Kunsagivirus A
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S4VD62|S4VD62_9PICO Capsid protein VP0 OS=Kunsagivirus A OX=1755590 PE=4 SV=1
MM1 pKa = 8.08 DD2 pKa = 6.06 PLSLTAGLVGDD13 pKa = 4.12 VVGGVAGAITGGGDD27 pKa = 3.5 PVGSLLSSGLSLLGLGSSSSTSSPTPSRR55 pKa = 11.84 DD56 pKa = 3.02 GGGPSSIGPQLSAPDD71 pKa = 4.33 LPQPPPSPGLPLSAGGDD88 pKa = 3.88 PQPHH92 pKa = 6.2 GVTSSEE98 pKa = 4.32 PPTPAPTHH106 pKa = 5.07 TQAGSSQSTATPFGGASSGHH126 pKa = 5.7 TPTFEE131 pKa = 4.23 DD132 pKa = 3.74 ACFANCLAPDD142 pKa = 4.02 AQASLTGKK150 pKa = 8.34 LTRR153 pKa = 11.84 VSTFGYY159 pKa = 9.85 SNSARR164 pKa = 11.84 SFASINVVHH173 pKa = 7.13 LPRR176 pKa = 11.84 NYY178 pKa = 10.35 YY179 pKa = 8.24 VTYY182 pKa = 9.03 TQAPPHH188 pKa = 6.24 GSALFMRR195 pKa = 11.84 LVRR198 pKa = 11.84 TRR200 pKa = 11.84 FLVEE204 pKa = 4.01 IQSSAPLGAAGIVVFYY220 pKa = 7.87 FAPADD225 pKa = 3.5 IPEE228 pKa = 4.66 SEE230 pKa = 3.97 GHH232 pKa = 5.96 MGSSVFNLSPCDD244 pKa = 3.54 FDD246 pKa = 3.8 VARR249 pKa = 11.84 MSTAKK254 pKa = 10.22 FVIPYY259 pKa = 9.85 AATTPFVPVDD269 pKa = 3.46 SHH271 pKa = 8.77 DD272 pKa = 3.47 MGRR275 pKa = 11.84 FVCMALTPYY284 pKa = 9.95 RR285 pKa = 11.84 APSGAQSEE293 pKa = 4.75 LTFTIYY299 pKa = 9.43 MAAIEE304 pKa = 4.78 SEE306 pKa = 4.54 LTCPRR311 pKa = 11.84 PRR313 pKa = 11.84 RR314 pKa = 11.84 QMFSTHH320 pKa = 5.45 SQLQEE325 pKa = 3.81 VVITSTPGSLTIGNSSLANLAPTLSLAGEE354 pKa = 5.33 GYY356 pKa = 10.5 RR357 pKa = 11.84 IDD359 pKa = 4.67 EE360 pKa = 4.31 ITPGGQRR367 pKa = 11.84 PVDD370 pKa = 3.72 DD371 pKa = 5.77 FIWLALRR378 pKa = 11.84 PSTRR382 pKa = 11.84 YY383 pKa = 9.92 EE384 pKa = 3.76 GWSFDD389 pKa = 3.45 WHH391 pKa = 6.01 PTDD394 pKa = 4.0 PAEE397 pKa = 4.41 AQLFAWDD404 pKa = 4.72 LDD406 pKa = 4.07 LGMSTSNLGILANSFAYY423 pKa = 10.1 ARR425 pKa = 11.84 GSLQITLVAALSVFNRR441 pKa = 11.84 GRR443 pKa = 11.84 LRR445 pKa = 11.84 LCFEE449 pKa = 3.98 MAGDD453 pKa = 5.0 DD454 pKa = 4.53 RR455 pKa = 11.84 YY456 pKa = 11.59 DD457 pKa = 3.61 HH458 pKa = 5.88 QASMAVNFTVIDD470 pKa = 4.07 FSNSNTGSLVVPYY483 pKa = 8.89 TSPSWFRR490 pKa = 11.84 PTTADD495 pKa = 2.44 HH496 pKa = 7.01 WGRR499 pKa = 11.84 IVVFVNTPLTASAACIDD516 pKa = 4.03 VVSVQVFVSFAPDD529 pKa = 3.03 CKK531 pKa = 10.53 FYY533 pKa = 11.18 VPHH536 pKa = 6.65 SNGMTYY542 pKa = 9.74 QAPPMPDD549 pKa = 2.66 QVSATQCSDD558 pKa = 3.04 PFGPQVTVVDD568 pKa = 5.09 FSQPLVAAEE577 pKa = 4.35 TSPSPHH583 pKa = 7.4 AMFSSAHH590 pKa = 5.22 TNVRR594 pKa = 11.84 HH595 pKa = 5.13 LLGRR599 pKa = 11.84 FWHH602 pKa = 6.1 LTDD605 pKa = 3.81 EE606 pKa = 4.8 FEE608 pKa = 4.41 ATHH611 pKa = 6.11 VLSSHH616 pKa = 7.62 PIPFPANSHH625 pKa = 5.65 AAIARR630 pKa = 11.84 SCAFWNGEE638 pKa = 3.9 PVFVVINHH646 pKa = 5.34 TAGPIEE652 pKa = 4.42 VSHH655 pKa = 6.66 FWEE658 pKa = 4.59 DD659 pKa = 3.25 AYY661 pKa = 11.63 VAVTHH666 pKa = 6.49 ALSSRR671 pKa = 11.84 GSILIPSKK679 pKa = 9.89 GQAVFSVPFYY689 pKa = 10.76 SPTPVRR695 pKa = 11.84 STDD698 pKa = 3.26 ANASLGNLLVRR709 pKa = 11.84 TLEE712 pKa = 4.17 TAVSGRR718 pKa = 11.84 FSVFVAIRR726 pKa = 11.84 NPKK729 pKa = 10.14 LFFPRR734 pKa = 11.84 AIPDD738 pKa = 3.15 WVIPGRR744 pKa = 11.84 SVARR748 pKa = 11.84 TYY750 pKa = 11.04 RR751 pKa = 11.84 SALAALTGAASARR764 pKa = 11.84 ATTSRR769 pKa = 11.84 EE770 pKa = 3.71 SVARR774 pKa = 11.84 IMAAAAALQGPGCFPFGRR792 pKa = 11.84 HH793 pKa = 5.15 SAPVSHH799 pKa = 6.79 SSVASSPRR807 pKa = 11.84 SLLHH811 pKa = 6.01 FLIGRR816 pKa = 11.84 PRR818 pKa = 11.84 PRR820 pKa = 11.84 VPPSPSLLLSGDD832 pKa = 3.77 VEE834 pKa = 4.66 PNPGPVTMVYY844 pKa = 10.77 DD845 pKa = 4.87 PIEE848 pKa = 4.15 CKK850 pKa = 10.67 YY851 pKa = 8.16 YY852 pKa = 11.07 LEE854 pKa = 4.71 VGGHH858 pKa = 6.12 LLGMNPPRR866 pKa = 11.84 GWPAKK871 pKa = 9.19 TKK873 pKa = 10.25 INKK876 pKa = 8.88 KK877 pKa = 10.16 VNTTCRR883 pKa = 11.84 YY884 pKa = 8.02 PKK886 pKa = 8.3 THH888 pKa = 6.68 KK889 pKa = 10.35 CPRR892 pKa = 11.84 WSLFIAQRR900 pKa = 11.84 QPWFISGPIVWDD912 pKa = 4.3 WIMVDD917 pKa = 4.06 YY918 pKa = 10.75 FPLSPEE924 pKa = 5.24 AILCSHH930 pKa = 7.49 GICQIEE936 pKa = 4.08 NDD938 pKa = 4.23 DD939 pKa = 3.65 VHH941 pKa = 8.59 LLMDD945 pKa = 4.64 GVFLPLSRR953 pKa = 11.84 LARR956 pKa = 11.84 MDD958 pKa = 3.78 CAGLISWLFLKK969 pKa = 10.19 MPTWFHH975 pKa = 6.61 CFPFSRR981 pKa = 11.84 PSCPVYY987 pKa = 10.6 LPPEE991 pKa = 3.94 ARR993 pKa = 11.84 AGVTMTAIPQGPFDD1007 pKa = 4.66 TILSFFDD1014 pKa = 4.18 GYY1016 pKa = 11.33 LMKK1019 pKa = 10.54 SAFRR1023 pKa = 11.84 HH1024 pKa = 4.37 YY1025 pKa = 9.34 TPVVLRR1031 pKa = 11.84 CIITLYY1037 pKa = 10.31 SIHH1040 pKa = 6.31 VANDD1044 pKa = 4.32 PIVTLLLGGLATYY1057 pKa = 10.49 DD1058 pKa = 3.16 IMVTKK1063 pKa = 10.12 PPAALVVIIDD1073 pKa = 3.95 AVSCATYY1080 pKa = 10.97 DD1081 pKa = 3.42 SFKK1084 pKa = 11.34 AVMAPVFGDD1093 pKa = 3.5 ITATAWRR1100 pKa = 11.84 RR1101 pKa = 11.84 IKK1103 pKa = 10.71 EE1104 pKa = 3.98 KK1105 pKa = 10.38 FLRR1108 pKa = 11.84 LCARR1112 pKa = 11.84 AQDD1115 pKa = 4.18 FKK1117 pKa = 11.1 KK1118 pKa = 7.96 TTDD1121 pKa = 3.06 TAKK1124 pKa = 10.94 AVVWWISSIAKK1135 pKa = 8.44 MMHH1138 pKa = 5.25 YY1139 pKa = 10.75 VWINWLNPPVQNRR1152 pKa = 11.84 QVQLAVAQLLVDD1164 pKa = 4.37 CNAFLSDD1171 pKa = 4.73 LSLDD1175 pKa = 3.6 PSTLGDD1181 pKa = 3.41 RR1182 pKa = 11.84 PRR1184 pKa = 11.84 RR1185 pKa = 11.84 QRR1187 pKa = 11.84 LLVYY1191 pKa = 9.21 LQRR1194 pKa = 11.84 ALQMPGNPDD1203 pKa = 2.74 NVQRR1207 pKa = 11.84 LLQQSFNKK1215 pKa = 9.95 LVSLSPIPPSPPSTRR1230 pKa = 11.84 VEE1232 pKa = 4.01 PVGVWLSGEE1241 pKa = 4.13 PGTGKK1246 pKa = 10.3 SLLMSALSADD1256 pKa = 3.45 IAKK1259 pKa = 10.01 HH1260 pKa = 4.91 YY1261 pKa = 10.73 NWTVYY1266 pKa = 9.97 NHH1268 pKa = 6.36 PTGSDD1273 pKa = 3.54 YY1274 pKa = 11.32 FDD1276 pKa = 6.01 LYY1278 pKa = 11.05 TNQQVHH1284 pKa = 7.03 CIDD1287 pKa = 5.06 DD1288 pKa = 4.4 LGQGKK1293 pKa = 10.05 AEE1295 pKa = 4.17 EE1296 pKa = 4.24 DD1297 pKa = 3.65 LKK1299 pKa = 11.49 VLCQCISTVPFVVPGAAIEE1318 pKa = 4.35 DD1319 pKa = 3.59 KK1320 pKa = 10.59 KK1321 pKa = 10.39 HH1322 pKa = 5.47 YY1323 pKa = 10.36 YY1324 pKa = 8.6 NGKK1327 pKa = 9.57 VVIATTNRR1335 pKa = 11.84 TDD1337 pKa = 3.35 FRR1339 pKa = 11.84 TYY1341 pKa = 10.75 TLTTPGALEE1350 pKa = 3.72 RR1351 pKa = 11.84 RR1352 pKa = 11.84 FPIRR1356 pKa = 11.84 VRR1358 pKa = 11.84 VLQSKK1363 pKa = 8.84 WPLTKK1368 pKa = 9.53 EE1369 pKa = 4.01 TLRR1372 pKa = 11.84 DD1373 pKa = 3.08 RR1374 pKa = 11.84 TYY1376 pKa = 11.23 FNLVSASEE1384 pKa = 4.0 AAHH1387 pKa = 7.02 PITYY1391 pKa = 10.08 SCLLDD1396 pKa = 4.95 SIIQAYY1402 pKa = 9.79 DD1403 pKa = 2.91 SRR1405 pKa = 11.84 LALLQGPPSPNPFHH1419 pKa = 7.04 LLADD1423 pKa = 3.91 PTFPPPPPEE1432 pKa = 5.78 DD1433 pKa = 3.94 EE1434 pKa = 4.67 PQSCDD1439 pKa = 3.88 DD1440 pKa = 3.97 DD1441 pKa = 3.88 HH1442 pKa = 7.11 LAAIEE1447 pKa = 4.01 ALEE1450 pKa = 4.06 VTPDD1454 pKa = 3.6 SNDD1457 pKa = 3.62 QKK1459 pKa = 10.6 WLEE1462 pKa = 4.19 AVGGSQAADD1471 pKa = 3.55 PDD1473 pKa = 4.2 LPMLSSAPADD1483 pKa = 3.52 PSLLEE1488 pKa = 4.26 RR1489 pKa = 11.84 VTALADD1495 pKa = 3.61 RR1496 pKa = 11.84 LYY1498 pKa = 11.15 GSLRR1502 pKa = 11.84 KK1503 pKa = 8.76 RR1504 pKa = 11.84 TVPEE1508 pKa = 3.62 WALMAFGSLGLFAVLAGIVRR1528 pKa = 11.84 KK1529 pKa = 9.74 IFSFLQPRR1537 pKa = 11.84 PTPPQGPYY1545 pKa = 10.14 NPATAGVRR1553 pKa = 11.84 ISARR1557 pKa = 11.84 EE1558 pKa = 3.74 LARR1561 pKa = 11.84 RR1562 pKa = 11.84 ADD1564 pKa = 4.15 PQSPWQPQFNHH1575 pKa = 6.27 CFKK1578 pKa = 10.74 NCVFIEE1584 pKa = 4.11 ADD1586 pKa = 3.22 GFTWYY1591 pKa = 10.82 AVMFGRR1597 pKa = 11.84 VLVVNKK1603 pKa = 10.14 HH1604 pKa = 6.36 YY1605 pKa = 10.69 LDD1607 pKa = 4.17 CWSGPVVVSTAVSSFSADD1625 pKa = 3.21 LTVPPQFVEE1634 pKa = 3.85 GDD1636 pKa = 3.27 LAYY1639 pKa = 10.54 FHH1641 pKa = 6.96 LPSAPPLKK1649 pKa = 10.24 AAPKK1653 pKa = 9.33 HH1654 pKa = 4.34 YY1655 pKa = 8.93 TVPEE1659 pKa = 4.29 PASGTQAMLLYY1670 pKa = 10.45 AGRR1673 pKa = 11.84 DD1674 pKa = 3.2 GTYY1677 pKa = 11.04 AVTTHH1682 pKa = 5.95 TNSYY1686 pKa = 8.32 YY1687 pKa = 10.58 TFTNGSFFCGIVPSVHH1703 pKa = 6.96 SYY1705 pKa = 9.64 EE1706 pKa = 4.51 VPTQAGMCGGLLVLQVGGNWIPYY1729 pKa = 8.03 AVHH1732 pKa = 6.84 FAGLPHH1738 pKa = 7.13 RR1739 pKa = 11.84 GYY1741 pKa = 11.24 AQGLNLDD1748 pKa = 4.44 WIAAVNAPAHH1758 pKa = 6.39 PLPPLPDD1765 pKa = 4.6 DD1766 pKa = 5.23 ALPAPQGIITKK1777 pKa = 9.87 IEE1779 pKa = 3.82 PTSNFRR1785 pKa = 11.84 LGFCPYY1791 pKa = 9.08 TKK1793 pKa = 10.37 YY1794 pKa = 10.94 SPSPVSLVIRR1804 pKa = 11.84 SEE1806 pKa = 4.22 LEE1808 pKa = 3.77 PAVLSAHH1815 pKa = 6.6 DD1816 pKa = 3.69 NRR1818 pKa = 11.84 LEE1820 pKa = 4.08 VKK1822 pKa = 10.36 RR1823 pKa = 11.84 EE1824 pKa = 3.94 SNAMFLLEE1832 pKa = 4.34 KK1833 pKa = 8.02 TQKK1836 pKa = 10.43 YY1837 pKa = 6.85 DD1838 pKa = 3.38 TNVTVPRR1845 pKa = 11.84 PLLLQTLATEE1855 pKa = 4.51 YY1856 pKa = 9.01 GTHH1859 pKa = 6.38 LRR1861 pKa = 11.84 NLMSTLASPASIEE1874 pKa = 3.97 EE1875 pKa = 4.11 AVFDD1879 pKa = 4.24 TVCPMDD1885 pKa = 3.66 HH1886 pKa = 6.82 RR1887 pKa = 11.84 ASAGPHH1893 pKa = 4.76 YY1894 pKa = 10.21 PGVKK1898 pKa = 9.33 RR1899 pKa = 11.84 SEE1901 pKa = 4.82 LIDD1904 pKa = 3.67 FQRR1907 pKa = 11.84 RR1908 pKa = 11.84 TISDD1912 pKa = 3.63 RR1913 pKa = 11.84 LRR1915 pKa = 11.84 EE1916 pKa = 4.12 DD1917 pKa = 3.51 VVALRR1922 pKa = 11.84 AAFARR1927 pKa = 11.84 GDD1929 pKa = 3.63 NVYY1932 pKa = 10.96 LPFSSFLKK1940 pKa = 10.78 DD1941 pKa = 3.4 EE1942 pKa = 4.78 LRR1944 pKa = 11.84 PKK1946 pKa = 10.3 PKK1948 pKa = 10.0 VRR1950 pKa = 11.84 NGDD1953 pKa = 3.47 TRR1955 pKa = 11.84 VVEE1958 pKa = 4.53 CSSLHH1963 pKa = 4.27 YY1964 pKa = 9.51 TVAFRR1969 pKa = 11.84 MQFLSVLRR1977 pKa = 11.84 MMYY1980 pKa = 10.58 GSDD1983 pKa = 3.65 PNQTGLAPGMNVYY1996 pKa = 10.87 LEE1998 pKa = 4.17 FSTMVSNLYY2007 pKa = 10.05 PNNLCLDD2014 pKa = 4.23 FKK2016 pKa = 11.07 KK2017 pKa = 10.7 YY2018 pKa = 10.66 DD2019 pKa = 3.33 SRR2021 pKa = 11.84 LPSDD2025 pKa = 4.15 VMSVAADD2032 pKa = 3.82 LFASLTEE2039 pKa = 4.31 DD2040 pKa = 3.37 PVVSRR2045 pKa = 11.84 RR2046 pKa = 11.84 YY2047 pKa = 9.21 FDD2049 pKa = 5.32 PIIDD2053 pKa = 4.65 SIHH2056 pKa = 5.91 EE2057 pKa = 3.94 VGPYY2061 pKa = 9.21 RR2062 pKa = 11.84 VEE2064 pKa = 3.85 VHH2066 pKa = 6.29 GGMPSGCAVTTLLNSVCNVLMCSYY2090 pKa = 11.07 AVLLQDD2096 pKa = 4.32 PDD2098 pKa = 3.04 MDD2100 pKa = 4.18 FQVVAYY2106 pKa = 9.77 GDD2108 pKa = 4.01 DD2109 pKa = 4.37 NIVSTAEE2116 pKa = 4.04 PLDD2119 pKa = 3.78 VPAFRR2124 pKa = 11.84 SVLASDD2130 pKa = 4.54 FGMVTTSADD2139 pKa = 3.35 KK2140 pKa = 11.18 SEE2142 pKa = 4.36 EE2143 pKa = 4.38 CYY2145 pKa = 10.0 QVPPEE2150 pKa = 4.01 QVDD2153 pKa = 3.82 FLKK2156 pKa = 10.74 RR2157 pKa = 11.84 RR2158 pKa = 11.84 LRR2160 pKa = 11.84 WTSDD2164 pKa = 3.15 FPVPVPVLPLDD2175 pKa = 3.82 SMLSRR2180 pKa = 11.84 LCWCKK2185 pKa = 10.5 GPHH2188 pKa = 5.85 EE2189 pKa = 4.88 FRR2191 pKa = 11.84 DD2192 pKa = 3.66 QLISFSYY2199 pKa = 10.33 EE2200 pKa = 3.37 LGFYY2204 pKa = 9.41 GQEE2207 pKa = 3.74 VYY2209 pKa = 10.82 HH2210 pKa = 6.38 RR2211 pKa = 11.84 VFSALLPHH2219 pKa = 6.61 ATLPPWSQTLQSCRR2233 pKa = 11.84 YY2234 pKa = 9.2 LLGIEE2239 pKa = 4.74 DD2240 pKa = 4.51 ALHH2243 pKa = 6.55 PAGKK2247 pKa = 8.26 TT2248 pKa = 3.32
Molecular weight: 245.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.926
IPC2_protein 5.931
IPC_protein 6.02
Toseland 6.364
ProMoST 6.427
Dawson 6.351
Bjellqvist 6.351
Wikipedia 6.376
Rodwell 6.351
Grimsley 6.504
Solomon 6.351
Lehninger 6.351
Nozaki 6.649
DTASelect 6.825
Thurlkill 6.854
EMBOSS 6.81
Sillero 6.751
Patrickios 3.808
IPC_peptide 6.364
IPC2_peptide 6.722
IPC2.peptide.svr19 6.64
Protein with the highest isoelectric point:
>tr|S4VD62|S4VD62_9PICO Capsid protein VP0 OS=Kunsagivirus A OX=1755590 PE=4 SV=1
MM1 pKa = 8.08 DD2 pKa = 6.06 PLSLTAGLVGDD13 pKa = 4.12 VVGGVAGAITGGGDD27 pKa = 3.5 PVGSLLSSGLSLLGLGSSSSTSSPTPSRR55 pKa = 11.84 DD56 pKa = 3.02 GGGPSSIGPQLSAPDD71 pKa = 4.33 LPQPPPSPGLPLSAGGDD88 pKa = 3.88 PQPHH92 pKa = 6.2 GVTSSEE98 pKa = 4.32 PPTPAPTHH106 pKa = 5.07 TQAGSSQSTATPFGGASSGHH126 pKa = 5.7 TPTFEE131 pKa = 4.23 DD132 pKa = 3.74 ACFANCLAPDD142 pKa = 4.02 AQASLTGKK150 pKa = 8.34 LTRR153 pKa = 11.84 VSTFGYY159 pKa = 9.85 SNSARR164 pKa = 11.84 SFASINVVHH173 pKa = 7.13 LPRR176 pKa = 11.84 NYY178 pKa = 10.35 YY179 pKa = 8.24 VTYY182 pKa = 9.03 TQAPPHH188 pKa = 6.24 GSALFMRR195 pKa = 11.84 LVRR198 pKa = 11.84 TRR200 pKa = 11.84 FLVEE204 pKa = 4.01 IQSSAPLGAAGIVVFYY220 pKa = 7.87 FAPADD225 pKa = 3.5 IPEE228 pKa = 4.66 SEE230 pKa = 3.97 GHH232 pKa = 5.96 MGSSVFNLSPCDD244 pKa = 3.54 FDD246 pKa = 3.8 VARR249 pKa = 11.84 MSTAKK254 pKa = 10.22 FVIPYY259 pKa = 9.85 AATTPFVPVDD269 pKa = 3.46 SHH271 pKa = 8.77 DD272 pKa = 3.47 MGRR275 pKa = 11.84 FVCMALTPYY284 pKa = 9.95 RR285 pKa = 11.84 APSGAQSEE293 pKa = 4.75 LTFTIYY299 pKa = 9.43 MAAIEE304 pKa = 4.78 SEE306 pKa = 4.54 LTCPRR311 pKa = 11.84 PRR313 pKa = 11.84 RR314 pKa = 11.84 QMFSTHH320 pKa = 5.45 SQLQEE325 pKa = 3.81 VVITSTPGSLTIGNSSLANLAPTLSLAGEE354 pKa = 5.33 GYY356 pKa = 10.5 RR357 pKa = 11.84 IDD359 pKa = 4.67 EE360 pKa = 4.31 ITPGGQRR367 pKa = 11.84 PVDD370 pKa = 3.72 DD371 pKa = 5.77 FIWLALRR378 pKa = 11.84 PSTRR382 pKa = 11.84 YY383 pKa = 9.92 EE384 pKa = 3.76 GWSFDD389 pKa = 3.45 WHH391 pKa = 6.01 PTDD394 pKa = 4.0 PAEE397 pKa = 4.41 AQLFAWDD404 pKa = 4.72 LDD406 pKa = 4.07 LGMSTSNLGILANSFAYY423 pKa = 10.1 ARR425 pKa = 11.84 GSLQITLVAALSVFNRR441 pKa = 11.84 GRR443 pKa = 11.84 LRR445 pKa = 11.84 LCFEE449 pKa = 3.98 MAGDD453 pKa = 5.0 DD454 pKa = 4.53 RR455 pKa = 11.84 YY456 pKa = 11.59 DD457 pKa = 3.61 HH458 pKa = 5.88 QASMAVNFTVIDD470 pKa = 4.07 FSNSNTGSLVVPYY483 pKa = 8.89 TSPSWFRR490 pKa = 11.84 PTTADD495 pKa = 2.44 HH496 pKa = 7.01 WGRR499 pKa = 11.84 IVVFVNTPLTASAACIDD516 pKa = 4.03 VVSVQVFVSFAPDD529 pKa = 3.03 CKK531 pKa = 10.53 FYY533 pKa = 11.18 VPHH536 pKa = 6.65 SNGMTYY542 pKa = 9.74 QAPPMPDD549 pKa = 2.66 QVSATQCSDD558 pKa = 3.04 PFGPQVTVVDD568 pKa = 5.09 FSQPLVAAEE577 pKa = 4.35 TSPSPHH583 pKa = 7.4 AMFSSAHH590 pKa = 5.22 TNVRR594 pKa = 11.84 HH595 pKa = 5.13 LLGRR599 pKa = 11.84 FWHH602 pKa = 6.1 LTDD605 pKa = 3.81 EE606 pKa = 4.8 FEE608 pKa = 4.41 ATHH611 pKa = 6.11 VLSSHH616 pKa = 7.62 PIPFPANSHH625 pKa = 5.65 AAIARR630 pKa = 11.84 SCAFWNGEE638 pKa = 3.9 PVFVVINHH646 pKa = 5.34 TAGPIEE652 pKa = 4.42 VSHH655 pKa = 6.66 FWEE658 pKa = 4.59 DD659 pKa = 3.25 AYY661 pKa = 11.63 VAVTHH666 pKa = 6.49 ALSSRR671 pKa = 11.84 GSILIPSKK679 pKa = 9.89 GQAVFSVPFYY689 pKa = 10.76 SPTPVRR695 pKa = 11.84 STDD698 pKa = 3.26 ANASLGNLLVRR709 pKa = 11.84 TLEE712 pKa = 4.17 TAVSGRR718 pKa = 11.84 FSVFVAIRR726 pKa = 11.84 NPKK729 pKa = 10.14 LFFPRR734 pKa = 11.84 AIPDD738 pKa = 3.15 WVIPGRR744 pKa = 11.84 SVARR748 pKa = 11.84 TYY750 pKa = 11.04 RR751 pKa = 11.84 SALAALTGAASARR764 pKa = 11.84 ATTSRR769 pKa = 11.84 EE770 pKa = 3.71 SVARR774 pKa = 11.84 IMAAAAALQGPGCFPFGRR792 pKa = 11.84 HH793 pKa = 5.15 SAPVSHH799 pKa = 6.79 SSVASSPRR807 pKa = 11.84 SLLHH811 pKa = 6.01 FLIGRR816 pKa = 11.84 PRR818 pKa = 11.84 PRR820 pKa = 11.84 VPPSPSLLLSGDD832 pKa = 3.77 VEE834 pKa = 4.66 PNPGPVTMVYY844 pKa = 10.77 DD845 pKa = 4.87 PIEE848 pKa = 4.15 CKK850 pKa = 10.67 YY851 pKa = 8.16 YY852 pKa = 11.07 LEE854 pKa = 4.71 VGGHH858 pKa = 6.12 LLGMNPPRR866 pKa = 11.84 GWPAKK871 pKa = 9.19 TKK873 pKa = 10.25 INKK876 pKa = 8.88 KK877 pKa = 10.16 VNTTCRR883 pKa = 11.84 YY884 pKa = 8.02 PKK886 pKa = 8.3 THH888 pKa = 6.68 KK889 pKa = 10.35 CPRR892 pKa = 11.84 WSLFIAQRR900 pKa = 11.84 QPWFISGPIVWDD912 pKa = 4.3 WIMVDD917 pKa = 4.06 YY918 pKa = 10.75 FPLSPEE924 pKa = 5.24 AILCSHH930 pKa = 7.49 GICQIEE936 pKa = 4.08 NDD938 pKa = 4.23 DD939 pKa = 3.65 VHH941 pKa = 8.59 LLMDD945 pKa = 4.64 GVFLPLSRR953 pKa = 11.84 LARR956 pKa = 11.84 MDD958 pKa = 3.78 CAGLISWLFLKK969 pKa = 10.19 MPTWFHH975 pKa = 6.61 CFPFSRR981 pKa = 11.84 PSCPVYY987 pKa = 10.6 LPPEE991 pKa = 3.94 ARR993 pKa = 11.84 AGVTMTAIPQGPFDD1007 pKa = 4.66 TILSFFDD1014 pKa = 4.18 GYY1016 pKa = 11.33 LMKK1019 pKa = 10.54 SAFRR1023 pKa = 11.84 HH1024 pKa = 4.37 YY1025 pKa = 9.34 TPVVLRR1031 pKa = 11.84 CIITLYY1037 pKa = 10.31 SIHH1040 pKa = 6.31 VANDD1044 pKa = 4.32 PIVTLLLGGLATYY1057 pKa = 10.49 DD1058 pKa = 3.16 IMVTKK1063 pKa = 10.12 PPAALVVIIDD1073 pKa = 3.95 AVSCATYY1080 pKa = 10.97 DD1081 pKa = 3.42 SFKK1084 pKa = 11.34 AVMAPVFGDD1093 pKa = 3.5 ITATAWRR1100 pKa = 11.84 RR1101 pKa = 11.84 IKK1103 pKa = 10.71 EE1104 pKa = 3.98 KK1105 pKa = 10.38 FLRR1108 pKa = 11.84 LCARR1112 pKa = 11.84 AQDD1115 pKa = 4.18 FKK1117 pKa = 11.1 KK1118 pKa = 7.96 TTDD1121 pKa = 3.06 TAKK1124 pKa = 10.94 AVVWWISSIAKK1135 pKa = 8.44 MMHH1138 pKa = 5.25 YY1139 pKa = 10.75 VWINWLNPPVQNRR1152 pKa = 11.84 QVQLAVAQLLVDD1164 pKa = 4.37 CNAFLSDD1171 pKa = 4.73 LSLDD1175 pKa = 3.6 PSTLGDD1181 pKa = 3.41 RR1182 pKa = 11.84 PRR1184 pKa = 11.84 RR1185 pKa = 11.84 QRR1187 pKa = 11.84 LLVYY1191 pKa = 9.21 LQRR1194 pKa = 11.84 ALQMPGNPDD1203 pKa = 2.74 NVQRR1207 pKa = 11.84 LLQQSFNKK1215 pKa = 9.95 LVSLSPIPPSPPSTRR1230 pKa = 11.84 VEE1232 pKa = 4.01 PVGVWLSGEE1241 pKa = 4.13 PGTGKK1246 pKa = 10.3 SLLMSALSADD1256 pKa = 3.45 IAKK1259 pKa = 10.01 HH1260 pKa = 4.91 YY1261 pKa = 10.73 NWTVYY1266 pKa = 9.97 NHH1268 pKa = 6.36 PTGSDD1273 pKa = 3.54 YY1274 pKa = 11.32 FDD1276 pKa = 6.01 LYY1278 pKa = 11.05 TNQQVHH1284 pKa = 7.03 CIDD1287 pKa = 5.06 DD1288 pKa = 4.4 LGQGKK1293 pKa = 10.05 AEE1295 pKa = 4.17 EE1296 pKa = 4.24 DD1297 pKa = 3.65 LKK1299 pKa = 11.49 VLCQCISTVPFVVPGAAIEE1318 pKa = 4.35 DD1319 pKa = 3.59 KK1320 pKa = 10.59 KK1321 pKa = 10.39 HH1322 pKa = 5.47 YY1323 pKa = 10.36 YY1324 pKa = 8.6 NGKK1327 pKa = 9.57 VVIATTNRR1335 pKa = 11.84 TDD1337 pKa = 3.35 FRR1339 pKa = 11.84 TYY1341 pKa = 10.75 TLTTPGALEE1350 pKa = 3.72 RR1351 pKa = 11.84 RR1352 pKa = 11.84 FPIRR1356 pKa = 11.84 VRR1358 pKa = 11.84 VLQSKK1363 pKa = 8.84 WPLTKK1368 pKa = 9.53 EE1369 pKa = 4.01 TLRR1372 pKa = 11.84 DD1373 pKa = 3.08 RR1374 pKa = 11.84 TYY1376 pKa = 11.23 FNLVSASEE1384 pKa = 4.0 AAHH1387 pKa = 7.02 PITYY1391 pKa = 10.08 SCLLDD1396 pKa = 4.95 SIIQAYY1402 pKa = 9.79 DD1403 pKa = 2.91 SRR1405 pKa = 11.84 LALLQGPPSPNPFHH1419 pKa = 7.04 LLADD1423 pKa = 3.91 PTFPPPPPEE1432 pKa = 5.78 DD1433 pKa = 3.94 EE1434 pKa = 4.67 PQSCDD1439 pKa = 3.88 DD1440 pKa = 3.97 DD1441 pKa = 3.88 HH1442 pKa = 7.11 LAAIEE1447 pKa = 4.01 ALEE1450 pKa = 4.06 VTPDD1454 pKa = 3.6 SNDD1457 pKa = 3.62 QKK1459 pKa = 10.6 WLEE1462 pKa = 4.19 AVGGSQAADD1471 pKa = 3.55 PDD1473 pKa = 4.2 LPMLSSAPADD1483 pKa = 3.52 PSLLEE1488 pKa = 4.26 RR1489 pKa = 11.84 VTALADD1495 pKa = 3.61 RR1496 pKa = 11.84 LYY1498 pKa = 11.15 GSLRR1502 pKa = 11.84 KK1503 pKa = 8.76 RR1504 pKa = 11.84 TVPEE1508 pKa = 3.62 WALMAFGSLGLFAVLAGIVRR1528 pKa = 11.84 KK1529 pKa = 9.74 IFSFLQPRR1537 pKa = 11.84 PTPPQGPYY1545 pKa = 10.14 NPATAGVRR1553 pKa = 11.84 ISARR1557 pKa = 11.84 EE1558 pKa = 3.74 LARR1561 pKa = 11.84 RR1562 pKa = 11.84 ADD1564 pKa = 4.15 PQSPWQPQFNHH1575 pKa = 6.27 CFKK1578 pKa = 10.74 NCVFIEE1584 pKa = 4.11 ADD1586 pKa = 3.22 GFTWYY1591 pKa = 10.82 AVMFGRR1597 pKa = 11.84 VLVVNKK1603 pKa = 10.14 HH1604 pKa = 6.36 YY1605 pKa = 10.69 LDD1607 pKa = 4.17 CWSGPVVVSTAVSSFSADD1625 pKa = 3.21 LTVPPQFVEE1634 pKa = 3.85 GDD1636 pKa = 3.27 LAYY1639 pKa = 10.54 FHH1641 pKa = 6.96 LPSAPPLKK1649 pKa = 10.24 AAPKK1653 pKa = 9.33 HH1654 pKa = 4.34 YY1655 pKa = 8.93 TVPEE1659 pKa = 4.29 PASGTQAMLLYY1670 pKa = 10.45 AGRR1673 pKa = 11.84 DD1674 pKa = 3.2 GTYY1677 pKa = 11.04 AVTTHH1682 pKa = 5.95 TNSYY1686 pKa = 8.32 YY1687 pKa = 10.58 TFTNGSFFCGIVPSVHH1703 pKa = 6.96 SYY1705 pKa = 9.64 EE1706 pKa = 4.51 VPTQAGMCGGLLVLQVGGNWIPYY1729 pKa = 8.03 AVHH1732 pKa = 6.84 FAGLPHH1738 pKa = 7.13 RR1739 pKa = 11.84 GYY1741 pKa = 11.24 AQGLNLDD1748 pKa = 4.44 WIAAVNAPAHH1758 pKa = 6.39 PLPPLPDD1765 pKa = 4.6 DD1766 pKa = 5.23 ALPAPQGIITKK1777 pKa = 9.87 IEE1779 pKa = 3.82 PTSNFRR1785 pKa = 11.84 LGFCPYY1791 pKa = 9.08 TKK1793 pKa = 10.37 YY1794 pKa = 10.94 SPSPVSLVIRR1804 pKa = 11.84 SEE1806 pKa = 4.22 LEE1808 pKa = 3.77 PAVLSAHH1815 pKa = 6.6 DD1816 pKa = 3.69 NRR1818 pKa = 11.84 LEE1820 pKa = 4.08 VKK1822 pKa = 10.36 RR1823 pKa = 11.84 EE1824 pKa = 3.94 SNAMFLLEE1832 pKa = 4.34 KK1833 pKa = 8.02 TQKK1836 pKa = 10.43 YY1837 pKa = 6.85 DD1838 pKa = 3.38 TNVTVPRR1845 pKa = 11.84 PLLLQTLATEE1855 pKa = 4.51 YY1856 pKa = 9.01 GTHH1859 pKa = 6.38 LRR1861 pKa = 11.84 NLMSTLASPASIEE1874 pKa = 3.97 EE1875 pKa = 4.11 AVFDD1879 pKa = 4.24 TVCPMDD1885 pKa = 3.66 HH1886 pKa = 6.82 RR1887 pKa = 11.84 ASAGPHH1893 pKa = 4.76 YY1894 pKa = 10.21 PGVKK1898 pKa = 9.33 RR1899 pKa = 11.84 SEE1901 pKa = 4.82 LIDD1904 pKa = 3.67 FQRR1907 pKa = 11.84 RR1908 pKa = 11.84 TISDD1912 pKa = 3.63 RR1913 pKa = 11.84 LRR1915 pKa = 11.84 EE1916 pKa = 4.12 DD1917 pKa = 3.51 VVALRR1922 pKa = 11.84 AAFARR1927 pKa = 11.84 GDD1929 pKa = 3.63 NVYY1932 pKa = 10.96 LPFSSFLKK1940 pKa = 10.78 DD1941 pKa = 3.4 EE1942 pKa = 4.78 LRR1944 pKa = 11.84 PKK1946 pKa = 10.3 PKK1948 pKa = 10.0 VRR1950 pKa = 11.84 NGDD1953 pKa = 3.47 TRR1955 pKa = 11.84 VVEE1958 pKa = 4.53 CSSLHH1963 pKa = 4.27 YY1964 pKa = 9.51 TVAFRR1969 pKa = 11.84 MQFLSVLRR1977 pKa = 11.84 MMYY1980 pKa = 10.58 GSDD1983 pKa = 3.65 PNQTGLAPGMNVYY1996 pKa = 10.87 LEE1998 pKa = 4.17 FSTMVSNLYY2007 pKa = 10.05 PNNLCLDD2014 pKa = 4.23 FKK2016 pKa = 11.07 KK2017 pKa = 10.7 YY2018 pKa = 10.66 DD2019 pKa = 3.33 SRR2021 pKa = 11.84 LPSDD2025 pKa = 4.15 VMSVAADD2032 pKa = 3.82 LFASLTEE2039 pKa = 4.31 DD2040 pKa = 3.37 PVVSRR2045 pKa = 11.84 RR2046 pKa = 11.84 YY2047 pKa = 9.21 FDD2049 pKa = 5.32 PIIDD2053 pKa = 4.65 SIHH2056 pKa = 5.91 EE2057 pKa = 3.94 VGPYY2061 pKa = 9.21 RR2062 pKa = 11.84 VEE2064 pKa = 3.85 VHH2066 pKa = 6.29 GGMPSGCAVTTLLNSVCNVLMCSYY2090 pKa = 11.07 AVLLQDD2096 pKa = 4.32 PDD2098 pKa = 3.04 MDD2100 pKa = 4.18 FQVVAYY2106 pKa = 9.77 GDD2108 pKa = 4.01 DD2109 pKa = 4.37 NIVSTAEE2116 pKa = 4.04 PLDD2119 pKa = 3.78 VPAFRR2124 pKa = 11.84 SVLASDD2130 pKa = 4.54 FGMVTTSADD2139 pKa = 3.35 KK2140 pKa = 11.18 SEE2142 pKa = 4.36 EE2143 pKa = 4.38 CYY2145 pKa = 10.0 QVPPEE2150 pKa = 4.01 QVDD2153 pKa = 3.82 FLKK2156 pKa = 10.74 RR2157 pKa = 11.84 RR2158 pKa = 11.84 LRR2160 pKa = 11.84 WTSDD2164 pKa = 3.15 FPVPVPVLPLDD2175 pKa = 3.82 SMLSRR2180 pKa = 11.84 LCWCKK2185 pKa = 10.5 GPHH2188 pKa = 5.85 EE2189 pKa = 4.88 FRR2191 pKa = 11.84 DD2192 pKa = 3.66 QLISFSYY2199 pKa = 10.33 EE2200 pKa = 3.37 LGFYY2204 pKa = 9.41 GQEE2207 pKa = 3.74 VYY2209 pKa = 10.82 HH2210 pKa = 6.38 RR2211 pKa = 11.84 VFSALLPHH2219 pKa = 6.61 ATLPPWSQTLQSCRR2233 pKa = 11.84 YY2234 pKa = 9.2 LLGIEE2239 pKa = 4.74 DD2240 pKa = 4.51 ALHH2243 pKa = 6.55 PAGKK2247 pKa = 8.26 TT2248 pKa = 3.32
Molecular weight: 245.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.926
IPC2_protein 5.931
IPC_protein 6.02
Toseland 6.364
ProMoST 6.427
Dawson 6.351
Bjellqvist 6.351
Wikipedia 6.376
Rodwell 6.351
Grimsley 6.504
Solomon 6.351
Lehninger 6.351
Nozaki 6.649
DTASelect 6.825
Thurlkill 6.854
EMBOSS 6.81
Sillero 6.751
Patrickios 3.808
IPC_peptide 6.364
IPC2_peptide 6.722
IPC2.peptide.svr19 6.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
2248
2248
2248
2248.0
245.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.986 ± 0.0
1.957 ± 0.0
5.249 ± 0.0
3.292 ± 0.0
4.938 ± 0.0
6.094 ± 0.0
2.669 ± 0.0
3.781 ± 0.0
2.402 ± 0.0
9.564 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.135 ± 0.0
2.98 ± 0.0
9.253 ± 0.0
3.247 ± 0.0
5.294 ± 0.0
9.075 ± 0.0
6.272 ± 0.0
7.963 ± 0.0
1.557 ± 0.0
3.292 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here