Thermostaphylospora chromogena

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Thermostaphylospora

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5075 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1F6K4|A0A1H1F6K4_9ACTN D-serine deaminase pyridoxal phosphate-dependent OS=Thermostaphylospora chromogena OX=35622 GN=SAMN04489764_2811 PE=4 SV=1
MM1 pKa = 7.77SIPEE5 pKa = 3.89EE6 pKa = 3.56LRR8 pKa = 11.84YY9 pKa = 8.56TKK11 pKa = 9.54EE12 pKa = 4.19HH13 pKa = 6.19EE14 pKa = 4.36WVAGLDD20 pKa = 3.62DD21 pKa = 4.58GATVTVGITAYY32 pKa = 10.31AAEE35 pKa = 4.0QLGDD39 pKa = 3.68VVYY42 pKa = 10.24VQPPEE47 pKa = 4.0VGSVITAGDD56 pKa = 3.55SVGEE60 pKa = 4.15VEE62 pKa = 4.51STKK65 pKa = 10.71SVSDD69 pKa = 3.26IYY71 pKa = 11.4APVSGEE77 pKa = 3.99VVEE80 pKa = 4.84VNQAVVDD87 pKa = 4.33DD88 pKa = 4.51PSLVNSDD95 pKa = 4.02PYY97 pKa = 11.4GEE99 pKa = 4.16GWLFKK104 pKa = 10.77VHH106 pKa = 6.37VDD108 pKa = 4.76DD109 pKa = 6.03VPADD113 pKa = 3.98LLTADD118 pKa = 3.99QYY120 pKa = 12.29APLTSGEE127 pKa = 4.3SS128 pKa = 3.31

Molecular weight: 13.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H1DGK2|A0A1H1DGK2_9ACTN Uroporphyrinogen-III synthase /uroporphyrinogen-III C-methyltransferase OS=Thermostaphylospora chromogena OX=35622 GN=SAMN04489764_1996 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.55LLKK22 pKa = 8.44KK23 pKa = 9.17TRR25 pKa = 11.84IQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.73KK32 pKa = 9.84

Molecular weight: 4.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5075

0

5075

1694156

30

5338

333.8

35.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.3 ± 0.05

0.785 ± 0.01

5.755 ± 0.025

6.101 ± 0.034

2.754 ± 0.021

9.18 ± 0.03

2.126 ± 0.016

3.787 ± 0.026

1.998 ± 0.027

10.292 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.901 ± 0.014

1.725 ± 0.017

6.282 ± 0.038

2.384 ± 0.022

8.83 ± 0.039

4.807 ± 0.022

5.63 ± 0.022

8.762 ± 0.029

1.494 ± 0.014

2.108 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski