Parasponia andersonii (Sponia andersonii)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Rosales; Cannabaceae; Parasponia

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37181 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P5B153|A0A2P5B153_PARAD Acid phosphatase/vanadium-dependent haloperoxidase-related OS=Parasponia andersonii OX=3476 GN=PanWU01x14_280960 PE=4 SV=1
MM1 pKa = 7.73EE2 pKa = 5.27SPSAGDD8 pKa = 3.59HH9 pKa = 6.95DD10 pKa = 4.42LAIYY14 pKa = 9.97MDD16 pKa = 4.5DD17 pKa = 3.74TQHH20 pKa = 7.17PDD22 pKa = 2.81SSNDD26 pKa = 3.03AVLDD30 pKa = 3.83HH31 pKa = 7.25LIPPPPPPPPSDD43 pKa = 3.84RR44 pKa = 11.84DD45 pKa = 3.6VCLLFQLILGFVCAFGILISLALLIFLIIFGSS77 pKa = 3.48

Molecular weight: 8.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P5DUB9|A0A2P5DUB9_PARAD Uncharacterized protein (Fragment) OS=Parasponia andersonii OX=3476 GN=PanWU01x14_031790 PE=4 SV=1
MM1 pKa = 7.41LHH3 pKa = 5.95QASCLVVVRR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84GRR16 pKa = 11.84RR17 pKa = 11.84LVLVNRR23 pKa = 11.84RR24 pKa = 11.84RR25 pKa = 11.84WWRR28 pKa = 11.84RR29 pKa = 11.84LVVIWLLRR37 pKa = 11.84GRR39 pKa = 11.84GHH41 pKa = 7.14RR42 pKa = 11.84GRR44 pKa = 11.84VVRR47 pKa = 11.84LLWWWWWWRR56 pKa = 11.84LIMVRR61 pKa = 11.84LLWRR65 pKa = 11.84RR66 pKa = 11.84WNWRR70 pKa = 11.84RR71 pKa = 11.84VVRR74 pKa = 11.84LLWWWWRR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84LVVVWLLRR91 pKa = 11.84WRR93 pKa = 11.84GYY95 pKa = 9.26RR96 pKa = 11.84GRR98 pKa = 11.84VIGLFGWWWWWRR110 pKa = 11.84LVMVRR115 pKa = 11.84LLRR118 pKa = 11.84GRR120 pKa = 11.84GHH122 pKa = 7.2WGRR125 pKa = 11.84VVGLLWWRR133 pKa = 11.84WWWW136 pKa = 3.29

Molecular weight: 18.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37178

3

37181

11013480

24

5535

296.2

33.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.586 ± 0.013

1.837 ± 0.006

5.19 ± 0.01

6.408 ± 0.018

4.309 ± 0.011

6.538 ± 0.015

2.409 ± 0.008

5.283 ± 0.009

5.976 ± 0.015

9.92 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.006

4.423 ± 0.009

4.923 ± 0.013

3.563 ± 0.009

5.542 ± 0.011

9.141 ± 0.016

4.945 ± 0.009

6.604 ± 0.01

1.315 ± 0.005

2.748 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski