Vibrio phage VP5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Enhodamvirus; Vibrio virus VP5

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6R6B3|Q6R6B3_9CAUD Peptidase_M15_3 domain-containing protein OS=Vibrio phage VP5 OX=260827 PE=4 SV=1
MM1 pKa = 7.66PSLVSIYY8 pKa = 10.77NKK10 pKa = 10.52ALAHH14 pKa = 5.83VGAKK18 pKa = 9.58PVLSEE23 pKa = 3.67TDD25 pKa = 3.1ANTRR29 pKa = 11.84AEE31 pKa = 4.37MCNAMYY37 pKa = 10.65EE38 pKa = 4.25FALRR42 pKa = 11.84SALEE46 pKa = 3.75ARR48 pKa = 11.84EE49 pKa = 3.86WSFAAMRR56 pKa = 11.84VRR58 pKa = 11.84LLPDD62 pKa = 3.81PLITPAWGYY71 pKa = 10.66NYY73 pKa = 10.45GYY75 pKa = 10.44RR76 pKa = 11.84LPNDD80 pKa = 4.68CIRR83 pKa = 11.84VCEE86 pKa = 4.05VRR88 pKa = 11.84DD89 pKa = 3.81GNSYY93 pKa = 10.75NIDD96 pKa = 3.1WAIEE100 pKa = 4.14SGFLYY105 pKa = 9.28ATRR108 pKa = 11.84PTVDD112 pKa = 2.62VKK114 pKa = 10.86YY115 pKa = 10.83NRR117 pKa = 11.84LVEE120 pKa = 4.45DD121 pKa = 4.45PNAFTPTFVEE131 pKa = 4.11ALSLKK136 pKa = 9.98LASDD140 pKa = 3.57ICIPLTEE147 pKa = 4.13NRR149 pKa = 11.84GLGNDD154 pKa = 4.46LISRR158 pKa = 11.84YY159 pKa = 10.29LMAVSEE165 pKa = 4.4GAALDD170 pKa = 3.69GLQASRR176 pKa = 11.84EE177 pKa = 3.98RR178 pKa = 11.84LRR180 pKa = 11.84ADD182 pKa = 3.3TLINARR188 pKa = 11.84RR189 pKa = 11.84GCGRR193 pKa = 11.84MDD195 pKa = 3.44RR196 pKa = 11.84PYY198 pKa = 10.28PQTGSGKK205 pKa = 10.13FYY207 pKa = 10.65DD208 pKa = 4.03IAPPTIPDD216 pKa = 3.74SPPVGNISGPSSVQLGQPFSFIAQVTGTQPISYY249 pKa = 8.76QWYY252 pKa = 8.64KK253 pKa = 11.09NGIAIPSATSVSFGVEE269 pKa = 3.66SATNNDD275 pKa = 3.14EE276 pKa = 4.88GEE278 pKa = 4.34YY279 pKa = 10.46HH280 pKa = 7.03CLFSNSAGVVISNKK294 pKa = 9.05ISLEE298 pKa = 3.94IGCFPPVHH306 pKa = 6.77NITGPTTVGEE316 pKa = 4.37GKK318 pKa = 10.04SFSMVCTVTAGNLPVTYY335 pKa = 9.76QWQRR339 pKa = 11.84DD340 pKa = 3.73GQNIAGATTATLSVAAATVVDD361 pKa = 3.99SGEE364 pKa = 4.04YY365 pKa = 10.29DD366 pKa = 3.42CLVSNQCGSNVSSTNKK382 pKa = 8.22ITLDD386 pKa = 3.79VIPLVVPTVSISPVSASPLEE406 pKa = 4.13PAPVTFTASVVDD418 pKa = 4.82DD419 pKa = 4.5GGAPPVTLKK428 pKa = 10.32WYY430 pKa = 10.86LNGNLVQNGGTTYY443 pKa = 9.97TSPPTAVGQNRR454 pKa = 11.84TVSVVGTNSVGDD466 pKa = 4.15SVPASASLTPVSNFISQSTFTTNAAFTLHH495 pKa = 7.18PDD497 pKa = 3.38CTFVQVQGCGGGGGGGSGDD516 pKa = 4.23AIGTEE521 pKa = 4.25LTGGGGGGGAALVSVLSSNAIGGQTATIVIGGGGAGAPNVGTTFGGNGGNTSVSGAGIIGLSWTGGKK588 pKa = 9.82GGRR591 pKa = 11.84SGYY594 pKa = 10.43RR595 pKa = 11.84SDD597 pKa = 4.04GSGNGAFGGAGGNNSANTGGGAGGVSSNGGNGVNGGGGGGGSNNDD642 pKa = 2.96LVLRR646 pKa = 11.84RR647 pKa = 11.84GGNGGGSNGGIGGQGVGTTDD667 pKa = 3.51GPSGGGGGNSAVWWDD682 pKa = 3.45NADD685 pKa = 3.43AGRR688 pKa = 11.84GGNGVTGNSGNQTGGHH704 pKa = 5.5ATGNGNGGGGGGGGVPDD721 pKa = 4.59FGGAGGNGSGGKK733 pKa = 10.12VIIRR737 pKa = 11.84QYY739 pKa = 10.65RR740 pKa = 11.84RR741 pKa = 11.84PII743 pKa = 3.5

Molecular weight: 74.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6R6B2|Q6R6B2_9CAUD Major tail subunit OS=Vibrio phage VP5 OX=260827 PE=4 SV=1
MM1 pKa = 7.52PRR3 pKa = 11.84KK4 pKa = 9.96CFEE7 pKa = 3.69GWKK10 pKa = 10.3EE11 pKa = 3.45EE12 pKa = 5.2DD13 pKa = 3.06IAKK16 pKa = 9.27MFGKK20 pKa = 9.09TDD22 pKa = 3.36TDD24 pKa = 4.91LITAEE29 pKa = 5.24DD30 pKa = 3.51IADD33 pKa = 4.46AIKK36 pKa = 10.59GKK38 pKa = 9.68KK39 pKa = 7.02QEE41 pKa = 4.46KK42 pKa = 8.43IAVYY46 pKa = 9.78KK47 pKa = 9.77QAEE50 pKa = 4.38AIKK53 pKa = 10.34KK54 pKa = 8.8GNEE57 pKa = 3.59VLTQSKK63 pKa = 10.83DD64 pKa = 3.3PASALLGMLSRR75 pKa = 11.84DD76 pKa = 3.39PNEE79 pKa = 3.82EE80 pKa = 4.07VKK82 pKa = 10.63FLSADD87 pKa = 3.31QRR89 pKa = 11.84INAIRR94 pKa = 11.84AVSKK98 pKa = 11.13AKK100 pKa = 10.46ISDD103 pKa = 3.84FMADD107 pKa = 4.01LAPTTRR113 pKa = 11.84QIFAGIATGEE123 pKa = 3.97RR124 pKa = 11.84RR125 pKa = 11.84LTKK128 pKa = 10.1SQQRR132 pKa = 11.84LLDD135 pKa = 4.09DD136 pKa = 4.96FVHH139 pKa = 6.27EE140 pKa = 4.37LYY142 pKa = 10.86GRR144 pKa = 11.84QTGNADD150 pKa = 3.31ALKK153 pKa = 10.39AAKK156 pKa = 9.05GWKK159 pKa = 9.5KK160 pKa = 9.59ATEE163 pKa = 4.15DD164 pKa = 3.23LNARR168 pKa = 11.84FGQAGGHH175 pKa = 4.99MAEE178 pKa = 4.55LDD180 pKa = 3.72DD181 pKa = 3.55WRR183 pKa = 11.84LPQKK187 pKa = 9.96HH188 pKa = 5.55NRR190 pKa = 11.84MAISKK195 pKa = 10.02AGADD199 pKa = 3.36VWVEE203 pKa = 4.13KK204 pKa = 10.91VWDD207 pKa = 4.76LIDD210 pKa = 4.04RR211 pKa = 11.84DD212 pKa = 3.91KK213 pKa = 10.96MVKK216 pKa = 10.02KK217 pKa = 10.2LRR219 pKa = 11.84KK220 pKa = 10.13GKK222 pKa = 10.38DD223 pKa = 3.07EE224 pKa = 4.88DD225 pKa = 3.79NLRR228 pKa = 11.84EE229 pKa = 4.07ALYY232 pKa = 10.62SVYY235 pKa = 11.17NNIVTDD241 pKa = 4.19GMSSSKK247 pKa = 9.54TLSKK251 pKa = 10.85KK252 pKa = 8.77FTDD255 pKa = 3.26MMRR258 pKa = 11.84SEE260 pKa = 4.39RR261 pKa = 11.84FITFKK266 pKa = 11.19DD267 pKa = 3.09SDD269 pKa = 3.55SWLKK273 pKa = 8.89YY274 pKa = 8.94QRR276 pKa = 11.84EE277 pKa = 4.12FGDD280 pKa = 3.78TNVYY284 pKa = 11.19ASMLGHH290 pKa = 7.29IDD292 pKa = 3.3NMSRR296 pKa = 11.84AIGMMEE302 pKa = 4.21TFGPDD307 pKa = 3.13PDD309 pKa = 3.64IGFNTLEE316 pKa = 4.17RR317 pKa = 11.84AVKK320 pKa = 8.58TKK322 pKa = 10.6KK323 pKa = 10.59GLTSRR328 pKa = 11.84QPTGARR334 pKa = 11.84PTFDD338 pKa = 2.99MLMGYY343 pKa = 10.95NMVEE347 pKa = 4.19EE348 pKa = 4.03QTVWGNRR355 pKa = 11.84VAGLRR360 pKa = 11.84NLWTASKK367 pKa = 10.51LGAAVVSALTDD378 pKa = 3.34SVYY381 pKa = 11.46ASMAASYY388 pKa = 11.13NAMSPARR395 pKa = 11.84VLRR398 pKa = 11.84RR399 pKa = 11.84MLSEE403 pKa = 3.8VMKK406 pKa = 9.81PSKK409 pKa = 10.78SEE411 pKa = 3.66ASRR414 pKa = 11.84KK415 pKa = 9.07LWAQDD420 pKa = 3.33FGFGAEE426 pKa = 4.04FALDD430 pKa = 3.77RR431 pKa = 11.84MAMTSDD437 pKa = 3.49YY438 pKa = 8.57TQSFGGHH445 pKa = 5.76RR446 pKa = 11.84SRR448 pKa = 11.84NLAEE452 pKa = 3.71AVMVVSGMNQWTQSARR468 pKa = 11.84ASFQFEE474 pKa = 4.0FATALTRR481 pKa = 11.84AADD484 pKa = 4.22SKK486 pKa = 11.01WSDD489 pKa = 3.69LPEE492 pKa = 4.25KK493 pKa = 9.71MRR495 pKa = 11.84NSMGRR500 pKa = 11.84YY501 pKa = 9.46GITEE505 pKa = 4.2SDD507 pKa = 3.06WAAIAAAPRR516 pKa = 11.84TNYY519 pKa = 9.85KK520 pKa = 8.69GNKK523 pKa = 8.84MIDD526 pKa = 3.95PRR528 pKa = 11.84NMDD531 pKa = 4.11AEE533 pKa = 4.43LQTKK537 pKa = 9.25LVGMVDD543 pKa = 4.38GEE545 pKa = 4.57TMMAVPTPDD554 pKa = 2.75ARR556 pKa = 11.84TRR558 pKa = 11.84AFMAGGTKK566 pKa = 10.05SGNFGGEE573 pKa = 3.73LHH575 pKa = 6.91RR576 pKa = 11.84SLFMFHH582 pKa = 7.09SFPITTIMNQWRR594 pKa = 11.84RR595 pKa = 11.84VFTGKK600 pKa = 10.49GYY602 pKa = 10.71SGAFDD607 pKa = 3.68RR608 pKa = 11.84MSAAAIMVGATSVLGVGIIQAKK630 pKa = 10.37DD631 pKa = 2.95ILNGKK636 pKa = 8.84KK637 pKa = 9.12PRR639 pKa = 11.84SMSDD643 pKa = 2.81PKK645 pKa = 11.07LWIEE649 pKa = 4.16GMAQGGSFNYY659 pKa = 10.07IGDD662 pKa = 4.36LMRR665 pKa = 11.84NAASGYY671 pKa = 7.93SHH673 pKa = 7.71DD674 pKa = 3.7MTSYY678 pKa = 10.6VGGPVLAYY686 pKa = 10.14GDD688 pKa = 4.21WVAMTAADD696 pKa = 3.96MAKK699 pKa = 10.46GDD701 pKa = 4.53AEE703 pKa = 4.16SAMARR708 pKa = 11.84TANFATQQIPFNNLWYY724 pKa = 9.66TKK726 pKa = 10.08IATDD730 pKa = 3.74RR731 pKa = 11.84LLMDD735 pKa = 5.18RR736 pKa = 11.84IRR738 pKa = 11.84RR739 pKa = 11.84LSDD742 pKa = 3.08PEE744 pKa = 3.89YY745 pKa = 11.05DD746 pKa = 3.37KK747 pKa = 11.59KK748 pKa = 11.03QLNKK752 pKa = 8.09MRR754 pKa = 11.84KK755 pKa = 5.49MQRR758 pKa = 11.84TSQQEE763 pKa = 4.44YY764 pKa = 7.04WWSPPIGGQSNIEE777 pKa = 4.28SPFEE781 pKa = 3.78EE782 pKa = 4.53

Molecular weight: 87.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

5479

105

782

456.6

50.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.414 ± 0.54

1.04 ± 0.182

6.516 ± 0.385

6.352 ± 0.674

3.687 ± 0.285

9.034 ± 1.332

1.825 ± 0.29

5.676 ± 0.368

5.84 ± 0.619

6.881 ± 0.409

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.34 ± 0.508

4.636 ± 0.396

4.07 ± 0.259

3.815 ± 0.27

6.023 ± 0.356

6.059 ± 0.572

5.384 ± 0.431

5.986 ± 0.428

2.117 ± 0.251

3.304 ± 0.276
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski