Chaetoceros protobacilladnavirus 2 (Chaetoceros sp. DNA virus 7)
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|W6JIC6|CAPSD_CPBDV Capsid protein OS=Chaetoceros protobacilladnavirus 2 OX=2170141 PE=3 SV=1
MM1 pKa = 6.99 NVKK4 pKa = 9.96 GASDD8 pKa = 3.66 KK9 pKa = 11.47 AQLAMQAEE17 pKa = 4.67 WEE19 pKa = 4.28 EE20 pKa = 4.07 VLAPEE25 pKa = 4.5 GALAVEE31 pKa = 4.71 EE32 pKa = 4.33 ASSVLKK38 pKa = 10.77 ISDD41 pKa = 3.59 DD42 pKa = 3.49 EE43 pKa = 4.26 RR44 pKa = 11.84 RR45 pKa = 11.84 SYY47 pKa = 10.69 AAYY50 pKa = 9.14 IDD52 pKa = 4.99 NIIQEE57 pKa = 4.14 NEE59 pKa = 3.26 IDD61 pKa = 3.76 VVYY64 pKa = 10.4 GHH66 pKa = 6.37 SRR68 pKa = 11.84 GAAIASEE75 pKa = 4.63 LEE77 pKa = 3.89 SDD79 pKa = 3.98 VQIIGLDD86 pKa = 3.38 GAMVIANDD94 pKa = 3.34 QANFLNIRR102 pKa = 11.84 QDD104 pKa = 3.25 DD105 pKa = 4.15 SEE107 pKa = 5.87 GYY109 pKa = 10.86 GFDD112 pKa = 3.29 RR113 pKa = 11.84 TIAGPYY119 pKa = 8.47 EE120 pKa = 3.68 NTVIVKK126 pKa = 10.05 GGAFHH131 pKa = 7.27 KK132 pKa = 10.57 VAVPEE137 pKa = 5.08 GYY139 pKa = 8.05 HH140 pKa = 4.83 TKK142 pKa = 10.3 KK143 pKa = 10.53 PKK145 pKa = 10.29 ASQEE149 pKa = 3.79 ALEE152 pKa = 3.94 RR153 pKa = 11.84 AKK155 pKa = 10.78 ARR157 pKa = 11.84 KK158 pKa = 9.06 HH159 pKa = 4.43 NRR161 pKa = 11.84 ARR163 pKa = 11.84 HH164 pKa = 4.71 IARR167 pKa = 11.84 AIDD170 pKa = 3.44 RR171 pKa = 11.84 LTARR175 pKa = 11.84 KK176 pKa = 7.59 TDD178 pKa = 3.3 KK179 pKa = 10.65 EE180 pKa = 4.01 LEE182 pKa = 4.08 EE183 pKa = 4.37 IYY185 pKa = 10.04 WRR187 pKa = 11.84 EE188 pKa = 3.36 RR189 pKa = 11.84 RR190 pKa = 11.84 KK191 pKa = 10.35 KK192 pKa = 10.34 NKK194 pKa = 10.03 NEE196 pKa = 3.57 FSKK199 pKa = 11.3 ALIEE203 pKa = 4.94 FIEE206 pKa = 4.29 EE207 pKa = 3.8 QLLL210 pKa = 3.73
Molecular weight: 23.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.411
IPC2_protein 5.423
IPC_protein 5.372
Toseland 5.486
ProMoST 5.563
Dawson 5.423
Bjellqvist 5.525
Wikipedia 5.321
Rodwell 5.372
Grimsley 5.474
Solomon 5.423
Lehninger 5.397
Nozaki 5.588
DTASelect 5.715
Thurlkill 5.512
EMBOSS 5.448
Sillero 5.677
Patrickios 4.482
IPC_peptide 5.436
IPC2_peptide 5.664
IPC2.peptide.svr19 5.73
Protein with the highest isoelectric point:
>sp|W6JIC6|CAPSD_CPBDV Capsid protein OS=Chaetoceros protobacilladnavirus 2 OX=2170141 PE=3 SV=1
MM1 pKa = 7.61 ARR3 pKa = 11.84 TKK5 pKa = 10.62 SKK7 pKa = 9.63 PRR9 pKa = 11.84 KK10 pKa = 7.43 RR11 pKa = 11.84 TTVRR15 pKa = 11.84 KK16 pKa = 9.5 ARR18 pKa = 11.84 RR19 pKa = 11.84 SVKK22 pKa = 10.16 RR23 pKa = 11.84 RR24 pKa = 11.84 TTTKK28 pKa = 7.98 GTKK31 pKa = 9.6 RR32 pKa = 11.84 KK33 pKa = 7.41 TAGDD37 pKa = 3.84 PVTKK41 pKa = 10.23 AKK43 pKa = 10.48 RR44 pKa = 11.84 GVATSSPFAAHH55 pKa = 6.28 HH56 pKa = 5.77 AVRR59 pKa = 11.84 MNPFSGATTQPKK71 pKa = 10.02 IPDD74 pKa = 3.57 GGFTSSLSRR83 pKa = 11.84 RR84 pKa = 11.84 LQNVVEE90 pKa = 4.3 VTNASNEE97 pKa = 4.48 GIMHH101 pKa = 7.19 LVMAPTMGVPICVTHH116 pKa = 4.7 TTEE119 pKa = 4.41 GASTRR124 pKa = 11.84 SGATLKK130 pKa = 10.58 PSYY133 pKa = 10.54 LGFLGQGVGLEE144 pKa = 4.31 TKK146 pKa = 10.48 VGGVIKK152 pKa = 10.2 WPIANTDD159 pKa = 3.22 TGDD162 pKa = 3.71 VINAADD168 pKa = 3.7 FAKK171 pKa = 9.96 WRR173 pKa = 11.84 VVSQGLQITLNNVDD187 pKa = 5.01 DD188 pKa = 5.37 EE189 pKa = 4.62 NDD191 pKa = 3.14 GWFEE195 pKa = 3.72 AVRR198 pKa = 11.84 FNWRR202 pKa = 11.84 NDD204 pKa = 3.37 NEE206 pKa = 4.91 DD207 pKa = 3.14 ICLTSLDD214 pKa = 3.91 GTDD217 pKa = 3.14 TGNVIGAAPNLNGLPLLTANIVEE240 pKa = 4.28 MPGYY244 pKa = 10.47 KK245 pKa = 9.74 SGLLKK250 pKa = 10.47 DD251 pKa = 3.32 IKK253 pKa = 10.68 DD254 pKa = 3.99 YY255 pKa = 11.61 QFMLHH260 pKa = 6.23 PQQTRR265 pKa = 11.84 HH266 pKa = 6.45 DD267 pKa = 3.91 PVEE270 pKa = 3.93 ITKK273 pKa = 10.86 SIDD276 pKa = 3.47 FVGGTDD282 pKa = 4.55 LNYY285 pKa = 9.07 DD286 pKa = 3.93 TQSKK290 pKa = 9.58 KK291 pKa = 11.15 ANLGDD296 pKa = 3.97 SAAGTLLKK304 pKa = 10.59 QGLVDD309 pKa = 5.19 QNMDD313 pKa = 2.33 WMYY316 pKa = 11.18 IRR318 pKa = 11.84 IHH320 pKa = 6.19 PRR322 pKa = 11.84 SNTGAAGQTGSKK334 pKa = 9.82 LICNYY339 pKa = 9.62 IQNLEE344 pKa = 4.22 FAFSPDD350 pKa = 3.21 SDD352 pKa = 4.04 LATYY356 pKa = 7.5 MTTNRR361 pKa = 11.84 MDD363 pKa = 3.93 PKK365 pKa = 10.22 SAKK368 pKa = 10.38 INDD371 pKa = 3.8 QLNNNQDD378 pKa = 3.23 AANKK382 pKa = 9.55 KK383 pKa = 10.34 RR384 pKa = 11.84 EE385 pKa = 4.1 FNN387 pKa = 3.6
Molecular weight: 42.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.879
IPC2_protein 8.829
IPC_protein 8.741
Toseland 9.706
ProMoST 9.311
Dawson 9.882
Bjellqvist 9.531
Wikipedia 10.014
Rodwell 10.365
Grimsley 9.926
Solomon 9.911
Lehninger 9.897
Nozaki 9.75
DTASelect 9.502
Thurlkill 9.75
EMBOSS 10.101
Sillero 9.809
Patrickios 9.823
IPC_peptide 9.911
IPC2_peptide 8.009
IPC2.peptide.svr19 7.795
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1081
210
484
360.3
40.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.048 ± 1.78
1.11 ± 0.463
6.846 ± 0.252
7.216 ± 2.139
4.07 ± 0.874
6.846 ± 0.79
3.053 ± 0.737
5.365 ± 0.861
7.216 ± 0.145
5.828 ± 0.631
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.758 ± 0.514
5.92 ± 0.743
4.718 ± 0.964
4.07 ± 0.153
5.92 ± 0.387
5.643 ± 0.163
6.383 ± 1.764
5.273 ± 0.538
1.943 ± 0.546
2.775 ± 0.452
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here