Hubei toti-like virus 2

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KEZ6|A0A1L3KEZ6_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 2 OX=1923308 PE=4 SV=1
MM1 pKa = 7.97DD2 pKa = 4.68GFVKK6 pKa = 10.32RR7 pKa = 11.84FYY9 pKa = 10.07PEE11 pKa = 3.87HH12 pKa = 6.33EE13 pKa = 4.15APGIEE18 pKa = 4.46FKK20 pKa = 11.22SEE22 pKa = 3.45GDD24 pKa = 3.73LKK26 pKa = 11.07FGTYY30 pKa = 9.69YY31 pKa = 10.99GGVLRR36 pKa = 11.84VFDD39 pKa = 3.81GHH41 pKa = 5.36VQSSRR46 pKa = 11.84NHH48 pKa = 5.59YY49 pKa = 10.06AVQEE53 pKa = 3.92MTAAGKK59 pKa = 10.23LKK61 pKa = 10.26AVLMGTSSSLDD72 pKa = 3.43GLTKK76 pKa = 10.36QYY78 pKa = 8.15LTPEE82 pKa = 3.94GMVNISTVAEE92 pKa = 4.04ALKK95 pKa = 10.62QSAGLQTQVWSTHH108 pKa = 4.22VNRR111 pKa = 11.84MQHH114 pKa = 5.94WGWADD119 pKa = 3.19NHH121 pKa = 6.45VSLLVNLLRR130 pKa = 11.84FSILLRR136 pKa = 11.84TQEE139 pKa = 4.39SNGGNLDD146 pKa = 3.73CSLGVYY152 pKa = 9.43MDD154 pKa = 3.03GHH156 pKa = 7.06VNVDD160 pKa = 3.09MDD162 pKa = 3.77QWWSSEE168 pKa = 3.91YY169 pKa = 9.65PEE171 pKa = 4.79KK172 pKa = 11.14YY173 pKa = 10.72KK174 pKa = 10.28MDD176 pKa = 3.48TWPTGTGEE184 pKa = 4.11EE185 pKa = 4.48NYY187 pKa = 10.15PEE189 pKa = 4.3YY190 pKa = 11.37AHH192 pKa = 6.45LTSTTPDD199 pKa = 3.36TDD201 pKa = 3.8SNAIDD206 pKa = 4.13LRR208 pKa = 11.84GLTAVEE214 pKa = 3.81ANIILMMTGAWSRR227 pKa = 11.84KK228 pKa = 6.83SRR230 pKa = 11.84CRR232 pKa = 11.84LDD234 pKa = 3.65FSTPRR239 pKa = 11.84LTEE242 pKa = 3.63LLYY245 pKa = 10.44YY246 pKa = 10.38RR247 pKa = 11.84HH248 pKa = 6.57GSTMTGLEE256 pKa = 3.92SWLKK260 pKa = 9.51EE261 pKa = 3.97EE262 pKa = 5.4QGAEE266 pKa = 4.1MPKK269 pKa = 9.34MPNWKK274 pKa = 9.38QVWSALRR281 pKa = 11.84AYY283 pKa = 10.1VGQNRR288 pKa = 11.84LYY290 pKa = 10.71DD291 pKa = 3.63QFSTALYY298 pKa = 9.21IVSFLAYY305 pKa = 9.89QFVPATAEE313 pKa = 3.86ACVWLSFDD321 pKa = 3.47WQIVMPVFHH330 pKa = 7.51SIRR333 pKa = 11.84GRR335 pKa = 11.84YY336 pKa = 8.45EE337 pKa = 3.42ILNDD341 pKa = 3.64GEE343 pKa = 4.29AALVSHH349 pKa = 7.01RR350 pKa = 11.84AINEE354 pKa = 3.58WGFINGKK361 pKa = 8.27LEE363 pKa = 4.67KK364 pKa = 10.15INLMCMLVAQCFQTGCAVRR383 pKa = 11.84AMRR386 pKa = 11.84KK387 pKa = 9.51GLEE390 pKa = 4.11TDD392 pKa = 3.03PHH394 pKa = 7.15DD395 pKa = 4.62LYY397 pKa = 11.02STEE400 pKa = 4.14TEE402 pKa = 4.77FYY404 pKa = 9.79SASSFMSAAVSEE416 pKa = 4.54ATRR419 pKa = 11.84SEE421 pKa = 4.18FPMPGMAGIYY431 pKa = 10.13LRR433 pKa = 11.84TDD435 pKa = 2.77VRR437 pKa = 11.84FDD439 pKa = 3.93DD440 pKa = 4.39PGEE443 pKa = 3.97FQIMTLNTSEE453 pKa = 4.77RR454 pKa = 11.84GLEE457 pKa = 4.42GYY459 pKa = 8.36DD460 pKa = 3.36TEE462 pKa = 4.76VIEE465 pKa = 5.41SKK467 pKa = 9.81TKK469 pKa = 10.12EE470 pKa = 3.61IKK472 pKa = 10.0KK473 pKa = 8.42VHH475 pKa = 6.5KK476 pKa = 9.93EE477 pKa = 3.74LSDD480 pKa = 3.84LEE482 pKa = 4.65GKK484 pKa = 9.67EE485 pKa = 4.24KK486 pKa = 10.7YY487 pKa = 10.41AEE489 pKa = 4.17ALAEE493 pKa = 3.88ISMLKK498 pKa = 10.17QIAEE502 pKa = 4.35IKK504 pKa = 10.38LQMKK508 pKa = 10.4SSTLSYY514 pKa = 10.17QEE516 pKa = 4.08KK517 pKa = 9.94LAVEE521 pKa = 4.06NKK523 pKa = 9.64YY524 pKa = 10.58VVEE527 pKa = 4.39TEE529 pKa = 4.02VEE531 pKa = 4.31TTVNLIAVKK540 pKa = 9.88CVWVPMAGSPVLALPLNPFPYY561 pKa = 10.35NSPLTLKK568 pKa = 9.85GTIEE572 pKa = 3.96PAMGSLSRR580 pKa = 11.84KK581 pKa = 7.75GFKK584 pKa = 10.45VSADD588 pKa = 3.86KK589 pKa = 11.08AWLIANFTRR598 pKa = 11.84LCGYY602 pKa = 10.35DD603 pKa = 3.47VLFKK607 pKa = 10.6QHH609 pKa = 6.56GEE611 pKa = 3.82QAGPSDD617 pKa = 3.94YY618 pKa = 10.69FAPNNVNMVWPILATDD634 pKa = 4.54DD635 pKa = 5.16DD636 pKa = 4.33QDD638 pKa = 3.73EE639 pKa = 5.43PITITGMEE647 pKa = 3.89EE648 pKa = 3.68RR649 pKa = 11.84DD650 pKa = 3.58RR651 pKa = 11.84LFVSLPPIHH660 pKa = 6.78NKK662 pKa = 9.41FFSNQKK668 pKa = 10.13LEE670 pKa = 3.83YY671 pKa = 10.09AIQINRR677 pKa = 11.84KK678 pKa = 8.0GVCIYY683 pKa = 11.03YY684 pKa = 10.28DD685 pKa = 3.64AEE687 pKa = 4.46RR688 pKa = 11.84SDD690 pKa = 3.04ITEE693 pKa = 3.69YY694 pKa = 11.27GGVVKK699 pKa = 10.42IAKK702 pKa = 9.55DD703 pKa = 3.21INVTINTGEE712 pKa = 4.11TVQRR716 pKa = 11.84LRR718 pKa = 11.84GYY720 pKa = 8.19ITRR723 pKa = 11.84EE724 pKa = 3.48EE725 pKa = 4.01TGFRR729 pKa = 11.84FVGGVQAGVIPPQQLDD745 pKa = 3.33QGVPAAGEE753 pKa = 4.04DD754 pKa = 3.49

Molecular weight:
84.74 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KEZ6|A0A1L3KEZ6_9VIRU RNA-directed RNA polymerase OS=Hubei toti-like virus 2 OX=1923308 PE=4 SV=1
MM1 pKa = 7.01WLYY4 pKa = 10.65YY5 pKa = 10.62VSTRR9 pKa = 11.84AEE11 pKa = 4.29AKK13 pKa = 8.77TLPAIIRR20 pKa = 11.84RR21 pKa = 11.84AMSAMYY27 pKa = 10.39SLVDD31 pKa = 4.33GYY33 pKa = 11.35DD34 pKa = 3.27FSDD37 pKa = 3.72ASGTRR42 pKa = 11.84YY43 pKa = 9.55LRR45 pKa = 11.84RR46 pKa = 11.84EE47 pKa = 3.93FDD49 pKa = 2.83VDD51 pKa = 3.62RR52 pKa = 11.84YY53 pKa = 10.2VVTRR57 pKa = 11.84AGKK60 pKa = 9.41NNKK63 pKa = 8.64PVPGEE68 pKa = 3.77FKK70 pKa = 10.15ISKK73 pKa = 9.9VSAEE77 pKa = 4.05HH78 pKa = 5.7HH79 pKa = 4.15THH81 pKa = 5.65YY82 pKa = 10.42RR83 pKa = 11.84PEE85 pKa = 4.55EE86 pKa = 3.74IWEE89 pKa = 3.75IAANFRR95 pKa = 11.84LKK97 pKa = 9.75MRR99 pKa = 11.84CLSILVDD106 pKa = 3.43KK107 pKa = 9.96MRR109 pKa = 11.84KK110 pKa = 8.69IEE112 pKa = 4.12GVTEE116 pKa = 4.27AMVSTCMAYY125 pKa = 10.68VLFARR130 pKa = 11.84PQVAYY135 pKa = 9.47IFVSSRR141 pKa = 11.84RR142 pKa = 11.84LWDD145 pKa = 3.54CEE147 pKa = 4.14NVGEE151 pKa = 4.89LAVTLKK157 pKa = 11.0SLATPLKK164 pKa = 10.51SMHH167 pKa = 6.47NGDD170 pKa = 4.61LLDD173 pKa = 3.55MTEE176 pKa = 3.96MFEE179 pKa = 5.73LNSLLNRR186 pKa = 11.84GIGSVNWDD194 pKa = 3.31AEE196 pKa = 4.24RR197 pKa = 11.84EE198 pKa = 4.24HH199 pKa = 7.22RR200 pKa = 11.84QEE202 pKa = 3.98PNVIKK207 pKa = 10.61VSKK210 pKa = 11.07AEE212 pKa = 3.98VYY214 pKa = 9.85KK215 pKa = 10.06QAKK218 pKa = 9.08IVFSEE223 pKa = 4.99GINHH227 pKa = 6.82GYY229 pKa = 10.28KK230 pKa = 10.28YY231 pKa = 11.04NKK233 pKa = 10.02LSLDD237 pKa = 3.6RR238 pKa = 11.84YY239 pKa = 10.54LEE241 pKa = 4.28SRR243 pKa = 11.84WEE245 pKa = 4.04WVPSGSVHH253 pKa = 5.37SQYY256 pKa = 10.95PEE258 pKa = 3.96DD259 pKa = 3.4SPYY262 pKa = 10.09IKK264 pKa = 10.14KK265 pKa = 10.23EE266 pKa = 3.62YY267 pKa = 9.1RR268 pKa = 11.84HH269 pKa = 4.99RR270 pKa = 11.84TKK272 pKa = 10.61FVTLNKK278 pKa = 9.41MPRR281 pKa = 11.84SHH283 pKa = 6.83IAGMFKK289 pKa = 10.49RR290 pKa = 11.84KK291 pKa = 9.72PEE293 pKa = 3.78IQAWASVKK301 pKa = 10.47YY302 pKa = 9.31EE303 pKa = 3.98WAKK306 pKa = 9.76MRR308 pKa = 11.84AIYY311 pKa = 10.08GVDD314 pKa = 3.36LTSSVITNFAMYY326 pKa = 10.0RR327 pKa = 11.84CEE329 pKa = 4.02EE330 pKa = 4.23VFKK333 pKa = 11.11HH334 pKa = 6.01RR335 pKa = 11.84FPLGEE340 pKa = 3.66EE341 pKa = 3.65AAADD345 pKa = 3.92RR346 pKa = 11.84VHH348 pKa = 6.67KK349 pKa = 10.15RR350 pKa = 11.84LKK352 pKa = 10.6IMLKK356 pKa = 10.37DD357 pKa = 3.93SEE359 pKa = 4.64SLCYY363 pKa = 10.81DD364 pKa = 3.49FDD366 pKa = 4.83DD367 pKa = 5.96FNAQHH372 pKa = 5.78STEE375 pKa = 4.01AMQAVLEE382 pKa = 4.68AYY384 pKa = 10.38CDD386 pKa = 3.37TFYY389 pKa = 11.63YY390 pKa = 11.09DD391 pKa = 2.99MTAEE395 pKa = 3.88QRR397 pKa = 11.84VAMKK401 pKa = 8.76WVCDD405 pKa = 3.8SVKK408 pKa = 9.81NTTIHH413 pKa = 5.94NNEE416 pKa = 4.39DD417 pKa = 3.61GRR419 pKa = 11.84CEE421 pKa = 3.89KK422 pKa = 11.13YY423 pKa = 9.14NTNGTLLSGWRR434 pKa = 11.84LTTFMNTALNYY445 pKa = 9.75IYY447 pKa = 10.44FKK449 pKa = 10.47IAGIFEE455 pKa = 4.32IDD457 pKa = 3.55GVHH460 pKa = 7.33DD461 pKa = 4.15SVHH464 pKa = 6.54NGDD467 pKa = 4.57DD468 pKa = 3.46VLVAINNIKK477 pKa = 8.15TANVVHH483 pKa = 6.23EE484 pKa = 4.46RR485 pKa = 11.84MRR487 pKa = 11.84LINARR492 pKa = 11.84AQPAKK497 pKa = 10.67CNVFSVGEE505 pKa = 3.91FLRR508 pKa = 11.84VEE510 pKa = 4.1HH511 pKa = 7.09KK512 pKa = 10.57LDD514 pKa = 3.44KK515 pKa = 11.01EE516 pKa = 4.24KK517 pKa = 11.28GLGAQYY523 pKa = 9.06LTRR526 pKa = 11.84GVATLVHH533 pKa = 6.23SRR535 pKa = 11.84VEE537 pKa = 4.27SQEE540 pKa = 3.62PTRR543 pKa = 11.84LLEE546 pKa = 4.19ALKK549 pKa = 10.64AATTRR554 pKa = 11.84CEE556 pKa = 3.81EE557 pKa = 3.81VAQRR561 pKa = 11.84SRR563 pKa = 11.84CGVDD567 pKa = 3.06TAAAFLDD574 pKa = 3.93KK575 pKa = 10.87AVEE578 pKa = 4.0RR579 pKa = 11.84MSNIFGVEE587 pKa = 3.61RR588 pKa = 11.84EE589 pKa = 3.92ICNRR593 pKa = 11.84AVQAHH598 pKa = 6.2VIVGGPIASRR608 pKa = 11.84EE609 pKa = 3.93GKK611 pKa = 9.6IDD613 pKa = 3.53FEE615 pKa = 4.47YY616 pKa = 11.19FEE618 pKa = 5.78DD619 pKa = 3.42IQYY622 pKa = 11.04EE623 pKa = 4.19DD624 pKa = 4.14EE625 pKa = 4.09EE626 pKa = 4.99RR627 pKa = 11.84MSEE630 pKa = 4.0EE631 pKa = 3.81MRR633 pKa = 11.84EE634 pKa = 3.75KK635 pKa = 10.49RR636 pKa = 11.84ATVRR640 pKa = 11.84EE641 pKa = 4.63LNPGIYY647 pKa = 9.66DD648 pKa = 3.76YY649 pKa = 11.66CSVLYY654 pKa = 9.96SQYY657 pKa = 11.47GEE659 pKa = 3.97FVPRR663 pKa = 11.84GKK665 pKa = 9.96ILLSILGATRR675 pKa = 11.84RR676 pKa = 11.84QLAITRR682 pKa = 11.84RR683 pKa = 11.84TWLRR687 pKa = 11.84EE688 pKa = 3.34VDD690 pKa = 3.46VRR692 pKa = 11.84AEE694 pKa = 3.74GRR696 pKa = 11.84YY697 pKa = 9.55KK698 pKa = 10.63FGRR701 pKa = 11.84ALFRR705 pKa = 11.84MYY707 pKa = 10.42RR708 pKa = 11.84GIIDD712 pKa = 3.37VPFIEE717 pKa = 4.51KK718 pKa = 10.49ARR720 pKa = 11.84FVGISPISMISDD732 pKa = 3.7SSARR736 pKa = 11.84LIKK739 pKa = 10.31QLISHH744 pKa = 6.05VVDD747 pKa = 2.96VDD749 pKa = 3.41YY750 pKa = 10.65TLRR753 pKa = 11.84VLLL756 pKa = 4.58

Molecular weight:
87.15 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1510

754

756

755.0

85.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.616 ± 0.311

1.457 ± 0.18

4.967 ± 0.041

7.881 ± 0.129

3.907 ± 0.042

6.225 ± 0.822

2.318 ± 0.225

5.298 ± 0.358

5.563 ± 0.267

8.013 ± 0.415

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.444 ± 0.094

4.04 ± 0.049

3.444 ± 0.547

3.179 ± 0.638

6.424 ± 1.4

6.623 ± 0.097

5.695 ± 0.64

7.616 ± 0.401

1.788 ± 0.228

4.503 ± 0.177

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski