Teviot virus
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5I663|A0A0C5I663_9MONO Fusion glycoprotein F0 OS=Teviot virus OX=1554501 GN=F PE=2 SV=1
MM1 pKa = 7.4 SSVFRR6 pKa = 11.84 AFEE9 pKa = 4.3 LFTLEE14 pKa = 4.45 QEE16 pKa = 4.23 QHH18 pKa = 5.83 EE19 pKa = 4.66 LGNDD23 pKa = 2.97 IEE25 pKa = 5.09 LPPEE29 pKa = 3.83 TLRR32 pKa = 11.84 ANIKK36 pKa = 9.89 VCILNSQDD44 pKa = 3.01 PQTRR48 pKa = 11.84 HH49 pKa = 7.0 DD50 pKa = 4.01 MMCFCLRR57 pKa = 11.84 LIASNSARR65 pKa = 11.84 AAHH68 pKa = 6.75 KK69 pKa = 10.53 AGAILTLLSLPTAMMQNHH87 pKa = 5.88 IRR89 pKa = 11.84 IADD92 pKa = 4.24 RR93 pKa = 11.84 SPDD96 pKa = 3.38 ADD98 pKa = 3.22 IEE100 pKa = 4.36 RR101 pKa = 11.84 IEE103 pKa = 4.01 VDD105 pKa = 3.08 GFEE108 pKa = 4.55 PGTYY112 pKa = 9.65 RR113 pKa = 11.84 LRR115 pKa = 11.84 PNARR119 pKa = 11.84 TPLTNGEE126 pKa = 3.91 ITALDD131 pKa = 4.15 LMANDD136 pKa = 5.72 LPDD139 pKa = 3.74 TYY141 pKa = 11.39 TNDD144 pKa = 3.5 TPFVNHH150 pKa = 5.55 RR151 pKa = 11.84 TEE153 pKa = 5.23 GEE155 pKa = 4.07 NCDD158 pKa = 3.55 EE159 pKa = 4.39 TEE161 pKa = 3.87 QFLNAIYY168 pKa = 10.52 SVLVQLWVMVCKK180 pKa = 10.65 CMTAHH185 pKa = 6.77 DD186 pKa = 4.33 QPTGSDD192 pKa = 3.06 EE193 pKa = 4.13 RR194 pKa = 11.84 RR195 pKa = 11.84 LAKK198 pKa = 10.15 YY199 pKa = 7.98 QQQGRR204 pKa = 11.84 MDD206 pKa = 3.43 QKK208 pKa = 11.1 YY209 pKa = 10.31 ALQPEE214 pKa = 5.03 LRR216 pKa = 11.84 RR217 pKa = 11.84 QIQTCIRR224 pKa = 11.84 RR225 pKa = 11.84 SLTIRR230 pKa = 11.84 QFLTYY235 pKa = 10.1 EE236 pKa = 3.87 LQTARR241 pKa = 11.84 KK242 pKa = 8.48 QGAITGRR249 pKa = 11.84 YY250 pKa = 7.34 YY251 pKa = 11.77 AMVGDD256 pKa = 3.52 IGKK259 pKa = 10.43 YY260 pKa = 9.05 IDD262 pKa = 3.75 NAGMSAFFMTMRR274 pKa = 11.84 FALGTRR280 pKa = 11.84 WPPLALSAFSGEE292 pKa = 4.03 LLKK295 pKa = 11.09 LKK297 pKa = 10.68 SLMQLYY303 pKa = 10.58 RR304 pKa = 11.84 NLGEE308 pKa = 4.16 KK309 pKa = 9.58 ARR311 pKa = 11.84 YY312 pKa = 7.03 MALLEE317 pKa = 4.25 MPEE320 pKa = 4.02 MMEE323 pKa = 4.34 FAPANYY329 pKa = 8.35 PLCYY333 pKa = 9.75 SYY335 pKa = 11.89 AMGIGSVQDD344 pKa = 3.3 PMMRR348 pKa = 11.84 NYY350 pKa = 9.81 TFARR354 pKa = 11.84 PFLNPAYY361 pKa = 9.55 FQLGVEE367 pKa = 4.29 TANRR371 pKa = 11.84 QQGSVDD377 pKa = 3.14 KK378 pKa = 11.57 NMAEE382 pKa = 3.98 EE383 pKa = 4.49 LGLTEE388 pKa = 4.13 EE389 pKa = 4.56 EE390 pKa = 4.2 RR391 pKa = 11.84 RR392 pKa = 11.84 DD393 pKa = 3.35 MSATVTRR400 pKa = 11.84 LTTGRR405 pKa = 11.84 GAGQAQDD412 pKa = 4.28 MINIMGARR420 pKa = 11.84 QAAGGRR426 pKa = 11.84 AAQGRR431 pKa = 11.84 ALRR434 pKa = 11.84 VIEE437 pKa = 4.14 EE438 pKa = 4.45 DD439 pKa = 3.18 EE440 pKa = 4.4 TTEE443 pKa = 4.36 EE444 pKa = 4.25 EE445 pKa = 4.6 SDD447 pKa = 5.6 DD448 pKa = 4.41 EE449 pKa = 4.7 IQDD452 pKa = 3.81 EE453 pKa = 4.78 VQGRR457 pKa = 11.84 PLPPLPAQVRR467 pKa = 11.84 EE468 pKa = 4.23 VDD470 pKa = 3.07 WEE472 pKa = 3.94 ARR474 pKa = 11.84 LAEE477 pKa = 4.35 IEE479 pKa = 4.14 EE480 pKa = 4.33 QEE482 pKa = 3.8 QRR484 pKa = 11.84 MRR486 pKa = 11.84 DD487 pKa = 3.2 RR488 pKa = 11.84 GQGMGGGAPATGPAQPRR505 pKa = 11.84 QALHH509 pKa = 6.4 EE510 pKa = 4.24 EE511 pKa = 4.26 QVLLDD516 pKa = 3.97 LDD518 pKa = 3.82 MM519 pKa = 6.33
Molecular weight: 58.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.975
IPC2_protein 5.003
IPC_protein 4.952
Toseland 4.838
ProMoST 5.054
Dawson 4.902
Bjellqvist 5.041
Wikipedia 4.749
Rodwell 4.813
Grimsley 4.749
Solomon 4.902
Lehninger 4.851
Nozaki 5.003
DTASelect 5.143
Thurlkill 4.825
EMBOSS 4.774
Sillero 5.08
Patrickios 4.088
IPC_peptide 4.914
IPC2_peptide 5.08
IPC2.peptide.svr19 5.063
Protein with the highest isoelectric point:
>tr|A0A0C5HWD1|A0A0C5HWD1_9MONO Isoform of A0A0C5I317 Non-structural protein V OS=Teviot virus OX=1554501 GN=P PE=2 SV=1
MM1 pKa = 8.01 ALRR4 pKa = 11.84 QATIPIAVDD13 pKa = 3.26 NEE15 pKa = 4.47 SEE17 pKa = 4.34 KK18 pKa = 11.46 NNLNPFPIVPITRR31 pKa = 11.84 DD32 pKa = 3.46 DD33 pKa = 3.99 GSPTGRR39 pKa = 11.84 LVRR42 pKa = 11.84 QLRR45 pKa = 11.84 IKK47 pKa = 10.72 NLTPRR52 pKa = 11.84 GSTEE56 pKa = 4.25 LPLTFINTYY65 pKa = 10.73 GFIKK69 pKa = 10.48 PLMTYY74 pKa = 8.78 TEE76 pKa = 5.34 FYY78 pKa = 10.97 SEE80 pKa = 4.49 LHH82 pKa = 6.39 HH83 pKa = 7.12 QSSTPCLTACMIPFGAGPYY102 pKa = 9.53 IEE104 pKa = 4.61 NPHH107 pKa = 6.93 RR108 pKa = 11.84 ILDD111 pKa = 3.79 EE112 pKa = 4.25 CDD114 pKa = 2.94 KK115 pKa = 11.45 VNIVVRR121 pKa = 11.84 KK122 pKa = 7.75 SASVKK127 pKa = 10.25 EE128 pKa = 4.11 EE129 pKa = 3.75 IIFDD133 pKa = 3.88 VRR135 pKa = 11.84 RR136 pKa = 11.84 LPPLFNRR143 pKa = 11.84 HH144 pKa = 5.38 QISGNRR150 pKa = 11.84 LICVPSEE157 pKa = 4.41 KK158 pKa = 10.2 YY159 pKa = 10.28 VKK161 pKa = 10.7 SPGKK165 pKa = 8.02 MTAGTDD171 pKa = 3.42 YY172 pKa = 10.48 SYY174 pKa = 11.41 QIAFVSLTFCPEE186 pKa = 3.79 SQKK189 pKa = 10.37 FRR191 pKa = 11.84 VARR194 pKa = 11.84 PLQTIRR200 pKa = 11.84 SPIMRR205 pKa = 11.84 SVQLEE210 pKa = 4.33 VILKK214 pKa = 9.41 VDD216 pKa = 4.23 CAANSPLKK224 pKa = 10.5 RR225 pKa = 11.84 FLIVSPDD232 pKa = 3.25 SKK234 pKa = 11.26 EE235 pKa = 3.86 YY236 pKa = 9.75 FASVWFHH243 pKa = 5.17 VCNLYY248 pKa = 10.58 RR249 pKa = 11.84 GNKK252 pKa = 8.18 PFKK255 pKa = 10.75 SYY257 pKa = 11.46 DD258 pKa = 3.2 DD259 pKa = 3.97 TYY261 pKa = 11.31 FSEE264 pKa = 4.69 KK265 pKa = 9.41 CRR267 pKa = 11.84 AMQLEE272 pKa = 4.42 CGIVDD277 pKa = 3.09 MWGPTLVVKK286 pKa = 10.71 AHH288 pKa = 5.64 GKK290 pKa = 7.5 IPKK293 pKa = 7.84 MAKK296 pKa = 9.8 PFFSSKK302 pKa = 9.4 GWSCHH307 pKa = 5.46 AFADD311 pKa = 4.71 SAPTLAKK318 pKa = 10.59 ALWSVGAQITQVNAILQPSDD338 pKa = 3.11 LHH340 pKa = 7.72 QLVQVSDD347 pKa = 4.26 VIWPKK352 pKa = 10.83 VKK354 pKa = 10.31 LDD356 pKa = 3.93 EE357 pKa = 5.7 KK358 pKa = 9.84 IQLYY362 pKa = 9.98 AAAKK366 pKa = 7.72 WNPFKK371 pKa = 10.99 KK372 pKa = 10.3 SANN375 pKa = 3.57
Molecular weight: 42.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.76
IPC2_protein 8.609
IPC_protein 8.478
Toseland 9.136
ProMoST 9.019
Dawson 9.472
Bjellqvist 9.341
Wikipedia 9.604
Rodwell 9.706
Grimsley 9.472
Solomon 9.516
Lehninger 9.487
Nozaki 9.531
DTASelect 9.224
Thurlkill 9.355
EMBOSS 9.619
Sillero 9.531
Patrickios 4.774
IPC_peptide 9.516
IPC2_peptide 8.112
IPC2.peptide.svr19 7.799
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
1
7
4909
226
2271
701.3
78.47
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.233 ± 0.93
2.282 ± 0.287
5.154 ± 0.46
5.195 ± 0.664
3.504 ± 0.446
4.909 ± 0.543
1.833 ± 0.176
7.15 ± 0.477
4.807 ± 0.474
10.959 ± 1.401
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.546 ± 0.417
5.174 ± 0.476
5.378 ± 0.627
4.665 ± 0.502
5.072 ± 0.548
9.146 ± 0.99
6.03 ± 0.24
5.643 ± 0.565
1.08 ± 0.167
3.239 ± 0.309
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here