Peptoniphilus sp. BV3C26

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Peptoniphilaceae; Peptoniphilus; unclassified Peptoniphilus

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1924 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U7UC12|U7UC12_9FIRM Sigma-70 region 2 OS=Peptoniphilus sp. BV3C26 OX=1111134 GN=HMPREF1253_1044 PE=3 SV=1
MM1 pKa = 7.1YY2 pKa = 8.96PSLFVVEE9 pKa = 4.22VEE11 pKa = 4.49NGIEE15 pKa = 4.17MTTSSYY21 pKa = 10.76TYY23 pKa = 10.98SDD25 pKa = 3.73VLTSTVMISVIEE37 pKa = 4.11DD38 pKa = 4.41DD39 pKa = 4.62IDD41 pKa = 4.04LEE43 pKa = 4.3EE44 pKa = 4.1YY45 pKa = 10.37RR46 pKa = 11.84EE47 pKa = 4.35KK48 pKa = 10.71ISS50 pKa = 3.61

Molecular weight: 5.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U7UPY1|U7UPY1_9FIRM ABC transporter ATP-binding protein OS=Peptoniphilus sp. BV3C26 OX=1111134 GN=HMPREF1253_0273 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPKK8 pKa = 8.18NKK10 pKa = 8.76QRR12 pKa = 11.84KK13 pKa = 7.85RR14 pKa = 11.84EE15 pKa = 3.9HH16 pKa = 6.14GFRR19 pKa = 11.84ARR21 pKa = 11.84MRR23 pKa = 11.84TKK25 pKa = 10.32SGRR28 pKa = 11.84AVIKK32 pKa = 9.97ARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.35GRR39 pKa = 11.84KK40 pKa = 8.86KK41 pKa = 10.58LSAA44 pKa = 3.95

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1924

0

1924

581713

37

4792

302.3

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.568 ± 0.063

0.86 ± 0.018

5.916 ± 0.055

7.875 ± 0.069

4.789 ± 0.051

6.387 ± 0.057

1.276 ± 0.022

9.368 ± 0.069

9.954 ± 0.076

9.453 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.516 ± 0.032

5.935 ± 0.056

2.838 ± 0.032

2.444 ± 0.028

3.655 ± 0.044

6.128 ± 0.043

4.62 ± 0.054

5.792 ± 0.049

0.622 ± 0.016

4.006 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski