Apis mellifera associated microvirus 23

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S8UTX4|A0A3S8UTX4_9VIRU Internal scaffolding protein OS=Apis mellifera associated microvirus 23 OX=2494751 PE=4 SV=1
MM1 pKa = 7.4LGSRR5 pKa = 11.84MSQHH9 pKa = 6.3KK10 pKa = 8.96FANVPAVNTARR21 pKa = 11.84SSFDD25 pKa = 2.99RR26 pKa = 11.84SFQIKK31 pKa = 8.78DD32 pKa = 3.42TFDD35 pKa = 3.54FDD37 pKa = 3.92YY38 pKa = 10.84LIPIFVDD45 pKa = 4.73EE46 pKa = 4.88ILPGDD51 pKa = 3.75TCNVRR56 pKa = 11.84MSCFGRR62 pKa = 11.84LATQLVPIMDD72 pKa = 4.07NMYY75 pKa = 9.86IDD77 pKa = 3.75YY78 pKa = 10.82FFFFVPNRR86 pKa = 11.84LVWDD90 pKa = 3.1NWEE93 pKa = 4.33RR94 pKa = 11.84FNGAQDD100 pKa = 4.11DD101 pKa = 4.68PGDD104 pKa = 4.02STDD107 pKa = 3.85FTIPQIEE114 pKa = 4.68FATRR118 pKa = 11.84PQVGDD123 pKa = 3.29IYY125 pKa = 11.43DD126 pKa = 3.81HH127 pKa = 6.93FGLPTGLTNTWYY139 pKa = 10.94CNALPFRR146 pKa = 11.84CYY148 pKa = 11.02SLIWNEE154 pKa = 3.67WFRR157 pKa = 11.84DD158 pKa = 3.62QNLQSSVPVPKK169 pKa = 10.72DD170 pKa = 3.24NGPDD174 pKa = 3.25VYY176 pKa = 11.6ADD178 pKa = 3.56FTLLKK183 pKa = 9.88RR184 pKa = 11.84GKK186 pKa = 9.0RR187 pKa = 11.84HH188 pKa = 7.53DD189 pKa = 4.2YY190 pKa = 8.21FTSCLPWPQKK200 pKa = 11.05GPDD203 pKa = 3.33VTLPLASQAPVKK215 pKa = 10.81SLGVVGNDD223 pKa = 3.2YY224 pKa = 11.44VGVLDD229 pKa = 3.74STNTPRR235 pKa = 11.84RR236 pKa = 11.84LQDD239 pKa = 2.98NGTAIIIAAGSPTASADD256 pKa = 3.4LFADD260 pKa = 4.45LNQATAATINQLRR273 pKa = 11.84EE274 pKa = 3.99AFLMQSLFEE283 pKa = 5.07LDD285 pKa = 3.28ARR287 pKa = 11.84GGTRR291 pKa = 11.84YY292 pKa = 9.98VEE294 pKa = 3.69ILQAHH299 pKa = 6.38FNVTSPDD306 pKa = 3.15FRR308 pKa = 11.84LQRR311 pKa = 11.84PEE313 pKa = 3.69FLGGGSSRR321 pKa = 11.84INSHH325 pKa = 6.57IVPQTSPTSGSDD337 pKa = 3.4PQGQLAAFATTSADD351 pKa = 3.62GIGFTKK357 pKa = 10.61SFVEE361 pKa = 3.76HH362 pKa = 6.81GYY364 pKa = 11.06VIGLACARR372 pKa = 11.84ADD374 pKa = 3.33VTYY377 pKa = 10.54QQGLNRR383 pKa = 11.84MWSRR387 pKa = 11.84STRR390 pKa = 11.84YY391 pKa = 10.5DD392 pKa = 3.47FFWPKK397 pKa = 10.2LQEE400 pKa = 4.22LGEE403 pKa = 4.13QEE405 pKa = 4.18VLNRR409 pKa = 11.84EE410 pKa = 4.12IYY412 pKa = 10.53LQGTADD418 pKa = 3.83DD419 pKa = 4.28EE420 pKa = 5.03AVFGYY425 pKa = 7.37QEE427 pKa = 4.18RR428 pKa = 11.84YY429 pKa = 9.58AEE431 pKa = 4.06YY432 pKa = 9.72RR433 pKa = 11.84YY434 pKa = 10.26KK435 pKa = 10.54PSEE438 pKa = 3.37IRR440 pKa = 11.84GQFRR444 pKa = 11.84STYY447 pKa = 9.06STSLDD452 pKa = 3.35FWHH455 pKa = 6.56MAEE458 pKa = 4.67EE459 pKa = 4.63FGSLPALNDD468 pKa = 3.25TFIQQNTPVDD478 pKa = 3.63RR479 pKa = 11.84AIAIPTEE486 pKa = 3.66PHH488 pKa = 6.48LLFDD492 pKa = 4.82AYY494 pKa = 10.7FKK496 pKa = 9.05YY497 pKa = 8.69THH499 pKa = 6.04VRR501 pKa = 11.84PMMTYY506 pKa = 8.51STPASLGRR514 pKa = 11.84FF515 pKa = 3.67

Molecular weight: 58.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTW3|A0A3S8UTW3_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 23 OX=2494751 PE=3 SV=1
MM1 pKa = 7.41EE2 pKa = 5.03GVRR5 pKa = 11.84EE6 pKa = 4.78AIACPWKK13 pKa = 10.5VKK15 pKa = 10.36KK16 pKa = 10.41IYY18 pKa = 9.63LQKK21 pKa = 10.96NLYY24 pKa = 10.06KK25 pKa = 10.53LACNQQIAGNEE36 pKa = 3.83NSASMRR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 10.11AVMQRR49 pKa = 11.84AQYY52 pKa = 9.57EE53 pKa = 4.16NWKK56 pKa = 10.08KK57 pKa = 9.89RR58 pKa = 11.84VRR60 pKa = 11.84RR61 pKa = 11.84EE62 pKa = 3.76FKK64 pKa = 10.63KK65 pKa = 10.88LAGEE69 pKa = 4.28TKK71 pKa = 10.23KK72 pKa = 11.02VEE74 pKa = 4.1GLGWSQYY81 pKa = 9.53LDD83 pKa = 3.35KK84 pKa = 10.61RR85 pKa = 11.84VKK87 pKa = 10.42EE88 pKa = 3.64IGFRR92 pKa = 11.84SINHH96 pKa = 6.48LCAEE100 pKa = 4.81LSQQ103 pKa = 3.75

Molecular weight: 12.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1398

90

515

233.0

26.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.369 ± 1.394

1.359 ± 0.486

6.295 ± 0.801

5.508 ± 0.739

5.079 ± 1.05

6.009 ± 0.623

1.86 ± 0.41

4.649 ± 0.43

7.01 ± 2.162

7.296 ± 0.458

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.427

4.864 ± 0.989

5.007 ± 0.784

5.222 ± 0.298

6.652 ± 0.563

6.652 ± 0.577

5.365 ± 0.934

4.936 ± 0.519

1.502 ± 0.457

4.077 ± 0.722
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski