Amino acid dipepetide frequency for Roseburia sp. CAG:182

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.645AlaAla: 7.645 ± 0.128	1.175AlaCys: 1.175 ± 0.037	5.429AlaAsp: 5.429 ± 0.097	6.069AlaGlu: 6.069 ± 0.106	3.241AlaPhe: 3.241 ± 0.066	6.57AlaGly: 6.57 ± 0.093	1.161AlaHis: 1.161 ± 0.04	4.784AlaIle: 4.784 ± 0.086	5.552AlaLys: 5.552 ± 0.112	7.192AlaLeu: 7.192 ± 0.107	2.408AlaMet: 2.408 ± 0.054	2.738AlaAsn: 2.738 ± 0.075	1.965AlaPro: 1.965 ± 0.056	2.33AlaGln: 2.33 ± 0.065	2.98AlaArg: 2.98 ± 0.074	3.965AlaSer: 3.965 ± 0.087	3.343AlaThr: 3.343 ± 0.082	6.765AlaVal: 6.765 ± 0.104	0.685AlaTrp: 0.685 ± 0.032	3.073AlaTyr: 3.073 ± 0.069	0.01AlaXaa: 0.01 ± 0.003
Cys	1.227CysAla: 1.227 ± 0.042	0.276CysCys: 0.276 ± 0.018	0.819CysAsp: 0.819 ± 0.039	0.966CysGlu: 0.966 ± 0.04	0.686CysPhe: 0.686 ± 0.028	1.584CysGly: 1.584 ± 0.056	0.297CysHis: 0.297 ± 0.018	1.097CysIle: 1.097 ± 0.044	0.763CysLys: 0.763 ± 0.031	1.123CysLeu: 1.123 ± 0.04	0.539CysMet: 0.539 ± 0.029	0.529CysAsn: 0.529 ± 0.026	0.547CysPro: 0.547 ± 0.03	0.326CysGln: 0.326 ± 0.021	0.727CysArg: 0.727 ± 0.031	0.786CysSer: 0.786 ± 0.038	0.66CysThr: 0.66 ± 0.032	1.168CysVal: 1.168 ± 0.037	0.128CysTrp: 0.128 ± 0.014	0.629CysTyr: 0.629 ± 0.03	0.001CysXaa: 0.001 ± 0.001
Asp	5.068AspAla: 5.068 ± 0.092	0.797AspCys: 0.797 ± 0.032	3.119AspAsp: 3.119 ± 0.077	4.908AspGlu: 4.908 ± 0.09	2.722AspPhe: 2.722 ± 0.055	4.811AspGly: 4.811 ± 0.103	1.079AspHis: 1.079 ± 0.04	4.578AspIle: 4.578 ± 0.082	3.303AspLys: 3.303 ± 0.083	4.894AspLeu: 4.894 ± 0.092	1.955AspMet: 1.955 ± 0.057	2.025AspAsn: 2.025 ± 0.053	1.79AspPro: 1.79 ± 0.049	1.515AspGln: 1.515 ± 0.047	2.569AspArg: 2.569 ± 0.056	2.777AspSer: 2.777 ± 0.075	3.508AspThr: 3.508 ± 0.091	4.134AspVal: 4.134 ± 0.075	0.549AspTrp: 0.549 ± 0.034	3.014AspTyr: 3.014 ± 0.076	0.0AspXaa: 0.0 ± 0.0
Glu	5.911GluAla: 5.911 ± 0.107	0.824GluCys: 0.824 ± 0.032	4.634GluAsp: 4.634 ± 0.09	7.805GluGlu: 7.805 ± 0.144	2.558GluPhe: 2.558 ± 0.051	4.074GluGly: 4.074 ± 0.086	1.583GluHis: 1.583 ± 0.052	5.808GluIle: 5.808 ± 0.108	7.764GluLys: 7.764 ± 0.119	6.871GluLeu: 6.871 ± 0.121	2.547GluMet: 2.547 ± 0.052	4.133GluAsn: 4.133 ± 0.077	2.045GluPro: 2.045 ± 0.058	3.286GluGln: 3.286 ± 0.075	3.53GluArg: 3.53 ± 0.094	3.295GluSer: 3.295 ± 0.071	4.137GluThr: 4.137 ± 0.084	4.748GluVal: 4.748 ± 0.075	0.602GluTrp: 0.602 ± 0.029	2.868GluTyr: 2.868 ± 0.056	0.0GluXaa: 0.0 ± 0.0
Phe	3.498PheAla: 3.498 ± 0.07	0.789PheCys: 0.789 ± 0.036	2.571PheAsp: 2.571 ± 0.066	2.629PheGlu: 2.629 ± 0.066	2.053PhePhe: 2.053 ± 0.067	3.085PheGly: 3.085 ± 0.074	0.871PheHis: 0.871 ± 0.034	2.497PheIle: 2.497 ± 0.06	1.902PheLys: 1.902 ± 0.054	4.175PheLeu: 4.175 ± 0.083	1.173PheMet: 1.173 ± 0.038	1.317PheAsn: 1.317 ± 0.041	1.371PhePro: 1.371 ± 0.041	1.202PheGln: 1.202 ± 0.041	1.727PheArg: 1.727 ± 0.045	2.854PheSer: 2.854 ± 0.07	2.317PheThr: 2.317 ± 0.056	3.02PheVal: 3.02 ± 0.073	0.385PheTrp: 0.385 ± 0.024	1.824PheTyr: 1.824 ± 0.058	0.0PheXaa: 0.0 ± 0.0
Gly	5.216GlyAla: 5.216 ± 0.093	1.277GlyCys: 1.277 ± 0.044	3.569GlyAsp: 3.569 ± 0.075	4.848GlyGlu: 4.848 ± 0.094	3.093GlyPhe: 3.093 ± 0.066	4.619GlyGly: 4.619 ± 0.093	1.197GlyHis: 1.197 ± 0.045	6.186GlyIle: 6.186 ± 0.109	5.56GlyLys: 5.56 ± 0.087	5.538GlyLeu: 5.538 ± 0.095	2.637GlyMet: 2.637 ± 0.064	3.142GlyAsn: 3.142 ± 0.073	1.086GlyPro: 1.086 ± 0.046	2.129GlyGln: 2.129 ± 0.062	2.899GlyArg: 2.899 ± 0.075	3.917GlySer: 3.917 ± 0.072	4.242GlyThr: 4.242 ± 0.079	5.154GlyVal: 5.154 ± 0.089	0.666GlyTrp: 0.666 ± 0.032	3.254GlyTyr: 3.254 ± 0.072	0.004GlyXaa: 0.004 ± 0.003
His	1.349HisAla: 1.349 ± 0.046	0.292HisCys: 0.292 ± 0.022	0.886HisAsp: 0.886 ± 0.033	1.115HisGlu: 1.115 ± 0.044	0.864HisPhe: 0.864 ± 0.035	1.276HisGly: 1.276 ± 0.046	0.437HisHis: 0.437 ± 0.03	1.439HisIle: 1.439 ± 0.046	0.916HisLys: 0.916 ± 0.036	1.495HisLeu: 1.495 ± 0.049	0.626HisMet: 0.626 ± 0.029	0.659HisAsn: 0.659 ± 0.024	0.888HisPro: 0.888 ± 0.035	0.487HisGln: 0.487 ± 0.027	0.775HisArg: 0.775 ± 0.032	0.853HisSer: 0.853 ± 0.037	1.004HisThr: 1.004 ± 0.037	1.221HisVal: 1.221 ± 0.043	0.18HisTrp: 0.18 ± 0.017	0.806HisTyr: 0.806 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	5.894IleAla: 5.894 ± 0.1	1.378IleCys: 1.378 ± 0.049	3.953IleAsp: 3.953 ± 0.081	4.92IleGlu: 4.92 ± 0.093	2.818IlePhe: 2.818 ± 0.068	5.203IleGly: 5.203 ± 0.103	1.322IleHis: 1.322 ± 0.045	4.534IleIle: 4.534 ± 0.103	4.003IleLys: 4.003 ± 0.072	7.14IleLeu: 7.14 ± 0.116	1.899IleMet: 1.899 ± 0.055	2.71IleAsn: 2.71 ± 0.057	2.909IlePro: 2.909 ± 0.065	2.141IleGln: 2.141 ± 0.053	3.833IleArg: 3.833 ± 0.085	4.579IleSer: 4.579 ± 0.095	4.136IleThr: 4.136 ± 0.084	4.908IleVal: 4.908 ± 0.091	0.617IleTrp: 0.617 ± 0.028	2.714IleTyr: 2.714 ± 0.062	0.005IleXaa: 0.005 ± 0.003
Lys	4.78LysAla: 4.78 ± 0.104	0.69LysCys: 0.69 ± 0.029	4.145LysAsp: 4.145 ± 0.079	7.519LysGlu: 7.519 ± 0.127	1.976LysPhe: 1.976 ± 0.05	3.782LysGly: 3.782 ± 0.072	1.012LysHis: 1.012 ± 0.035	5.104LysIle: 5.104 ± 0.089	7.152LysLys: 7.152 ± 0.106	5.26LysLeu: 5.26 ± 0.094	2.296LysMet: 2.296 ± 0.057	3.878LysAsn: 3.878 ± 0.077	1.927LysPro: 1.927 ± 0.056	2.556LysGln: 2.556 ± 0.057	3.317LysArg: 3.317 ± 0.074	3.186LysSer: 3.186 ± 0.068	3.804LysThr: 3.804 ± 0.084	4.177LysVal: 4.177 ± 0.075	0.532LysTrp: 0.532 ± 0.03	2.627LysTyr: 2.627 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	7.281LeuAla: 7.281 ± 0.113	1.509LeuCys: 1.509 ± 0.047	5.216LeuAsp: 5.216 ± 0.097	6.102LeuGlu: 6.102 ± 0.1	3.83LeuPhe: 3.83 ± 0.088	5.754LeuGly: 5.754 ± 0.09	1.553LeuHis: 1.553 ± 0.048	5.907LeuIle: 5.907 ± 0.102	6.33LeuLys: 6.33 ± 0.103	8.475LeuLeu: 8.475 ± 0.132	2.52LeuMet: 2.52 ± 0.066	3.861LeuAsn: 3.861 ± 0.075	3.312LeuPro: 3.312 ± 0.068	2.541LeuGln: 2.541 ± 0.055	3.58LeuArg: 3.58 ± 0.078	6.077LeuSer: 6.077 ± 0.105	4.957LeuThr: 4.957 ± 0.091	5.576LeuVal: 5.576 ± 0.079	0.655LeuTrp: 0.655 ± 0.032	3.316LeuTyr: 3.316 ± 0.068	0.004LeuXaa: 0.004 ± 0.003
Met	2.542MetAla: 2.542 ± 0.052	0.329MetCys: 0.329 ± 0.021	2.059MetAsp: 2.059 ± 0.059	2.691MetGlu: 2.691 ± 0.058	1.139MetPhe: 1.139 ± 0.043	1.972MetGly: 1.972 ± 0.05	0.557MetHis: 0.557 ± 0.026	2.223MetIle: 2.223 ± 0.066	2.61MetLys: 2.61 ± 0.065	2.812MetLeu: 2.812 ± 0.07	0.917MetMet: 0.917 ± 0.04	1.502MetAsn: 1.502 ± 0.048	1.143MetPro: 1.143 ± 0.036	1.313MetGln: 1.313 ± 0.044	1.39MetArg: 1.39 ± 0.048	1.721MetSer: 1.721 ± 0.052	1.804MetThr: 1.804 ± 0.047	1.953MetVal: 1.953 ± 0.054	0.21MetTrp: 0.21 ± 0.02	0.888MetTyr: 0.888 ± 0.037	0.0MetXaa: 0.0 ± 0.0
Asn	3.245AsnAla: 3.245 ± 0.066	0.633AsnCys: 0.633 ± 0.034	2.089AsnAsp: 2.089 ± 0.057	3.028AsnGlu: 3.028 ± 0.072	1.562AsnPhe: 1.562 ± 0.046	3.533AsnGly: 3.533 ± 0.082	0.812AsnHis: 0.812 ± 0.033	3.178AsnIle: 3.178 ± 0.072	2.318AsnLys: 2.318 ± 0.057	3.571AsnLeu: 3.571 ± 0.073	1.42AsnMet: 1.42 ± 0.045	1.708AsnAsn: 1.708 ± 0.064	1.778AsnPro: 1.778 ± 0.048	1.34AsnGln: 1.34 ± 0.049	2.049AsnArg: 2.049 ± 0.052	2.133AsnSer: 2.133 ± 0.055	2.363AsnThr: 2.363 ± 0.056	2.85AsnVal: 2.85 ± 0.066	0.363AsnTrp: 0.363 ± 0.024	1.987AsnTyr: 1.987 ± 0.06	0.0AsnXaa: 0.0 ± 0.0
Pro	2.365ProAla: 2.365 ± 0.066	0.442ProCys: 0.442 ± 0.028	2.343ProAsp: 2.343 ± 0.06	3.277ProGlu: 3.277 ± 0.074	1.434ProPhe: 1.434 ± 0.048	2.12ProGly: 2.12 ± 0.052	0.499ProHis: 0.499 ± 0.024	1.872ProIle: 1.872 ± 0.046	1.972ProLys: 1.972 ± 0.054	2.561ProLeu: 2.561 ± 0.069	0.85ProMet: 0.85 ± 0.033	1.137ProAsn: 1.137 ± 0.046	0.67ProPro: 0.67 ± 0.034	1.011ProGln: 1.011 ± 0.038	0.92ProArg: 0.92 ± 0.04	1.566ProSer: 1.566 ± 0.047	1.416ProThr: 1.416 ± 0.046	2.916ProVal: 2.916 ± 0.069	0.296ProTrp: 0.296 ± 0.023	1.479ProTyr: 1.479 ± 0.046	0.003ProXaa: 0.003 ± 0.002
Gln	2.539GlnAla: 2.539 ± 0.057	0.311GlnCys: 0.311 ± 0.02	1.577GlnAsp: 1.577 ± 0.047	2.471GlnGlu: 2.471 ± 0.058	1.243GlnPhe: 1.243 ± 0.043	1.872GlnGly: 1.872 ± 0.053	0.433GlnHis: 0.433 ± 0.027	2.753GlnIle: 2.753 ± 0.066	2.803GlnLys: 2.803 ± 0.067	2.607GlnLeu: 2.607 ± 0.066	1.341GlnMet: 1.341 ± 0.042	1.55GlnAsn: 1.55 ± 0.053	0.86GlnPro: 0.86 ± 0.036	1.063GlnGln: 1.063 ± 0.048	1.161GlnArg: 1.161 ± 0.042	1.555GlnSer: 1.555 ± 0.057	1.838GlnThr: 1.838 ± 0.053	2.152GlnVal: 2.152 ± 0.062	0.255GlnTrp: 0.255 ± 0.023	1.356GlnTyr: 1.356 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.924ArgAla: 2.924 ± 0.067	0.595ArgCys: 0.595 ± 0.03	2.325ArgAsp: 2.325 ± 0.057	4.001ArgGlu: 4.001 ± 0.099	1.741ArgPhe: 1.741 ± 0.049	2.493ArgGly: 2.493 ± 0.06	0.776ArgHis: 0.776 ± 0.036	3.423ArgIle: 3.423 ± 0.078	3.452ArgLys: 3.452 ± 0.076	3.702ArgLeu: 3.702 ± 0.082	1.714ArgMet: 1.714 ± 0.05	1.841ArgAsn: 1.841 ± 0.051	1.277ArgPro: 1.277 ± 0.043	1.646ArgGln: 1.646 ± 0.047	2.157ArgArg: 2.157 ± 0.065	2.184ArgSer: 2.184 ± 0.053	2.18ArgThr: 2.18 ± 0.046	2.71ArgVal: 2.71 ± 0.06	0.33ArgTrp: 0.33 ± 0.023	1.826ArgTyr: 1.826 ± 0.049	0.001ArgXaa: 0.001 ± 0.001
Ser	4.318SerAla: 4.318 ± 0.09	0.824SerCys: 0.824 ± 0.033	3.488SerAsp: 3.488 ± 0.08	3.774SerGlu: 3.774 ± 0.074	2.571SerPhe: 2.571 ± 0.067	4.795SerGly: 4.795 ± 0.089	0.947SerHis: 0.947 ± 0.038	3.815SerIle: 3.815 ± 0.076	2.854SerLys: 2.854 ± 0.066	4.675SerLeu: 4.675 ± 0.087	1.877SerMet: 1.877 ± 0.052	2.075SerAsn: 2.075 ± 0.057	1.512SerPro: 1.512 ± 0.048	1.562SerGln: 1.562 ± 0.045	2.377SerArg: 2.377 ± 0.055	3.212SerSer: 3.212 ± 0.096	2.661SerThr: 2.661 ± 0.071	4.257SerVal: 4.257 ± 0.088	0.505SerTrp: 0.505 ± 0.026	2.587SerTyr: 2.587 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	4.459ThrAla: 4.459 ± 0.089	0.716ThrCys: 0.716 ± 0.031	3.629ThrAsp: 3.629 ± 0.072	4.409ThrGlu: 4.409 ± 0.093	2.154ThrPhe: 2.154 ± 0.057	4.751ThrGly: 4.751 ± 0.092	0.868ThrHis: 0.868 ± 0.031	3.687ThrIle: 3.687 ± 0.069	3.153ThrLys: 3.153 ± 0.073	4.725ThrLeu: 4.725 ± 0.085	1.45ThrMet: 1.45 ± 0.047	2.033ThrAsn: 2.033 ± 0.059	1.993ThrPro: 1.993 ± 0.056	1.525ThrGln: 1.525 ± 0.051	1.961ThrArg: 1.961 ± 0.047	2.797ThrSer: 2.797 ± 0.072	2.801ThrThr: 2.801 ± 0.086	4.462ThrVal: 4.462 ± 0.091	0.469ThrTrp: 0.469 ± 0.029	2.152ThrTyr: 2.152 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	5.312ValAla: 5.312 ± 0.086	1.285ValCys: 1.285 ± 0.049	4.025ValAsp: 4.025 ± 0.077	4.578ValGlu: 4.578 ± 0.091	3.232ValPhe: 3.232 ± 0.072	4.381ValGly: 4.381 ± 0.076	1.1ValHis: 1.1 ± 0.037	5.325ValIle: 5.325 ± 0.093	4.478ValLys: 4.478 ± 0.093	6.731ValLeu: 6.731 ± 0.103	2.145ValMet: 2.145 ± 0.053	2.863ValAsn: 2.863 ± 0.067	2.497ValPro: 2.497 ± 0.063	1.841ValGln: 1.841 ± 0.053	2.894ValArg: 2.894 ± 0.064	4.732ValSer: 4.732 ± 0.085	4.488ValThr: 4.488 ± 0.082	5.017ValVal: 5.017 ± 0.089	0.626ValTrp: 0.626 ± 0.031	2.796ValTyr: 2.796 ± 0.065	0.001ValXaa: 0.001 ± 0.001
Trp	0.499TrpAla: 0.499 ± 0.026	0.145TrpCys: 0.145 ± 0.016	0.516TrpAsp: 0.516 ± 0.03	0.701TrpGlu: 0.701 ± 0.032	0.377TrpPhe: 0.377 ± 0.024	0.574TrpGly: 0.574 ± 0.027	0.165TrpHis: 0.165 ± 0.015	0.674TrpIle: 0.674 ± 0.029	0.634TrpLys: 0.634 ± 0.027	0.827TrpLeu: 0.827 ± 0.041	0.315TrpMet: 0.315 ± 0.021	0.524TrpAsn: 0.524 ± 0.028	0.18TrpPro: 0.18 ± 0.016	0.345TrpGln: 0.345 ± 0.024	0.322TrpArg: 0.322 ± 0.022	0.448TrpSer: 0.448 ± 0.027	0.356TrpThr: 0.356 ± 0.025	0.457TrpVal: 0.457 ± 0.025	0.087TrpTrp: 0.087 ± 0.011	0.341TrpTyr: 0.341 ± 0.023	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.005TyrAla: 3.005 ± 0.064	0.592TyrCys: 0.592 ± 0.027	2.722TyrAsp: 2.722 ± 0.069	3.212TyrGlu: 3.212 ± 0.081	1.966TyrPhe: 1.966 ± 0.053	2.985TyrGly: 2.985 ± 0.073	0.901TyrHis: 0.901 ± 0.046	2.73TyrIle: 2.73 ± 0.07	2.198TyrLys: 2.198 ± 0.054	3.979TyrLeu: 3.979 ± 0.083	1.137TyrMet: 1.137 ± 0.041	1.734TyrAsn: 1.734 ± 0.052	1.394TyrPro: 1.394 ± 0.046	1.584TyrGln: 1.584 ± 0.047	2.123TyrArg: 2.123 ± 0.051	2.047TyrSer: 2.047 ± 0.049	2.178TyrThr: 2.178 ± 0.057	2.7TyrVal: 2.7 ± 0.063	0.348TyrTrp: 0.348 ± 0.027	1.895TyrTyr: 1.895 ± 0.064	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.004XaaAla: 0.004 ± 0.003	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.003XaaLys: 0.003 ± 0.002	0.001XaaLeu: 0.001 ± 0.001	0.003XaaMet: 0.003 ± 0.002	0.0XaaAsn: 0.0 ± 0.0	0.004XaaPro: 0.004 ± 0.002	0.0XaaGln: 0.0 ± 0.0	0.005XaaArg: 0.005 ± 0.003	0.001XaaSer: 0.001 ± 0.001	0.003XaaThr: 0.003 ± 0.002	0.003XaaVal: 0.003 ± 0.002	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.046XaaXaa: 0.046 ± 0.011

Statistics based on 2523 proteins (732898 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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