Triticum urartu (Red wild einkorn) (Crithodium urartu)
Average proteome isoelectric point is 6.74
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 33312 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|M8AIS2|M8AIS2_TRIUA Cucumisin OS=Triticum urartu OX=4572 GN=TRIUR3_23719 PE=3 SV=1
MM1 pKa = 7.76 APTNSPPIHH10 pKa = 6.27 SAQGGAVHH18 pKa = 6.85 GVGNDD23 pKa = 3.34 IVSHH27 pKa = 6.52 ASASLCTAADD37 pKa = 3.7 EE38 pKa = 4.88 ASVFGQGPVDD48 pKa = 3.4 RR49 pKa = 11.84 AVIEE53 pKa = 4.16 MVEE56 pKa = 4.14 VAEE59 pKa = 4.28 NAVVLPQIHH68 pKa = 6.09 NAVEE72 pKa = 4.11 EE73 pKa = 4.23 TGEE76 pKa = 4.62 FDD78 pKa = 4.04 PLDD81 pKa = 3.9 YY82 pKa = 10.9 NAPAGANEE90 pKa = 4.5 LADD93 pKa = 4.18 GDD95 pKa = 4.32 NEE97 pKa = 4.79 SGTGNVDD104 pKa = 3.29 RR105 pKa = 11.84 AAADD109 pKa = 4.15 DD110 pKa = 5.28 DD111 pKa = 4.56 VDD113 pKa = 4.41 SPPSKK118 pKa = 10.84 PEE120 pKa = 3.64 DD121 pKa = 3.29 LVYY124 pKa = 10.72 LFHH127 pKa = 7.15 GSYY130 pKa = 10.87 GVACVV135 pKa = 3.32
Molecular weight: 13.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.723
IPC2_protein 3.846
IPC_protein 3.808
Toseland 3.605
ProMoST 3.973
Dawson 3.795
Bjellqvist 3.961
Wikipedia 3.732
Rodwell 3.643
Grimsley 3.516
Solomon 3.783
Lehninger 3.745
Nozaki 3.923
DTASelect 4.126
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.935
Patrickios 0.947
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.82
Protein with the highest isoelectric point:
>tr|T1LP83|T1LP83_TRIUA Uncharacterized protein OS=Triticum urartu OX=4572 PE=4 SV=1
TT1 pKa = 7.5 AFTPRR6 pKa = 11.84 ATLVSLVVTRR16 pKa = 11.84 TRR18 pKa = 11.84 LPRR21 pKa = 11.84 ATSLRR26 pKa = 11.84 GVSSTSPHH34 pKa = 6.55 ASWLHH39 pKa = 5.06 TSSITRR45 pKa = 11.84 NIFFPVKK52 pKa = 9.36 ASLRR56 pKa = 11.84 IGQSTANTFSLRR68 pKa = 11.84 CFPPARR74 pKa = 11.84 VMAVLRR80 pKa = 11.84 SSTLSKK86 pKa = 10.64 SWLTLNHH93 pKa = 6.36 IFLSKK98 pKa = 10.18 LAFMASSLKK107 pKa = 9.28 TSRR110 pKa = 11.84 ARR112 pKa = 11.84 VVLPIPPTPTMAKK125 pKa = 9.63 TMTLLLSALLVSASGLLTMISTSALVSSSIFSPP158 pKa = 3.74
Molecular weight: 16.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.441
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.369
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.102
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
33312
0
33312
12117229
29
5024
363.7
40.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.321 ± 0.015
2.016 ± 0.007
5.482 ± 0.012
6.11 ± 0.014
3.789 ± 0.008
7.218 ± 0.013
2.562 ± 0.006
4.658 ± 0.01
5.183 ± 0.014
9.791 ± 0.018
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.563 ± 0.005
3.631 ± 0.009
5.209 ± 0.015
3.547 ± 0.011
5.996 ± 0.012
8.053 ± 0.014
4.932 ± 0.009
6.908 ± 0.009
1.355 ± 0.005
2.673 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here