Roseburia sp. CAG:182

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Roseburia; environmental samples

Average proteome isoelectric point is 5.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2523 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R7QYR2|R7QYR2_9FIRM Transcriptional regulator GntR family OS=Roseburia sp. CAG:182 OX=1262942 GN=BN520_01922 PE=4 SV=1
MM1 pKa = 7.63LANDD5 pKa = 5.25NIPDD9 pKa = 3.88LVSANALSEE18 pKa = 4.38LGGTYY23 pKa = 10.29LDD25 pKa = 3.8QVKK28 pKa = 8.6STNSDD33 pKa = 3.79TIVTSVTYY41 pKa = 10.17KK42 pKa = 10.45DD43 pKa = 3.21SVYY46 pKa = 11.07AFPFTSNTWFMYY58 pKa = 9.22YY59 pKa = 10.33DD60 pKa = 3.42KK61 pKa = 11.38SVFTDD66 pKa = 3.66DD67 pKa = 5.39DD68 pKa = 3.7IKK70 pKa = 11.62SFDD73 pKa = 3.5TMLSKK78 pKa = 11.14GKK80 pKa = 10.7VSFPLSNSWYY90 pKa = 9.02IQAFYY95 pKa = 10.67AGNGCTLFGDD105 pKa = 4.5GTDD108 pKa = 3.45EE109 pKa = 4.56AAGIDD114 pKa = 3.9FGGDD118 pKa = 2.64KK119 pKa = 10.59AAAVTNYY126 pKa = 10.56LVDD129 pKa = 4.63LVANPNFINDD139 pKa = 3.28QDD141 pKa = 4.08GAGIAGLRR149 pKa = 11.84DD150 pKa = 3.41GSVNAIFSGSWDD162 pKa = 3.34AASVKK167 pKa = 10.06EE168 pKa = 4.3ALGDD172 pKa = 3.77NYY174 pKa = 10.59GVAALPTFTVDD185 pKa = 4.64GNEE188 pKa = 4.13CQMKK192 pKa = 10.43SFAGSKK198 pKa = 10.47AIGVNPNCEE207 pKa = 4.06NPQIAMSLAAYY218 pKa = 10.43LSGEE222 pKa = 4.44EE223 pKa = 4.14AQQAHH228 pKa = 5.74YY229 pKa = 10.34DD230 pKa = 3.64EE231 pKa = 6.11RR232 pKa = 11.84NILPSNTNITLADD245 pKa = 4.14DD246 pKa = 5.61PIATATADD254 pKa = 3.87VLDD257 pKa = 4.84HH258 pKa = 6.58CSIMQPIVSAMSNYY272 pKa = 8.63WSPAEE277 pKa = 4.03NMGKK281 pKa = 9.38NLIAGDD287 pKa = 3.9VTHH290 pKa = 7.57DD291 pKa = 3.41NAAEE295 pKa = 4.06KK296 pKa = 10.14TEE298 pKa = 4.06DD299 pKa = 3.71MNTAMNTDD307 pKa = 3.18VAADD311 pKa = 3.56AEE313 pKa = 4.57EE314 pKa = 4.17ATEE317 pKa = 3.94

Molecular weight: 33.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R7QVM5|R7QVM5_9FIRM 6-O-methylguanine DNA methyltransferase DNA binding domain protein OS=Roseburia sp. CAG:182 OX=1262942 GN=BN520_01757 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84RR3 pKa = 11.84TVFMCIEE10 pKa = 4.95LIFFCWFFLPLLLKK24 pKa = 10.98GILNVGNFAGMCLSALALFYY44 pKa = 10.17TGKK47 pKa = 10.2RR48 pKa = 11.84EE49 pKa = 4.11YY50 pKa = 11.14VSGLLSMLRR59 pKa = 11.84EE60 pKa = 3.89NRR62 pKa = 11.84GFCIAEE68 pKa = 3.81WVVRR72 pKa = 11.84IAAVAVCLLLAVSAVRR88 pKa = 11.84IAAAGYY94 pKa = 10.01KK95 pKa = 9.59KK96 pKa = 10.8APDD99 pKa = 3.39TKK101 pKa = 9.56KK102 pKa = 10.34RR103 pKa = 11.84RR104 pKa = 11.84IRR106 pKa = 11.84RR107 pKa = 11.84HH108 pKa = 5.7RR109 pKa = 11.84SSCLAVKK116 pKa = 10.29

Molecular weight: 13.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2523

0

2523

732898

29

2529

290.5

32.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.908 ± 0.051

1.488 ± 0.023

5.911 ± 0.042

7.902 ± 0.06

4.126 ± 0.04

7.023 ± 0.04

1.757 ± 0.022

7.162 ± 0.05

6.972 ± 0.04

8.835 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.177 ± 0.025

4.196 ± 0.036

3.115 ± 0.028

3.174 ± 0.03

4.33 ± 0.034

5.469 ± 0.041

5.42 ± 0.04

7.035 ± 0.04

0.849 ± 0.019

4.143 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski