Amino acid dipepetide frequency for Eggerthella phage PMBT5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.557AlaAla: 12.557 ± 1.699
0.528AlaCys: 0.528 ± 0.294
7.386AlaAsp: 7.386 ± 1.507
7.175AlaGlu: 7.175 ± 0.938
2.743AlaPhe: 2.743 ± 0.583
6.542AlaGly: 6.542 ± 0.854
1.583AlaHis: 1.583 ± 0.49
8.125AlaIle: 8.125 ± 1.18
6.648AlaLys: 6.648 ± 1.0
8.23AlaLeu: 8.23 ± 1.715
3.166AlaMet: 3.166 ± 0.757
5.487AlaAsn: 5.487 ± 0.886
2.743AlaPro: 2.743 ± 0.459
4.537AlaGln: 4.537 ± 0.993
3.06AlaArg: 3.06 ± 0.657
7.597AlaSer: 7.597 ± 0.902
6.964AlaThr: 6.964 ± 1.343
5.804AlaVal: 5.804 ± 0.746
1.688AlaTrp: 1.688 ± 0.365
4.221AlaTyr: 4.221 ± 0.507
0.0AlaXaa: 0.0 ± 0.0
Cys
1.161CysAla: 1.161 ± 0.368
0.211CysCys: 0.211 ± 0.119
1.055CysAsp: 1.055 ± 0.324
1.583CysGlu: 1.583 ± 0.436
0.844CysPhe: 0.844 ± 0.241
2.321CysGly: 2.321 ± 0.886
0.211CysHis: 0.211 ± 0.137
0.739CysIle: 0.739 ± 0.332
0.739CysLys: 0.739 ± 0.283
1.161CysLeu: 1.161 ± 0.35
0.211CysMet: 0.211 ± 0.144
0.422CysAsn: 0.422 ± 0.311
0.739CysPro: 0.739 ± 0.293
0.317CysGln: 0.317 ± 0.147
0.739CysArg: 0.739 ± 0.391
0.95CysSer: 0.95 ± 0.332
1.161CysThr: 1.161 ± 0.375
1.055CysVal: 1.055 ± 0.316
0.211CysTrp: 0.211 ± 0.229
0.422CysTyr: 0.422 ± 0.208
0.0CysXaa: 0.0 ± 0.0
Asp
9.391AspAla: 9.391 ± 1.073
1.055AspCys: 1.055 ± 0.348
4.748AspAsp: 4.748 ± 0.702
4.115AspGlu: 4.115 ± 0.708
1.899AspPhe: 1.899 ± 0.582
8.864AspGly: 8.864 ± 1.438
0.95AspHis: 0.95 ± 0.369
3.377AspIle: 3.377 ± 0.545
4.432AspLys: 4.432 ± 0.641
6.542AspLeu: 6.542 ± 1.562
1.899AspMet: 1.899 ± 0.415
3.693AspAsn: 3.693 ± 0.725
1.266AspPro: 1.266 ± 0.331
1.372AspGln: 1.372 ± 0.364
2.427AspArg: 2.427 ± 0.503
4.115AspSer: 4.115 ± 0.604
2.849AspThr: 2.849 ± 0.592
4.643AspVal: 4.643 ± 0.609
1.266AspTrp: 1.266 ± 0.486
2.216AspTyr: 2.216 ± 0.576
0.0AspXaa: 0.0 ± 0.0
Glu
6.542GluAla: 6.542 ± 0.822
1.899GluCys: 1.899 ± 0.453
3.06GluAsp: 3.06 ± 0.702
4.115GluGlu: 4.115 ± 0.724
1.477GluPhe: 1.477 ± 0.303
2.743GluGly: 2.743 ± 0.609
1.055GluHis: 1.055 ± 0.352
3.06GluIle: 3.06 ± 0.638
4.326GluLys: 4.326 ± 0.965
6.12GluLeu: 6.12 ± 0.827
1.583GluMet: 1.583 ± 0.456
3.377GluAsn: 3.377 ± 0.562
2.216GluPro: 2.216 ± 0.783
2.427GluGln: 2.427 ± 0.704
3.693GluArg: 3.693 ± 0.561
4.432GluSer: 4.432 ± 0.748
2.638GluThr: 2.638 ± 0.414
3.482GluVal: 3.482 ± 0.55
