Desulfosporosinus youngiae DSM 17734
Average proteome isoelectric point is 6.39
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5108 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|H5Y5T4|H5Y5T4_9FIRM Uncharacterized protein OS=Desulfosporosinus youngiae DSM 17734 OX=768710 GN=DesyoDRAFT_3826 PE=4 SV=1
MM1 pKa = 7.14 KK2 pKa = 9.47 TKK4 pKa = 10.55 QKK6 pKa = 10.29 ILSLFMMLILGLTFFVAPAPAKK28 pKa = 10.38 AATDD32 pKa = 3.76 MLTLADD38 pKa = 4.39 SEE40 pKa = 4.83 HH41 pKa = 5.65 FTILGEE47 pKa = 4.4 VNDD50 pKa = 4.53 EE51 pKa = 4.17 VADD54 pKa = 3.88 IQVLGLDD61 pKa = 3.49 GTTWMTEE68 pKa = 4.04 EE69 pKa = 4.42 ISDD72 pKa = 4.21 PEE74 pKa = 4.34 NIEE77 pKa = 3.82 WTTSDD82 pKa = 3.03 ASVVKK87 pKa = 10.09 FLDD90 pKa = 3.68 GSTPVATIDD99 pKa = 3.28 EE100 pKa = 4.56 TDD102 pKa = 3.31 TVKK105 pKa = 10.28 IRR107 pKa = 11.84 LLAEE111 pKa = 3.54 GRR113 pKa = 11.84 AYY115 pKa = 9.02 VTAHH119 pKa = 6.17 YY120 pKa = 11.15 DD121 pKa = 3.35 SMEE124 pKa = 3.73 VSAYY128 pKa = 10.48 VVVEE132 pKa = 4.17 TKK134 pKa = 10.65 GLPTASVSDD143 pKa = 2.99 ISVYY147 pKa = 10.62 VDD149 pKa = 3.72 APGTVNDD156 pKa = 4.85 FYY158 pKa = 11.69 VINEE162 pKa = 4.28 TVNLSDD168 pKa = 6.05 LDD170 pKa = 3.74 WLLDD174 pKa = 3.67 QSQTLQLNCSALHH187 pKa = 5.92 ALAMAGNEE195 pKa = 4.32 YY196 pKa = 10.96 YY197 pKa = 10.62 SDD199 pKa = 4.51 PDD201 pKa = 3.25 WAEE204 pKa = 3.78 NNLTVFNGGGYY215 pKa = 9.41 VAGIGTDD222 pKa = 3.75 FASGLEE228 pKa = 4.08 GWVYY232 pKa = 10.12 TIVDD236 pKa = 3.75 VNNNIIEE243 pKa = 4.44 PDD245 pKa = 3.64 YY246 pKa = 10.76 PAASYY251 pKa = 9.94 EE252 pKa = 4.08 LQPGDD257 pKa = 3.89 SVVWEE262 pKa = 4.25 YY263 pKa = 11.63 KK264 pKa = 10.4 SLYY267 pKa = 9.82
Molecular weight: 29.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.738
IPC2_protein 3.745
IPC_protein 3.745
Toseland 3.528
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.439
Solomon 3.732
Lehninger 3.681
Nozaki 3.846
DTASelect 4.075
Thurlkill 3.579
EMBOSS 3.668
Sillero 3.872
Patrickios 0.668
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.768
Protein with the highest isoelectric point:
>tr|H5XRQ4|H5XRQ4_9FIRM Sporulation protein YabP OS=Desulfosporosinus youngiae DSM 17734 OX=768710 GN=DesyoDRAFT_0081 PE=4 SV=1
MM1 pKa = 7.36 KK2 pKa = 9.44 RR3 pKa = 11.84 TYY5 pKa = 9.19 QPKK8 pKa = 8.78 NRR10 pKa = 11.84 RR11 pKa = 11.84 HH12 pKa = 5.44 KK13 pKa = 10.09 RR14 pKa = 11.84 VHH16 pKa = 5.93 GFLSRR21 pKa = 11.84 MSTPTGRR28 pKa = 11.84 NVIKK32 pKa = 10.5 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.52 GRR39 pKa = 11.84 KK40 pKa = 8.8 KK41 pKa = 10.81 LSVV44 pKa = 3.15
Molecular weight: 5.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5108
0
5108
1565203
29
4202
306.4
34.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.884 ± 0.041
1.116 ± 0.016
4.98 ± 0.027
6.869 ± 0.047
4.052 ± 0.027
7.46 ± 0.038
1.78 ± 0.017
7.393 ± 0.034
6.131 ± 0.033
10.437 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.658 ± 0.018
4.103 ± 0.027
3.935 ± 0.024
3.749 ± 0.024
4.74 ± 0.038
6.076 ± 0.034
5.283 ± 0.043
7.007 ± 0.028
1.056 ± 0.014
3.29 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here