Beihai weivirus-like virus 4
Average proteome isoelectric point is 7.55
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KLC2|A0A1L3KLC2_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 4 OX=1922752 PE=4 SV=1
MM1 pKa = 7.44 LALRR5 pKa = 11.84 LGQTDD10 pKa = 3.69 RR11 pKa = 11.84 QIGEE15 pKa = 4.02 VAPFLLRR22 pKa = 11.84 HH23 pKa = 4.98 AAGRR27 pKa = 11.84 QGHH30 pKa = 5.21 WRR32 pKa = 11.84 ITAQGYY38 pKa = 7.54 DD39 pKa = 3.37 HH40 pKa = 7.68 PAAQSTKK47 pKa = 9.57 AYY49 pKa = 9.27 SVVYY53 pKa = 9.96 TEE55 pKa = 5.04 EE56 pKa = 4.21 EE57 pKa = 3.95 FSKK60 pKa = 10.69 LSRR63 pKa = 11.84 VVYY66 pKa = 10.29 HH67 pKa = 6.5 GLRR70 pKa = 11.84 NSTKK74 pKa = 8.63 PTSLHH79 pKa = 6.52 ASLEE83 pKa = 4.22 NVAQVLWPTGSPDD96 pKa = 3.32 HH97 pKa = 6.3 VAMRR101 pKa = 11.84 HH102 pKa = 4.55 VGVFVMMQYY111 pKa = 7.27 MTSDD115 pKa = 3.33 EE116 pKa = 4.38 TAVRR120 pKa = 11.84 PLARR124 pKa = 11.84 WAMVAGVGIGLMVRR138 pKa = 11.84 SVADD142 pKa = 3.52 GWKK145 pKa = 9.77 LATIAGLSPVLLARR159 pKa = 11.84 LVSRR163 pKa = 11.84 GRR165 pKa = 11.84 AIPILEE171 pKa = 3.98 RR172 pKa = 11.84 VGASQAAVAPNPGGSGPPPQAAPAPPPPPQGEE204 pKa = 4.18 PPPPGDD210 pKa = 3.62 AEE212 pKa = 4.05 TDD214 pKa = 3.02 EE215 pKa = 4.46 GVRR218 pKa = 11.84 VEE220 pKa = 4.66 EE221 pKa = 4.54 LNEE224 pKa = 4.03 EE225 pKa = 4.31 PPVIMDD231 pKa = 4.25 TPALPDD237 pKa = 3.93 DD238 pKa = 4.54 AATAAAASNDD248 pKa = 3.27 RR249 pKa = 11.84 RR250 pKa = 11.84 TMQVGDD256 pKa = 3.72 VVAVIGQAFNKK267 pKa = 10.1 DD268 pKa = 3.24 EE269 pKa = 4.81 PVNHH273 pKa = 6.04 MPVVGCLVGPCQVKK287 pKa = 9.9 PNVYY291 pKa = 10.71 AKK293 pKa = 9.47 TASNLKK299 pKa = 10.03 AAIEE303 pKa = 3.82 EE304 pKa = 4.64 RR305 pKa = 11.84 ITKK308 pKa = 10.09 KK309 pKa = 10.42 ARR311 pKa = 11.84 KK312 pKa = 8.42 CQLSKK317 pKa = 10.63 KK318 pKa = 9.74 DD319 pKa = 3.26 KK320 pKa = 10.75 ARR322 pKa = 11.84 IGGLVRR328 pKa = 11.84 QSMSCHH334 pKa = 5.6 RR335 pKa = 11.84 VRR337 pKa = 11.84 GVFSKK342 pKa = 10.87 RR343 pKa = 11.84 KK344 pKa = 7.73 IEE346 pKa = 3.6 AWAIEE351 pKa = 4.03 NLDD354 pKa = 3.86 LEE356 pKa = 5.28 LIRR359 pKa = 11.84 SGKK362 pKa = 8.94 WSVEE366 pKa = 3.67 RR367 pKa = 11.84 FRR369 pKa = 11.84 ASLEE373 pKa = 3.58 ALYY376 pKa = 10.81 ARR378 pKa = 11.84 EE379 pKa = 3.81 YY380 pKa = 9.16 PTYY383 pKa = 10.29 QFKK386 pKa = 11.08 AGIKK390 pKa = 7.88 PEE392 pKa = 4.04 CMAEE396 pKa = 4.19 GKK398 pKa = 10.24 APRR401 pKa = 11.84 MLIADD406 pKa = 4.1 GDD408 pKa = 4.23 DD409 pKa = 3.83 GQLMALAVVRR419 pKa = 11.84 CFEE422 pKa = 4.66 DD423 pKa = 3.6 LLFEE427 pKa = 4.22 HH428 pKa = 7.01 FEE430 pKa = 4.23 RR431 pKa = 11.84 KK432 pKa = 9.43 SIKK435 pKa = 10.43 HH436 pKa = 4.13 MAKK439 pKa = 9.73 RR440 pKa = 11.84 EE441 pKa = 3.97 AMDD444 pKa = 3.49 RR445 pKa = 11.84 VVKK448 pKa = 10.26 EE449 pKa = 4.07 LSKK452 pKa = 10.95 KK453 pKa = 8.46 GAKK456 pKa = 9.6 AVEE459 pKa = 4.46 GDD461 pKa = 3.29 GSAWDD466 pKa = 3.82 TTCNVEE472 pKa = 4.35 IRR474 pKa = 11.84 ALVEE478 pKa = 4.03 NPVLRR483 pKa = 11.84 HH484 pKa = 5.07 ICEE487 pKa = 4.22 VLCGLGVVPEE497 pKa = 4.05 SWLRR501 pKa = 11.84 EE502 pKa = 3.66 HH503 pKa = 7.25 SAACEE508 pKa = 3.96 QKK510 pKa = 9.31 TLRR513 pKa = 11.84 LFFKK517 pKa = 11.03 NKK519 pKa = 8.0 MEE521 pKa = 4.37 SMSVTIDD528 pKa = 3.9 AIRR531 pKa = 11.84 RR532 pKa = 11.84 SGHH535 pKa = 6.55 RR536 pKa = 11.84 GTSCLNWWVNFTLWVSSIFAEE557 pKa = 4.39 PEE559 pKa = 3.74 RR560 pKa = 11.84 FLDD563 pKa = 3.56 PDD565 pKa = 3.28 VRR567 pKa = 11.84 TGEE570 pKa = 4.5 DD571 pKa = 3.0 LTGQQRR577 pKa = 11.84 WWNGCFEE584 pKa = 5.17 GDD586 pKa = 5.31 DD587 pKa = 4.28 SLCTMKK593 pKa = 10.66 PPMEE597 pKa = 4.64 EE598 pKa = 3.82 GDD600 pKa = 3.52 RR601 pKa = 11.84 MSEE604 pKa = 3.59 IFLEE608 pKa = 4.92 FWRR611 pKa = 11.84 DD612 pKa = 2.68 AGFNMKK618 pKa = 9.64 IVFCTTRR625 pKa = 11.84 ATFVGWHH632 pKa = 6.47 IGCTDD637 pKa = 3.42 GEE639 pKa = 4.63 LNEE642 pKa = 4.39 HH643 pKa = 7.02 RR644 pKa = 11.84 CPEE647 pKa = 4.16 LPRR650 pKa = 11.84 ALANSGVSVSPQGVEE665 pKa = 3.5 AGRR668 pKa = 11.84 DD669 pKa = 3.47 GKK671 pKa = 11.25 LSVVKK676 pKa = 10.36 VLAAASALARR686 pKa = 11.84 ASDD689 pKa = 3.83 FSGILPSVSQKK700 pKa = 10.81 YY701 pKa = 10.55 LDD703 pKa = 3.81 FAEE706 pKa = 4.75 EE707 pKa = 4.46 CTTSDD712 pKa = 3.72 FEE714 pKa = 4.59 DD715 pKa = 4.51 RR716 pKa = 11.84 EE717 pKa = 3.84 MSYY720 pKa = 10.76 RR721 pKa = 11.84 AFGEE725 pKa = 4.07 AGYY728 pKa = 9.93 KK729 pKa = 10.6 ANDD732 pKa = 3.15 VRR734 pKa = 11.84 EE735 pKa = 4.06 QVQARR740 pKa = 11.84 NLEE743 pKa = 4.07 VTPEE747 pKa = 4.02 KK748 pKa = 10.57 EE749 pKa = 4.18 QEE751 pKa = 3.95 TLRR754 pKa = 11.84 ALGYY758 pKa = 10.07 QATHH762 pKa = 6.79 DD763 pKa = 4.08 EE764 pKa = 3.97 IMTFRR769 pKa = 11.84 EE770 pKa = 4.66 YY771 pKa = 10.1 IWSLEE776 pKa = 3.87 PAALVAYY783 pKa = 10.22 DD784 pKa = 3.97 SFRR787 pKa = 11.84 EE788 pKa = 4.01 SLPPSWRR795 pKa = 11.84 AAA797 pKa = 3.38
Molecular weight: 88.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.94
IPC2_protein 5.919
IPC_protein 5.97
Toseland 6.237
ProMoST 6.326
Dawson 6.237
Bjellqvist 6.249
Wikipedia 6.237
Rodwell 6.224
Grimsley 6.338
Solomon 6.237
Lehninger 6.224
Nozaki 6.504
DTASelect 6.678
Thurlkill 6.693
EMBOSS 6.649
Sillero 6.605
Patrickios 4.418
IPC_peptide 6.249
IPC2_peptide 6.561
IPC2.peptide.svr19 6.548
Protein with the highest isoelectric point:
>tr|A0A1L3KLC2|A0A1L3KLC2_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 4 OX=1922752 PE=4 SV=1
MM1 pKa = 7.46 TPNKK5 pKa = 9.1 PAQRR9 pKa = 11.84 NGRR12 pKa = 11.84 KK13 pKa = 9.4 LRR15 pKa = 11.84 DD16 pKa = 3.47 SGDD19 pKa = 3.16 KK20 pKa = 10.46 SKK22 pKa = 10.93 RR23 pKa = 11.84 PQRR26 pKa = 11.84 RR27 pKa = 11.84 RR28 pKa = 11.84 GGAASRR34 pKa = 11.84 THH36 pKa = 6.65 ANRR39 pKa = 11.84 VLATGVGNVPTKK51 pKa = 10.57 AWGSSRR57 pKa = 11.84 GGSLLCWDD65 pKa = 4.81 AKK67 pKa = 10.64 HH68 pKa = 6.04 PHH70 pKa = 6.55 HH71 pKa = 7.04 LSLPRR76 pKa = 11.84 AVGPYY81 pKa = 5.61 TTIRR85 pKa = 11.84 ATRR88 pKa = 11.84 RR89 pKa = 11.84 VATGRR94 pKa = 11.84 IANIIGTFKK103 pKa = 10.72 SRR105 pKa = 11.84 HH106 pKa = 5.19 NSSSLGEE113 pKa = 4.06 ASADD117 pKa = 3.98 TKK119 pKa = 10.01 WSEE122 pKa = 4.01 IIMISDD128 pKa = 3.52 HH129 pKa = 7.1 VSANPINGTDD139 pKa = 3.02 NTFFNNIDD147 pKa = 3.62 LGEE150 pKa = 4.52 LGSAATLVPSALTVQIVCPDD170 pKa = 3.2 NLAADD175 pKa = 4.1 GAKK178 pKa = 9.45 GVVYY182 pKa = 10.35 AGVMNTQAMVGGKK195 pKa = 9.42 SDD197 pKa = 3.24 SWDD200 pKa = 2.98 AYY202 pKa = 9.0 MNRR205 pKa = 11.84 FVQFQSPRR213 pKa = 11.84 MLAATKK219 pKa = 10.12 LALRR223 pKa = 11.84 GVTINSYY230 pKa = 9.59 PLDD233 pKa = 3.65 MSEE236 pKa = 5.15 VSDD239 pKa = 3.56 FTQLHH244 pKa = 5.22 EE245 pKa = 4.62 RR246 pKa = 11.84 EE247 pKa = 4.17 GLEE250 pKa = 3.96 YY251 pKa = 9.92 TGTMSSLSPQPAGWAPICIYY271 pKa = 10.56 NPQGALLEE279 pKa = 4.02 LLITVEE285 pKa = 3.69 YY286 pKa = 10.09 RR287 pKa = 11.84 VRR289 pKa = 11.84 FDD291 pKa = 4.25 LEE293 pKa = 4.24 HH294 pKa = 6.86 PASASHH300 pKa = 5.16 VHH302 pKa = 6.27 HH303 pKa = 6.96 PVASDD308 pKa = 3.62 STWDD312 pKa = 3.27 KK313 pKa = 10.14 LTRR316 pKa = 11.84 KK317 pKa = 9.76 ASSLGHH323 pKa = 5.59 GVMDD327 pKa = 4.11 IADD330 pKa = 3.62 VVANIGSAVRR340 pKa = 11.84 TGRR343 pKa = 11.84 QMLSAFGG350 pKa = 3.46
Molecular weight: 37.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.158
IPC2_protein 9.004
IPC_protein 9.048
Toseland 9.721
ProMoST 9.458
Dawson 9.955
Bjellqvist 9.677
Wikipedia 10.131
Rodwell 10.233
Grimsley 10.028
Solomon 9.999
Lehninger 9.97
Nozaki 9.794
DTASelect 9.648
Thurlkill 9.809
EMBOSS 10.145
Sillero 9.897
Patrickios 7.629
IPC_peptide 9.999
IPC2_peptide 8.39
IPC2.peptide.svr19 8.019
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1147
350
797
573.5
62.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.811 ± 0.133
1.744 ± 0.492
4.969 ± 0.096
6.452 ± 1.993
3.051 ± 0.425
7.672 ± 0.657
2.528 ± 0.499
3.836 ± 0.408
4.359 ± 0.358
7.759 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.964 ± 0.059
3.313 ± 0.856
5.754 ± 0.339
3.139 ± 0.156
7.323 ± 0.1
7.411 ± 1.277
5.231 ± 0.902
7.759 ± 0.5
2.005 ± 0.161
1.918 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here