Nitrosospira briensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosospira

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2913 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4YPQ9|A0A1I4YPQ9_9PROT Uncharacterized protein OS=Nitrosospira briensis OX=35799 GN=SAMN05216386_0816 PE=4 SV=1
MM1 pKa = 7.47VSVPWTLPGASFDD14 pKa = 4.14ATPGTPFTLGCLTFHH29 pKa = 6.8NGEE32 pKa = 4.24ILSGTGADD40 pKa = 3.07SVNFRR45 pKa = 11.84LAINFDD51 pKa = 3.71NVPEE55 pKa = 4.24KK56 pKa = 11.08NLVLEE61 pKa = 4.79TPFHH65 pKa = 6.11LTNTPNVDD73 pKa = 3.88DD74 pKa = 4.85PVAAADD80 pKa = 4.05FVSIGNFNFTFNVFEE95 pKa = 4.74GNTASVDD102 pKa = 3.29ILATLSTGLTATMAQVCLLNGCC124 pKa = 4.45

Molecular weight: 13.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4XFE3|A0A1I4XFE3_9PROT ParB-like nuclease domain-containing protein OS=Nitrosospira briensis OX=35799 GN=SAMN05216386_0099 PE=4 SV=1
MM1 pKa = 7.56LSLYY5 pKa = 9.67MEE7 pKa = 4.41NAMKK11 pKa = 10.6NKK13 pKa = 9.75RR14 pKa = 11.84CAACDD19 pKa = 3.08RR20 pKa = 11.84LFRR23 pKa = 11.84PRR25 pKa = 11.84PQTPNQSFCSNTEE38 pKa = 3.78CQRR41 pKa = 11.84EE42 pKa = 4.23RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84QWQRR49 pKa = 11.84LKK51 pKa = 11.09LQADD55 pKa = 3.89PDD57 pKa = 4.12YY58 pKa = 11.33QDD60 pKa = 3.15NQARR64 pKa = 11.84AQRR67 pKa = 11.84AWNKK71 pKa = 10.11RR72 pKa = 11.84NPDD75 pKa = 2.79YY76 pKa = 10.37WRR78 pKa = 11.84EE79 pKa = 3.71YY80 pKa = 9.04RR81 pKa = 11.84QSHH84 pKa = 4.83RR85 pKa = 11.84QYY87 pKa = 10.87VEE89 pKa = 3.73RR90 pKa = 11.84NRR92 pKa = 11.84AMQQQRR98 pKa = 11.84NAKK101 pKa = 9.68ARR103 pKa = 11.84VGQVAKK109 pKa = 10.02MDD111 pKa = 3.63VSDD114 pKa = 4.26PLARR118 pKa = 11.84PPSGFYY124 pKa = 10.36RR125 pKa = 11.84LSLIPDD131 pKa = 3.27NGIAKK136 pKa = 9.46VDD138 pKa = 3.28VWTVEE143 pKa = 4.06IRR145 pKa = 11.84VHH147 pKa = 5.78ACKK150 pKa = 10.35CMRR153 pKa = 11.84SANIAKK159 pKa = 10.15RR160 pKa = 3.49

Molecular weight: 19.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2913

0

2913

933013

29

3992

320.3

35.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.04 ± 0.05

0.981 ± 0.017

5.251 ± 0.034

6.164 ± 0.042

3.907 ± 0.034

7.78 ± 0.046

2.363 ± 0.023

5.722 ± 0.036

4.209 ± 0.035

10.412 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.493 ± 0.022

3.437 ± 0.029

4.933 ± 0.033

3.719 ± 0.028

6.459 ± 0.038

6.04 ± 0.037

5.119 ± 0.031

6.922 ± 0.041

1.327 ± 0.02

2.722 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski