Festuca pratensis amalgavirus 1
Average proteome isoelectric point is 6.56
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6JIM9|A0A2Z6JIM9_9VIRU ORF1p OS=Festuca pratensis amalgavirus 1 OX=2069324 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.65 AEE3 pKa = 4.18 VKK5 pKa = 9.53 RR6 pKa = 11.84 TFQTPAPTDD15 pKa = 3.02 GDD17 pKa = 3.93 YY18 pKa = 11.33 AANLPEE24 pKa = 5.8 GDD26 pKa = 3.77 DD27 pKa = 3.63 ARR29 pKa = 11.84 FVYY32 pKa = 9.99 YY33 pKa = 10.57 AGWVLTTYY41 pKa = 10.54 HH42 pKa = 6.54 FAAALFQPGTYY53 pKa = 9.68 RR54 pKa = 11.84 LEE56 pKa = 4.61 GYY58 pKa = 7.78 TDD60 pKa = 3.35 KK61 pKa = 11.51 DD62 pKa = 3.27 FAARR66 pKa = 11.84 LRR68 pKa = 11.84 YY69 pKa = 9.52 FKK71 pKa = 11.12 GKK73 pKa = 9.62 DD74 pKa = 3.16 VNVIEE79 pKa = 4.88 TIVAVGIRR87 pKa = 11.84 RR88 pKa = 11.84 NFFTAADD95 pKa = 3.59 SATFEE100 pKa = 4.03 NFANFLEE107 pKa = 4.41 FLKK110 pKa = 10.21 TPEE113 pKa = 4.1 GASAITEE120 pKa = 4.07 DD121 pKa = 3.54 LRR123 pKa = 11.84 RR124 pKa = 11.84 ARR126 pKa = 11.84 FLAAGKK132 pKa = 10.46 GVFTAVDD139 pKa = 3.68 IAAVSQLTVQAADD152 pKa = 3.98 LEE154 pKa = 4.45 QHH156 pKa = 6.39 KK157 pKa = 10.9 ARR159 pKa = 11.84 MIAASQKK166 pKa = 10.7 RR167 pKa = 11.84 IDD169 pKa = 4.05 EE170 pKa = 4.54 LEE172 pKa = 3.83 LAIAKK177 pKa = 9.56 EE178 pKa = 4.2 KK179 pKa = 10.92 EE180 pKa = 4.11 SLEE183 pKa = 4.25 TKK185 pKa = 10.44 LALSKK190 pKa = 11.48 DD191 pKa = 3.64 EE192 pKa = 4.88 FFPASIYY199 pKa = 10.49 KK200 pKa = 10.1 KK201 pKa = 9.22 PNAMKK206 pKa = 10.22 LQKK209 pKa = 10.35 EE210 pKa = 4.36 CLIKK214 pKa = 10.77 AKK216 pKa = 10.48 AADD219 pKa = 3.83 PVLSAVAVPTNEE231 pKa = 4.3 QISDD235 pKa = 3.37 ALKK238 pKa = 9.49 QFRR241 pKa = 11.84 EE242 pKa = 4.06 EE243 pKa = 4.09 VEE245 pKa = 4.09 RR246 pKa = 11.84 QDD248 pKa = 3.3 MVTFLSQQDD257 pKa = 3.3 RR258 pKa = 11.84 LHH260 pKa = 6.95 RR261 pKa = 11.84 LRR263 pKa = 11.84 EE264 pKa = 4.01 YY265 pKa = 10.5 VGKK268 pKa = 10.66 KK269 pKa = 9.12 ILSFRR274 pKa = 11.84 ACGKK278 pKa = 10.32 RR279 pKa = 11.84 KK280 pKa = 9.42 ISDD283 pKa = 3.57 RR284 pKa = 11.84 LVHH287 pKa = 6.17 VLAAMDD293 pKa = 3.91 GQLTDD298 pKa = 4.89 EE299 pKa = 4.85 IPAFGAGVGDD309 pKa = 5.23 DD310 pKa = 4.32 NDD312 pKa = 4.35 SDD314 pKa = 4.19 GDD316 pKa = 4.09 DD317 pKa = 3.64 YY318 pKa = 11.98 VPEE321 pKa = 4.3 YY322 pKa = 10.49 EE323 pKa = 6.22 LEE325 pKa = 4.24 DD326 pKa = 3.7 QEE328 pKa = 4.49 EE329 pKa = 4.52 SSGGSEE335 pKa = 4.51 GAGDD339 pKa = 4.23 EE340 pKa = 4.28 QAEE343 pKa = 4.52 DD344 pKa = 3.97 PPIISPLQTRR354 pKa = 11.84 RR355 pKa = 11.84 QRR357 pKa = 11.84 AARR360 pKa = 11.84 AGRR363 pKa = 11.84 GSSATGRR370 pKa = 11.84 GATSRR375 pKa = 11.84 ATRR378 pKa = 11.84 ARR380 pKa = 11.84 RR381 pKa = 11.84 AA382 pKa = 3.28
Molecular weight: 42.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.4
IPC2_protein 5.461
IPC_protein 5.41
Toseland 5.423
ProMoST 5.575
Dawson 5.436
Bjellqvist 5.55
Wikipedia 5.321
Rodwell 5.359
Grimsley 5.372
Solomon 5.436
Lehninger 5.397
Nozaki 5.575
DTASelect 5.741
Thurlkill 5.448
EMBOSS 5.41
Sillero 5.652
Patrickios 4.533
IPC_peptide 5.448
IPC2_peptide 5.639
IPC2.peptide.svr19 5.755
Protein with the highest isoelectric point:
>tr|A0A2Z6JIM9|A0A2Z6JIM9_9VIRU ORF1p OS=Festuca pratensis amalgavirus 1 OX=2069324 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.65 AEE3 pKa = 4.18 VKK5 pKa = 9.53 RR6 pKa = 11.84 TFQTPAPTDD15 pKa = 3.02 GDD17 pKa = 3.93 YY18 pKa = 11.33 AANLPEE24 pKa = 5.8 GDD26 pKa = 3.77 DD27 pKa = 3.63 ARR29 pKa = 11.84 FVYY32 pKa = 9.99 YY33 pKa = 10.57 AGWVLTTYY41 pKa = 10.54 HH42 pKa = 6.54 FAAALFQPGTYY53 pKa = 9.68 RR54 pKa = 11.84 LEE56 pKa = 4.61 GYY58 pKa = 7.78 TDD60 pKa = 3.35 KK61 pKa = 11.51 DD62 pKa = 3.27 FAARR66 pKa = 11.84 LRR68 pKa = 11.84 YY69 pKa = 9.52 FKK71 pKa = 11.12 GKK73 pKa = 9.62 DD74 pKa = 3.16 VNVIEE79 pKa = 4.88 TIVAVGIRR87 pKa = 11.84 RR88 pKa = 11.84 NFFTAADD95 pKa = 3.59 SATFEE100 pKa = 4.03 NFANFLEE107 pKa = 4.41 FLKK110 pKa = 10.21 TPEE113 pKa = 4.1 GASAITEE120 pKa = 4.07 DD121 pKa = 3.54 LRR123 pKa = 11.84 RR124 pKa = 11.84 ARR126 pKa = 11.84 FLAAGKK132 pKa = 10.46 GVFTAVDD139 pKa = 3.68 IAAVSQLTVQAADD152 pKa = 3.98 LEE154 pKa = 4.45 QHH156 pKa = 6.39 KK157 pKa = 10.9 ARR159 pKa = 11.84 MIAASQKK166 pKa = 10.7 RR167 pKa = 11.84 IDD169 pKa = 4.05 EE170 pKa = 4.54 LEE172 pKa = 3.83 LAIAKK177 pKa = 9.56 EE178 pKa = 4.2 KK179 pKa = 10.92 EE180 pKa = 4.11 SLEE183 pKa = 4.25 TKK185 pKa = 10.44 LALSKK190 pKa = 11.48 DD191 pKa = 3.64 EE192 pKa = 4.88 FFPASIYY199 pKa = 10.49 KK200 pKa = 10.1 KK201 pKa = 9.22 PNAMKK206 pKa = 10.22 LQKK209 pKa = 10.35 EE210 pKa = 4.36 CLIKK214 pKa = 10.77 AKK216 pKa = 10.48 AADD219 pKa = 3.83 PVLSAVAVPTNEE231 pKa = 4.3 QISDD235 pKa = 3.37 ALKK238 pKa = 9.49 QFRR241 pKa = 11.84 EE242 pKa = 4.06 EE243 pKa = 4.09 VEE245 pKa = 4.09 RR246 pKa = 11.84 QDD248 pKa = 3.3 MVTFLSQQDD257 pKa = 3.3 RR258 pKa = 11.84 LHH260 pKa = 6.95 RR261 pKa = 11.84 LRR263 pKa = 11.84 EE264 pKa = 4.01 YY265 pKa = 10.5 VGKK268 pKa = 10.59 KK269 pKa = 9.19 ILSFEE274 pKa = 4.18 LVEE277 pKa = 4.25 SGRR280 pKa = 11.84 YY281 pKa = 9.11 PIDD284 pKa = 4.99 LSTSWLPWTASLLMKK299 pKa = 10.55 YY300 pKa = 9.47 PLSEE304 pKa = 3.98 RR305 pKa = 11.84 VSATIMIPMGTITFPSMSLKK325 pKa = 9.88 IRR327 pKa = 11.84 RR328 pKa = 11.84 NLLEE332 pKa = 3.97 VLKK335 pKa = 10.83 VPGMNRR341 pKa = 11.84 RR342 pKa = 11.84 KK343 pKa = 9.52 ILPSSAPSRR352 pKa = 11.84 LGASVPRR359 pKa = 11.84 AQGVAPLLQGGARR372 pKa = 11.84 LRR374 pKa = 11.84 VLRR377 pKa = 11.84 APVAPDD383 pKa = 3.09 TRR385 pKa = 11.84 GIPFARR391 pKa = 11.84 SKK393 pKa = 10.47 WEE395 pKa = 3.77 AGVRR399 pKa = 11.84 KK400 pKa = 9.81 IIGGGEE406 pKa = 4.02 MNDD409 pKa = 3.16 WLQVSSKK416 pKa = 9.55 EE417 pKa = 4.05 RR418 pKa = 11.84 CGGNASDD425 pKa = 4.73 ALLMLADD432 pKa = 4.39 ASDD435 pKa = 3.91 RR436 pKa = 11.84 LPGRR440 pKa = 11.84 ILRR443 pKa = 11.84 GLFTLRR449 pKa = 11.84 GARR452 pKa = 11.84 EE453 pKa = 4.05 ALRR456 pKa = 11.84 LPSGLSVPDD465 pKa = 4.16 GRR467 pKa = 11.84 ACCNVKK473 pKa = 10.16 HH474 pKa = 6.17 FNVEE478 pKa = 3.59 ATAGPFLRR486 pKa = 11.84 AFGVKK491 pKa = 9.73 KK492 pKa = 10.23 KK493 pKa = 10.55 AALEE497 pKa = 3.83 QLLGDD502 pKa = 4.21 FVWDD506 pKa = 4.22 CFDD509 pKa = 5.64 DD510 pKa = 4.26 FASNDD515 pKa = 2.82 GDD517 pKa = 3.65 ARR519 pKa = 11.84 RR520 pKa = 11.84 LPFFGMRR527 pKa = 11.84 IGFRR531 pKa = 11.84 TKK533 pKa = 10.43 LLPRR537 pKa = 11.84 SEE539 pKa = 4.5 MLTKK543 pKa = 10.43 IKK545 pKa = 10.42 DD546 pKa = 3.82 FKK548 pKa = 11.0 PLGRR552 pKa = 11.84 CVMMLDD558 pKa = 5.12 AIEE561 pKa = 4.41 QFCSSPLYY569 pKa = 10.69 NILSNLSADD578 pKa = 3.72 ALKK581 pKa = 10.97 DD582 pKa = 3.47 PLSGFRR588 pKa = 11.84 NTAVRR593 pKa = 11.84 ASSDD597 pKa = 2.64 WSYY600 pKa = 9.94 MWEE603 pKa = 4.11 EE604 pKa = 3.63 IKK606 pKa = 10.32 QAKK609 pKa = 9.22 VCMEE613 pKa = 4.52 LDD615 pKa = 2.81 WSKK618 pKa = 10.93 FDD620 pKa = 4.34 RR621 pKa = 11.84 EE622 pKa = 4.12 RR623 pKa = 11.84 PRR625 pKa = 11.84 DD626 pKa = 3.75 DD627 pKa = 5.03 LEE629 pKa = 4.93 FIVDD633 pKa = 3.9 LVISCFTPRR642 pKa = 11.84 TEE644 pKa = 4.2 RR645 pKa = 11.84 EE646 pKa = 3.69 QRR648 pKa = 11.84 LLRR651 pKa = 11.84 AYY653 pKa = 7.36 EE654 pKa = 3.78 ICMKK658 pKa = 10.24 RR659 pKa = 11.84 AIVEE663 pKa = 4.07 RR664 pKa = 11.84 VAILDD669 pKa = 4.0 DD670 pKa = 4.26 GALFTIDD677 pKa = 3.62 GMVPSGSLWTGWIDD691 pKa = 3.2 TALNILYY698 pKa = 10.47 LSAALNQAGFSLDD711 pKa = 3.34 TARR714 pKa = 11.84 PKK716 pKa = 10.84 CAGDD720 pKa = 3.88 DD721 pKa = 3.71 NLTLFLEE728 pKa = 4.76 DD729 pKa = 3.4 VTDD732 pKa = 3.37 SRR734 pKa = 11.84 LFYY737 pKa = 11.23 VRR739 pKa = 11.84 DD740 pKa = 3.66 LLNEE744 pKa = 3.88 WFLAGIKK751 pKa = 10.28 EE752 pKa = 3.93 KK753 pKa = 10.85 DD754 pKa = 3.71 FNITRR759 pKa = 11.84 PPFYY763 pKa = 10.83 VEE765 pKa = 4.55 TYY767 pKa = 10.08 QAVFPPGTDD776 pKa = 3.27 LSLGTSKK783 pKa = 10.49 IVDD786 pKa = 3.19 QCEE789 pKa = 3.71 WVRR792 pKa = 11.84 FEE794 pKa = 3.86 GQLYY798 pKa = 8.99 INQEE802 pKa = 4.19 EE803 pKa = 4.79 GHH805 pKa = 4.64 SHH807 pKa = 5.64 RR808 pKa = 11.84 WQYY811 pKa = 10.64 RR812 pKa = 11.84 FKK814 pKa = 10.9 GKK816 pKa = 9.57 PKK818 pKa = 10.28 FLSCYY823 pKa = 7.99 WLQDD827 pKa = 3.25 GRR829 pKa = 11.84 PIRR832 pKa = 11.84 PASDD836 pKa = 3.2 NVEE839 pKa = 3.77 KK840 pKa = 10.96 LLFPEE845 pKa = 6.28 GIHH848 pKa = 7.15 KK849 pKa = 10.8 DD850 pKa = 2.93 IDD852 pKa = 3.86 DD853 pKa = 4.57 YY854 pKa = 11.49 IAAVLAMVVDD864 pKa = 4.12 NPFNQHH870 pKa = 5.29 NVNHH874 pKa = 6.16 MKK876 pKa = 10.09 HH877 pKa = 6.55 RR878 pKa = 11.84 YY879 pKa = 9.24 LIAQQIKK886 pKa = 8.71 RR887 pKa = 11.84 VMVSTIPYY895 pKa = 9.7 QLILALSRR903 pKa = 11.84 IRR905 pKa = 11.84 SEE907 pKa = 4.04 AGEE910 pKa = 4.96 DD911 pKa = 3.27 IPFPMIAPWRR921 pKa = 11.84 RR922 pKa = 11.84 FKK924 pKa = 10.98 EE925 pKa = 4.07 WIDD928 pKa = 3.41 LDD930 pKa = 3.85 KK931 pKa = 11.31 YY932 pKa = 10.95 PPVQEE937 pKa = 4.62 WIRR940 pKa = 11.84 DD941 pKa = 3.4 FDD943 pKa = 4.41 DD944 pKa = 4.63 FVAGISGLYY953 pKa = 9.33 VRR955 pKa = 11.84 STTGGIDD962 pKa = 2.88 AYY964 pKa = 10.26 KK965 pKa = 10.72 FMDD968 pKa = 5.37 FIRR971 pKa = 11.84 GDD973 pKa = 3.74 SVIGEE978 pKa = 4.37 GQWGNEE984 pKa = 3.58 MTRR987 pKa = 11.84 WIRR990 pKa = 11.84 FVTEE994 pKa = 4.24 HH995 pKa = 6.15 PVSRR999 pKa = 11.84 ALRR1002 pKa = 11.84 KK1003 pKa = 9.42 ARR1005 pKa = 11.84 RR1006 pKa = 11.84 FNPKK1010 pKa = 9.44 AAPTAGEE1017 pKa = 3.82 NLFYY1021 pKa = 10.87 TRR1023 pKa = 11.84 AQGGLNAYY1031 pKa = 9.38 RR1032 pKa = 11.84 RR1033 pKa = 11.84 QLQNHH1038 pKa = 5.79 RR1039 pKa = 11.84 LEE1041 pKa = 4.33 SSEE1044 pKa = 5.37 EE1045 pKa = 3.77 YY1046 pKa = 10.85 GLWVSDD1052 pKa = 4.98 LLRR1055 pKa = 11.84 QKK1057 pKa = 10.96
Molecular weight: 119.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.717
IPC2_protein 8.009
IPC_protein 7.849
Toseland 7.819
ProMoST 8.492
Dawson 8.682
Bjellqvist 8.843
Wikipedia 8.697
Rodwell 8.77
Grimsley 7.834
Solomon 8.756
Lehninger 8.741
Nozaki 8.975
DTASelect 8.639
Thurlkill 8.726
EMBOSS 8.858
Sillero 8.975
Patrickios 4.724
IPC_peptide 8.741
IPC2_peptide 7.571
IPC2.peptide.svr19 7.821
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1439
382
1057
719.5
80.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.771 ± 1.778
1.042 ± 0.254
6.81 ± 0.383
6.463 ± 0.682
5.49 ± 0.125
6.254 ± 0.142
1.112 ± 0.032
5.073 ± 0.434
5.629 ± 0.192
9.521 ± 0.946
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.085 ± 0.38
2.849 ± 0.37
4.656 ± 0.486
3.683 ± 0.376
8.061 ± 0.102
5.698 ± 0.227
4.795 ± 0.473
5.629 ± 0.065
1.529 ± 0.621
2.849 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here