Giant house spider associated circular virus 2
Average proteome isoelectric point is 8.11
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPD7|A0A346BPD7_9VIRU Putative capsid protein OS=Giant house spider associated circular virus 2 OX=2293289 PE=4 SV=1
MM1 pKa = 7.42 PTPKK5 pKa = 10.02 KK6 pKa = 10.43 AYY8 pKa = 10.23 CFTLNNYY15 pKa = 7.76 TSEE18 pKa = 4.46 EE19 pKa = 4.12 YY20 pKa = 10.9 EE21 pKa = 4.36 EE22 pKa = 4.46 ICGKK26 pKa = 10.71 LSDD29 pKa = 4.23 DD30 pKa = 4.03 CLYY33 pKa = 11.06 AIIGKK38 pKa = 8.81 EE39 pKa = 4.02 VGEE42 pKa = 4.5 SGTPHH47 pKa = 5.98 LQGYY51 pKa = 8.65 CLFKK55 pKa = 10.66 RR56 pKa = 11.84 ALQFNTIKK64 pKa = 10.54 NRR66 pKa = 11.84 YY67 pKa = 8.44 LPRR70 pKa = 11.84 CHH72 pKa = 7.6 IEE74 pKa = 4.05 VAAGSADD81 pKa = 3.43 ANRR84 pKa = 11.84 AYY86 pKa = 10.35 CSKK89 pKa = 10.11 GGSFIEE95 pKa = 4.76 YY96 pKa = 7.13 GTKK99 pKa = 9.76 PKK101 pKa = 10.51 KK102 pKa = 10.59 SNTRR106 pKa = 11.84 SEE108 pKa = 4.12 LASAFCTHH116 pKa = 6.56 MGSGRR121 pKa = 11.84 SGMVQFAAEE130 pKa = 4.61 EE131 pKa = 4.18 PGTWYY136 pKa = 10.7 FSGHH140 pKa = 5.45 NLLRR144 pKa = 11.84 NYY146 pKa = 10.41 LAIQPPISRR155 pKa = 11.84 DD156 pKa = 3.49 AISVKK161 pKa = 9.53 WYY163 pKa = 9.57 YY164 pKa = 11.06 GEE166 pKa = 4.41 PGVGKK171 pKa = 9.62 SRR173 pKa = 11.84 RR174 pKa = 11.84 AHH176 pKa = 5.98 MEE178 pKa = 3.81 LPDD181 pKa = 4.02 AFIKK185 pKa = 10.43 EE186 pKa = 4.54 PRR188 pKa = 11.84 TKK190 pKa = 8.81 WWSGYY195 pKa = 10.37 ALEE198 pKa = 4.67 TDD200 pKa = 4.43 VIIDD204 pKa = 3.64 DD205 pKa = 4.85 FGPNGIDD212 pKa = 3.32 INHH215 pKa = 7.46 LLRR218 pKa = 11.84 WFDD221 pKa = 3.35 RR222 pKa = 11.84 YY223 pKa = 10.95 KK224 pKa = 10.88 CVVEE228 pKa = 4.57 SKK230 pKa = 11.15 GGMLPLYY237 pKa = 9.77 AVNFIVTSNFHH248 pKa = 6.81 PNQIFQFGGEE258 pKa = 4.39 LNAQLPALMRR268 pKa = 11.84 RR269 pKa = 11.84 IEE271 pKa = 4.64 CIEE274 pKa = 3.8 MM275 pKa = 4.13
Molecular weight: 31.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.925
IPC2_protein 7.088
IPC_protein 7.044
Toseland 6.766
ProMoST 7.527
Dawson 7.702
Bjellqvist 8.009
Wikipedia 7.614
Rodwell 7.702
Grimsley 6.81
Solomon 7.761
Lehninger 7.775
Nozaki 8.244
DTASelect 7.863
Thurlkill 7.892
EMBOSS 7.936
Sillero 8.214
Patrickios 3.757
IPC_peptide 7.761
IPC2_peptide 7.38
IPC2.peptide.svr19 7.276
Protein with the highest isoelectric point:
>tr|A0A346BPD7|A0A346BPD7_9VIRU Putative capsid protein OS=Giant house spider associated circular virus 2 OX=2293289 PE=4 SV=1
MM1 pKa = 7.76 AGRR4 pKa = 11.84 KK5 pKa = 8.54 RR6 pKa = 11.84 VYY8 pKa = 10.89 AMPVSKK14 pKa = 10.66 SNRR17 pKa = 11.84 PFKK20 pKa = 10.38 KK21 pKa = 9.77 RR22 pKa = 11.84 RR23 pKa = 11.84 ISRR26 pKa = 11.84 RR27 pKa = 11.84 KK28 pKa = 8.83 RR29 pKa = 11.84 RR30 pKa = 11.84 GSRR33 pKa = 11.84 KK34 pKa = 9.32 SVNFTSQSGRR44 pKa = 11.84 GTGLGFSRR52 pKa = 11.84 KK53 pKa = 7.95 RR54 pKa = 11.84 TSLRR58 pKa = 11.84 KK59 pKa = 9.66 FKK61 pKa = 10.82 RR62 pKa = 11.84 LLWDD66 pKa = 3.11 SSTAQTHH73 pKa = 4.82 YY74 pKa = 10.51 RR75 pKa = 11.84 SNFAFTTAINTTAVASTMSVTVIASRR101 pKa = 11.84 RR102 pKa = 11.84 FNNQNFWTTAGGAINPDD119 pKa = 3.49 GGTIPTFVTNGDD131 pKa = 3.37 FTIRR135 pKa = 11.84 GGMYY139 pKa = 10.7 GLRR142 pKa = 11.84 LCNAPDD148 pKa = 4.27 AADD151 pKa = 3.66 TDD153 pKa = 4.02 KK154 pKa = 11.57 DD155 pKa = 3.99 AIQCIVYY162 pKa = 9.93 LVKK165 pKa = 9.97 STKK168 pKa = 9.42 NWNSTNLPASVPVGFDD184 pKa = 3.15 PSLVQDD190 pKa = 4.13 FQTNIGKK197 pKa = 9.03 IVYY200 pKa = 9.63 KK201 pKa = 10.69 KK202 pKa = 10.67 EE203 pKa = 3.67 FLLEE207 pKa = 4.09 DD208 pKa = 3.62 TNVAVIEE215 pKa = 4.11 KK216 pKa = 10.51 RR217 pKa = 11.84 MGLSKK222 pKa = 10.11 IDD224 pKa = 3.08 ISEE227 pKa = 4.25 YY228 pKa = 10.58 QNSQSEE234 pKa = 4.58 FVWIILTGIVSGTTIKK250 pKa = 10.83 GVTVVPYY257 pKa = 10.61 YY258 pKa = 10.74 NLSFVGDD265 pKa = 3.96 TVV267 pKa = 3.14
Molecular weight: 29.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.75
IPC_protein 10.248
Toseland 10.628
ProMoST 10.233
Dawson 10.745
Bjellqvist 10.394
Wikipedia 10.906
Rodwell 11.125
Grimsley 10.789
Solomon 10.804
Lehninger 10.774
Nozaki 10.599
DTASelect 10.394
Thurlkill 10.628
EMBOSS 11.023
Sillero 10.657
Patrickios 10.833
IPC_peptide 10.804
IPC2_peptide 9.151
IPC2.peptide.svr19 8.647
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
542
267
275
271.0
30.37
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.642 ± 0.452
2.03 ± 0.892
4.059 ± 0.303
4.428 ± 1.78
5.351 ± 0.186
8.303 ± 0.305
1.476 ± 0.767
6.458 ± 0.063
6.273 ± 0.326
6.273 ± 0.717
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.214 ± 0.238
5.72 ± 0.451
4.428 ± 0.736
2.768 ± 0.159
6.642 ± 0.852
7.934 ± 0.996
7.196 ± 2.031
5.904 ± 1.626
1.661 ± 0.113
4.244 ± 0.869
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here