa = 6.33NCASCHH19 pKa = 5.27RR20 pKa = 11.84RR21 pKa = 11.84LRR23 pKa = 11.84GQPGQRR29 pKa = 11.84FCVIVTHH36 pKa = 6.97NVDD39 pKa = 3.13QQLRR43 pKa = 11.84NLAAKK48 pKa = 9.89SWVFPNEE55 pKa = 4.0CPKK58 pKa = 10.89CGEE61 pKa = 4.18AKK63 pKa = 10.21YY64 pKa = 10.18HH65 pKa = 5.93HH66 pKa = 6.32QLANGKK72 pKa = 7.29EE73 pKa = 3.89VRR75 pKa = 11.84QCKK78 pKa = 6.85TCRR81 pKa = 11.84HH82 pKa = 5.08KK83 pKa = 10.13WGKK86 pKa = 9.32ILRR89 pKa = 11.84SSLPAKK95 pKa = 10.58SPVRR99 pKa = 11.84QPAAAPQKK107 pKa = 10.6RR108 pKa = 11.84PLCNTCMQPGMLVFGDD124 pKa = 4.44LGHH127 pKa = 7.24CLRR130 pKa = 11.84CGHH133 pKa = 5.86FTDD136 pKa = 5.36LLTLGTCSSKK146 pKa = 10.91QKK148 pKa = 10.88AKK150 pKa = 9.47AAKK153 pKa = 9.06VVKK156 pKa = 10.4HH157 pKa = 5.8NLAAPKK163 pKa = 9.1MAKK166 pKa = 8.88QAYY169 pKa = 8.19ARR171 pKa = 11.84AVEE174 pKa = 4.42EE175 pKa = 4.03RR176 pKa = 11.84LQSKK180 pKa = 8.66SFKK183 pKa = 10.52KK184 pKa = 10.12PLPTPRR190 pKa = 11.84SIFNAAKK197 pKa = 9.34PIPMPRR203 pKa = 11.84ARR205 pKa = 11.84PGPSKK210 pKa = 10.86AVVSKK215 pKa = 9.38KK216 pKa = 7.95TQVKK220 pKa = 9.48PKK222 pKa = 9.9LATIQEE228 pKa = 4.22HH229 pKa = 6.5RR230 pKa = 11.84KK231 pKa = 9.8LSPPANTKK239 pKa = 10.61KK240 pKa = 10.61LVNQQLAGTFGQPQPRR256 pKa = 11.84KK257 pKa = 10.03SRR259 pKa = 11.84DD260 pKa = 3.31SLAPRR265 pKa = 11.84ANKK268 pKa = 9.83RR269 pKa = 11.84VIRR272 pKa = 11.84AIVEE276 pKa = 4.32DD277 pKa = 4.04NNGQSDD283 pKa = 4.21VLAMRR288 pKa = 11.84KK289 pKa = 9.46AYY291 pKa = 10.17NSPQVSGNPPTKK303 pKa = 9.87EE304 pKa = 3.45DD305 pKa = 3.37FRR307 pKa = 11.84FVRR310 pKa = 11.84NLRR313 pKa = 11.84LSPHH317 pKa = 6.85LYY319 pKa = 10.15IPGEE323 pKa = 4.05GAGVWVEE330 pKa = 4.46VPALMKK336 pKa = 10.67NPRR339 pKa = 11.84LEE341 pKa = 4.22YY342 pKa = 10.9LDD344 pKa = 4.18IAWLAAMNKK353 pKa = 7.31TQEE356 pKa = 3.78QRR358 pKa = 11.84LRR360 pKa = 11.84LALILGKK367 pKa = 9.88IANQQRR373 pKa = 11.84LNKK376 pKa = 10.0AMRR379 pKa = 11.84KK380 pKa = 7.26TSPTKK385 pKa = 10.36KK386 pKa = 9.9QQ387 pKa = 2.99

Molecular weight: 43.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1218

372

459

406.0

45.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.553 ± 1.15

1.888 ± 0.632

3.53 ± 0.678

4.105 ± 0.553

3.12 ± 0.311

6.076 ± 0.284

3.366 ± 0.367

4.68 ± 0.761

7.225 ± 1.439

7.553 ± 0.592

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.12 ± 0.795

4.926 ± 0.297

6.24 ± 1.054

4.68 ± 0.696

6.568 ± 0.664

7.553 ± 0.833

5.993 ± 1.277

7.143 ± 0.947

1.724 ± 0.271

2.956 ± 0.707
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski