Mycobacterium phage Avocado

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 68 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A222YYA0|A0A222YYA0_9CAUD DUF3850 domain-containing protein OS=Mycobacterium phage Avocado OX=2024302 GN=SEA_AVOCADO_37 PE=4 SV=1
MM1 pKa = 7.23AHH3 pKa = 6.13SEE5 pKa = 4.1AMRR8 pKa = 11.84AALDD12 pKa = 3.91ALDD15 pKa = 3.78VAIVDD20 pKa = 3.83LFEE23 pKa = 6.3AIRR26 pKa = 11.84SDD28 pKa = 5.32DD29 pKa = 4.31PDD31 pKa = 4.17DD32 pKa = 4.35TPPDD36 pKa = 5.2AIPVDD41 pKa = 3.97AVLLIGVQHH50 pKa = 7.04IDD52 pKa = 4.56DD53 pKa = 4.82DD54 pKa = 4.36DD55 pKa = 4.95ARR57 pKa = 11.84IGYY60 pKa = 7.39TEE62 pKa = 3.84VVPRR66 pKa = 11.84YY67 pKa = 8.29GAQPAYY73 pKa = 8.62VTRR76 pKa = 11.84GLLDD80 pKa = 3.7DD81 pKa = 4.57ARR83 pKa = 11.84RR84 pKa = 11.84LMDD87 pKa = 3.43QVSDD91 pKa = 5.38RR92 pKa = 11.84GDD94 pKa = 3.22QDD96 pKa = 3.08QGGG99 pKa = 3.48

Molecular weight: 10.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A222YZX3|A0A222YZX3_9CAUD Uncharacterized protein OS=Mycobacterium phage Avocado OX=2024302 GN=SEA_AVOCADO_46 PE=4 SV=1
MM1 pKa = 6.99TTQQGIIVGTIIGALSLVAIVAEE24 pKa = 4.34PPAWAIATFGWIAGNFRR41 pKa = 11.84RR42 pKa = 11.84GRR44 pKa = 11.84HH45 pKa = 5.69HH46 pKa = 7.15LHH48 pKa = 6.64APLPARR54 pKa = 11.84PFEE57 pKa = 4.09RR58 pKa = 11.84EE59 pKa = 3.84RR60 pKa = 11.84LTVAALAGRR69 pKa = 11.84LEE71 pKa = 4.36AEE73 pKa = 3.93ARR75 pKa = 11.84RR76 pKa = 11.84PRR78 pKa = 11.84LVPRR82 pKa = 11.84KK83 pKa = 9.62YY84 pKa = 9.86GRR86 pKa = 11.84GYY88 pKa = 10.18IAIEE92 pKa = 3.85PDD94 pKa = 3.0ASGFSATLARR104 pKa = 11.84VLDD107 pKa = 4.03EE108 pKa = 4.86LDD110 pKa = 3.4AGRR113 pKa = 11.84GIADD117 pKa = 3.77AHH119 pKa = 6.63AGIAGG124 pKa = 3.47

Molecular weight: 13.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

68

0

68

14851

42

1346

218.4

23.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.238 ± 0.589

0.963 ± 0.209

7.461 ± 0.373

5.4 ± 0.36

3.151 ± 0.175

9.447 ± 0.738

1.811 ± 0.173

4.107 ± 0.198

3.427 ± 0.251

7.528 ± 0.251

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.074 ± 0.13

3.252 ± 0.216

6.356 ± 0.37

3.717 ± 0.133

6.888 ± 0.529

4.283 ± 0.295

6.282 ± 0.229

6.68 ± 0.198

1.946 ± 0.174

1.986 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski