Amino acid dipepetide frequency for Enterobacteria phage ST104

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.582AlaAla: 9.582 ± 1.287
1.505AlaCys: 1.505 ± 0.366
6.494AlaAsp: 6.494 ± 1.073
7.127AlaGlu: 7.127 ± 0.783
3.801AlaPhe: 3.801 ± 0.58
6.335AlaGly: 6.335 ± 0.863
1.188AlaHis: 1.188 ± 0.275
5.702AlaIle: 5.702 ± 0.745
5.068AlaLys: 5.068 ± 0.609
7.602AlaLeu: 7.602 ± 1.0
3.801AlaMet: 3.801 ± 0.669
7.285AlaAsn: 7.285 ± 1.259
2.217AlaPro: 2.217 ± 0.358
3.959AlaGln: 3.959 ± 0.901
5.385AlaArg: 5.385 ± 0.732
5.464AlaSer: 5.464 ± 0.813
5.939AlaThr: 5.939 ± 0.836
4.91AlaVal: 4.91 ± 0.511
1.505AlaTrp: 1.505 ± 0.332
2.296AlaTyr: 2.296 ± 0.404
0.0AlaXaa: 0.0 ± 0.0
Cys
0.871CysAla: 0.871 ± 0.307
0.317CysCys: 0.317 ± 0.151
0.475CysAsp: 0.475 ± 0.191
0.792CysGlu: 0.792 ± 0.279
0.554CysPhe: 0.554 ± 0.204
1.584CysGly: 1.584 ± 0.407
0.396CysHis: 0.396 ± 0.17
0.554CysIle: 0.554 ± 0.218
1.029CysLys: 1.029 ± 0.328
0.396CysLeu: 0.396 ± 0.194
0.238CysMet: 0.238 ± 0.136
0.792CysAsn: 0.792 ± 0.207
0.317CysPro: 0.317 ± 0.132
0.475CysGln: 0.475 ± 0.155
1.425CysArg: 1.425 ± 0.363
1.188CysSer: 1.188 ± 0.298
0.396CysThr: 0.396 ± 0.191
1.109CysVal: 1.109 ± 0.293
0.079CysTrp: 0.079 ± 0.079
0.554CysTyr: 0.554 ± 0.191
0.0CysXaa: 0.0 ± 0.0
Asp
7.127AspAla: 7.127 ± 0.871
0.317AspCys: 0.317 ± 0.18
3.643AspAsp: 3.643 ± 0.575
4.118AspGlu: 4.118 ± 0.587
1.821AspPhe: 1.821 ± 0.297
5.781AspGly: 5.781 ± 0.871
1.029AspHis: 1.029 ± 0.349
3.801AspIle: 3.801 ± 0.604
3.009AspLys: 3.009 ± 0.475
5.226AspLeu: 5.226 ± 1.059
1.029AspMet: 1.029 ± 0.313
2.217AspAsn: 2.217 ± 0.418
1.584AspPro: 1.584 ± 0.343
1.584AspGln: 1.584 ± 0.288
1.98AspArg: 1.98 ± 0.312
3.009AspSer: 3.009 ± 0.52
2.296AspThr: 2.296 ± 0.417
4.831AspVal: 4.831 ± 0.618
0.95AspTrp: 0.95 ± 0.323
2.692AspTyr: 2.692 ± 0.455
0.0AspXaa: 0.0 ± 0.0
Glu
5.622GluAla: 5.622 ± 0.622
1.505GluCys: 1.505 ± 0.38
2.772GluAsp: 2.772 ± 0.478
4.355GluGlu: 4.355 ± 0.785
1.821GluPhe: 1.821 ± 0.367
3.088GluGly: 3.088 ± 0.478
1.029GluHis: 1.029 ± 0.267
3.405GluIle: 3.405 ± 0.469
4.118GluLys: 4.118 ± 0.524
4.989GluLeu: 4.989 ± 0.602
2.613GluMet: 2.613 ± 0.447
2.93GluAsn: 2.93 ± 0.473
2.455GluPro: 2.455 ± 0.387
3.484GluGln: 3.484 ± 0.631
4.435GluArg: 4.435 ± 0.678
3.643GluSer: 3.643 ± 0.516
3.088GluThr: 3.088 ± 0.484
3.959GluVal: 3.959 ± 0.484
1.663GluTrp: 1.663 ± 0.38
2.296GluTyr: 2.296 ± 0.551
0.0GluXaa: 0.0 ± 0.0
Phe
3.009PheAla: 3.009 ± 0.394
0.554PheCys: 0.554 ± 0.193
1.901PheAsp: 1.901 ± 0.412
1.98PheGlu: 1.98 ± 0.39
1.505PhePhe: 1.505 ± 0.478
2.376PheGly: 2.376 ± 0.352
0.634PheHis: 0.634 ± 0.239
2.613PheIle: 2.613 ± 0.663
1.901PheLys: 1.901 ± 0.368
2.692PheLeu: 2.692 ± 0.448
1.029PheMet: 1.029 ± 0.224
2.059PheAsn: 2.059 ± 0.397
1.109PhePro: 1.109 ± 0.284
1.188PheGln: 1.188 ± 0.337
1.505PheArg: 1.505 ± 0.252
2.772PheSer: 2.772 ± 0.417
1.98PheThr: 1.98 ± 0.416
1.821PheVal: 1.821 ± 0.314
0.475PheTrp: 0.475 ± 0.174
1.98PheTyr: 1.98 ± 0.419
0.0PheXaa: 0.0 ± 0.0
Gly
7.365GlyAla: 7.365 ± 1.577
0.713GlyCys: 0.713 ± 0.212
4.118GlyAsp: 4.118 ± 0.546
4.039GlyGlu: 4.039 ± 0.494
2.851GlyPhe: 2.851 ± 0.469
5.226GlyGly: 5.226 ± 0.906
0.713GlyHis: 0.713 ± 0.239
4.514GlyIle: 4.514 ± 0.591
4.514GlyLys: 4.514 ± 0.63
5.068GlyLeu: 5.068 ± 0.719
2.376GlyMet: 2.376 ± 0.392
3.168GlyAsn: 3.168 ± 0.406
0.95GlyPro: 0.95 ± 0.238
3.484GlyGln: 3.484 ± 0.726
5.068GlyArg: 5.068 ± 0.722
4.435GlySer: 4.435 ± 0.763
3.722GlyThr: 3.722 ± 0.499
6.731GlyVal: 6.731 ± 0.746
1.505GlyTrp: 1.505 ± 0.333
2.059GlyTyr: 2.059 ± 0.373
0.0GlyXaa: 0.0 ± 0.0
His
0.792HisAla: 0.792 ± 0.211
0.475HisCys: 0.475 ± 0.208
0.871HisAsp: 0.871 ± 0.311
1.267HisGlu: 1.267 ± 0.374
0.713HisPhe: 0.713 ± 0.226
1.267HisGly: 1.267 ± 0.32
0.396HisHis: 0.396 ± 0.162
0.554HisIle: 0.554 ± 0.16
0.871HisLys: 0.871 ± 0.271
1.821HisLeu: 1.821 ± 0.457
0.475HisMet: 0.475 ± 0.187
0.396HisAsn: 0.396 ± 0.165
1.188HisPro: 1.188 ± 0.29
0.871HisGln: 0.871 ± 0.254
0.871HisArg: 0.871 ± 0.23
0.713HisSer: 0.713 ± 0.241
0.871HisThr: 0.871 ± 0.231
0.95HisVal: 0.95 ± 0.272
0.238HisTrp: 0.238 ± 0.121
0.554HisTyr: 0.554 ± 0.197
0.0HisXaa: 0.0 ± 0.0
Ile
5.781IleAla: 5.781 ± 0.646
0.713IleCys: 0.713 ± 0.217
3.326IleAsp: 3.326 ± 0.439
4.118IleGlu: 4.118 ± 0.649
1.584IlePhe: 1.584 ± 0.511
4.355IleGly: 4.355 ± 0.695
1.188IleHis: 1.188 ± 0.258
4.514IleIle: 4.514 ± 0.865
3.247IleLys: 3.247 ± 0.547
3.247IleLeu: 3.247 ± 0.667
1.188IleMet: 1.188 ± 0.313
3.088IleAsn: 3.088 ± 0.575
2.851IlePro: 2.851 ± 0.506
2.534IleGln: 2.534 ± 0.401
3.722IleArg: 3.722 ± 0.463
4.989IleSer: 4.989 ± 0.707
3.88IleThr: 3.88 ± 0.571
3.247IleVal: 3.247 ± 0.501
0.475IleTrp: 0.475 ± 0.173
1.663IleTyr: 1.663 ± 0.387
0.0IleXaa: 0.0 ± 0.0
Lys
6.573LysAla: 6.573 ± 0.837
0.792LysCys: 0.792 ± 0.288
3.326LysAsp: 3.326 ± 0.489
3.88LysGlu: 3.88 ± 0.556
1.901LysPhe: 1.901 ± 0.412
4.593LysGly: 4.593 ± 0.535
0.713LysHis: 0.713 ± 0.255
3.405LysIle: 3.405 ± 0.48
3.643LysLys: 3.643 ± 0.528
4.989LysLeu: 4.989 ± 0.693
1.663LysMet: 1.663 ± 0.374
2.059LysAsn: 2.059 ± 0.429
3.643LysPro: 3.643 ± 0.583
3.88LysGln: 3.88 ± 0.552
4.118LysArg: 4.118 ± 0.676
3.484LysSer: 3.484 ± 0.529
3.564LysThr: 3.564 ± 0.558
2.376LysVal: 2.376 ± 0.507
0.713LysTrp: 0.713 ± 0.248
2.217LysTyr: 2.217 ± 0.419
0.0LysXaa: 0.0 ± 0.0
Leu
8.077LeuAla: 8.077 ± 0.788
1.425LeuCys: 1.425 ± 0.381
3.722LeuAsp: 3.722 ± 0.582
4.672LeuGlu: 4.672 ± 0.512
2.534LeuPhe: 2.534 ± 0.541
4.514LeuGly: 4.514 ± 0.804
1.109LeuHis: 1.109 ± 0.277
4.831LeuIle: 4.831 ± 0.839
5.464LeuLys: 5.464 ± 0.705
5.781LeuLeu: 5.781 ± 0.648
2.138LeuMet: 2.138 ± 0.378
3.88LeuAsn: 3.88 ± 0.617
3.484LeuPro: 3.484 ± 0.534
3.088LeuGln: 3.088 ± 0.594
5.068LeuArg: 5.068 ± 0.781
5.226LeuSer: 5.226 ± 0.643
5.068LeuThr: 5.068 ± 0.519
3.564LeuVal: 3.564 ± 0.496
0.95LeuTrp: 0.95 ± 0.28
3.247LeuTyr: 3.247 ± 0.778
0.0LeuXaa: 0.0 ± 0.0
Met
3.009MetAla: 3.009 ± 0.437
0.238MetCys: 0.238 ± 0.109
1.505MetAsp: 1.505 ± 0.34
1.267MetGlu: 1.267 ± 0.267
0.713MetPhe: 0.713 ± 0.285
1.821MetGly: 1.821 ± 0.362
0.079MetHis: 0.079 ± 0.08
1.188MetIle: 1.188 ± 0.307
2.217MetLys: 2.217 ± 0.472
1.742MetLeu: 1.742 ± 0.296
1.109MetMet: 1.109 ± 0.321
1.425MetAsn: 1.425 ± 0.401
1.188MetPro: 1.188 ± 0.288
1.109MetGln: 1.109 ± 0.329
2.059MetArg: 2.059 ± 0.423
1.901MetSer: 1.901 ± 0.37
2.296MetThr: 2.296 ± 0.41
1.505MetVal: 1.505 ± 0.294
0.238MetTrp: 0.238 ± 0.145
1.188MetTyr: 1.188 ± 0.321
0.0MetXaa: 0.0 ± 0.0
Asn
5.068AsnAla: 5.068 ± 0.713
0.238AsnCys: 0.238 ± 0.138
2.613AsnAsp: 2.613 ± 0.407
3.326AsnGlu: 3.326 ± 0.456
0.792AsnPhe: 0.792 ± 0.242
3.959AsnGly: 3.959 ± 0.583
1.029AsnHis: 1.029 ± 0.322
2.772AsnIle: 2.772 ± 0.421
2.93AsnLys: 2.93 ± 0.395
4.039AsnLeu: 4.039 ± 1.109
1.346AsnMet: 1.346 ± 0.342
2.455AsnAsn: 2.455 ± 0.471
2.217AsnPro: 2.217 ± 0.383
2.772AsnGln: 2.772 ± 0.515
2.217AsnArg: 2.217 ± 0.411
2.376AsnSer: 2.376 ± 0.539
2.851AsnThr: 2.851 ± 0.483
3.168AsnVal: 3.168 ± 0.743
0.554AsnTrp: 0.554 ± 0.215
1.188AsnTyr: 1.188 ± 0.317
0.0AsnXaa: 0.0 ± 0.0
Pro
3.168ProAla: 3.168 ± 0.58
0.079ProCys: 0.079 ± 0.084
3.247ProAsp: 3.247 ± 0.492
4.197ProGlu: 4.197 ± 0.579
1.584ProPhe: 1.584 ± 0.357
2.138ProGly: 2.138 ± 0.426
0.634ProHis: 0.634 ± 0.232
2.534ProIle: 2.534 ± 0.497
2.376ProLys: 2.376 ± 0.459
2.692ProLeu: 2.692 ± 0.539
0.634ProMet: 0.634 ± 0.18
1.584ProAsn: 1.584 ± 0.366
1.663ProPro: 1.663 ± 0.373
1.901ProGln: 1.901 ± 0.344
1.663ProArg: 1.663 ± 0.315
2.613ProSer: 2.613 ± 0.396
1.98ProThr: 1.98 ± 0.328
2.93ProVal: 2.93 ± 0.498
0.396ProTrp: 0.396 ± 0.182
1.346ProTyr: 1.346 ± 0.352
0.0ProXaa: 0.0 ± 0.0
Gln
4.355GlnAla: 4.355 ± 0.862
0.713GlnCys: 0.713 ± 0.227
2.692GlnAsp: 2.692 ± 0.417
2.138GlnGlu: 2.138 ± 0.461
1.584GlnPhe: 1.584 ± 0.358
3.168GlnGly: 3.168 ± 0.606
0.713GlnHis: 0.713 ± 0.192
2.851GlnIle: 2.851 ± 0.464
2.376GlnLys: 2.376 ± 0.507
4.593GlnLeu: 4.593 ± 0.525
1.584GlnMet: 1.584 ± 0.344
2.138GlnAsn: 2.138 ± 0.664
2.376GlnPro: 2.376 ± 0.494
3.564GlnGln: 3.564 ± 0.933
2.376GlnArg: 2.376 ± 0.399
2.613GlnSer: 2.613 ± 0.464
1.584GlnThr: 1.584 ± 0.406
2.296GlnVal: 2.296 ± 0.448
1.109GlnTrp: 1.109 ± 0.304
1.98GlnTyr: 1.98 ± 0.37
0.0GlnXaa: 0.0 ± 0.0
Arg
4.593ArgAla: 4.593 ± 0.678
0.396ArgCys: 0.396 ± 0.232
4.039ArgAsp: 4.039 ± 0.671
4.593ArgGlu: 4.593 ± 0.703
2.217ArgPhe: 2.217 ± 0.47
3.801ArgGly: 3.801 ± 0.659
1.346ArgHis: 1.346 ± 0.324
3.88ArgIle: 3.88 ± 0.567
4.435ArgLys: 4.435 ± 0.677
5.385ArgLeu: 5.385 ± 0.645
1.901ArgMet: 1.901 ± 0.383
3.088ArgAsn: 3.088 ± 0.537
1.821ArgPro: 1.821 ± 0.337
2.455ArgGln: 2.455 ± 0.47
4.039ArgArg: 4.039 ± 0.839
3.564ArgSer: 3.564 ± 0.491
1.663ArgThr: 1.663 ± 0.375
3.247ArgVal: 3.247 ± 0.47
1.109ArgTrp: 1.109 ± 0.299
1.98ArgTyr: 1.98 ± 0.444
0.0ArgXaa: 0.0 ± 0.0
Ser
6.177SerAla: 6.177 ± 0.996
0.95SerCys: 0.95 ± 0.335
3.088SerAsp: 3.088 ± 0.399
2.851SerGlu: 2.851 ± 0.478
2.534SerPhe: 2.534 ± 0.516
5.622SerGly: 5.622 ± 0.815
1.346SerHis: 1.346 ± 0.302
3.168SerIle: 3.168 ± 0.509
3.484SerLys: 3.484 ± 0.433
5.306SerLeu: 5.306 ± 0.644
1.663SerMet: 1.663 ± 0.288
1.98SerAsn: 1.98 ± 0.294
3.009SerPro: 3.009 ± 0.555
3.088SerGln: 3.088 ± 0.486
3.564SerArg: 3.564 ± 0.482
3.088SerSer: 3.088 ± 0.691
3.088SerThr: 3.088 ± 0.588
3.326SerVal: 3.326 ± 0.441
0.95SerTrp: 0.95 ± 0.269
1.98SerTyr: 1.98 ± 0.462
0.0SerXaa: 0.0 ± 0.0
Thr
5.543ThrAla: 5.543 ± 0.737
0.396ThrCys: 0.396 ± 0.159
3.009ThrAsp: 3.009 ± 0.549
2.534ThrGlu: 2.534 ± 0.494
2.059ThrPhe: 2.059 ± 0.504
4.672ThrGly: 4.672 ± 0.671
1.029ThrHis: 1.029 ± 0.226
3.405ThrIle: 3.405 ± 0.485
4.039ThrLys: 4.039 ± 0.53
4.276ThrLeu: 4.276 ± 0.571
0.634ThrMet: 0.634 ± 0.213
2.217ThrAsn: 2.217 ± 0.498
3.326ThrPro: 3.326 ± 0.565
2.217ThrGln: 2.217 ± 0.434
2.059ThrArg: 2.059 ± 0.402
2.772ThrSer: 2.772 ± 0.541
3.326ThrThr: 3.326 ± 0.605
3.088ThrVal: 3.088 ± 0.479
0.554ThrTrp: 0.554 ± 0.192
1.821ThrTyr: 1.821 ± 0.319
0.0ThrXaa: 0.0 ± 0.0
Val
6.098ValAla: 6.098 ± 0.638
1.029ValCys: 1.029 ± 0.324
3.009ValAsp: 3.009 ± 0.389
3.722ValGlu: 3.722 ± 0.419
2.534ValPhe: 2.534 ± 0.459
4.355ValGly: 4.355 ± 0.578
0.554ValHis: 0.554 ± 0.205
3.405ValIle: 3.405 ± 0.518
4.118ValLys: 4.118 ± 0.728
4.197ValLeu: 4.197 ± 0.567
1.109ValMet: 1.109 ± 0.335
3.484ValAsn: 3.484 ± 0.632
1.742ValPro: 1.742 ± 0.39
2.376ValGln: 2.376 ± 0.472
3.564ValArg: 3.564 ± 0.391
3.88ValSer: 3.88 ± 0.676
3.247ValThr: 3.247 ± 0.463
4.91ValVal: 4.91 ± 0.617
0.95ValTrp: 0.95 ± 0.251
2.217ValTyr: 2.217 ± 0.504
0.0ValXaa: 0.0 ± 0.0
Trp
1.346TrpAla: 1.346 ± 0.314
0.396TrpCys: 0.396 ± 0.184
1.346TrpAsp: 1.346 ± 0.29
0.396TrpGlu: 0.396 ± 0.185
0.554TrpPhe: 0.554 ± 0.213
1.029TrpGly: 1.029 ± 0.268
0.475TrpHis: 0.475 ± 0.263
0.713TrpIle: 0.713 ± 0.238
1.188TrpLys: 1.188 ± 0.368
1.267TrpLeu: 1.267 ± 0.363
0.554TrpMet: 0.554 ± 0.188
0.396TrpAsn: 0.396 ± 0.181
0.475TrpPro: 0.475 ± 0.166
0.792TrpGln: 0.792 ± 0.201
1.109TrpArg: 1.109 ± 0.316
0.634TrpSer: 0.634 ± 0.243
0.634TrpThr: 0.634 ± 0.179
1.029TrpVal: 1.029 ± 0.236
0.396TrpTrp: 0.396 ± 0.164
0.554TrpTyr: 0.554 ± 0.237
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.009TyrAla: 3.009 ± 0.495
0.792TyrCys: 0.792 ± 0.233
2.851TyrAsp: 2.851 ± 0.565
1.901TyrGlu: 1.901 ± 0.448
1.425TyrPhe: 1.425 ± 0.292
2.93TyrGly: 2.93 ± 0.895
0.554TyrHis: 0.554 ± 0.217
1.901TyrIle: 1.901 ± 0.448
1.505TyrLys: 1.505 ± 0.323
2.534TyrLeu: 2.534 ± 0.398
0.634TyrMet: 0.634 ± 0.21
1.267TyrAsn: 1.267 ± 0.397
1.742TyrPro: 1.742 ± 0.466
1.98TyrGln: 1.98 ± 0.364
3.326TyrArg: 3.326 ± 0.497
1.901TyrSer: 1.901 ± 0.421
1.584TyrThr: 1.584 ± 0.309
1.505TyrVal: 1.505 ± 0.335
0.475TyrTrp: 0.475 ± 0.209
0.792TyrTyr: 0.792 ± 0.269
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (12629 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski