Sutterella sp. CAG:397
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1804 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R7C0J0|R7C0J0_9BURK Ribonuclease HII OS=Sutterella sp. CAG:397 OX=1262976 GN=rnhB PE=3 SV=1
MM1 pKa = 7.73 PEE3 pKa = 3.79 EE4 pKa = 3.98 LVIFVFYY11 pKa = 11.04 DD12 pKa = 3.39 VVGNGGNAIFVEE24 pKa = 4.76 YY25 pKa = 10.53 SLSSQFGLYY34 pKa = 10.28 LVVIEE39 pKa = 4.58 MEE41 pKa = 3.92 EE42 pKa = 4.05 FGYY45 pKa = 10.16 QFQWTHH51 pKa = 4.92
Molecular weight: 5.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.994
IPC2_protein 3.961
IPC_protein 3.605
Toseland 3.516
ProMoST 3.77
Dawson 3.579
Bjellqvist 3.77
Wikipedia 3.503
Rodwell 3.49
Grimsley 3.465
Solomon 3.49
Lehninger 3.439
Nozaki 3.77
DTASelect 3.719
Thurlkill 3.592
EMBOSS 3.516
Sillero 3.732
Patrickios 0.006
IPC_peptide 3.49
IPC2_peptide 3.694
IPC2.peptide.svr19 3.709
Protein with the highest isoelectric point:
>tr|R7C4R1|R7C4R1_9BURK ATP synthase epsilon chain OS=Sutterella sp. CAG:397 OX=1262976 GN=atpC PE=3 SV=1
MM1 pKa = 6.85 ATKK4 pKa = 9.51 RR5 pKa = 11.84 TYY7 pKa = 10.29 QPSKK11 pKa = 8.35 IKK13 pKa = 10.26 RR14 pKa = 11.84 VRR16 pKa = 11.84 THH18 pKa = 6.04 GFLVRR23 pKa = 11.84 SRR25 pKa = 11.84 TKK27 pKa = 10.45 GGRR30 pKa = 11.84 HH31 pKa = 3.49 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 AKK39 pKa = 10.27 GRR41 pKa = 11.84 HH42 pKa = 5.09 KK43 pKa = 10.72 LALL46 pKa = 3.92
Molecular weight: 5.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1804
0
1804
560900
32
1940
310.9
34.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.673 ± 0.076
1.314 ± 0.022
5.278 ± 0.044
6.197 ± 0.061
4.076 ± 0.04
7.683 ± 0.058
1.826 ± 0.026
5.205 ± 0.04
5.299 ± 0.052
9.608 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.666 ± 0.029
3.051 ± 0.028
4.465 ± 0.034
2.829 ± 0.031
6.039 ± 0.05
5.259 ± 0.04
5.905 ± 0.039
7.908 ± 0.054
1.262 ± 0.024
2.454 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here