Hubei toti-like virus 2
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KEZ6|A0A1L3KEZ6_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 2 OX=1923308 PE=4 SV=1
MM1 pKa = 7.97 DD2 pKa = 4.68 GFVKK6 pKa = 10.32 RR7 pKa = 11.84 FYY9 pKa = 10.07 PEE11 pKa = 3.87 HH12 pKa = 6.33 EE13 pKa = 4.15 APGIEE18 pKa = 4.46 FKK20 pKa = 11.22 SEE22 pKa = 3.45 GDD24 pKa = 3.73 LKK26 pKa = 11.07 FGTYY30 pKa = 9.69 YY31 pKa = 10.99 GGVLRR36 pKa = 11.84 VFDD39 pKa = 3.81 GHH41 pKa = 5.36 VQSSRR46 pKa = 11.84 NHH48 pKa = 5.59 YY49 pKa = 10.06 AVQEE53 pKa = 3.92 MTAAGKK59 pKa = 10.23 LKK61 pKa = 10.26 AVLMGTSSSLDD72 pKa = 3.43 GLTKK76 pKa = 10.36 QYY78 pKa = 8.15 LTPEE82 pKa = 3.94 GMVNISTVAEE92 pKa = 4.04 ALKK95 pKa = 10.62 QSAGLQTQVWSTHH108 pKa = 4.22 VNRR111 pKa = 11.84 MQHH114 pKa = 5.94 WGWADD119 pKa = 3.19 NHH121 pKa = 6.45 VSLLVNLLRR130 pKa = 11.84 FSILLRR136 pKa = 11.84 TQEE139 pKa = 4.39 SNGGNLDD146 pKa = 3.73 CSLGVYY152 pKa = 9.43 MDD154 pKa = 3.03 GHH156 pKa = 7.06 VNVDD160 pKa = 3.09 MDD162 pKa = 3.77 QWWSSEE168 pKa = 3.91 YY169 pKa = 9.65 PEE171 pKa = 4.79 KK172 pKa = 11.14 YY173 pKa = 10.72 KK174 pKa = 10.28 MDD176 pKa = 3.48 TWPTGTGEE184 pKa = 4.11 EE185 pKa = 4.48 NYY187 pKa = 10.15 PEE189 pKa = 4.3 YY190 pKa = 11.37 AHH192 pKa = 6.45 LTSTTPDD199 pKa = 3.36 TDD201 pKa = 3.8 SNAIDD206 pKa = 4.13 LRR208 pKa = 11.84 GLTAVEE214 pKa = 3.81 ANIILMMTGAWSRR227 pKa = 11.84 KK228 pKa = 6.83 SRR230 pKa = 11.84 CRR232 pKa = 11.84 LDD234 pKa = 3.65 FSTPRR239 pKa = 11.84 LTEE242 pKa = 3.63 LLYY245 pKa = 10.44 YY246 pKa = 10.38 RR247 pKa = 11.84 HH248 pKa = 6.57 GSTMTGLEE256 pKa = 3.92 SWLKK260 pKa = 9.51 EE261 pKa = 3.97 EE262 pKa = 5.4 QGAEE266 pKa = 4.1 MPKK269 pKa = 9.34 MPNWKK274 pKa = 9.38 QVWSALRR281 pKa = 11.84 AYY283 pKa = 10.1 VGQNRR288 pKa = 11.84 LYY290 pKa = 10.71 DD291 pKa = 3.63 QFSTALYY298 pKa = 9.21 IVSFLAYY305 pKa = 9.89 QFVPATAEE313 pKa = 3.86 ACVWLSFDD321 pKa = 3.47 WQIVMPVFHH330 pKa = 7.51 SIRR333 pKa = 11.84 GRR335 pKa = 11.84 YY336 pKa = 8.45 EE337 pKa = 3.42 ILNDD341 pKa = 3.64 GEE343 pKa = 4.29 AALVSHH349 pKa = 7.01 RR350 pKa = 11.84 AINEE354 pKa = 3.58 WGFINGKK361 pKa = 8.27 LEE363 pKa = 4.67 KK364 pKa = 10.15 INLMCMLVAQCFQTGCAVRR383 pKa = 11.84 AMRR386 pKa = 11.84 KK387 pKa = 9.51 GLEE390 pKa = 4.11 TDD392 pKa = 3.03 PHH394 pKa = 7.15 DD395 pKa = 4.62 LYY397 pKa = 11.02 STEE400 pKa = 4.14 TEE402 pKa = 4.77 FYY404 pKa = 9.79 SASSFMSAAVSEE416 pKa = 4.54 ATRR419 pKa = 11.84 SEE421 pKa = 4.18 FPMPGMAGIYY431 pKa = 10.13 LRR433 pKa = 11.84 TDD435 pKa = 2.77 VRR437 pKa = 11.84 FDD439 pKa = 3.93 DD440 pKa = 4.39 PGEE443 pKa = 3.97 FQIMTLNTSEE453 pKa = 4.77 RR454 pKa = 11.84 GLEE457 pKa = 4.42 GYY459 pKa = 8.36 DD460 pKa = 3.36 TEE462 pKa = 4.76 VIEE465 pKa = 5.41 SKK467 pKa = 9.81 TKK469 pKa = 10.12 EE470 pKa = 3.61 IKK472 pKa = 10.0 KK473 pKa = 8.42 VHH475 pKa = 6.5 KK476 pKa = 9.93 EE477 pKa = 3.74 LSDD480 pKa = 3.84 LEE482 pKa = 4.65 GKK484 pKa = 9.67 EE485 pKa = 4.24 KK486 pKa = 10.7 YY487 pKa = 10.41 AEE489 pKa = 4.17 ALAEE493 pKa = 3.88 ISMLKK498 pKa = 10.17 QIAEE502 pKa = 4.35 IKK504 pKa = 10.38 LQMKK508 pKa = 10.4 SSTLSYY514 pKa = 10.17 QEE516 pKa = 4.08 KK517 pKa = 9.94 LAVEE521 pKa = 4.06 NKK523 pKa = 9.64 YY524 pKa = 10.58 VVEE527 pKa = 4.39 TEE529 pKa = 4.02 VEE531 pKa = 4.31 TTVNLIAVKK540 pKa = 9.88 CVWVPMAGSPVLALPLNPFPYY561 pKa = 10.35 NSPLTLKK568 pKa = 9.85 GTIEE572 pKa = 3.96 PAMGSLSRR580 pKa = 11.84 KK581 pKa = 7.75 GFKK584 pKa = 10.45 VSADD588 pKa = 3.86 KK589 pKa = 11.08 AWLIANFTRR598 pKa = 11.84 LCGYY602 pKa = 10.35 DD603 pKa = 3.47 VLFKK607 pKa = 10.6 QHH609 pKa = 6.56 GEE611 pKa = 3.82 QAGPSDD617 pKa = 3.94 YY618 pKa = 10.69 FAPNNVNMVWPILATDD634 pKa = 4.54 DD635 pKa = 5.16 DD636 pKa = 4.33 QDD638 pKa = 3.73 EE639 pKa = 5.43 PITITGMEE647 pKa = 3.89 EE648 pKa = 3.68 RR649 pKa = 11.84 DD650 pKa = 3.58 RR651 pKa = 11.84 LFVSLPPIHH660 pKa = 6.78 NKK662 pKa = 9.41 FFSNQKK668 pKa = 10.13 LEE670 pKa = 3.83 YY671 pKa = 10.09 AIQINRR677 pKa = 11.84 KK678 pKa = 8.0 GVCIYY683 pKa = 11.03 YY684 pKa = 10.28 DD685 pKa = 3.64 AEE687 pKa = 4.46 RR688 pKa = 11.84 SDD690 pKa = 3.04 ITEE693 pKa = 3.69 YY694 pKa = 11.27 GGVVKK699 pKa = 10.42 IAKK702 pKa = 9.55 DD703 pKa = 3.21 INVTINTGEE712 pKa = 4.11 TVQRR716 pKa = 11.84 LRR718 pKa = 11.84 GYY720 pKa = 8.19 ITRR723 pKa = 11.84 EE724 pKa = 3.48 EE725 pKa = 4.01 TGFRR729 pKa = 11.84 FVGGVQAGVIPPQQLDD745 pKa = 3.33 QGVPAAGEE753 pKa = 4.04 DD754 pKa = 3.49
Molecular weight: 84.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.083
IPC2_protein 5.156
IPC_protein 5.118
Toseland 5.092
ProMoST 5.219
Dawson 5.118
Bjellqvist 5.245
Wikipedia 4.978
Rodwell 5.041
Grimsley 5.029
Solomon 5.118
Lehninger 5.08
Nozaki 5.245
DTASelect 5.372
Thurlkill 5.105
EMBOSS 5.041
Sillero 5.334
Patrickios 3.897
IPC_peptide 5.13
IPC2_peptide 5.321
IPC2.peptide.svr19 5.3
Protein with the highest isoelectric point:
>tr|A0A1L3KEZ6|A0A1L3KEZ6_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 2 OX=1923308 PE=4 SV=1
MM1 pKa = 7.01 WLYY4 pKa = 10.65 YY5 pKa = 10.62 VSTRR9 pKa = 11.84 AEE11 pKa = 4.29 AKK13 pKa = 8.77 TLPAIIRR20 pKa = 11.84 RR21 pKa = 11.84 AMSAMYY27 pKa = 10.39 SLVDD31 pKa = 4.33 GYY33 pKa = 11.35 DD34 pKa = 3.27 FSDD37 pKa = 3.72 ASGTRR42 pKa = 11.84 YY43 pKa = 9.55 LRR45 pKa = 11.84 RR46 pKa = 11.84 EE47 pKa = 3.93 FDD49 pKa = 2.83 VDD51 pKa = 3.62 RR52 pKa = 11.84 YY53 pKa = 10.2 VVTRR57 pKa = 11.84 AGKK60 pKa = 9.41 NNKK63 pKa = 8.64 PVPGEE68 pKa = 3.77 FKK70 pKa = 10.15 ISKK73 pKa = 9.9 VSAEE77 pKa = 4.05 HH78 pKa = 5.7 HH79 pKa = 4.15 THH81 pKa = 5.65 YY82 pKa = 10.42 RR83 pKa = 11.84 PEE85 pKa = 4.55 EE86 pKa = 3.74 IWEE89 pKa = 3.75 IAANFRR95 pKa = 11.84 LKK97 pKa = 9.75 MRR99 pKa = 11.84 CLSILVDD106 pKa = 3.43 KK107 pKa = 9.96 MRR109 pKa = 11.84 KK110 pKa = 8.69 IEE112 pKa = 4.12 GVTEE116 pKa = 4.27 AMVSTCMAYY125 pKa = 10.68 VLFARR130 pKa = 11.84 PQVAYY135 pKa = 9.47 IFVSSRR141 pKa = 11.84 RR142 pKa = 11.84 LWDD145 pKa = 3.54 CEE147 pKa = 4.14 NVGEE151 pKa = 4.89 LAVTLKK157 pKa = 11.0 SLATPLKK164 pKa = 10.51 SMHH167 pKa = 6.47 NGDD170 pKa = 4.61 LLDD173 pKa = 3.55 MTEE176 pKa = 3.96 MFEE179 pKa = 5.73 LNSLLNRR186 pKa = 11.84 GIGSVNWDD194 pKa = 3.31 AEE196 pKa = 4.24 RR197 pKa = 11.84 EE198 pKa = 4.24 HH199 pKa = 7.22 RR200 pKa = 11.84 QEE202 pKa = 3.98 PNVIKK207 pKa = 10.61 VSKK210 pKa = 11.07 AEE212 pKa = 3.98 VYY214 pKa = 9.85 KK215 pKa = 10.06 QAKK218 pKa = 9.08 IVFSEE223 pKa = 4.99 GINHH227 pKa = 6.82 GYY229 pKa = 10.28 KK230 pKa = 10.28 YY231 pKa = 11.04 NKK233 pKa = 10.02 LSLDD237 pKa = 3.6 RR238 pKa = 11.84 YY239 pKa = 10.54 LEE241 pKa = 4.28 SRR243 pKa = 11.84 WEE245 pKa = 4.04 WVPSGSVHH253 pKa = 5.37 SQYY256 pKa = 10.95 PEE258 pKa = 3.96 DD259 pKa = 3.4 SPYY262 pKa = 10.09 IKK264 pKa = 10.14 KK265 pKa = 10.23 EE266 pKa = 3.62 YY267 pKa = 9.1 RR268 pKa = 11.84 HH269 pKa = 4.99 RR270 pKa = 11.84 TKK272 pKa = 10.61 FVTLNKK278 pKa = 9.41 MPRR281 pKa = 11.84 SHH283 pKa = 6.83 IAGMFKK289 pKa = 10.49 RR290 pKa = 11.84 KK291 pKa = 9.72 PEE293 pKa = 3.78 IQAWASVKK301 pKa = 10.47 YY302 pKa = 9.31 EE303 pKa = 3.98 WAKK306 pKa = 9.76 MRR308 pKa = 11.84 AIYY311 pKa = 10.08 GVDD314 pKa = 3.36 LTSSVITNFAMYY326 pKa = 10.0 RR327 pKa = 11.84 CEE329 pKa = 4.02 EE330 pKa = 4.23 VFKK333 pKa = 11.11 HH334 pKa = 6.01 RR335 pKa = 11.84 FPLGEE340 pKa = 3.66 EE341 pKa = 3.65 AAADD345 pKa = 3.92 RR346 pKa = 11.84 VHH348 pKa = 6.67 KK349 pKa = 10.15 RR350 pKa = 11.84 LKK352 pKa = 10.6 IMLKK356 pKa = 10.37 DD357 pKa = 3.93 SEE359 pKa = 4.64 SLCYY363 pKa = 10.81 DD364 pKa = 3.49 FDD366 pKa = 4.83 DD367 pKa = 5.96 FNAQHH372 pKa = 5.78 STEE375 pKa = 4.01 AMQAVLEE382 pKa = 4.68 AYY384 pKa = 10.38 CDD386 pKa = 3.37 TFYY389 pKa = 11.63 YY390 pKa = 11.09 DD391 pKa = 2.99 MTAEE395 pKa = 3.88 QRR397 pKa = 11.84 VAMKK401 pKa = 8.76 WVCDD405 pKa = 3.8 SVKK408 pKa = 9.81 NTTIHH413 pKa = 5.94 NNEE416 pKa = 4.39 DD417 pKa = 3.61 GRR419 pKa = 11.84 CEE421 pKa = 3.89 KK422 pKa = 11.13 YY423 pKa = 9.14 NTNGTLLSGWRR434 pKa = 11.84 LTTFMNTALNYY445 pKa = 9.75 IYY447 pKa = 10.44 FKK449 pKa = 10.47 IAGIFEE455 pKa = 4.32 IDD457 pKa = 3.55 GVHH460 pKa = 7.33 DD461 pKa = 4.15 SVHH464 pKa = 6.54 NGDD467 pKa = 4.57 DD468 pKa = 3.46 VLVAINNIKK477 pKa = 8.15 TANVVHH483 pKa = 6.23 EE484 pKa = 4.46 RR485 pKa = 11.84 MRR487 pKa = 11.84 LINARR492 pKa = 11.84 AQPAKK497 pKa = 10.67 CNVFSVGEE505 pKa = 3.91 FLRR508 pKa = 11.84 VEE510 pKa = 4.1 HH511 pKa = 7.09 KK512 pKa = 10.57 LDD514 pKa = 3.44 KK515 pKa = 11.01 EE516 pKa = 4.24 KK517 pKa = 11.28 GLGAQYY523 pKa = 9.06 LTRR526 pKa = 11.84 GVATLVHH533 pKa = 6.23 SRR535 pKa = 11.84 VEE537 pKa = 4.27 SQEE540 pKa = 3.62 PTRR543 pKa = 11.84 LLEE546 pKa = 4.19 ALKK549 pKa = 10.64 AATTRR554 pKa = 11.84 CEE556 pKa = 3.81 EE557 pKa = 3.81 VAQRR561 pKa = 11.84 SRR563 pKa = 11.84 CGVDD567 pKa = 3.06 TAAAFLDD574 pKa = 3.93 KK575 pKa = 10.87 AVEE578 pKa = 4.0 RR579 pKa = 11.84 MSNIFGVEE587 pKa = 3.61 RR588 pKa = 11.84 EE589 pKa = 3.92 ICNRR593 pKa = 11.84 AVQAHH598 pKa = 6.2 VIVGGPIASRR608 pKa = 11.84 EE609 pKa = 3.93 GKK611 pKa = 9.6 IDD613 pKa = 3.53 FEE615 pKa = 4.47 YY616 pKa = 11.19 FEE618 pKa = 5.78 DD619 pKa = 3.42 IQYY622 pKa = 11.04 EE623 pKa = 4.19 DD624 pKa = 4.14 EE625 pKa = 4.09 EE626 pKa = 4.99 RR627 pKa = 11.84 MSEE630 pKa = 4.0 EE631 pKa = 3.81 MRR633 pKa = 11.84 EE634 pKa = 3.75 KK635 pKa = 10.49 RR636 pKa = 11.84 ATVRR640 pKa = 11.84 EE641 pKa = 4.63 LNPGIYY647 pKa = 9.66 DD648 pKa = 3.76 YY649 pKa = 11.66 CSVLYY654 pKa = 9.96 SQYY657 pKa = 11.47 GEE659 pKa = 3.97 FVPRR663 pKa = 11.84 GKK665 pKa = 9.96 ILLSILGATRR675 pKa = 11.84 RR676 pKa = 11.84 QLAITRR682 pKa = 11.84 RR683 pKa = 11.84 TWLRR687 pKa = 11.84 EE688 pKa = 3.34 VDD690 pKa = 3.46 VRR692 pKa = 11.84 AEE694 pKa = 3.74 GRR696 pKa = 11.84 YY697 pKa = 9.55 KK698 pKa = 10.63 FGRR701 pKa = 11.84 ALFRR705 pKa = 11.84 MYY707 pKa = 10.42 RR708 pKa = 11.84 GIIDD712 pKa = 3.37 VPFIEE717 pKa = 4.51 KK718 pKa = 10.49 ARR720 pKa = 11.84 FVGISPISMISDD732 pKa = 3.7 SSARR736 pKa = 11.84 LIKK739 pKa = 10.31 QLISHH744 pKa = 6.05 VVDD747 pKa = 2.96 VDD749 pKa = 3.41 YY750 pKa = 10.65 TLRR753 pKa = 11.84 VLLL756 pKa = 4.58
Molecular weight: 87.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.584
IPC2_protein 7.922
IPC_protein 7.746
Toseland 7.541
ProMoST 8.317
Dawson 8.507
Bjellqvist 8.756
Wikipedia 8.478
Rodwell 8.551
Grimsley 7.6
Solomon 8.565
Lehninger 8.565
Nozaki 8.843
DTASelect 8.536
Thurlkill 8.58
EMBOSS 8.682
Sillero 8.858
Patrickios 4.393
IPC_peptide 8.565
IPC2_peptide 7.614
IPC2.peptide.svr19 7.857
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1510
754
756
755.0
85.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.616 ± 0.311
1.457 ± 0.18
4.967 ± 0.041
7.881 ± 0.129
3.907 ± 0.042
6.225 ± 0.822
2.318 ± 0.225
5.298 ± 0.358
5.563 ± 0.267
8.013 ± 0.415
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.444 ± 0.094
4.04 ± 0.049
3.444 ± 0.547
3.179 ± 0.638
6.424 ± 1.4
6.623 ± 0.097
5.695 ± 0.64
7.616 ± 0.401
1.788 ± 0.228
4.503 ± 0.177
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here