Wobbly possum disease virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Arnidovirineae; Arteriviridae; Zealarterivirinae; Kappaarterivirus; Kappaarterivirus wobum

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9FGR9|G9FGR9_9NIDO Envelope protein OS=Wobbly possum disease virus OX=1118369 PE=4 SV=1
MM1 pKa = 7.34LNWRR5 pKa = 11.84RR6 pKa = 11.84FATALCTLYY15 pKa = 11.16AFLLVRR21 pKa = 11.84TAADD25 pKa = 3.87TPDD28 pKa = 3.48PSYY31 pKa = 11.17QYY33 pKa = 10.12VHH35 pKa = 6.25AHH37 pKa = 6.38ISSCAKK43 pKa = 10.07IFQQLDD49 pKa = 2.76IDD51 pKa = 4.93FNQSLPVMLADD62 pKa = 5.98LIEE65 pKa = 4.54QPSHH69 pKa = 6.21WLSTHH74 pKa = 6.31PGVVRR79 pKa = 11.84AFDD82 pKa = 4.98EE83 pKa = 4.0IHH85 pKa = 6.61DD86 pKa = 4.34TYY88 pKa = 11.51TLATSYY94 pKa = 11.5YY95 pKa = 9.07EE96 pKa = 4.6LPHH99 pKa = 8.12SYY101 pKa = 9.76ILHH104 pKa = 5.97EE105 pKa = 4.06MLGNAEE111 pKa = 4.51AVLEE115 pKa = 3.98PLRR118 pKa = 11.84YY119 pKa = 9.05YY120 pKa = 9.18RR121 pKa = 11.84THH123 pKa = 6.24FRR125 pKa = 11.84YY126 pKa = 9.79LDD128 pKa = 3.4RR129 pKa = 11.84MALEE133 pKa = 4.47SLAHH137 pKa = 6.26LSYY140 pKa = 11.03IKK142 pKa = 10.43VQQDD146 pKa = 2.93NDD148 pKa = 3.66TLANATFTIHH158 pKa = 7.52PNIQWNEE165 pKa = 3.68LQFAGVVPSPFYY177 pKa = 9.98MVYY180 pKa = 10.37MSMACWLITIHH191 pKa = 6.95AFLRR195 pKa = 11.84LRR197 pKa = 11.84FQWILCATSRR207 pKa = 4.2

Molecular weight: 24.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9FGS4|G9FGS4_9NIDO Membrane protein OS=Wobbly possum disease virus OX=1118369 PE=4 SV=1
MM1 pKa = 6.56QTSPWSLDD9 pKa = 3.14TKK11 pKa = 11.26LMLGRR16 pKa = 11.84KK17 pKa = 7.45MVLMLKK23 pKa = 10.36LLRR26 pKa = 11.84SSSHH30 pKa = 6.56RR31 pKa = 11.84LHH33 pKa = 7.1PCHH36 pKa = 6.44TSHH39 pKa = 7.83WDD41 pKa = 3.31LTPSRR46 pKa = 11.84GCRR49 pKa = 11.84CCIIITRR56 pKa = 11.84LTIASACAYY65 pKa = 6.77TSPRR69 pKa = 11.84IFATRR74 pKa = 11.84RR75 pKa = 11.84SLRR78 pKa = 3.5

Molecular weight: 8.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

6514

45

3402

651.4

71.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.612 ± 0.437

2.871 ± 0.264

4.421 ± 0.513

3.485 ± 0.387

4.206 ± 0.161

6.954 ± 0.575

2.702 ± 0.738

4.774 ± 0.346

3.915 ± 0.474

9.18 ± 0.819

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.251

3.669 ± 0.286

6.739 ± 0.798

2.379 ± 0.203

4.943 ± 0.61

7.169 ± 0.316

7.016 ± 0.617

9.426 ± 1.054

1.274 ± 0.181

3.961 ± 0.412
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski