Dragonfly larvae associated circular virus-9
Average proteome isoelectric point is 8.14
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W5U284|W5U284_9VIRU Putative capsid protein OS=Dragonfly larvae associated circular virus-9 OX=1454030 PE=4 SV=1
MM1 pKa = 7.24 SARR4 pKa = 11.84 VSFKK8 pKa = 10.37 FSLIKK13 pKa = 9.95 TSATCCSSSIPKK25 pKa = 9.24 MGHH28 pKa = 4.75 QVKK31 pKa = 10.07 RR32 pKa = 11.84 WCFTLNNYY40 pKa = 7.52 TEE42 pKa = 4.29 EE43 pKa = 4.34 EE44 pKa = 4.11 YY45 pKa = 11.19 KK46 pKa = 10.92 EE47 pKa = 3.88 IVEE50 pKa = 4.45 KK51 pKa = 10.94 IEE53 pKa = 3.41 WTYY56 pKa = 10.91 IIIGRR61 pKa = 11.84 EE62 pKa = 3.91 VGEE65 pKa = 4.49 EE66 pKa = 4.23 GTPHH70 pKa = 5.48 LQGYY74 pKa = 9.57 IEE76 pKa = 4.51 LKK78 pKa = 10.49 KK79 pKa = 10.67 KK80 pKa = 10.85 SSLNQIKK87 pKa = 10.18 KK88 pKa = 9.85 VNQKK92 pKa = 9.13 IHH94 pKa = 6.08 WEE96 pKa = 3.91 QLRR99 pKa = 11.84 GKK101 pKa = 8.62 PFQAADD107 pKa = 3.52 YY108 pKa = 9.96 CKK110 pKa = 10.57 KK111 pKa = 10.57 EE112 pKa = 4.1 NNFQEE117 pKa = 4.15 WGHH120 pKa = 7.76 ISMSGGGTTEE130 pKa = 4.14 KK131 pKa = 10.17 IGAIIEE137 pKa = 4.28 KK138 pKa = 9.87 AKK140 pKa = 10.45 SGGMRR145 pKa = 11.84 EE146 pKa = 4.17 VLKK149 pKa = 10.72 DD150 pKa = 3.65 PPKK153 pKa = 10.37 LATVRR158 pKa = 11.84 LIEE161 pKa = 4.98 KK162 pKa = 9.39 YY163 pKa = 10.42 LSYY166 pKa = 11.04 CEE168 pKa = 4.38 EE169 pKa = 3.83 EE170 pKa = 4.67 RR171 pKa = 11.84 KK172 pKa = 9.86 EE173 pKa = 3.93 KK174 pKa = 10.52 PYY176 pKa = 10.77 VHH178 pKa = 7.1 WNLGNITTYY187 pKa = 9.07 TGRR190 pKa = 11.84 MTPNGGDD197 pKa = 3.46 GYY199 pKa = 11.02 DD200 pKa = 3.43 KK201 pKa = 11.55 NEE203 pKa = 4.5 TIVIDD208 pKa = 4.11 DD209 pKa = 4.06 FRR211 pKa = 11.84 ASIMRR216 pKa = 11.84 FTYY219 pKa = 10.71 LLRR222 pKa = 11.84 ILDD225 pKa = 4.64 RR226 pKa = 11.84 YY227 pKa = 8.78 PMRR230 pKa = 11.84 VEE232 pKa = 3.94 VKK234 pKa = 10.23 GGYY237 pKa = 8.97 RR238 pKa = 11.84 QLNSKK243 pKa = 10.51 NIVITSIIHH252 pKa = 6.41 PEE254 pKa = 3.87 SCYY257 pKa = 10.82 HH258 pKa = 6.11 MEE260 pKa = 4.33 NEE262 pKa = 5.26 PIRR265 pKa = 11.84 QLMRR269 pKa = 11.84 RR270 pKa = 11.84 IDD272 pKa = 4.9 KK273 pKa = 9.14 ITEE276 pKa = 4.16 TNNKK280 pKa = 9.81 NILNDD285 pKa = 3.31 VMLQATSEE293 pKa = 4.17 EE294 pKa = 4.86 SVGNTNLHH302 pKa = 6.55 FLICDD307 pKa = 3.65 EE308 pKa = 4.62 EE309 pKa = 5.21 LGEE312 pKa = 4.19
Molecular weight: 36.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.031
IPC2_protein 7.424
IPC_protein 7.337
Toseland 7.059
ProMoST 7.805
Dawson 8.039
Bjellqvist 8.317
Wikipedia 7.951
Rodwell 8.053
Grimsley 7.117
Solomon 8.112
Lehninger 8.141
Nozaki 8.521
DTASelect 8.126
Thurlkill 8.185
EMBOSS 8.258
Sillero 8.492
Patrickios 4.342
IPC_peptide 8.112
IPC2_peptide 7.322
IPC2.peptide.svr19 7.516
Protein with the highest isoelectric point:
>tr|W5U284|W5U284_9VIRU Putative capsid protein OS=Dragonfly larvae associated circular virus-9 OX=1454030 PE=4 SV=1
MM1 pKa = 6.93 SHH3 pKa = 5.58 FRR5 pKa = 11.84 YY6 pKa = 9.54 RR7 pKa = 11.84 RR8 pKa = 11.84 RR9 pKa = 11.84 ATRR12 pKa = 11.84 RR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 FYY17 pKa = 9.97 KK18 pKa = 10.39 RR19 pKa = 11.84 KK20 pKa = 7.96 FKK22 pKa = 10.7 RR23 pKa = 11.84 YY24 pKa = 5.45 TRR26 pKa = 11.84 RR27 pKa = 11.84 HH28 pKa = 4.46 ARR30 pKa = 11.84 RR31 pKa = 11.84 PLSRR35 pKa = 11.84 PYY37 pKa = 10.0 RR38 pKa = 11.84 RR39 pKa = 11.84 AFFKK43 pKa = 10.73 CRR45 pKa = 11.84 ASYY48 pKa = 9.46 EE49 pKa = 4.45 VKK51 pKa = 10.58 GSNGPAFVAYY61 pKa = 9.16 TDD63 pKa = 3.6 NPVNIGDD70 pKa = 3.97 FNSIQNLYY78 pKa = 8.77 AQYY81 pKa = 10.39 RR82 pKa = 11.84 VAGMQLRR89 pKa = 11.84 WIPSFNKK96 pKa = 9.8 NDD98 pKa = 4.22 MIPVEE103 pKa = 4.03 QGEE106 pKa = 4.28 QLGEE110 pKa = 3.82 WLLIKK115 pKa = 10.36 QDD117 pKa = 4.45 DD118 pKa = 4.25 ALTDD122 pKa = 3.43 KK123 pKa = 11.14 AFPNTVQNYY132 pKa = 8.55 LNRR135 pKa = 11.84 DD136 pKa = 2.98 NGLRR140 pKa = 11.84 RR141 pKa = 11.84 RR142 pKa = 11.84 PLVRR146 pKa = 11.84 SWSIYY151 pKa = 9.83 FKK153 pKa = 9.67 MSKK156 pKa = 9.02 MVPLNVASVNVVNLRR171 pKa = 11.84 GGYY174 pKa = 9.62 ISTVQPTATQTVRR187 pKa = 11.84 AMLSSIPVNAGEE199 pKa = 4.11 VLLGTFHH206 pKa = 5.43 VTWYY210 pKa = 9.59 VSAMQRR216 pKa = 11.84 RR217 pKa = 11.84 XIKK220 pKa = 10.36 YY221 pKa = 9.93 IIYY224 pKa = 9.66 LKK226 pKa = 10.58 KK227 pKa = 10.12 KK228 pKa = 9.7 IIFIVYY234 pKa = 8.5 YY235 pKa = 8.64 INFYY239 pKa = 10.48 IRR241 pKa = 11.84 TLIRR245 pKa = 11.84 GEE247 pKa = 4.06 RR248 pKa = 11.84 EE249 pKa = 3.34 RR250 pKa = 11.84 AAVPGVSRR258 pKa = 11.84 HH259 pKa = 5.07 FLQILRR265 pKa = 11.84 NFFF268 pKa = 3.84
Molecular weight: 31.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.255
IPC2_protein 10.306
IPC_protein 11.242
Toseland 11.184
ProMoST 11.433
Dawson 11.242
Bjellqvist 11.125
Wikipedia 11.608
Rodwell 11.228
Grimsley 11.301
Solomon 11.564
Lehninger 11.491
Nozaki 11.169
DTASelect 11.125
Thurlkill 11.184
EMBOSS 11.637
Sillero 11.213
Patrickios 10.906
IPC_peptide 11.564
IPC2_peptide 10.452
IPC2.peptide.svr19 8.361
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
580
268
312
290.0
33.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.483 ± 1.243
1.379 ± 0.672
2.931 ± 0.213
6.724 ± 2.748
4.138 ± 1.224
6.034 ± 0.791
2.069 ± 0.385
8.103 ± 0.927
7.241 ± 1.348
6.897 ± 0.129
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.931 ± 0.213
6.207 ± 0.091
3.793 ± 0.457
3.621 ± 0.323
8.966 ± 2.736
5.862 ± 0.177
5.517 ± 0.695
6.034 ± 1.204
1.552 ± 0.04
5.345 ± 0.667
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here