Thermus phage G20c (Thermus thermophilus phage G20c)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Oshimavirus; unclassified Oshimavirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L4BKR2|A0A1L4BKR2_BPG20 Uncharacterized protein OS=Thermus phage G20c OX=1406341 GN=G20c_89 PE=4 SV=1
MM1 pKa = 7.59SEE3 pKa = 4.67LDD5 pKa = 3.49RR6 pKa = 11.84LPYY9 pKa = 10.33LDD11 pKa = 5.15LWPFDD16 pKa = 4.12HH17 pKa = 7.39EE18 pKa = 4.35EE19 pKa = 4.08EE20 pKa = 5.38EE21 pKa = 4.15YY22 pKa = 11.16DD23 pKa = 3.34PWFDD27 pKa = 3.54YY28 pKa = 11.25LLRR31 pKa = 11.84TPPWEE36 pKa = 3.99LPIPEE41 pKa = 4.35EE42 pKa = 5.04DD43 pKa = 3.51EE44 pKa = 4.14

Molecular weight: 5.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L4BKL9|A0A1L4BKL9_BPG20 Uncharacterized protein OS=Thermus phage G20c OX=1406341 GN=G20c_52 PE=4 SV=1
MM1 pKa = 7.55PHH3 pKa = 6.41LTDD6 pKa = 3.38STPWLTASLTRR17 pKa = 11.84TRR19 pKa = 11.84IEE21 pKa = 3.53RR22 pKa = 11.84RR23 pKa = 11.84YY24 pKa = 10.58GKK26 pKa = 8.84IHH28 pKa = 6.0HH29 pKa = 6.92VIAISHH35 pKa = 6.94DD36 pKa = 3.5LAKK39 pKa = 10.41RR40 pKa = 11.84IPFGTEE46 pKa = 3.03VRR48 pKa = 11.84LVCGDD53 pKa = 3.35QVIYY57 pKa = 10.97GLVEE61 pKa = 4.26DD62 pKa = 4.53LMHH65 pKa = 6.47RR66 pKa = 11.84RR67 pKa = 11.84WRR69 pKa = 11.84LRR71 pKa = 11.84VDD73 pKa = 2.59IWFPSLHH80 pKa = 5.0QAKK83 pKa = 10.5AFGVCRR89 pKa = 11.84GYY91 pKa = 11.39LEE93 pKa = 3.89VLKK96 pKa = 9.84PVRR99 pKa = 11.84VIKK102 pKa = 10.3FKK104 pKa = 10.6RR105 pKa = 11.84EE106 pKa = 3.72KK107 pKa = 10.21YY108 pKa = 10.06GPEE111 pKa = 3.53ARR113 pKa = 11.84LQRR116 pKa = 3.97

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

111

0

111

25919

30

5007

233.5

26.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.599 ± 0.59

0.421 ± 0.102

5.243 ± 0.208

7.616 ± 0.449

4.028 ± 0.188

6.79 ± 0.219

1.663 ± 0.212

3.665 ± 0.272

5.093 ± 0.317

11.713 ± 0.38

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.852 ± 0.12

3.156 ± 0.152

5.201 ± 0.36

3.457 ± 0.274

6.632 ± 0.208

5.436 ± 0.268

4.846 ± 0.237

8.06 ± 0.231

1.428 ± 0.224

4.101 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski