Amino acid dipepetide frequency for Bacillus virus SRT01hs

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.814AlaAla: 0.814 ± 0.262
0.488AlaCys: 0.488 ± 0.268
2.441AlaAsp: 2.441 ± 0.589
2.929AlaGlu: 2.929 ± 0.742
2.603AlaPhe: 2.603 ± 0.666
3.742AlaGly: 3.742 ± 1.0
1.79AlaHis: 1.79 ± 0.655
3.417AlaIle: 3.417 ± 0.573
4.719AlaLys: 4.719 ± 1.137
3.254AlaLeu: 3.254 ± 0.791
1.302AlaMet: 1.302 ± 0.505
4.393AlaAsn: 4.393 ± 1.205
0.651AlaPro: 0.651 ± 0.385
2.441AlaGln: 2.441 ± 0.465
3.091AlaArg: 3.091 ± 0.679
3.742AlaSer: 3.742 ± 1.02
4.719AlaThr: 4.719 ± 0.949
4.881AlaVal: 4.881 ± 1.28
0.163AlaTrp: 0.163 ± 0.142
2.766AlaTyr: 2.766 ± 0.645
0.0AlaXaa: 0.0 ± 0.0
Cys
0.651CysAla: 0.651 ± 0.383
0.163CysCys: 0.163 ± 0.157
0.325CysAsp: 0.325 ± 0.209
0.814CysGlu: 0.814 ± 0.352
0.488CysPhe: 0.488 ± 0.282
2.115CysGly: 2.115 ± 0.941
0.0CysHis: 0.0 ± 0.0
0.488CysIle: 0.488 ± 0.307
0.325CysLys: 0.325 ± 0.255
0.325CysLeu: 0.325 ± 0.246
0.325CysMet: 0.325 ± 0.186
0.651CysAsn: 0.651 ± 0.302
0.0CysPro: 0.0 ± 0.0
0.163CysGln: 0.163 ± 0.159
0.325CysArg: 0.325 ± 0.213
0.0CysSer: 0.0 ± 0.0
0.163CysThr: 0.163 ± 0.132
1.139CysVal: 1.139 ± 0.393
0.0CysTrp: 0.0 ± 0.0
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.068AspAla: 4.068 ± 0.81
1.302AspCys: 1.302 ± 0.741
3.742AspAsp: 3.742 ± 0.699
4.393AspGlu: 4.393 ± 0.612
4.719AspPhe: 4.719 ± 1.148
4.393AspGly: 4.393 ± 0.832
0.651AspHis: 0.651 ± 0.332
3.742AspIle: 3.742 ± 0.738
3.742AspLys: 3.742 ± 0.741
5.532AspLeu: 5.532 ± 0.695
1.139AspMet: 1.139 ± 0.424
3.58AspAsn: 3.58 ± 0.557
1.627AspPro: 1.627 ± 0.574
0.976AspGln: 0.976 ± 0.421
1.79AspArg: 1.79 ± 0.509
2.929AspSer: 2.929 ± 0.663
3.58AspThr: 3.58 ± 0.653
4.881AspVal: 4.881 ± 0.687
0.488AspTrp: 0.488 ± 0.227
3.58AspTyr: 3.58 ± 0.702
0.0AspXaa: 0.0 ± 0.0
Glu
5.207GluAla: 5.207 ± 0.829
0.651GluCys: 0.651 ± 0.318
4.556GluAsp: 4.556 ± 0.931
8.298GluGlu: 8.298 ± 1.437
4.068GluPhe: 4.068 ± 0.932
4.719GluGly: 4.719 ± 0.781
1.139GluHis: 1.139 ± 0.321
4.719GluIle: 4.719 ± 0.705
4.881GluLys: 4.881 ± 1.028
6.346GluLeu: 6.346 ± 1.092
2.441GluMet: 2.441 ± 0.558
5.044GluAsn: 5.044 ± 0.959
1.79GluPro: 1.79 ± 0.544
2.603GluGln: 2.603 ± 0.663
2.603GluArg: 2.603 ± 0.622
4.881GluSer: 4.881 ± 0.891
4.881GluThr: 4.881 ± 0.854
4.881GluVal: 4.881 ± 0.802
1.464GluTrp: 1.464 ± 0.558
3.417GluTyr: 3.417 ± 0.865
0.0GluXaa: 0.0 ± 0.0
Phe
3.091PheAla: 3.091 ± 0.626
0.325PheCys: 0.325 ± 0.223
3.742PheAsp: 3.742 ± 0.706
3.254PheGlu: 3.254 ± 0.766
1.627PhePhe: 1.627 ± 0.683
2.278PheGly: 2.278 ± 0.718
1.139PheHis: 1.139 ± 0.276
3.254PheIle: 3.254 ± 0.639
3.905PheLys: 3.905 ± 0.759
3.254PheLeu: 3.254 ± 0.693
1.952PheMet: 1.952 ± 0.592
3.905PheAsn: 3.905 ± 0.84
0.814PhePro: 0.814 ± 0.279
1.464PheGln: 1.464 ± 0.399
2.278PheArg: 2.278 ± 0.587
2.115PheSer: 2.115 ± 0.488
4.068PheThr: 4.068 ± 0.727
2.766PheVal: 2.766 ± 0.541
0.325PheTrp: 0.325 ± 0.205
2.115PheTyr: 2.115 ± 0.523
0.0PheXaa: 0.0 ± 0.0
Gly
2.766GlyAla: 2.766 ± 0.85
0.651GlyCys: 0.651 ± 0.271
3.58GlyAsp: 3.58 ± 0.71
4.556GlyGlu: 4.556 ± 0.865
4.719GlyPhe: 4.719 ± 0.796
6.02GlyGly: 6.02 ± 1.586
0.651GlyHis: 0.651 ± 0.399
4.719GlyIle: 4.719 ± 0.923
5.369GlyLys: 5.369 ± 0.889
4.719GlyLeu: 4.719 ± 0.727
1.464GlyMet: 1.464 ± 0.366
4.881GlyAsn: 4.881 ± 1.279
0.0GlyPro: 0.0 ± 0.0
2.115GlyGln: 2.115 ± 0.422
2.929GlyArg: 2.929 ± 0.683
3.417GlySer: 3.417 ± 0.923
3.58GlyThr: 3.58 ± 0.637
3.742GlyVal: 3.742 ± 0.538
0.976GlyTrp: 0.976 ± 0.482
2.603GlyTyr: 2.603 ± 0.689
0.0GlyXaa: 0.0 ± 0.0
His
0.976HisAla: 0.976 ± 0.382
0.163HisCys: 0.163 ± 0.132
0.814HisAsp: 0.814 ± 0.329
0.976HisGlu: 0.976 ± 0.458
0.814HisPhe: 0.814 ± 0.368
1.627HisGly: 1.627 ± 0.548
0.488HisHis: 0.488 ± 0.221
1.464HisIle: 1.464 ± 0.429
1.139HisLys: 1.139 ± 0.438
1.139HisLeu: 1.139 ± 0.479
0.325HisMet: 0.325 ± 0.218
1.139HisAsn: 1.139 ± 0.383
0.325HisPro: 0.325 ± 0.249
0.488HisGln: 0.488 ± 0.245
0.651HisArg: 0.651 ± 0.273
0.976HisSer: 0.976 ± 0.397
1.139HisThr: 1.139 ± 0.411
1.139HisVal: 1.139 ± 0.506
0.163HisTrp: 0.163 ± 0.19
1.139HisTyr: 1.139 ± 0.453
0.0HisXaa: 0.0 ± 0.0
Ile
2.929IleAla: 2.929 ± 0.578
0.651IleCys: 0.651 ± 0.354
5.207IleAsp: 5.207 ± 0.996
5.207IleGlu: 5.207 ± 1.049
1.79IlePhe: 1.79 ± 0.625
3.905IleGly: 3.905 ± 0.766
1.139IleHis: 1.139 ± 0.547
4.068IleIle: 4.068 ± 0.97
5.857IleLys: 5.857 ± 1.103
4.719IleLeu: 4.719 ± 0.944
1.464IleMet: 1.464 ± 0.517
4.719IleAsn: 4.719 ± 0.691
2.115IlePro: 2.115 ± 0.679
3.417IleGln: 3.417 ± 0.657
2.603IleArg: 2.603 ± 0.542
4.068IleSer: 4.068 ± 0.884
4.393IleThr: 4.393 ± 0.9
4.068IleVal: 4.068 ± 0.765
0.651IleTrp: 0.651 ± 0.245
4.881IleTyr: 4.881 ± 0.839
0.0IleXaa: 0.0 ± 0.0
Lys
4.23LysAla: 4.23 ± 0.973
0.0LysCys: 0.0 ± 0.0
4.393LysAsp: 4.393 ± 0.882
6.346LysGlu: 6.346 ± 1.178
3.091LysPhe: 3.091 ± 0.691
5.695LysGly: 5.695 ± 1.061
1.952LysHis: 1.952 ± 0.471
4.068LysIle: 4.068 ± 0.663
6.02LysLys: 6.02 ± 1.525
7.322LysLeu: 7.322 ± 0.976
3.417LysMet: 3.417 ± 0.852
4.068LysAsn: 4.068 ± 0.824
1.302LysPro: 1.302 ± 0.458
2.929LysGln: 2.929 ± 0.577
3.254LysArg: 3.254 ± 0.61
4.23LysSer: 4.23 ± 1.04
4.068LysThr: 4.068 ± 0.944
5.532LysVal: 5.532 ± 0.88
0.976LysTrp: 0.976 ± 0.459
2.766LysTyr: 2.766 ± 0.711
0.0LysXaa: 0.0 ± 0.0
Leu
3.417LeuAla: 3.417 ± 0.673
0.651LeuCys: 0.651 ± 0.29
5.044LeuAsp: 5.044 ± 0.83
5.695LeuGlu: 5.695 ± 1.052
3.254LeuPhe: 3.254 ± 0.751
3.905LeuGly: 3.905 ± 0.848
1.79LeuHis: 1.79 ± 0.453
4.393LeuIle: 4.393 ± 0.851
7.485LeuLys: 7.485 ± 0.961
5.857LeuLeu: 5.857 ± 0.84
2.603LeuMet: 2.603 ± 0.664
5.695LeuAsn: 5.695 ± 0.785
3.254LeuPro: 3.254 ± 0.763
3.091LeuGln: 3.091 ± 0.655
3.254LeuArg: 3.254 ± 0.698
6.183LeuSer: 6.183 ± 1.091
6.02LeuThr: 6.02 ± 0.783
4.719LeuVal: 4.719 ± 0.837
1.139LeuTrp: 1.139 ± 0.347
4.068LeuTyr: 4.068 ± 0.813
0.0LeuXaa: 0.0 ± 0.0
Met
1.627MetAla: 1.627 ± 0.551
0.0MetCys: 0.0 ± 0.0
2.441MetAsp: 2.441 ± 0.598
1.952MetGlu: 1.952 ± 0.488
1.627MetPhe: 1.627 ± 0.568
1.464MetGly: 1.464 ± 0.402
0.651MetHis: 0.651 ± 0.406
2.441MetIle: 2.441 ± 0.56
0.976MetLys: 0.976 ± 0.393
2.766MetLeu: 2.766 ± 0.587
0.651MetMet: 0.651 ± 0.372
0.976MetAsn: 0.976 ± 0.359
1.139MetPro: 1.139 ± 0.409
0.325MetGln: 0.325 ± 0.24
1.139MetArg: 1.139 ± 0.363
2.278MetSer: 2.278 ± 0.494
2.115MetThr: 2.115 ± 0.456
1.464MetVal: 1.464 ± 0.471
0.0MetTrp: 0.0 ± 0.0
0.976MetTyr: 0.976 ± 0.362
0.0MetXaa: 0.0 ± 0.0
Asn
5.532AsnAla: 5.532 ± 1.312
0.651AsnCys: 0.651 ± 0.418
3.417AsnAsp: 3.417 ± 0.565
5.044AsnGlu: 5.044 ± 0.822
1.464AsnPhe: 1.464 ± 0.631
4.23AsnGly: 4.23 ± 0.915
0.325AsnHis: 0.325 ± 0.189
4.393AsnIle: 4.393 ± 0.836
4.556AsnLys: 4.556 ± 0.763
5.695AsnLeu: 5.695 ± 1.116
0.814AsnMet: 0.814 ± 0.349
4.23AsnAsn: 4.23 ± 0.856
2.441AsnPro: 2.441 ± 0.906
2.441AsnGln: 2.441 ± 0.529
4.068AsnArg: 4.068 ± 0.817
4.393AsnSer: 4.393 ± 0.808
3.417AsnThr: 3.417 ± 0.823
5.207AsnVal: 5.207 ± 0.945
0.488AsnTrp: 0.488 ± 0.263
3.417AsnTyr: 3.417 ± 0.843
0.0AsnXaa: 0.0 ± 0.0
Pro
1.139ProAla: 1.139 ± 0.562
0.163ProCys: 0.163 ± 0.142
1.464ProAsp: 1.464 ± 0.404
2.441ProGlu: 2.441 ± 0.735
0.976ProPhe: 0.976 ± 0.356
0.163ProGly: 0.163 ± 0.19
0.163ProHis: 0.163 ± 0.151
1.464ProIle: 1.464 ± 0.357
2.278ProLys: 2.278 ± 0.652
2.441ProLeu: 2.441 ± 0.438
1.302ProMet: 1.302 ± 0.393
2.603ProAsn: 2.603 ± 0.954
0.814ProPro: 0.814 ± 0.284
0.651ProGln: 0.651 ± 0.247
0.651ProArg: 0.651 ± 0.478
1.139ProSer: 1.139 ± 0.36
1.79ProThr: 1.79 ± 0.599
2.603ProVal: 2.603 ± 0.629
0.488ProTrp: 0.488 ± 0.257
1.627ProTyr: 1.627 ± 0.413
0.0ProXaa: 0.0 ± 0.0
Gln
2.278GlnAla: 2.278 ± 0.485
0.325GlnCys: 0.325 ± 0.248
2.115GlnAsp: 2.115 ± 0.455
2.441GlnGlu: 2.441 ± 0.722
0.814GlnPhe: 0.814 ± 0.416
2.766GlnGly: 2.766 ± 0.656
0.488GlnHis: 0.488 ± 0.381
2.929GlnIle: 2.929 ± 0.577
2.603GlnLys: 2.603 ± 0.626
3.417GlnLeu: 3.417 ± 0.714
0.651GlnMet: 0.651 ± 0.342
0.976GlnAsn: 0.976 ± 0.352
1.464GlnPro: 1.464 ± 0.472
1.139GlnGln: 1.139 ± 0.331
2.115GlnArg: 2.115 ± 0.772
1.302GlnSer: 1.302 ± 0.422
1.464GlnThr: 1.464 ± 0.487
3.58GlnVal: 3.58 ± 0.649
0.976GlnTrp: 0.976 ± 0.427
0.814GlnTyr: 0.814 ± 0.276
0.0GlnXaa: 0.0 ± 0.0
Arg
2.278ArgAla: 2.278 ± 0.725
0.163ArgCys: 0.163 ± 0.142
1.79ArgAsp: 1.79 ± 0.486
4.23ArgGlu: 4.23 ± 0.451
3.254ArgPhe: 3.254 ± 0.628
2.441ArgGly: 2.441 ± 0.794
0.814ArgHis: 0.814 ± 0.305
2.441ArgIle: 2.441 ± 0.572
2.766ArgLys: 2.766 ± 0.735
4.068ArgLeu: 4.068 ± 0.808
1.139ArgMet: 1.139 ± 0.391
2.929ArgAsn: 2.929 ± 0.912
0.976ArgPro: 0.976 ± 0.3
2.115ArgGln: 2.115 ± 0.401
1.302ArgArg: 1.302 ± 0.484
2.278ArgSer: 2.278 ± 0.876
2.766ArgThr: 2.766 ± 0.529
2.278ArgVal: 2.278 ± 0.532
0.325ArgTrp: 0.325 ± 0.274
1.952ArgTyr: 1.952 ± 0.498
0.0ArgXaa: 0.0 ± 0.0
Ser
4.068SerAla: 4.068 ± 0.989
0.488SerCys: 0.488 ± 0.249
3.091SerAsp: 3.091 ± 0.688
3.905SerGlu: 3.905 ± 0.934
2.278SerPhe: 2.278 ± 0.447
3.742SerGly: 3.742 ± 0.688
1.464SerHis: 1.464 ± 0.517
4.719SerIle: 4.719 ± 0.868
3.742SerLys: 3.742 ± 0.945
4.881SerLeu: 4.881 ± 0.99
1.627SerMet: 1.627 ± 0.508
2.603SerAsn: 2.603 ± 0.644
2.278SerPro: 2.278 ± 0.56
1.79SerGln: 1.79 ± 0.553
2.278SerArg: 2.278 ± 0.614
3.091SerSer: 3.091 ± 0.712
4.068SerThr: 4.068 ± 0.784
3.742SerVal: 3.742 ± 1.15
0.0SerTrp: 0.0 ± 0.0
3.254SerTyr: 3.254 ± 0.881
0.0SerXaa: 0.0 ± 0.0
Thr
3.091ThrAla: 3.091 ± 0.642
0.325ThrCys: 0.325 ± 0.38
4.393ThrAsp: 4.393 ± 0.765
4.556ThrGlu: 4.556 ± 0.709
2.929ThrPhe: 2.929 ± 0.74
3.254ThrGly: 3.254 ± 0.578
0.651ThrHis: 0.651 ± 0.304
6.996ThrIle: 6.996 ± 1.068
5.207ThrLys: 5.207 ± 0.757
4.393ThrLeu: 4.393 ± 0.899
1.139ThrMet: 1.139 ± 0.456
4.393ThrAsn: 4.393 ± 0.812
2.115ThrPro: 2.115 ± 0.536
1.952ThrGln: 1.952 ± 0.609
4.393ThrArg: 4.393 ± 0.76
4.068ThrSer: 4.068 ± 0.956
2.766ThrThr: 2.766 ± 0.789
3.742ThrVal: 3.742 ± 0.765
0.651ThrTrp: 0.651 ± 0.297
1.952ThrTyr: 1.952 ± 0.409
0.0ThrXaa: 0.0 ± 0.0
Val
2.766ValAla: 2.766 ± 0.652
0.814ValCys: 0.814 ± 0.457
5.044ValAsp: 5.044 ± 1.076
7.647ValGlu: 7.647 ± 1.171
3.417ValPhe: 3.417 ± 0.705
3.58ValGly: 3.58 ± 0.604
0.976ValHis: 0.976 ± 0.329
4.393ValIle: 4.393 ± 0.652
4.556ValLys: 4.556 ± 0.963
6.346ValLeu: 6.346 ± 1.04
1.627ValMet: 1.627 ± 0.403
4.881ValAsn: 4.881 ± 0.835
0.651ValPro: 0.651 ± 0.305
1.952ValGln: 1.952 ± 0.759
2.929ValArg: 2.929 ± 0.546
3.58ValSer: 3.58 ± 0.709
4.719ValThr: 4.719 ± 0.789
5.857ValVal: 5.857 ± 0.827
0.488ValTrp: 0.488 ± 0.24
3.905ValTyr: 3.905 ± 0.711
0.0ValXaa: 0.0 ± 0.0
Trp
0.651TrpAla: 0.651 ± 0.34
0.0TrpCys: 0.0 ± 0.0
0.163TrpAsp: 0.163 ± 0.142
0.814TrpGlu: 0.814 ± 0.443
0.651TrpPhe: 0.651 ± 0.328
0.488TrpGly: 0.488 ± 0.233
0.488TrpHis: 0.488 ± 0.224
0.163TrpIle: 0.163 ± 0.19
1.139TrpLys: 1.139 ± 0.384
1.302TrpLeu: 1.302 ± 0.466
0.651TrpMet: 0.651 ± 0.285
0.814TrpAsn: 0.814 ± 0.349
0.0TrpPro: 0.0 ± 0.0
0.488TrpGln: 0.488 ± 0.228
0.0TrpArg: 0.0 ± 0.0
0.814TrpSer: 0.814 ± 0.342
0.814TrpThr: 0.814 ± 0.582
0.814TrpVal: 0.814 ± 0.321
0.0TrpTrp: 0.0 ± 0.0
0.325TrpTyr: 0.325 ± 0.224
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.766TyrAla: 2.766 ± 0.715
0.488TyrCys: 0.488 ± 0.263
3.091TyrAsp: 3.091 ± 0.674
3.091TyrGlu: 3.091 ± 0.812
3.091TyrPhe: 3.091 ± 0.72
2.929TyrGly: 2.929 ± 0.612
0.325TyrHis: 0.325 ± 0.171
3.58TyrIle: 3.58 ± 0.8
4.556TyrLys: 4.556 ± 0.777
3.742TyrLeu: 3.742 ± 0.703
0.814TyrMet: 0.814 ± 0.336
3.742TyrAsn: 3.742 ± 0.847
2.603TyrPro: 2.603 ± 0.757
2.115TyrGln: 2.115 ± 0.619
0.651TyrArg: 0.651 ± 0.33
1.627TyrSer: 1.627 ± 0.52
2.441TyrThr: 2.441 ± 0.615
3.091TyrVal: 3.091 ± 0.599
0.814TyrTrp: 0.814 ± 0.308
1.627TyrTyr: 1.627 ± 0.407
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 31 proteins (6147 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski