Hubei hepe-like virus 1
Average proteome isoelectric point is 8.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KK13|A0A1L3KK13_9VIRU Uncharacterized protein OS=Hubei hepe-like virus 1 OX=1922894 PE=4 SV=1
MM1 pKa = 7.58 LGLNPASNKK10 pKa = 9.57 TIIDD14 pKa = 3.61 FEE16 pKa = 4.81 KK17 pKa = 10.09 NKK19 pKa = 10.45 AFEE22 pKa = 4.06 FHH24 pKa = 6.96 KK25 pKa = 10.66 KK26 pKa = 10.61 LNGKK30 pKa = 9.72 AIRR33 pKa = 11.84 LPFSLKK39 pKa = 10.73 AQDD42 pKa = 3.96 HH43 pKa = 6.39 SFLSSLYY50 pKa = 9.41 APQPIEE56 pKa = 4.71 LGTARR61 pKa = 11.84 PPSHH65 pKa = 6.84 PVVAALVRR73 pKa = 11.84 IQRR76 pKa = 11.84 DD77 pKa = 3.2 RR78 pKa = 11.84 MNSIARR84 pKa = 11.84 HH85 pKa = 5.55 SGHH88 pKa = 6.2 NVLFFDD94 pKa = 5.16 SSLSPLTDD102 pKa = 3.04 NAAFIANGGNFHH114 pKa = 7.07 LCLNTEE120 pKa = 4.28 DD121 pKa = 4.06 PRR123 pKa = 11.84 YY124 pKa = 9.22 HH125 pKa = 6.18 QRR127 pKa = 11.84 LANALFDD134 pKa = 3.71 HH135 pKa = 6.85 HH136 pKa = 6.44 NQPGFVKK143 pKa = 10.54 DD144 pKa = 3.49 VVQAARR150 pKa = 11.84 LGINTEE156 pKa = 4.35 LVCHH160 pKa = 6.77 RR161 pKa = 11.84 GLHH164 pKa = 5.55 HH165 pKa = 7.24 CNYY168 pKa = 8.45 TSPIAFAPNTISNIDD183 pKa = 3.44 LTTIAKK189 pKa = 9.94 LFYY192 pKa = 10.28 SHH194 pKa = 7.45 GIHH197 pKa = 6.23 VLHH200 pKa = 7.32 AFQIFPALGLVQNRR214 pKa = 11.84 HH215 pKa = 5.8 HH216 pKa = 6.81 YY217 pKa = 10.08 IDD219 pKa = 3.51 YY220 pKa = 10.65 QYY222 pKa = 11.09 TFEE225 pKa = 5.18 IDD227 pKa = 3.29 NDD229 pKa = 3.37 EE230 pKa = 4.23 CLIGNVDD237 pKa = 4.0 DD238 pKa = 4.26 PSYY241 pKa = 10.85 GYY243 pKa = 10.4 RR244 pKa = 11.84 YY245 pKa = 10.15 NWTTFKK251 pKa = 10.99 SHH253 pKa = 7.68 FINSGIVTPFGFNILIEE270 pKa = 4.17 KK271 pKa = 8.18 TRR273 pKa = 11.84 NHH275 pKa = 6.53 GIMWEE280 pKa = 4.13 LKK282 pKa = 7.19 YY283 pKa = 9.59 TRR285 pKa = 11.84 VNGTVDD291 pKa = 3.85 FPFRR295 pKa = 11.84 LPIIPDD301 pKa = 3.18 DD302 pKa = 3.68 TMRR305 pKa = 11.84 FPDD308 pKa = 3.39 ICGVIQKK315 pKa = 10.09 GFYY318 pKa = 10.53 LSGDD322 pKa = 3.79 VVSKK326 pKa = 10.9 YY327 pKa = 10.78 SFSGFDD333 pKa = 3.68 FPPNFPFIVTSKK345 pKa = 10.38 RR346 pKa = 11.84 KK347 pKa = 9.9 CEE349 pKa = 3.62 QLYY352 pKa = 9.31 QFIVTRR358 pKa = 11.84 VEE360 pKa = 3.83 KK361 pKa = 11.02 SFDD364 pKa = 3.8 LEE366 pKa = 3.92 TTAAYY371 pKa = 9.96 ARR373 pKa = 11.84 SLLRR377 pKa = 11.84 RR378 pKa = 11.84 TIVGDD383 pKa = 3.7 HH384 pKa = 6.56 VIEE387 pKa = 4.13 EE388 pKa = 4.19 AWRR391 pKa = 11.84 IDD393 pKa = 3.58 TTEE396 pKa = 4.03 LQHH399 pKa = 6.83 LIVSAYY405 pKa = 8.67 ILAMTSRR412 pKa = 11.84 QVSNQRR418 pKa = 11.84 VSAAVRR424 pKa = 11.84 LLQSRR429 pKa = 11.84 SSIYY433 pKa = 10.66 SGVFNQIGTEE443 pKa = 3.76 ILNLLGSTGARR454 pKa = 11.84 LFPGIPFSFVDD465 pKa = 3.25 RR466 pKa = 11.84 FTRR469 pKa = 11.84 YY470 pKa = 8.12 TEE472 pKa = 4.8 AISGRR477 pKa = 11.84 SNNIFFKK484 pKa = 10.86 LIEE487 pKa = 4.26 NMQSFFDD494 pKa = 3.8 IDD496 pKa = 3.74 HH497 pKa = 6.57 QIHH500 pKa = 6.05 YY501 pKa = 10.02 SAAFDD506 pKa = 3.61 VPFIDD511 pKa = 5.86 KK512 pKa = 10.86 YY513 pKa = 11.06 LLPYY517 pKa = 8.77 PARR520 pKa = 11.84 KK521 pKa = 8.63 IVHH524 pKa = 6.21 GLFHH528 pKa = 7.23 HH529 pKa = 6.44 SRR531 pKa = 11.84 TLATTMMRR539 pKa = 11.84 RR540 pKa = 11.84 FRR542 pKa = 11.84 TLADD546 pKa = 3.24 TTIYY550 pKa = 10.28 HH551 pKa = 5.47 YY552 pKa = 10.84 RR553 pKa = 11.84 QFRR556 pKa = 11.84 LEE558 pKa = 3.81 ATLRR562 pKa = 11.84 CIDD565 pKa = 5.49 IEE567 pKa = 4.25 EE568 pKa = 4.01 QWIGRR573 pKa = 11.84 LLQSYY578 pKa = 11.42 ANIFPPNHH586 pKa = 6.39 PKK588 pKa = 9.98 VVGWRR593 pKa = 11.84 LRR595 pKa = 11.84 SQQWQQRR602 pKa = 11.84 RR603 pKa = 11.84 QVAQDD608 pKa = 3.83 LIARR612 pKa = 11.84 NRR614 pKa = 11.84 KK615 pKa = 8.92 LLISGPNKK623 pKa = 9.81 PLVNLRR629 pKa = 11.84 DD630 pKa = 3.64 PKK632 pKa = 10.64 TRR634 pKa = 11.84 LIDD637 pKa = 3.75 SPPVHH642 pKa = 6.36 PTVITVPLLDD652 pKa = 3.5 QHH654 pKa = 7.01 SKK656 pKa = 10.83 LLSASDD662 pKa = 4.05 KK663 pKa = 10.68 LAQAVDD669 pKa = 3.81 DD670 pKa = 4.4 RR671 pKa = 11.84 LTDD674 pKa = 3.47 ISNNASEE681 pKa = 5.04 IFGSTCDD688 pKa = 3.69 PSFLPQYY695 pKa = 7.84 THH697 pKa = 6.58 NCDD700 pKa = 2.77 HH701 pKa = 7.19 TIYY704 pKa = 10.4 HH705 pKa = 5.53 QVDD708 pKa = 3.77 VPGDD712 pKa = 3.75 GMCAFHH718 pKa = 6.62 TMAHH722 pKa = 6.18 FLGVNATEE730 pKa = 4.06 LRR732 pKa = 11.84 EE733 pKa = 3.99 QIRR736 pKa = 11.84 DD737 pKa = 3.32 SDD739 pKa = 3.73 IAPQQVVDD747 pKa = 5.03 LLSDD751 pKa = 3.85 PDD753 pKa = 3.22 AWGDD757 pKa = 3.75 YY758 pKa = 10.81 PCFAVCAQIYY768 pKa = 8.51 GVRR771 pKa = 11.84 FCIEE775 pKa = 4.05 FVDD778 pKa = 4.01 SRR780 pKa = 11.84 DD781 pKa = 3.51 KK782 pKa = 11.23 IFFLTPGNGPIYY794 pKa = 10.13 HH795 pKa = 6.17 VRR797 pKa = 11.84 LEE799 pKa = 4.51 HH800 pKa = 6.18 NHH802 pKa = 5.86 FSPLEE807 pKa = 3.87 TDD809 pKa = 2.26 IDD811 pKa = 4.25 FPIEE815 pKa = 4.0 GSNVPGIFNLVSSASKK831 pKa = 9.9 PLPSFDD837 pKa = 4.36 YY838 pKa = 11.0 LDD840 pKa = 3.79 PLYY843 pKa = 10.89 RR844 pKa = 11.84 EE845 pKa = 4.77 LKK847 pKa = 9.38 GQNTQLANSITARR860 pKa = 11.84 LNRR863 pKa = 11.84 LKK865 pKa = 9.73 TAKK868 pKa = 9.4 FAKK871 pKa = 10.24 GEE873 pKa = 4.16 IYY875 pKa = 10.5 LGPPGSCKK883 pKa = 9.48 TRR885 pKa = 11.84 ACIKK889 pKa = 10.29 KK890 pKa = 10.1 LAGTGKK896 pKa = 9.99 RR897 pKa = 11.84 VLVSCPTRR905 pKa = 11.84 ALAADD910 pKa = 4.21 WEE912 pKa = 4.83 NKK914 pKa = 8.84 TSTEE918 pKa = 3.88 DD919 pKa = 3.72 FAVLTYY925 pKa = 10.07 QAALVHH931 pKa = 6.44 GFAEE935 pKa = 4.18 QFDD938 pKa = 4.59 TIVVDD943 pKa = 3.78 EE944 pKa = 5.42 CFTIPYY950 pKa = 10.07 SYY952 pKa = 10.89 ILAFAALARR961 pKa = 11.84 VRR963 pKa = 11.84 LILLGDD969 pKa = 3.95 PNQITSIDD977 pKa = 3.73 FSEE980 pKa = 4.9 SKK982 pKa = 10.53 CHH984 pKa = 6.3 TGTPRR989 pKa = 11.84 LEE991 pKa = 5.11 DD992 pKa = 3.87 NLDD995 pKa = 3.76 CYY997 pKa = 11.41 DD998 pKa = 4.75 LYY1000 pKa = 11.43 EE1001 pKa = 4.46 CAATSRR1007 pKa = 11.84 IPEE1010 pKa = 4.91 DD1011 pKa = 3.26 IVEE1014 pKa = 4.22 TDD1016 pKa = 3.73 LFQRR1020 pKa = 11.84 TYY1022 pKa = 10.94 PGISTTNHH1030 pKa = 5.75 RR1031 pKa = 11.84 KK1032 pKa = 9.37 SLHH1035 pKa = 5.49 IHH1037 pKa = 7.26 DD1038 pKa = 4.56 PTAPLPANHH1047 pKa = 5.95 QVLCFTQEE1055 pKa = 3.98 AKK1057 pKa = 10.84 AQLCQFYY1064 pKa = 9.18 PQVKK1068 pKa = 7.81 TVHH1071 pKa = 6.03 EE1072 pKa = 4.38 AQGQTFDD1079 pKa = 4.92 DD1080 pKa = 4.07 VVLVVGSSPGEE1091 pKa = 3.6 LGLAYY1096 pKa = 9.91 EE1097 pKa = 4.92 SYY1099 pKa = 10.32 NHH1101 pKa = 6.71 VIVACSRR1108 pKa = 11.84 HH1109 pKa = 4.48 TQNLVLSIDD1118 pKa = 3.87 DD1119 pKa = 3.48 VATFLQRR1126 pKa = 11.84 TGLQPEE1132 pKa = 4.07 PLVYY1136 pKa = 11.09 VNDD1139 pKa = 3.85 DD1140 pKa = 3.93 VEE1142 pKa = 4.29 LHH1144 pKa = 5.81 TRR1146 pKa = 11.84 DD1147 pKa = 4.88 NIDD1150 pKa = 3.36 DD1151 pKa = 3.85 TPRR1154 pKa = 11.84 IVPADD1159 pKa = 3.89 YY1160 pKa = 11.35 VPFSPPAVEE1169 pKa = 5.19 SFADD1173 pKa = 3.77 NPPSFIKK1180 pKa = 10.73 DD1181 pKa = 3.26 VDD1183 pKa = 3.75 PSSVRR1188 pKa = 11.84 EE1189 pKa = 3.83 VFDD1192 pKa = 3.12 IYY1194 pKa = 11.06 FPVRR1198 pKa = 11.84 EE1199 pKa = 4.29 KK1200 pKa = 10.78 QLEE1203 pKa = 3.99 DD1204 pKa = 3.58 CYY1206 pKa = 11.44 YY1207 pKa = 10.75 ATTTSYY1213 pKa = 9.18 NTEE1216 pKa = 3.81 GSGRR1220 pKa = 11.84 ATVQFEE1226 pKa = 4.27 KK1227 pKa = 10.79 VAEE1230 pKa = 4.09 NSLQEE1235 pKa = 3.92 MRR1237 pKa = 11.84 TFTRR1241 pKa = 11.84 STFSIDD1247 pKa = 2.91 PRR1249 pKa = 11.84 GRR1251 pKa = 11.84 YY1252 pKa = 4.06 TTSRR1256 pKa = 11.84 SRR1258 pKa = 11.84 IQEE1261 pKa = 3.62 VHH1263 pKa = 5.69 TALQRR1268 pKa = 11.84 LLKK1271 pKa = 9.78 FTMNPPRR1278 pKa = 11.84 AYY1280 pKa = 10.44 VLEE1283 pKa = 4.07 HH1284 pKa = 6.91 SIRR1287 pKa = 11.84 LVNQLNDD1294 pKa = 5.36 LIPFCPVQTRR1304 pKa = 11.84 NPEE1307 pKa = 4.71 LIHH1310 pKa = 5.41 QAYY1313 pKa = 9.7 VEE1315 pKa = 4.39 YY1316 pKa = 10.28 ASKK1319 pKa = 8.18 MTEE1322 pKa = 3.63 RR1323 pKa = 11.84 GQGIEE1328 pKa = 5.15 DD1329 pKa = 3.38 ITEE1332 pKa = 3.86 KK1333 pKa = 10.54 TIIDD1337 pKa = 4.38 PGTVSLFLKK1346 pKa = 10.33 QQFKK1350 pKa = 10.68 QDD1352 pKa = 3.68 YY1353 pKa = 8.47 TSGEE1357 pKa = 3.73 GALRR1361 pKa = 11.84 DD1362 pKa = 3.89 KK1363 pKa = 11.02 SGQPISAWDD1372 pKa = 3.32 KK1373 pKa = 7.76 RR1374 pKa = 11.84 TNFIFSVWARR1384 pKa = 11.84 ILEE1387 pKa = 4.38 FYY1389 pKa = 11.01 LLDD1392 pKa = 3.98 SPTPRR1397 pKa = 11.84 FKK1399 pKa = 10.78 FFSRR1403 pKa = 11.84 VSEE1406 pKa = 4.06 PVLLKK1411 pKa = 11.02 CLDD1414 pKa = 3.91 SFPKK1418 pKa = 10.59 DD1419 pKa = 3.47 GISYY1423 pKa = 8.94 FCNDD1427 pKa = 2.64 FTEE1430 pKa = 4.7 YY1431 pKa = 10.89 DD1432 pKa = 3.4 SCQNEE1437 pKa = 4.31 LEE1439 pKa = 4.26 HH1440 pKa = 7.14 EE1441 pKa = 4.18 ILAEE1445 pKa = 3.81 VLRR1448 pKa = 11.84 RR1449 pKa = 11.84 IGAPAALIEE1458 pKa = 4.78 HH1459 pKa = 6.48 YY1460 pKa = 10.89 LHH1462 pKa = 6.74 IRR1464 pKa = 11.84 RR1465 pKa = 11.84 NRR1467 pKa = 11.84 VAYY1470 pKa = 10.05 SQTVKK1475 pKa = 10.85 CKK1477 pKa = 10.53 VKK1479 pKa = 10.43 NKK1481 pKa = 9.49 RR1482 pKa = 11.84 DD1483 pKa = 3.44 SGEE1486 pKa = 3.98 PFTLIGNTIFTSCIVLDD1503 pKa = 4.02 IMDD1506 pKa = 5.33 DD1507 pKa = 3.76 FDD1509 pKa = 4.55 FLLVKK1514 pKa = 10.68 GDD1516 pKa = 3.95 DD1517 pKa = 4.1 SAAGGYY1523 pKa = 9.48 NISVRR1528 pKa = 11.84 HH1529 pKa = 5.64 EE1530 pKa = 4.44 KK1531 pKa = 9.5 IAHH1534 pKa = 4.99 YY1535 pKa = 9.41 FQTNRR1540 pKa = 11.84 YY1541 pKa = 7.21 VMKK1544 pKa = 10.29 PNVSDD1549 pKa = 3.22 SCEE1552 pKa = 3.67 FVGYY1556 pKa = 8.09 ITNVNGTCLDD1566 pKa = 3.23 IVRR1569 pKa = 11.84 LCARR1573 pKa = 11.84 LVGRR1577 pKa = 11.84 KK1578 pKa = 9.06 FKK1580 pKa = 11.07 DD1581 pKa = 3.2 EE1582 pKa = 4.29 EE1583 pKa = 4.29 DD1584 pKa = 3.99 FNDD1587 pKa = 3.55 YY1588 pKa = 10.61 VISVRR1593 pKa = 11.84 DD1594 pKa = 3.48 RR1595 pKa = 11.84 VRR1597 pKa = 11.84 SFASVDD1603 pKa = 3.21 LMHH1606 pKa = 7.24 RR1607 pKa = 11.84 SIAVISLHH1615 pKa = 6.04 YY1616 pKa = 10.79 KK1617 pKa = 8.17 MAPEE1621 pKa = 3.93 HH1622 pKa = 6.79 VEE1624 pKa = 3.38 WLYY1627 pKa = 11.67 NYY1629 pKa = 10.39 LLNFAYY1635 pKa = 10.7 NKK1637 pKa = 10.12 IPFDD1641 pKa = 3.82 ALMSSKK1647 pKa = 10.59 QSIWNLISNIPTRR1660 pKa = 11.84 FF1661 pKa = 3.17
Molecular weight: 189.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.103
IPC2_protein 6.071
IPC_protein 6.16
Toseland 6.504
ProMoST 6.576
Dawson 6.517
Bjellqvist 6.517
Wikipedia 6.532
Rodwell 6.504
Grimsley 6.664
Solomon 6.517
Lehninger 6.517
Nozaki 6.81
DTASelect 6.985
Thurlkill 7.015
EMBOSS 6.971
Sillero 6.912
Patrickios 3.999
IPC_peptide 6.532
IPC2_peptide 6.839
IPC2.peptide.svr19 6.775
Protein with the highest isoelectric point:
>tr|A0A1L3KK49|A0A1L3KK49_9VIRU DiSB-ORF2_chro domain-containing protein OS=Hubei hepe-like virus 1 OX=1922894 PE=4 SV=1
MM1 pKa = 7.24 SFSRR5 pKa = 11.84 ASFRR9 pKa = 11.84 GRR11 pKa = 11.84 FRR13 pKa = 11.84 GRR15 pKa = 11.84 GRR17 pKa = 11.84 AAFMRR22 pKa = 11.84 PPPSYY27 pKa = 10.12 FYY29 pKa = 10.87 YY30 pKa = 10.21 PPPQPLSVPIRR41 pKa = 11.84 PAPPPPTNGNAPKK54 pKa = 10.05 QGYY57 pKa = 9.85 LEE59 pKa = 4.84 TILASCQEE67 pKa = 4.4 NISNPFRR74 pKa = 11.84 LALFILGACFVVAWAINASSPGHH97 pKa = 5.33 QVVQHH102 pKa = 5.12 LRR104 pKa = 11.84 GRR106 pKa = 11.84 LSEE109 pKa = 4.02 THH111 pKa = 4.9 ATKK114 pKa = 10.82 VKK116 pKa = 10.0 TITDD120 pKa = 4.59 FLTTNHH126 pKa = 5.19 VRR128 pKa = 11.84 FFASGVMLIPFSLLYY143 pKa = 10.47 ARR145 pKa = 11.84 QKK147 pKa = 9.07 WLTWALAFLLVIWVAPTYY165 pKa = 10.39 SEE167 pKa = 3.69 WQYY170 pKa = 11.75 VVISLLLFAYY180 pKa = 8.93 YY181 pKa = 10.12 AQKK184 pKa = 9.79 TKK186 pKa = 10.32 EE187 pKa = 3.92 NRR189 pKa = 11.84 QTALVILLVGFVAIHH204 pKa = 5.96 FVV206 pKa = 3.14
Molecular weight: 23.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.308
IPC2_protein 10.043
IPC_protein 10.76
Toseland 10.409
ProMoST 10.262
Dawson 10.643
Bjellqvist 10.394
Wikipedia 10.877
Rodwell 10.804
Grimsley 10.73
Solomon 10.701
Lehninger 10.643
Nozaki 10.423
DTASelect 10.394
Thurlkill 10.482
EMBOSS 10.833
Sillero 10.555
Patrickios 10.423
IPC_peptide 10.687
IPC2_peptide 9.487
IPC2.peptide.svr19 8.141
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2427
206
1661
809.0
92.0
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.18 ± 0.81
1.978 ± 0.169
5.027 ± 1.76
3.832 ± 0.887
6.757 ± 0.591
4.079 ± 0.683
3.708 ± 0.341
6.51 ± 0.563
3.214 ± 0.942
10.919 ± 2.624
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.071 ± 0.073
4.326 ± 0.584
6.634 ± 1.232
3.749 ± 0.555
6.345 ± 0.108
8.241 ± 1.685
6.634 ± 0.533
6.098 ± 0.663
1.03 ± 0.381
3.667 ± 0.442
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here