Kirkovirus Equ1

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4AQY1|A0A0H4AQY1_9VIRU Uncharacterized protein OS=Kirkovirus Equ1 OX=1673637 PE=4 SV=1
MM1 pKa = 7.3YY2 pKa = 10.59NDD4 pKa = 5.41LYY6 pKa = 11.72DD7 pKa = 3.7MDD9 pKa = 5.14PFNEE13 pKa = 3.97LAYY16 pKa = 10.7DD17 pKa = 4.11PDD19 pKa = 3.65QFGIDD24 pKa = 3.37DD25 pKa = 4.73KK26 pKa = 12.08YY27 pKa = 11.19NFTYY31 pKa = 9.66EE32 pKa = 4.08GKK34 pKa = 10.38IIDD37 pKa = 3.98VPSDD41 pKa = 3.21VSSQVQQDD49 pKa = 3.91YY50 pKa = 11.82VNGKK54 pKa = 9.68DD55 pKa = 3.56PQHH58 pKa = 6.07SLKK61 pKa = 10.96SLMEE65 pKa = 3.94QYY67 pKa = 10.51LQEE70 pKa = 4.59HH71 pKa = 6.53SPQSRR76 pKa = 11.84YY77 pKa = 9.2EE78 pKa = 4.08MIKK81 pKa = 10.19EE82 pKa = 3.89EE83 pKa = 4.48PNRR86 pKa = 11.84EE87 pKa = 3.51IYY89 pKa = 10.45EE90 pKa = 4.18NYY92 pKa = 9.75EE93 pKa = 3.71FWHH96 pKa = 6.66DD97 pKa = 3.77VVPFKK102 pKa = 10.99AFKK105 pKa = 10.61KK106 pKa = 9.24NEE108 pKa = 3.75QMTTLSNLAGWVVIPARR125 pKa = 11.84NVGVLRR131 pKa = 11.84NDD133 pKa = 3.45TRR135 pKa = 11.84RR136 pKa = 11.84KK137 pKa = 6.23PTPLIYY143 pKa = 10.45LPHH146 pKa = 6.52EE147 pKa = 4.3AMSNFDD153 pKa = 3.11KK154 pKa = 11.12HH155 pKa = 6.53FNN157 pKa = 3.63

Molecular weight: 18.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4ATG1|A0A0H4ATG1_9VIRU Uncharacterized protein OS=Kirkovirus Equ1 OX=1673637 PE=4 SV=1
MM1 pKa = 7.67YY2 pKa = 10.71YY3 pKa = 9.88PDD5 pKa = 5.03PNPARR10 pKa = 11.84DD11 pKa = 4.2NHH13 pKa = 6.0PQWQSDD19 pKa = 2.77AKK21 pKa = 9.75TFFEE25 pKa = 4.15VSYY28 pKa = 11.13PEE30 pKa = 3.89VTKK33 pKa = 10.31MIEE36 pKa = 3.75VAKK39 pKa = 9.14YY40 pKa = 9.41HH41 pKa = 5.86SEE43 pKa = 3.59ARR45 pKa = 11.84EE46 pKa = 3.98AIHH49 pKa = 5.56RR50 pKa = 11.84HH51 pKa = 4.9KK52 pKa = 11.08NIMSDD57 pKa = 3.34IIFYY61 pKa = 7.28VTQWKK66 pKa = 10.06RR67 pKa = 11.84YY68 pKa = 7.22HH69 pKa = 5.57NQYY72 pKa = 10.14ARR74 pKa = 11.84YY75 pKa = 8.93VIVYY79 pKa = 7.92VKK81 pKa = 9.7FRR83 pKa = 11.84IPIRR87 pKa = 11.84YY88 pKa = 8.56IAISNMFPSARR99 pKa = 11.84VNYY102 pKa = 10.01FYY104 pKa = 11.14DD105 pKa = 4.97DD106 pKa = 3.61GDD108 pKa = 3.94AMDD111 pKa = 3.84RR112 pKa = 11.84HH113 pKa = 6.06YY114 pKa = 9.26PTWTRR119 pKa = 11.84GWTGRR124 pKa = 11.84VYY126 pKa = 10.06IYY128 pKa = 10.44RR129 pKa = 11.84RR130 pKa = 11.84NGIDD134 pKa = 3.69LDD136 pKa = 4.32VPEE139 pKa = 4.53STYY142 pKa = 10.57DD143 pKa = 3.29HH144 pKa = 7.33RR145 pKa = 11.84DD146 pKa = 3.26YY147 pKa = 11.26LPRR150 pKa = 11.84RR151 pKa = 11.84RR152 pKa = 11.84ATPEE156 pKa = 3.65SFKK159 pKa = 10.69RR160 pKa = 11.84AYY162 pKa = 10.41HH163 pKa = 6.12DD164 pKa = 4.18AKK166 pKa = 10.65FKK168 pKa = 11.24NLIRR172 pKa = 11.84KK173 pKa = 7.9NKK175 pKa = 7.35KK176 pKa = 8.81KK177 pKa = 10.53KK178 pKa = 9.41YY179 pKa = 9.66VRR181 pKa = 11.84ILNTSPMYY189 pKa = 10.22PSNHH193 pKa = 6.5KK194 pKa = 10.48YY195 pKa = 10.65YY196 pKa = 11.08NN197 pKa = 3.36

Molecular weight: 24.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1041

157

305

208.2

24.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.419 ± 0.638

0.672 ± 0.288

5.764 ± 0.837

6.34 ± 1.242

5.283 ± 0.698

4.515 ± 0.918

3.266 ± 0.495

7.205 ± 0.692

6.244 ± 0.566

5.764 ± 0.918

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.209 ± 0.568

6.724 ± 0.533

5.187 ± 0.889

3.65 ± 0.805

5.668 ± 1.188

8.261 ± 1.442

5.091 ± 0.955

5.668 ± 0.433

1.249 ± 0.418

6.82 ± 1.461
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski