Paramesorhizobium deserti
Average proteome isoelectric point is 6.48
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4946 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A135HY13|A0A135HY13_9RHIZ LysM domain-containing protein OS=Paramesorhizobium deserti OX=1494590 GN=ATN84_24220 PE=4 SV=1
EEE2 pKa = 4.55 EEE4 pKa = 4.14 GTLRR8 pKa = 11.84 TYYY11 pKa = 10.87 EEE13 pKa = 4.69 RR14 pKa = 11.84 FQWDDD19 pKa = 3.58 NDDD22 pKa = 2.84 DDD24 pKa = 3.94 AYYY27 pKa = 10.45 EEE29 pKa = 4.54 DDD31 pKa = 3.35 DD32 pKa = 3.72 TLNFAYYY39 pKa = 9.67 EEE41 pKa = 4.18 GGLRR45 pKa = 11.84 IGKKK49 pKa = 9.54 DD50 pKa = 3.21 EE51 pKa = 4.22 AFRR54 pKa = 11.84 TFLGYYY60 pKa = 10.54 GNVINDDD67 pKa = 3.95 DD68 pKa = 3.74 IEEE71 pKa = 4.17 LNYYY75 pKa = 8.18 DD76 pKa = 3.57 TLISYYY82 pKa = 7.51 FTGGNGISAILSAEEE97 pKa = 4.0 GEEE100 pKa = 4.55 DDD102 pKa = 3.92 YY103 pKa = 10.83 INDDD107 pKa = 4.64 YY108 pKa = 10.61 PHHH111 pKa = 5.85 VGGLKKK117 pKa = 9.02 AQGWGSIGAVVGYYY131 pKa = 10.49 DD132 pKa = 3.61 VIEEE136 pKa = 4.21 EE137 pKa = 4.24 YY138 pKa = 10.52 IKKK141 pKa = 10.67 RR142 pKa = 11.84 VDDD145 pKa = 4.42 NVSDDD150 pKa = 4.16 FSVFVMGAWQSDDD163 pKa = 3.66 EEE165 pKa = 4.41 INFRR169 pKa = 11.84 EEE171 pKa = 3.95 FYYY174 pKa = 11.22 SWGGDDD180 pKa = 2.66 AAWVGASYYY189 pKa = 11.55 KK190 pKa = 9.98 TDDD193 pKa = 3.4 KK194 pKa = 10.81 TFNAQVSYYY203 pKa = 11.73 DD204 pKa = 4.61 DD205 pKa = 4.29 DDD207 pKa = 4.04 LAVAANVAYYY217 pKa = 9.79 EE218 pKa = 4.07 VPGFTITPEEE228 pKa = 3.56 LYYY231 pKa = 11.18 YY232 pKa = 10.5 KK233 pKa = 10.63 DD234 pKa = 4.6 VDDD237 pKa = 4.67 DD238 pKa = 4.26 SPAGVITQSDDD249 pKa = 4.2 DD250 pKa = 2.64 WGGMIRR256 pKa = 11.84 FQRR259 pKa = 11.84 SF
Molecular weight: 28.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.617
ProMoST 4.024
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.77
Nozaki 3.935
DTASelect 4.177
Thurlkill 3.668
EMBOSS 3.77
Sillero 3.948
Patrickios 1.189
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A135I065|A0A135I065_9RHIZ Sugar ABC transporter permease OS=Paramesorhizobium deserti OX=1494590 GN=ATN84_03530 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATPGGRR28 pKa = 11.84 KK29 pKa = 9.26 VIAARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.03 RR41 pKa = 11.84 LSAA44 pKa = 4.03
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4946
0
4946
1548363
29
3682
313.1
34.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.825 ± 0.041
0.779 ± 0.012
5.584 ± 0.031
5.945 ± 0.033
3.919 ± 0.023
8.496 ± 0.043
2.038 ± 0.018
5.871 ± 0.028
3.591 ± 0.031
10.019 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.017
2.821 ± 0.023
5.02 ± 0.027
3.018 ± 0.019
6.842 ± 0.038
5.487 ± 0.028
5.269 ± 0.034
7.254 ± 0.029
1.321 ± 0.017
2.345 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here