2.321GluTrp: 2.321 ± 0.62
2.849GluTyr: 2.849 ± 0.591
0.0GluXaa: 0.0 ± 0.0
Phe
2.955PheAla: 2.955 ± 0.735
0.422PheCys: 0.422 ± 0.186
3.482PheAsp: 3.482 ± 0.609
2.005PheGlu: 2.005 ± 0.424
0.844PhePhe: 0.844 ± 0.231
2.849PheGly: 2.849 ± 0.475
0.106PheHis: 0.106 ± 0.127
2.427PheIle: 2.427 ± 0.479
0.95PheLys: 0.95 ± 0.301
1.477PheLeu: 1.477 ± 0.37
1.477PheMet: 1.477 ± 0.433
1.899PheAsn: 1.899 ± 0.544
0.633PhePro: 0.633 ± 0.232
0.633PheGln: 0.633 ± 0.287
1.055PheArg: 1.055 ± 0.322
2.532PheSer: 2.532 ± 0.708
1.688PheThr: 1.688 ± 0.361
1.688PheVal: 1.688 ± 0.353
0.528PheTrp: 0.528 ± 0.22
1.161PheTyr: 1.161 ± 0.431
0.0PheXaa: 0.0 ± 0.0
Gly
6.964GlyAla: 6.964 ± 1.543
1.477GlyCys: 1.477 ± 0.835
4.959GlyAsp: 4.959 ± 1.104
4.537GlyGlu: 4.537 ± 0.874
2.532GlyPhe: 2.532 ± 0.462
4.959GlyGly: 4.959 ± 0.766
0.844GlyHis: 0.844 ± 0.434
5.17GlyIle: 5.17 ± 0.879
5.276GlyLys: 5.276 ± 0.894
4.854GlyLeu: 4.854 ± 0.927
2.11GlyMet: 2.11 ± 0.366
3.588GlyAsn: 3.588 ± 1.298
0.106GlyPro: 0.106 ± 0.098
2.005GlyGln: 2.005 ± 0.52
2.216GlyArg: 2.216 ± 0.431
6.226GlySer: 6.226 ± 1.032
5.065GlyThr: 5.065 ± 1.16
5.487GlyVal: 5.487 ± 0.757
1.899GlyTrp: 1.899 ± 0.615
4.959GlyTyr: 4.959 ± 2.363
0.0GlyXaa: 0.0 ± 0.0
His
1.372HisAla: 1.372 ± 0.415
0.211HisCys: 0.211 ± 0.161
0.95HisAsp: 0.95 ± 0.314
1.266HisGlu: 1.266 ± 0.442
0.317HisPhe: 0.317 ± 0.182
1.055HisGly: 1.055 ± 0.382
0.317HisHis: 0.317 ± 0.179
0.317HisIle: 0.317 ± 0.212
0.528HisLys: 0.528 ± 0.258
0.95HisLeu: 0.95 ± 0.307
0.528HisMet: 0.528 ± 0.235
0.211HisAsn: 0.211 ± 0.17
0.211HisPro: 0.211 ± 0.146
0.317HisGln: 0.317 ± 0.178
0.422HisArg: 0.422 ± 0.245
0.422HisSer: 0.422 ± 0.195
0.422HisThr: 0.422 ± 0.184
1.372HisVal: 1.372 ± 0.455
0.317HisTrp: 0.317 ± 0.202
0.844HisTyr: 0.844 ± 0.275
0.0HisXaa: 0.0 ± 0.0
Ile
5.909IleAla: 5.909 ± 0.872
1.372IleCys: 1.372 ± 0.463
6.12IleAsp: 6.12 ± 0.978
6.437IleGlu: 6.437 ± 0.814
0.739IlePhe: 0.739 ± 0.246
3.166IleGly: 3.166 ± 0.703
0.739IleHis: 0.739 ± 0.306
5.17IleIle: 5.17 ± 0.994
3.377IleLys: 3.377 ± 0.554
2.11IleLeu: 2.11 ± 0.582
1.899IleMet: 1.899 ± 0.506
3.271IleAsn: 3.271 ± 0.544
2.321IlePro: 2.321 ± 0.799
2.427IleGln: 2.427 ± 0.821
2.216IleArg: 2.216 ± 0.586
4.643IleSer: 4.643 ± 0.701
4.432IleThr: 4.432 ± 0.593
3.482IleVal: 3.482 ± 0.696
0.106IleTrp: 0.106 ± 0.112
1.055IleTyr: 1.055 ± 0.275
0.0IleXaa: 0.0 ± 0.0
Lys
7.703LysAla: 7.703 ± 1.318
0.844LysCys: 0.844 ± 0.325
3.06LysAsp: 3.06 ± 0.521
2.321LysGlu: 2.321 ± 0.617
1.477LysPhe: 1.477 ± 0.392
2.743LysGly: 2.743 ± 0.481
1.583LysHis: 1.583 ± 0.567
3.588LysIle: 3.588 ± 0.721
4.221LysLys: 4.221 ± 1.017
4.959LysLeu: 4.959 ± 0.763
1.266LysMet: 1.266 ± 0.398
2.743LysAsn: 2.743 ± 0.515
1.794LysPro: 1.794 ± 0.435
2.321LysGln: 2.321 ± 0.46
3.166LysArg: 3.166 ± 0.734
4.748LysSer: 4.748 ± 0.649
3.588LysThr: 3.588 ± 0.592
3.588LysVal: 3.588 ± 0.652
1.055LysTrp: 1.055 ± 0.562
2.11LysTyr: 2.11 ± 0.485
0.0LysXaa: 0.0 ± 0.0
Leu
7.914LeuAla: 7.914 ± 0.63
1.055LeuCys: 1.055 ± 0.328
5.487LeuAsp: 5.487 ± 0.761
3.799LeuGlu: 3.799 ± 0.605
2.005LeuPhe: 2.005 ± 0.602
5.17LeuGly: 5.17 ± 1.076
1.055LeuHis: 1.055 ± 0.348
3.06LeuIle: 3.06 ± 0.857
4.854LeuLys: 4.854 ± 0.865
3.799LeuLeu: 3.799 ± 0.752
1.688LeuMet: 1.688 ± 0.51
2.743LeuAsn: 2.743 ± 0.559
3.166LeuPro: 3.166 ± 0.916
2.849LeuGln: 2.849 ± 0.49
3.377LeuArg: 3.377 ± 1.581
5.065LeuSer: 5.065 ± 0.964
4.537LeuThr: 4.537 ± 0.565
4.326LeuVal: 4.326 ± 0.615
0.95LeuTrp: 0.95 ± 0.414
2.216LeuTyr: 2.216 ± 0.507
0.0LeuXaa: 0.0 ± 0.0
Met
3.06MetAla: 3.06 ± 0.671
0.528MetCys: 0.528 ± 0.215
2.11MetAsp: 2.11 ± 0.551
1.372MetGlu: 1.372 ± 0.447
1.161MetPhe: 1.161 ± 0.391
1.161MetGly: 1.161 ± 0.31
0.422MetHis: 0.422 ± 0.18
1.899MetIle: 1.899 ± 0.455
0.844MetLys: 0.844 ± 0.28
2.11MetLeu: 2.11 ± 0.508
0.844MetMet: 0.844 ± 0.285
0.739MetAsn: 0.739 ± 0.276
1.583MetPro: 1.583 ± 0.413
1.477MetGln: 1.477 ± 0.404
1.688MetArg: 1.688 ± 0.543
2.638MetSer: 2.638 ± 0.496
1.477MetThr: 1.477 ± 0.33
1.161MetVal: 1.161 ± 0.289
0.317MetTrp: 0.317 ± 0.216
0.317MetTyr: 0.317 ± 0.181
0.0MetXaa: 0.0 ± 0.0
Asn
5.592AsnAla: 5.592 ± 0.615
0.95AsnCys: 0.95 ± 0.267
4.01AsnAsp: 4.01 ± 0.601
2.321AsnGlu: 2.321 ± 0.642
0.844AsnPhe: 0.844 ± 0.247
5.381AsnGly: 5.381 ± 1.248
0.106AsnHis: 0.106 ± 0.117
2.11AsnIle: 2.11 ± 0.447
2.638AsnLys: 2.638 ± 0.531
3.904AsnLeu: 3.904 ± 0.965
1.055AsnMet: 1.055 ± 0.316
1.899AsnAsn: 1.899 ± 0.476
1.899AsnPro: 1.899 ± 0.479
1.055AsnGln: 1.055 ± 0.325
2.11AsnArg: 2.11 ± 0.472
1.794AsnSer: 1.794 ± 0.422
2.321AsnThr: 2.321 ± 0.515
3.693AsnVal: 3.693 ± 0.575
0.422AsnTrp: 0.422 ± 0.209
1.899AsnTyr: 1.899 ± 0.453
0.0AsnXaa: 0.0 ± 0.0
Pro
2.638ProAla: 2.638 ± 0.447
0.211ProCys: 0.211 ± 0.134
2.532ProAsp: 2.532 ± 0.551
2.321ProGlu: 2.321 ± 0.414
1.161ProPhe: 1.161 ± 0.32
1.161ProGly: 1.161 ± 0.328
0.633ProHis: 0.633 ± 0.342
1.055ProIle: 1.055 ± 0.333
1.688ProLys: 1.688 ± 0.499
1.055ProLeu: 1.055 ± 0.334
0.528ProMet: 0.528 ± 0.242
2.427ProAsn: 2.427 ± 0.751
0.739ProPro: 0.739 ± 0.237
1.794ProGln: 1.794 ± 0.8
1.583ProArg: 1.583 ± 0.375
2.216ProSer: 2.216 ± 0.416
2.638ProThr: 2.638 ± 0.579
2.321ProVal: 2.321 ± 0.467
0.317ProTrp: 0.317 ± 0.172
1.372ProTyr: 1.372 ± 0.551
0.0ProXaa: 0.0 ± 0.0
Gln
5.065GlnAla: 5.065 ± 0.721
0.211GlnCys: 0.211 ± 0.17
1.583GlnAsp: 1.583 ± 0.418
2.321GlnGlu: 2.321 ± 0.514
0.739GlnPhe: 0.739 ± 0.328
2.955GlnGly: 2.955 ± 0.668
0.106GlnHis: 0.106 ± 0.104
2.427GlnIle: 2.427 ± 0.708
1.266GlnLys: 1.266 ± 0.31
3.588GlnLeu: 3.588 ± 0.839
1.161GlnMet: 1.161 ± 0.465
0.739GlnAsn: 0.739 ± 0.308
1.055GlnPro: 1.055 ± 0.303
1.688GlnGln: 1.688 ± 0.477
1.583GlnArg: 1.583 ± 0.339
1.688GlnSer: 1.688 ± 0.474
2.955GlnThr: 2.955 ± 0.464
1.794GlnVal: 1.794 ± 0.491
0.211GlnTrp: 0.211 ± 0.156
1.583GlnTyr: 1.583 ± 0.445
0.0GlnXaa: 0.0 ± 0.0
Arg
3.06ArgAla: 3.06 ± 0.56
1.055ArgCys: 1.055 ± 0.329
3.271ArgAsp: 3.271 ± 0.686
3.271ArgGlu: 3.271 ± 0.812
2.532ArgPhe: 2.532 ± 0.67
3.06ArgGly: 3.06 ± 0.817
0.739ArgHis: 0.739 ± 0.358
2.321ArgIle: 2.321 ± 0.614
3.06ArgLys: 3.06 ± 0.573
2.638ArgLeu: 2.638 ± 0.467
0.844ArgMet: 0.844 ± 0.331
2.11ArgAsn: 2.11 ± 0.49
1.055ArgPro: 1.055 ± 0.357
1.266ArgGln: 1.266 ± 0.362
2.638ArgArg: 2.638 ± 0.575
3.377ArgSer: 3.377 ± 0.692
2.638ArgThr: 2.638 ± 0.507
2.743ArgVal: 2.743 ± 0.629
0.633ArgTrp: 0.633 ± 0.311
2.216ArgTyr: 2.216 ± 0.683
0.0ArgXaa: 0.0 ± 0.0
Ser
6.542SerAla: 6.542 ± 0.781
1.266SerCys: 1.266 ± 0.423
5.592SerAsp: 5.592 ± 0.769
2.955SerGlu: 2.955 ± 0.628
3.693SerPhe: 3.693 ± 0.654
6.437SerGly: 6.437 ± 1.306
0.528SerHis: 0.528 ± 0.278
5.381SerIle: 5.381 ± 0.615
4.221SerLys: 4.221 ± 0.819
4.643SerLeu: 4.643 ± 0.729
2.532SerMet: 2.532 ± 0.469
2.532SerAsn: 2.532 ± 0.448
1.899SerPro: 1.899 ± 0.454
2.005SerGln: 2.005 ± 0.441
2.427SerArg: 2.427 ± 0.674
3.904SerSer: 3.904 ± 0.814
4.748SerThr: 4.748 ± 0.879
4.432SerVal: 4.432 ± 0.677
0.95SerTrp: 0.95 ± 0.34
2.005SerTyr: 2.005 ± 0.466
0.0SerXaa: 0.0 ± 0.0
Thr
7.281ThrAla: 7.281 ± 1.231
1.055ThrCys: 1.055 ± 0.304
3.588ThrAsp: 3.588 ± 0.827
2.638ThrGlu: 2.638 ± 0.697
2.216ThrPhe: 2.216 ± 0.454
4.432ThrGly: 4.432 ± 1.051
0.633ThrHis: 0.633 ± 0.218
4.115ThrIle: 4.115 ± 0.682
3.588ThrLys: 3.588 ± 0.827
4.537ThrLeu: 4.537 ± 0.873
1.477ThrMet: 1.477 ± 0.433
3.166ThrAsn: 3.166 ± 0.693
1.899ThrPro: 1.899 ± 0.495
1.899ThrGln: 1.899 ± 0.504
2.743ThrArg: 2.743 ± 0.569
4.643ThrSer: 4.643 ± 0.86
4.959ThrThr: 4.959 ± 0.827
5.698ThrVal: 5.698 ± 1.19
1.794ThrTrp: 1.794 ± 0.721
2.005ThrTyr: 2.005 ± 0.561
0.0ThrXaa: 0.0 ± 0.0
Val
6.331ValAla: 6.331 ± 0.997
0.739ValCys: 0.739 ± 0.25
4.748ValAsp: 4.748 ± 0.677
5.592ValGlu: 5.592 ± 0.698
2.321ValPhe: 2.321 ± 0.66
3.904ValGly: 3.904 ± 0.893
0.422ValHis: 0.422 ± 0.226
4.01ValIle: 4.01 ± 0.807
3.588ValLys: 3.588 ± 0.62
3.166ValLeu: 3.166 ± 0.519
0.95ValMet: 0.95 ± 0.321
2.955ValAsn: 2.955 ± 0.516
3.482ValPro: 3.482 ± 0.674
2.321ValGln: 2.321 ± 0.524
3.693ValArg: 3.693 ± 0.666
3.799ValSer: 3.799 ± 0.474
5.276ValThr: 5.276 ± 0.936
4.115ValVal: 4.115 ± 0.662
0.739ValTrp: 0.739 ± 0.259
2.427ValTyr: 2.427 ± 0.51
0.0ValXaa: 0.0 ± 0.0
Trp
1.161TrpAla: 1.161 ± 0.454
0.422TrpCys: 0.422 ± 0.24
0.633TrpAsp: 0.633 ± 0.247
0.95TrpGlu: 0.95 ± 0.309
0.95TrpPhe: 0.95 ± 0.313
1.372TrpGly: 1.372 ± 0.394
0.317TrpHis: 0.317 ± 0.204
0.633TrpIle: 0.633 ± 0.282
0.633TrpLys: 0.633 ± 0.251
1.372TrpLeu: 1.372 ± 0.507
0.633TrpMet: 0.633 ± 0.257
0.317TrpAsn: 0.317 ± 0.149
0.739TrpPro: 0.739 ± 0.312
0.95TrpGln: 0.95 ± 0.349
0.739TrpArg: 0.739 ± 0.267
1.266TrpSer: 1.266 ± 0.42
0.95TrpThr: 0.95 ± 0.359
0.739TrpVal: 0.739 ± 0.331
0.106TrpTrp: 0.106 ± 0.116
1.161TrpTyr: 1.161 ± 0.955
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.588TyrAla: 3.588 ± 0.602
0.739TyrCys: 0.739 ± 0.257
2.743TyrAsp: 2.743 ± 0.391
2.321TyrGlu: 2.321 ± 0.582
0.317TyrPhe: 0.317 ± 0.181
4.959TyrGly: 4.959 ± 2.355
0.0TyrHis: 0.0 ± 0.0
2.005TyrIle: 2.005 ± 0.652
2.11TyrLys: 2.11 ± 0.406
1.899TyrLeu: 1.899 ± 0.53
0.95TyrMet: 0.95 ± 0.342
1.688TyrAsn: 1.688 ± 0.399
0.844TyrPro: 0.844 ± 0.369
1.055TyrGln: 1.055 ± 0.337
2.955TyrArg: 2.955 ± 0.563
2.638TyrSer: 2.638 ± 0.448
3.06TyrThr: 3.06 ± 0.494
2.955TyrVal: 2.955 ± 0.661
0.211TyrTrp: 0.211 ± 0.155
1.794TyrTyr: 1.794 ± 0.406
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 44 proteins (9478 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski