Mapuera orthorubulavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Rubulavirinae; Orthorubulavirus

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A3R042|A3R042_9MONO Isoform of A3R040 W protein OS=Mapuera orthorubulavirus OX=2560582 GN=P/V PE=4 SV=1
MM1 pKa = 8.2DD2 pKa = 4.36LTFSPSEE9 pKa = 3.74IDD11 pKa = 4.2DD12 pKa = 4.09LFGTGLDD19 pKa = 3.74TIQFITDD26 pKa = 3.32QKK28 pKa = 11.21SKK30 pKa = 11.15QNDD33 pKa = 3.12AHH35 pKa = 6.85GSAKK39 pKa = 10.42DD40 pKa = 4.46SPPQTQNGPDD50 pKa = 3.99SGPSDD55 pKa = 3.5PTQVQGAKK63 pKa = 9.38PKK65 pKa = 10.41SHH67 pKa = 7.21GIYY70 pKa = 10.01PPIPTAPPVPTARR83 pKa = 11.84HH84 pKa = 5.39PGSRR88 pKa = 11.84VDD90 pKa = 5.59DD91 pKa = 4.12PVLYY95 pKa = 10.25DD96 pKa = 3.05YY97 pKa = 10.65PRR99 pKa = 11.84RR100 pKa = 11.84GKK102 pKa = 8.82VTTHH106 pKa = 5.88EE107 pKa = 4.46PKK109 pKa = 10.46EE110 pKa = 4.07SSQADD115 pKa = 3.93GYY117 pKa = 11.51EE118 pKa = 3.94YY119 pKa = 10.85DD120 pKa = 4.98SYY122 pKa = 11.54LAQNAKK128 pKa = 9.76TNILKK133 pKa = 10.35RR134 pKa = 11.84WTDD137 pKa = 3.08VSGDD141 pKa = 3.69VEE143 pKa = 5.12PIPMNPEE150 pKa = 3.67VFKK153 pKa = 11.13RR154 pKa = 11.84GADD157 pKa = 3.42LTKK160 pKa = 9.41PTNPNPGHH168 pKa = 5.86RR169 pKa = 11.84RR170 pKa = 11.84EE171 pKa = 4.06WSIGWVGSTVKK182 pKa = 10.54VLEE185 pKa = 4.37WCNPTCSPITATSRR199 pKa = 11.84YY200 pKa = 8.01YY201 pKa = 10.71EE202 pKa = 4.69CVCGICPKK210 pKa = 10.03ICPRR214 pKa = 11.84CVGDD218 pKa = 3.58YY219 pKa = 11.08GHH221 pKa = 6.85VEE223 pKa = 3.69TAGRR227 pKa = 11.84KK228 pKa = 8.34DD229 pKa = 3.02WLSRR233 pKa = 11.84GEE235 pKa = 4.57DD236 pKa = 3.73GSDD239 pKa = 2.95SSSRR243 pKa = 11.84GDD245 pKa = 3.6CEE247 pKa = 4.4SDD249 pKa = 3.29QEE251 pKa = 4.24

Molecular weight: 27.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A3R043|A3R043_9MONO Isoform of A3R040 C protein OS=Mapuera orthorubulavirus OX=2560582 GN=P/V PE=4 SV=1
MM1 pKa = 7.42TYY3 pKa = 10.34SVQGWTPSSSLQIKK17 pKa = 8.45SQSRR21 pKa = 11.84MMLMALQRR29 pKa = 11.84TAPRR33 pKa = 11.84KK34 pKa = 9.98LRR36 pKa = 11.84MDD38 pKa = 4.12PTPAQAIQHH47 pKa = 5.66RR48 pKa = 11.84CKK50 pKa = 10.7VQNQRR55 pKa = 11.84ATEE58 pKa = 4.13FTHH61 pKa = 7.49LFLLHH66 pKa = 6.82LLCPLQGTLARR77 pKa = 11.84EE78 pKa = 3.93LTIQFSMTTLDD89 pKa = 3.11VGKK92 pKa = 10.56

Molecular weight: 10.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

3

9

5215

92

2253

579.4

64.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.52 ± 0.806

1.86 ± 0.266

5.388 ± 0.564

4.909 ± 0.425

3.394 ± 0.438

5.005 ± 0.63

2.109 ± 0.319

7.344 ± 0.59

4.506 ± 0.457

10.048 ± 1.263

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.42

3.912 ± 0.432

5.925 ± 0.936

5.139 ± 0.637

5.043 ± 0.302

9.128 ± 0.477

6.635 ± 0.452

5.887 ± 0.508

1.285 ± 0.237

3.528 ± 0.325
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski