Klebsiella phage KPN1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; unclassified Tevenvirinae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 153 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9N9G9|A0A5B9N9G9_9CAUD Uncharacterized protein OS=Klebsiella phage KPN1 OX=2601620 GN=KPN1_116 PE=4 SV=1
MM1 pKa = 7.18AQVTVEE7 pKa = 4.24TYY9 pKa = 10.26DD10 pKa = 3.4YY11 pKa = 10.42EE12 pKa = 4.48HH13 pKa = 7.17FIEE16 pKa = 6.11IIEE19 pKa = 4.28KK20 pKa = 9.77YY21 pKa = 10.86GLIEE25 pKa = 4.11VSNKK29 pKa = 8.69SAPWGGNEE37 pKa = 3.68ITVEE41 pKa = 4.02GDD43 pKa = 3.78TPTLWLWLEE52 pKa = 3.9QEE54 pKa = 4.78YY55 pKa = 10.26FPGMDD60 pKa = 3.67DD61 pKa = 3.43EE62 pKa = 4.82CRR64 pKa = 11.84EE65 pKa = 4.14DD66 pKa = 3.48TLTTFSEE73 pKa = 4.55

Molecular weight: 8.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9N9P8|A0A5B9N9P8_9CAUD Uncharacterized protein OS=Klebsiella phage KPN1 OX=2601620 GN=KPN1_53 PE=4 SV=1
MM1 pKa = 7.54GARR4 pKa = 11.84SSEE7 pKa = 4.18VQLLSTVLSVVAPASPQGVRR27 pKa = 11.84LNSYY31 pKa = 10.15LLHH34 pKa = 5.65TVSLKK39 pKa = 10.56VPGPISRR46 pKa = 11.84GG47 pKa = 3.41

Molecular weight: 4.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

153

0

153

30370

29

1032

198.5

22.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.264 ± 0.261

0.971 ± 0.076

6.431 ± 0.142

7.218 ± 0.184

4.241 ± 0.148

6.345 ± 0.223

1.679 ± 0.106

6.704 ± 0.164

7.293 ± 0.221

7.323 ± 0.167

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.878 ± 0.098

5.288 ± 0.158

3.671 ± 0.117

3.461 ± 0.134

4.544 ± 0.123

6.283 ± 0.186

5.815 ± 0.279

6.836 ± 0.183

1.538 ± 0.077

4.218 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski