Cryphonectria parasitica mitovirus 1 (strain American chestnut tree/New Jersey/NB631) (CMV1) (Cryphonectria mitovirus 1)
Average proteome isoelectric point is 9.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q33339|RDRP_CPMV1 RNA-directed RNA polymerase OS=Cryphonectria parasitica mitovirus 1 (strain American chestnut tree/New Jersey/NB631) OX=186769 PE=3 SV=1
MM1 pKa = 8.21 AMIIHH6 pKa = 6.88 DD7 pKa = 4.35 PVYY10 pKa = 10.47 KK11 pKa = 10.48 LWYY14 pKa = 9.18 AXRR17 pKa = 11.84 AKK19 pKa = 10.89 SNLPGLAPHH28 pKa = 6.23 KK29 pKa = 8.95 TEE31 pKa = 5.34 SITTVLSLTNVKK43 pKa = 10.1 NKK45 pKa = 9.81 QATNSILLNKK55 pKa = 8.87 ITDD58 pKa = 3.71 LVLGLKK64 pKa = 10.32 AFSIKK69 pKa = 10.54 SKK71 pKa = 10.12 GVRR74 pKa = 11.84 TTLRR78 pKa = 11.84 VNKK81 pKa = 9.91 HH82 pKa = 5.47 WITPKK87 pKa = 9.94 EE88 pKa = 3.85 FPRR91 pKa = 11.84 FVKK94 pKa = 10.51 LVVWCTRR101 pKa = 11.84 TQEE104 pKa = 4.43 HH105 pKa = 7.23 EE106 pKa = 4.19 DD107 pKa = 3.41 SFMKK111 pKa = 10.53 IIGKK115 pKa = 9.34 CDD117 pKa = 4.66 HH118 pKa = 6.88 IWQTAGPNFLFKK130 pKa = 10.73 YY131 pKa = 9.13 LKK133 pKa = 9.98 EE134 pKa = 4.08 VMRR137 pKa = 11.84 LSVRR141 pKa = 11.84 RR142 pKa = 11.84 IANIEE147 pKa = 4.0 LEE149 pKa = 4.12 PSKK152 pKa = 11.06 KK153 pKa = 10.23 IFVKK157 pKa = 10.39 LNKK160 pKa = 9.32 FRR162 pKa = 11.84 FPNIIPLPICDD173 pKa = 4.86 QIIRR177 pKa = 11.84 DD178 pKa = 3.83 QNDD181 pKa = 2.97 QVLWASKK188 pKa = 10.63 RR189 pKa = 11.84 LIICLLTILSVHH201 pKa = 6.19 RR202 pKa = 11.84 VLPTKK207 pKa = 10.18 VVPDD211 pKa = 3.47 YY212 pKa = 10.86 STIVDD217 pKa = 4.14 PFTGVSKK224 pKa = 9.81 TIDD227 pKa = 3.09 QKK229 pKa = 11.21 LLRR232 pKa = 11.84 KK233 pKa = 9.82 AIHH236 pKa = 6.42 LLNIKK241 pKa = 9.6 RR242 pKa = 11.84 VKK244 pKa = 9.5 QLKK247 pKa = 10.31 LKK249 pKa = 9.18 ITGSMKK255 pKa = 10.45 AGPNGKK261 pKa = 9.21 ISLLTSSVDD270 pKa = 3.1 ALSFITQPTKK280 pKa = 10.46 IFTYY284 pKa = 10.08 LDD286 pKa = 3.36 FSVRR290 pKa = 11.84 VYY292 pKa = 11.03 KK293 pKa = 10.76 FRR295 pKa = 11.84 GLLLWMWMMCILLITLPYY313 pKa = 10.25 AIVSFMLGALIPIMGKK329 pKa = 10.33 LSVVYY334 pKa = 10.13 DD335 pKa = 3.57 QAGKK339 pKa = 10.67 ARR341 pKa = 11.84 IVAITNSWIQTAFYY355 pKa = 10.5 SLHH358 pKa = 5.63 LHH360 pKa = 5.62 VFKK363 pKa = 10.89 LLKK366 pKa = 10.92 NIDD369 pKa = 3.34 QDD371 pKa = 3.76 GTFDD375 pKa = 3.45 QEE377 pKa = 4.53 RR378 pKa = 11.84 PFKK381 pKa = 10.96 LLIKK385 pKa = 9.25 WLNEE389 pKa = 3.66 PTQKK393 pKa = 10.58 FYY395 pKa = 11.47 GFDD398 pKa = 3.07 LTAATDD404 pKa = 3.71 RR405 pKa = 11.84 LPIDD409 pKa = 3.86 LQVDD413 pKa = 3.68 ILNIIFKK420 pKa = 10.32 NSPGSSWRR428 pKa = 11.84 SLLRR432 pKa = 11.84 IKK434 pKa = 10.69 YY435 pKa = 9.48 KK436 pKa = 10.62 SPQGFLTYY444 pKa = 10.76 AVGQPMGAYY453 pKa = 10.13 SSFAMLALTHH463 pKa = 6.12 HH464 pKa = 6.86 VIVQVAALNSGFTTRR479 pKa = 11.84 FTDD482 pKa = 3.65 YY483 pKa = 10.87 CILGDD488 pKa = 5.09 DD489 pKa = 4.01 IVIAHH494 pKa = 6.64 DD495 pKa = 4.06 TVASEE500 pKa = 3.98 YY501 pKa = 10.49 LKK503 pKa = 11.23 LMEE506 pKa = 4.54 TLGLSISSGKK516 pKa = 10.24 SVISSEE522 pKa = 3.82 FTEE525 pKa = 4.71 FAKK528 pKa = 10.72 KK529 pKa = 10.55 LKK531 pKa = 10.04 GRR533 pKa = 11.84 NNFDD537 pKa = 2.43 IFYY540 pKa = 9.55 RR541 pKa = 11.84 SWFSIIHH548 pKa = 5.45 FEE550 pKa = 4.22 KK551 pKa = 10.67 QILHH555 pKa = 6.65 LCTVFEE561 pKa = 4.17 LLRR564 pKa = 11.84 RR565 pKa = 11.84 GVCEE569 pKa = 5.59 LYY571 pKa = 10.66 DD572 pKa = 5.57 LYY574 pKa = 10.56 PQYY577 pKa = 10.96 INKK580 pKa = 9.48 LPKK583 pKa = 9.66 IYY585 pKa = 10.46 LRR587 pKa = 11.84 YY588 pKa = 9.94 NLLIDD593 pKa = 3.56 WVVVAFTNQILIGDD607 pKa = 4.52 RR608 pKa = 11.84 PRR610 pKa = 11.84 ADD612 pKa = 4.34 GIRR615 pKa = 11.84 LFDD618 pKa = 3.64 YY619 pKa = 10.59 FVGLEE624 pKa = 4.2 VIPPLLRR631 pKa = 11.84 IMLHH635 pKa = 5.86 TIKK638 pKa = 10.56 KK639 pKa = 9.61 DD640 pKa = 3.37 WNGLWNSIKK649 pKa = 9.69 YY650 pKa = 7.53 TLNKK654 pKa = 9.72 GFVVSQVRR662 pKa = 11.84 VGLPDD667 pKa = 3.09 WTEE670 pKa = 3.92 LFLLPILPSTYY681 pKa = 9.9 IIIRR685 pKa = 11.84 DD686 pKa = 3.6 YY687 pKa = 11.19 CRR689 pKa = 11.84 SFNDD693 pKa = 3.28 LTKK696 pKa = 11.03 LFGEE700 pKa = 4.17 WWLLRR705 pKa = 11.84 FEE707 pKa = 4.33 SEE709 pKa = 4.45 SYY711 pKa = 10.06 QVSILDD717 pKa = 3.8 VIDD720 pKa = 4.45 RR721 pKa = 11.84 LAHH724 pKa = 5.15 TSIPNLDD731 pKa = 3.02 IHH733 pKa = 6.99 DD734 pKa = 4.12 KK735 pKa = 11.14 KK736 pKa = 10.84 KK737 pKa = 11.27 VKK739 pKa = 9.15 LTLDD743 pKa = 3.43 NLYY746 pKa = 10.72 KK747 pKa = 10.8 LSLIVNIPSGGARR760 pKa = 11.84 RR761 pKa = 11.84 YY762 pKa = 10.1 IEE764 pKa = 3.73 FLRR767 pKa = 11.84 FNGLKK772 pKa = 10.34 SPLIVEE778 pKa = 5.18 RR779 pKa = 11.84 YY780 pKa = 8.4 IKK782 pKa = 10.64 DD783 pKa = 3.74 GIRR786 pKa = 11.84 IEE788 pKa = 5.0 KK789 pKa = 10.0 PLTLQGLHH797 pKa = 6.77 RR798 pKa = 11.84 SGDD801 pKa = 3.28 IQLGFKK807 pKa = 10.36 ISS809 pKa = 3.13
Molecular weight: 93.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.076
IPC2_protein 9.151
IPC_protein 9.048
Toseland 9.853
ProMoST 9.531
Dawson 10.072
Bjellqvist 9.736
Wikipedia 10.218
Rodwell 10.54
Grimsley 10.131
Solomon 10.087
Lehninger 10.043
Nozaki 9.882
DTASelect 9.721
Thurlkill 9.926
EMBOSS 10.262
Sillero 9.984
Patrickios 9.78
IPC_peptide 10.087
IPC2_peptide 8.302
IPC2.peptide.svr19 8.057
Protein with the highest isoelectric point:
>sp|Q33339|RDRP_CPMV1 RNA-directed RNA polymerase OS=Cryphonectria parasitica mitovirus 1 (strain American chestnut tree/New Jersey/NB631) OX=186769 PE=3 SV=1
MM1 pKa = 8.21 AMIIHH6 pKa = 6.88 DD7 pKa = 4.35 PVYY10 pKa = 10.47 KK11 pKa = 10.48 LWYY14 pKa = 9.18 AXRR17 pKa = 11.84 AKK19 pKa = 10.89 SNLPGLAPHH28 pKa = 6.23 KK29 pKa = 8.95 TEE31 pKa = 5.34 SITTVLSLTNVKK43 pKa = 10.1 NKK45 pKa = 9.81 QATNSILLNKK55 pKa = 8.87 ITDD58 pKa = 3.71 LVLGLKK64 pKa = 10.32 AFSIKK69 pKa = 10.54 SKK71 pKa = 10.12 GVRR74 pKa = 11.84 TTLRR78 pKa = 11.84 VNKK81 pKa = 9.91 HH82 pKa = 5.47 WITPKK87 pKa = 9.94 EE88 pKa = 3.85 FPRR91 pKa = 11.84 FVKK94 pKa = 10.51 LVVWCTRR101 pKa = 11.84 TQEE104 pKa = 4.43 HH105 pKa = 7.23 EE106 pKa = 4.19 DD107 pKa = 3.41 SFMKK111 pKa = 10.53 IIGKK115 pKa = 9.34 CDD117 pKa = 4.66 HH118 pKa = 6.88 IWQTAGPNFLFKK130 pKa = 10.73 YY131 pKa = 9.13 LKK133 pKa = 9.98 EE134 pKa = 4.08 VMRR137 pKa = 11.84 LSVRR141 pKa = 11.84 RR142 pKa = 11.84 IANIEE147 pKa = 4.0 LEE149 pKa = 4.12 PSKK152 pKa = 11.06 KK153 pKa = 10.23 IFVKK157 pKa = 10.39 LNKK160 pKa = 9.32 FRR162 pKa = 11.84 FPNIIPLPICDD173 pKa = 4.86 QIIRR177 pKa = 11.84 DD178 pKa = 3.83 QNDD181 pKa = 2.97 QVLWASKK188 pKa = 10.63 RR189 pKa = 11.84 LIICLLTILSVHH201 pKa = 6.19 RR202 pKa = 11.84 VLPTKK207 pKa = 10.18 VVPDD211 pKa = 3.47 YY212 pKa = 10.86 STIVDD217 pKa = 4.14 PFTGVSKK224 pKa = 9.81 TIDD227 pKa = 3.09 QKK229 pKa = 11.21 LLRR232 pKa = 11.84 KK233 pKa = 9.82 AIHH236 pKa = 6.42 LLNIKK241 pKa = 9.6 RR242 pKa = 11.84 VKK244 pKa = 9.5 QLKK247 pKa = 10.31 LKK249 pKa = 9.18 ITGSMKK255 pKa = 10.45 AGPNGKK261 pKa = 9.21 ISLLTSSVDD270 pKa = 3.1 ALSFITQPTKK280 pKa = 10.46 IFTYY284 pKa = 10.08 LDD286 pKa = 3.36 FSVRR290 pKa = 11.84 VYY292 pKa = 11.03 KK293 pKa = 10.76 FRR295 pKa = 11.84 GLLLWMWMMCILLITLPYY313 pKa = 10.25 AIVSFMLGALIPIMGKK329 pKa = 10.33 LSVVYY334 pKa = 10.13 DD335 pKa = 3.57 QAGKK339 pKa = 10.67 ARR341 pKa = 11.84 IVAITNSWIQTAFYY355 pKa = 10.5 SLHH358 pKa = 5.63 LHH360 pKa = 5.62 VFKK363 pKa = 10.89 LLKK366 pKa = 10.92 NIDD369 pKa = 3.34 QDD371 pKa = 3.76 GTFDD375 pKa = 3.45 QEE377 pKa = 4.53 RR378 pKa = 11.84 PFKK381 pKa = 10.96 LLIKK385 pKa = 9.25 WLNEE389 pKa = 3.66 PTQKK393 pKa = 10.58 FYY395 pKa = 11.47 GFDD398 pKa = 3.07 LTAATDD404 pKa = 3.71 RR405 pKa = 11.84 LPIDD409 pKa = 3.86 LQVDD413 pKa = 3.68 ILNIIFKK420 pKa = 10.32 NSPGSSWRR428 pKa = 11.84 SLLRR432 pKa = 11.84 IKK434 pKa = 10.69 YY435 pKa = 9.48 KK436 pKa = 10.62 SPQGFLTYY444 pKa = 10.76 AVGQPMGAYY453 pKa = 10.13 SSFAMLALTHH463 pKa = 6.12 HH464 pKa = 6.86 VIVQVAALNSGFTTRR479 pKa = 11.84 FTDD482 pKa = 3.65 YY483 pKa = 10.87 CILGDD488 pKa = 5.09 DD489 pKa = 4.01 IVIAHH494 pKa = 6.64 DD495 pKa = 4.06 TVASEE500 pKa = 3.98 YY501 pKa = 10.49 LKK503 pKa = 11.23 LMEE506 pKa = 4.54 TLGLSISSGKK516 pKa = 10.24 SVISSEE522 pKa = 3.82 FTEE525 pKa = 4.71 FAKK528 pKa = 10.72 KK529 pKa = 10.55 LKK531 pKa = 10.04 GRR533 pKa = 11.84 NNFDD537 pKa = 2.43 IFYY540 pKa = 9.55 RR541 pKa = 11.84 SWFSIIHH548 pKa = 5.45 FEE550 pKa = 4.22 KK551 pKa = 10.67 QILHH555 pKa = 6.65 LCTVFEE561 pKa = 4.17 LLRR564 pKa = 11.84 RR565 pKa = 11.84 GVCEE569 pKa = 5.59 LYY571 pKa = 10.66 DD572 pKa = 5.57 LYY574 pKa = 10.56 PQYY577 pKa = 10.96 INKK580 pKa = 9.48 LPKK583 pKa = 9.66 IYY585 pKa = 10.46 LRR587 pKa = 11.84 YY588 pKa = 9.94 NLLIDD593 pKa = 3.56 WVVVAFTNQILIGDD607 pKa = 4.52 RR608 pKa = 11.84 PRR610 pKa = 11.84 ADD612 pKa = 4.34 GIRR615 pKa = 11.84 LFDD618 pKa = 3.64 YY619 pKa = 10.59 FVGLEE624 pKa = 4.2 VIPPLLRR631 pKa = 11.84 IMLHH635 pKa = 5.86 TIKK638 pKa = 10.56 KK639 pKa = 9.61 DD640 pKa = 3.37 WNGLWNSIKK649 pKa = 9.69 YY650 pKa = 7.53 TLNKK654 pKa = 9.72 GFVVSQVRR662 pKa = 11.84 VGLPDD667 pKa = 3.09 WTEE670 pKa = 3.92 LFLLPILPSTYY681 pKa = 9.9 IIIRR685 pKa = 11.84 DD686 pKa = 3.6 YY687 pKa = 11.19 CRR689 pKa = 11.84 SFNDD693 pKa = 3.28 LTKK696 pKa = 11.03 LFGEE700 pKa = 4.17 WWLLRR705 pKa = 11.84 FEE707 pKa = 4.33 SEE709 pKa = 4.45 SYY711 pKa = 10.06 QVSILDD717 pKa = 3.8 VIDD720 pKa = 4.45 RR721 pKa = 11.84 LAHH724 pKa = 5.15 TSIPNLDD731 pKa = 3.02 IHH733 pKa = 6.99 DD734 pKa = 4.12 KK735 pKa = 11.14 KK736 pKa = 10.84 KK737 pKa = 11.27 VKK739 pKa = 9.15 LTLDD743 pKa = 3.43 NLYY746 pKa = 10.72 KK747 pKa = 10.8 LSLIVNIPSGGARR760 pKa = 11.84 RR761 pKa = 11.84 YY762 pKa = 10.1 IEE764 pKa = 3.73 FLRR767 pKa = 11.84 FNGLKK772 pKa = 10.34 SPLIVEE778 pKa = 5.18 RR779 pKa = 11.84 YY780 pKa = 8.4 IKK782 pKa = 10.64 DD783 pKa = 3.74 GIRR786 pKa = 11.84 IEE788 pKa = 5.0 KK789 pKa = 10.0 PLTLQGLHH797 pKa = 6.77 RR798 pKa = 11.84 SGDD801 pKa = 3.28 IQLGFKK807 pKa = 10.36 ISS809 pKa = 3.13
Molecular weight: 93.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.076
IPC2_protein 9.151
IPC_protein 9.048
Toseland 9.853
ProMoST 9.531
Dawson 10.072
Bjellqvist 9.736
Wikipedia 10.218
Rodwell 10.54
Grimsley 10.131
Solomon 10.087
Lehninger 10.043
Nozaki 9.882
DTASelect 9.721
Thurlkill 9.926
EMBOSS 10.262
Sillero 9.984
Patrickios 9.78
IPC_peptide 10.087
IPC2_peptide 8.302
IPC2.peptide.svr19 8.057
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
809
809
809
809.0
93.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.079 ± 0.0
1.112 ± 0.0
5.068 ± 0.0
2.967 ± 0.0
5.439 ± 0.0
4.821 ± 0.0
2.225 ± 0.0
10.012 ± 0.0
8.035 ± 0.0
13.35 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.731 ± 0.0
3.956 ± 0.0
4.326 ± 0.0
3.09 ± 0.0
5.192 ± 0.0
6.551 ± 0.0
5.81 ± 0.0
6.428 ± 0.0
2.101 ± 0.0
3.585 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here