Thiohalorhabdus denitrificans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Thiohalorhabdus

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2692 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G5GVC9|A0A1G5GVC9_9GAMM Competence protein ComEA OS=Thiohalorhabdus denitrificans OX=381306 GN=SAMN05661077_2470 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84RR3 pKa = 11.84IQNGPAAEE11 pKa = 4.19TGHH14 pKa = 7.22ADD16 pKa = 3.18GTAAPGRR23 pKa = 11.84LRR25 pKa = 11.84HH26 pKa = 5.89HH27 pKa = 6.52VLPGLALGLLLLPAPAGAADD47 pKa = 4.85DD48 pKa = 4.65CPSDD52 pKa = 4.08ASTTCSVDD60 pKa = 3.06VGSSSEE66 pKa = 4.2GEE68 pKa = 3.83MGGNSDD74 pKa = 3.74TDD76 pKa = 3.37WFKK79 pKa = 10.97VTGLEE84 pKa = 3.82AGKK87 pKa = 8.57TYY89 pKa = 10.7RR90 pKa = 11.84FHH92 pKa = 7.33QKK94 pKa = 10.96GEE96 pKa = 4.23TSDD99 pKa = 3.58EE100 pKa = 4.28TLTLLDD106 pKa = 4.16PYY108 pKa = 11.4ARR110 pKa = 11.84LVDD113 pKa = 3.92ANGTVVAEE121 pKa = 4.93DD122 pKa = 4.98DD123 pKa = 4.11DD124 pKa = 4.36TGPMNNAQITYY135 pKa = 9.9SPVEE139 pKa = 4.15AEE141 pKa = 3.98LPLYY145 pKa = 10.8LEE147 pKa = 5.63ADD149 pKa = 3.97NQPDD153 pKa = 4.3DD154 pKa = 3.48PSGTYY159 pKa = 9.22RR160 pKa = 11.84VSVEE164 pKa = 3.95EE165 pKa = 5.45FVDD168 pKa = 4.03DD169 pKa = 4.16DD170 pKa = 4.42CVDD173 pKa = 3.88RR174 pKa = 11.84PHH176 pKa = 7.04TDD178 pKa = 2.84SDD180 pKa = 4.2CALEE184 pKa = 4.75LDD186 pKa = 4.98TIITWGIDD194 pKa = 3.38FAGDD198 pKa = 3.75EE199 pKa = 4.46DD200 pKa = 4.11WLKK203 pKa = 11.38ASVDD207 pKa = 3.82TSSGPHH213 pKa = 4.93YY214 pKa = 10.28QFSVANSDD222 pKa = 3.72SQDD225 pKa = 2.99VSLEE229 pKa = 3.74LRR231 pKa = 11.84AQDD234 pKa = 3.97GNLHH238 pKa = 6.22IADD241 pKa = 4.41EE242 pKa = 4.78GADD245 pKa = 3.58EE246 pKa = 4.36ATLTFTTTDD255 pKa = 3.32DD256 pKa = 3.7SNYY259 pKa = 9.01FAAVSFLSSEE269 pKa = 4.21TGDD272 pKa = 4.15FSATYY277 pKa = 8.76TNYY280 pKa = 10.79SGGCPSNPLYY290 pKa = 10.3PSALDD295 pKa = 3.47YY296 pKa = 11.65SNYY299 pKa = 8.64STDD302 pKa = 3.62YY303 pKa = 9.79QKK305 pKa = 11.17CGAEE309 pKa = 4.15APGPDD314 pKa = 3.97AGSATLSGSIEE325 pKa = 4.2PVGDD329 pKa = 3.59TDD331 pKa = 3.33WFKK334 pKa = 10.49ITGMQADD341 pKa = 3.41QSYY344 pKa = 10.42QLDD347 pKa = 4.18LTSGTDD353 pKa = 3.67GLPDD357 pKa = 4.32PLLRR361 pKa = 11.84IYY363 pKa = 10.56DD364 pKa = 3.7AEE366 pKa = 4.32GNEE369 pKa = 4.78LAVDD373 pKa = 3.83DD374 pKa = 5.39DD375 pKa = 4.67GGSKK379 pKa = 10.41EE380 pKa = 4.06DD381 pKa = 3.57AQLGFSPPADD391 pKa = 3.2GDD393 pKa = 3.99YY394 pKa = 10.49FASAEE399 pKa = 4.26SSPKK403 pKa = 9.69DD404 pKa = 3.67DD405 pKa = 3.54QRR407 pKa = 11.84GGYY410 pKa = 8.31SLEE413 pKa = 3.87VANYY417 pKa = 10.1SDD419 pKa = 4.38RR420 pKa = 11.84DD421 pKa = 3.97CAGSNATDD429 pKa = 3.65CGIKK433 pKa = 10.07IGDD436 pKa = 3.85TLVADD441 pKa = 5.75GSTSWDD447 pKa = 3.7YY448 pKa = 10.92EE449 pKa = 4.25VHH451 pKa = 6.65VSGDD455 pKa = 3.72SDD457 pKa = 3.42WFAVSGMTAGEE468 pKa = 4.27SYY470 pKa = 10.79KK471 pKa = 10.7FEE473 pKa = 4.46LRR475 pKa = 11.84NTDD478 pKa = 5.02GILQDD483 pKa = 4.61PYY485 pKa = 11.56LSVYY489 pKa = 10.43DD490 pKa = 3.99SNGEE494 pKa = 3.94EE495 pKa = 4.24LRR497 pKa = 11.84SSGLSKK503 pKa = 11.07DD504 pKa = 3.44PAQLGFTPPDD514 pKa = 3.49NGDD517 pKa = 2.94YY518 pKa = 10.37WIAAEE523 pKa = 4.9SGTEE527 pKa = 3.92GDD529 pKa = 3.13TGAYY533 pKa = 8.19EE534 pKa = 4.2LSAGKK539 pKa = 10.48FNDD542 pKa = 3.65DD543 pKa = 4.17HH544 pKa = 7.3ADD546 pKa = 3.8CATSACSPGTLPVDD560 pKa = 4.13DD561 pKa = 4.62SANGEE566 pKa = 4.27LEE568 pKa = 4.33VPGDD572 pKa = 3.84RR573 pKa = 11.84DD574 pKa = 3.18WFKK577 pKa = 11.8VDD579 pKa = 4.48LEE581 pKa = 4.36SGNYY585 pKa = 7.15YY586 pKa = 9.84TFTLSGDD593 pKa = 3.81TLGAPHH599 pKa = 6.21VRR601 pKa = 11.84LRR603 pKa = 11.84TDD605 pKa = 3.63DD606 pKa = 3.95GSILASQAASPDD618 pKa = 3.31SSEE621 pKa = 4.67AKK623 pKa = 8.86VTHH626 pKa = 6.35EE627 pKa = 4.24PGANQTVYY635 pKa = 11.11LEE637 pKa = 4.33AASVEE642 pKa = 4.25DD643 pKa = 4.64EE644 pKa = 4.34SAGTYY649 pKa = 7.63TVSATAQDD657 pKa = 3.98LSGGTGGGSSDD668 pKa = 3.5GGGSGGGGGCALAAEE683 pKa = 4.73PQPLDD688 pKa = 3.25PTLPLLAGLGLAYY701 pKa = 10.61LLRR704 pKa = 11.84RR705 pKa = 11.84GRR707 pKa = 11.84PRR709 pKa = 11.84AA710 pKa = 3.4

Molecular weight: 74.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G5ABI4|A0A1G5ABI4_9GAMM Uncharacterized protein OS=Thiohalorhabdus denitrificans OX=381306 GN=SAMN05661077_0219 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.42HH3 pKa = 5.45TFQPSNIRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 9.21RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.74GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 9.98GGKK28 pKa = 9.08RR29 pKa = 11.84VLKK32 pKa = 10.22RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 9.02RR41 pKa = 11.84LTPP44 pKa = 3.95

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2692

0

2692

850307

33

2529

315.9

34.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.507 ± 0.065

0.754 ± 0.014

5.612 ± 0.039

7.946 ± 0.056

3.369 ± 0.028

9.454 ± 0.046

2.264 ± 0.021

3.646 ± 0.034

2.393 ± 0.031

10.902 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.118 ± 0.021

2.216 ± 0.027

5.643 ± 0.034

3.24 ± 0.034

8.173 ± 0.056

4.418 ± 0.032

4.765 ± 0.028

7.692 ± 0.043

1.467 ± 0.021

2.421 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski