Festuca pratensis amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2Z6JIM9|A0A2Z6JIM9_9VIRU ORF1p OS=Festuca pratensis amalgavirus 1 OX=2069324 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.65AEE3 pKa = 4.18VKK5 pKa = 9.53RR6 pKa = 11.84TFQTPAPTDD15 pKa = 3.02GDD17 pKa = 3.93YY18 pKa = 11.33AANLPEE24 pKa = 5.8GDD26 pKa = 3.77DD27 pKa = 3.63ARR29 pKa = 11.84FVYY32 pKa = 9.99YY33 pKa = 10.57AGWVLTTYY41 pKa = 10.54HH42 pKa = 6.54FAAALFQPGTYY53 pKa = 9.68RR54 pKa = 11.84LEE56 pKa = 4.61GYY58 pKa = 7.78TDD60 pKa = 3.35KK61 pKa = 11.51DD62 pKa = 3.27FAARR66 pKa = 11.84LRR68 pKa = 11.84YY69 pKa = 9.52FKK71 pKa = 11.12GKK73 pKa = 9.62DD74 pKa = 3.16VNVIEE79 pKa = 4.88TIVAVGIRR87 pKa = 11.84RR88 pKa = 11.84NFFTAADD95 pKa = 3.59SATFEE100 pKa = 4.03NFANFLEE107 pKa = 4.41FLKK110 pKa = 10.21TPEE113 pKa = 4.1GASAITEE120 pKa = 4.07DD121 pKa = 3.54LRR123 pKa = 11.84RR124 pKa = 11.84ARR126 pKa = 11.84FLAAGKK132 pKa = 10.46GVFTAVDD139 pKa = 3.68IAAVSQLTVQAADD152 pKa = 3.98LEE154 pKa = 4.45QHH156 pKa = 6.39KK157 pKa = 10.9ARR159 pKa = 11.84MIAASQKK166 pKa = 10.7RR167 pKa = 11.84IDD169 pKa = 4.05EE170 pKa = 4.54LEE172 pKa = 3.83LAIAKK177 pKa = 9.56EE178 pKa = 4.2KK179 pKa = 10.92EE180 pKa = 4.11SLEE183 pKa = 4.25TKK185 pKa = 10.44LALSKK190 pKa = 11.48DD191 pKa = 3.64EE192 pKa = 4.88FFPASIYY199 pKa = 10.49KK200 pKa = 10.1KK201 pKa = 9.22PNAMKK206 pKa = 10.22LQKK209 pKa = 10.35EE210 pKa = 4.36CLIKK214 pKa = 10.77AKK216 pKa = 10.48AADD219 pKa = 3.83PVLSAVAVPTNEE231 pKa = 4.3QISDD235 pKa = 3.37ALKK238 pKa = 9.49QFRR241 pKa = 11.84EE242 pKa = 4.06EE243 pKa = 4.09VEE245 pKa = 4.09RR246 pKa = 11.84QDD248 pKa = 3.3MVTFLSQQDD257 pKa = 3.3RR258 pKa = 11.84LHH260 pKa = 6.95RR261 pKa = 11.84LRR263 pKa = 11.84EE264 pKa = 4.01YY265 pKa = 10.5VGKK268 pKa = 10.66KK269 pKa = 9.12ILSFRR274 pKa = 11.84ACGKK278 pKa = 10.32RR279 pKa = 11.84KK280 pKa = 9.42ISDD283 pKa = 3.57RR284 pKa = 11.84LVHH287 pKa = 6.17VLAAMDD293 pKa = 3.91GQLTDD298 pKa = 4.89EE299 pKa = 4.85IPAFGAGVGDD309 pKa = 5.23DD310 pKa = 4.32NDD312 pKa = 4.35SDD314 pKa = 4.19GDD316 pKa = 4.09DD317 pKa = 3.64YY318 pKa = 11.98VPEE321 pKa = 4.3YY322 pKa = 10.49EE323 pKa = 6.22LEE325 pKa = 4.24DD326 pKa = 3.7QEE328 pKa = 4.49EE329 pKa = 4.52SSGGSEE335 pKa = 4.51GAGDD339 pKa = 4.23EE340 pKa = 4.28QAEE343 pKa = 4.52DD344 pKa = 3.97PPIISPLQTRR354 pKa = 11.84RR355 pKa = 11.84QRR357 pKa = 11.84AARR360 pKa = 11.84AGRR363 pKa = 11.84GSSATGRR370 pKa = 11.84GATSRR375 pKa = 11.84ATRR378 pKa = 11.84ARR380 pKa = 11.84RR381 pKa = 11.84AA382 pKa = 3.28

Molecular weight:
42.12 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2Z6JIM9|A0A2Z6JIM9_9VIRU ORF1p OS=Festuca pratensis amalgavirus 1 OX=2069324 GN=ORF1 PE=4 SV=1
MM1 pKa = 7.65AEE3 pKa = 4.18VKK5 pKa = 9.53RR6 pKa = 11.84TFQTPAPTDD15 pKa = 3.02GDD17 pKa = 3.93YY18 pKa = 11.33AANLPEE24 pKa = 5.8GDD26 pKa = 3.77DD27 pKa = 3.63ARR29 pKa = 11.84FVYY32 pKa = 9.99YY33 pKa = 10.57AGWVLTTYY41 pKa = 10.54HH42 pKa = 6.54FAAALFQPGTYY53 pKa = 9.68RR54 pKa = 11.84LEE56 pKa = 4.61GYY58 pKa = 7.78TDD60 pKa = 3.35KK61 pKa = 11.51DD62 pKa = 3.27FAARR66 pKa = 11.84LRR68 pKa = 11.84YY69 pKa = 9.52FKK71 pKa = 11.12GKK73 pKa = 9.62DD74 pKa = 3.16VNVIEE79 pKa = 4.88TIVAVGIRR87 pKa = 11.84RR88 pKa = 11.84NFFTAADD95 pKa = 3.59SATFEE100 pKa = 4.03NFANFLEE107 pKa = 4.41FLKK110 pKa = 10.21TPEE113 pKa = 4.1GASAITEE120 pKa = 4.07DD121 pKa = 3.54LRR123 pKa = 11.84RR124 pKa = 11.84ARR126 pKa = 11.84FLAAGKK132 pKa = 10.46GVFTAVDD139 pKa = 3.68IAAVSQLTVQAADD152 pKa = 3.98LEE154 pKa = 4.45QHH156 pKa = 6.39KK157 pKa = 10.9ARR159 pKa = 11.84MIAASQKK166 pKa = 10.7RR167 pKa = 11.84IDD169 pKa = 4.05EE170 pKa = 4.54LEE172 pKa = 3.83LAIAKK177 pKa = 9.56EE178 pKa = 4.2KK179 pKa = 10.92EE180 pKa = 4.11SLEE183 pKa = 4.25TKK185 pKa = 10.44LALSKK190 pKa = 11.48DD191 pKa = 3.64EE192 pKa = 4.88FFPASIYY199 pKa = 10.49KK200 pKa = 10.1KK201 pKa = 9.22PNAMKK206 pKa = 10.22LQKK209 pKa = 10.35EE210 pKa = 4.36CLIKK214 pKa = 10.77AKK216 pKa = 10.48AADD219 pKa = 3.83PVLSAVAVPTNEE231 pKa = 4.3QISDD235 pKa = 3.37ALKK238 pKa = 9.49QFRR241 pKa = 11.84EE242 pKa = 4.06EE243 pKa = 4.09VEE245 pKa = 4.09RR246 pKa = 11.84QDD248 pKa = 3.3MVTFLSQQDD257 pKa = 3.3RR258 pKa = 11.84LHH260 pKa = 6.95RR261 pKa = 11.84LRR263 pKa = 11.84EE264 pKa = 4.01YY265 pKa = 10.5VGKK268 pKa = 10.59KK269 pKa = 9.19ILSFEE274 pKa = 4.18LVEE277 pKa = 4.25SGRR280 pKa = 11.84YY281 pKa = 9.11PIDD284 pKa = 4.99LSTSWLPWTASLLMKK299 pKa = 10.55YY300 pKa = 9.47PLSEE304 pKa = 3.98RR305 pKa = 11.84VSATIMIPMGTITFPSMSLKK325 pKa = 9.88IRR327 pKa = 11.84RR328 pKa = 11.84NLLEE332 pKa = 3.97VLKK335 pKa = 10.83VPGMNRR341 pKa = 11.84RR342 pKa = 11.84KK343 pKa = 9.52ILPSSAPSRR352 pKa = 11.84LGASVPRR359 pKa = 11.84AQGVAPLLQGGARR372 pKa = 11.84LRR374 pKa = 11.84VLRR377 pKa = 11.84APVAPDD383 pKa = 3.09TRR385 pKa = 11.84GIPFARR391 pKa = 11.84SKK393 pKa = 10.47WEE395 pKa = 3.77AGVRR399 pKa = 11.84KK400 pKa = 9.81IIGGGEE406 pKa = 4.02MNDD409 pKa = 3.16WLQVSSKK416 pKa = 9.55EE417 pKa = 4.05RR418 pKa = 11.84CGGNASDD425 pKa = 4.73ALLMLADD432 pKa = 4.39ASDD435 pKa = 3.91RR436 pKa = 11.84LPGRR440 pKa = 11.84ILRR443 pKa = 11.84GLFTLRR449 pKa = 11.84GARR452 pKa = 11.84EE453 pKa = 4.05ALRR456 pKa = 11.84LPSGLSVPDD465 pKa = 4.16GRR467 pKa = 11.84ACCNVKK473 pKa = 10.16HH474 pKa = 6.17FNVEE478 pKa = 3.59ATAGPFLRR486 pKa = 11.84AFGVKK491 pKa = 9.73KK492 pKa = 10.23KK493 pKa = 10.55AALEE497 pKa = 3.83QLLGDD502 pKa = 4.21FVWDD506 pKa = 4.22CFDD509 pKa = 5.64DD510 pKa = 4.26FASNDD515 pKa = 2.82GDD517 pKa = 3.65ARR519 pKa = 11.84RR520 pKa = 11.84LPFFGMRR527 pKa = 11.84IGFRR531 pKa = 11.84TKK533 pKa = 10.43LLPRR537 pKa = 11.84SEE539 pKa = 4.5MLTKK543 pKa = 10.43IKK545 pKa = 10.42DD546 pKa = 3.82FKK548 pKa = 11.0PLGRR552 pKa = 11.84CVMMLDD558 pKa = 5.12AIEE561 pKa = 4.41QFCSSPLYY569 pKa = 10.69NILSNLSADD578 pKa = 3.72ALKK581 pKa = 10.97DD582 pKa = 3.47PLSGFRR588 pKa = 11.84NTAVRR593 pKa = 11.84ASSDD597 pKa = 2.64WSYY600 pKa = 9.94MWEE603 pKa = 4.11EE604 pKa = 3.63IKK606 pKa = 10.32QAKK609 pKa = 9.22VCMEE613 pKa = 4.52LDD615 pKa = 2.81WSKK618 pKa = 10.93FDD620 pKa = 4.34RR621 pKa = 11.84EE622 pKa = 4.12RR623 pKa = 11.84PRR625 pKa = 11.84DD626 pKa = 3.75DD627 pKa = 5.03LEE629 pKa = 4.93FIVDD633 pKa = 3.9LVISCFTPRR642 pKa = 11.84TEE644 pKa = 4.2RR645 pKa = 11.84EE646 pKa = 3.69QRR648 pKa = 11.84LLRR651 pKa = 11.84AYY653 pKa = 7.36EE654 pKa = 3.78ICMKK658 pKa = 10.24RR659 pKa = 11.84AIVEE663 pKa = 4.07RR664 pKa = 11.84VAILDD669 pKa = 4.0DD670 pKa = 4.26GALFTIDD677 pKa = 3.62GMVPSGSLWTGWIDD691 pKa = 3.2TALNILYY698 pKa = 10.47LSAALNQAGFSLDD711 pKa = 3.34TARR714 pKa = 11.84PKK716 pKa = 10.84CAGDD720 pKa = 3.88DD721 pKa = 3.71NLTLFLEE728 pKa = 4.76DD729 pKa = 3.4VTDD732 pKa = 3.37SRR734 pKa = 11.84LFYY737 pKa = 11.23VRR739 pKa = 11.84DD740 pKa = 3.66LLNEE744 pKa = 3.88WFLAGIKK751 pKa = 10.28EE752 pKa = 3.93KK753 pKa = 10.85DD754 pKa = 3.71FNITRR759 pKa = 11.84PPFYY763 pKa = 10.83VEE765 pKa = 4.55TYY767 pKa = 10.08QAVFPPGTDD776 pKa = 3.27LSLGTSKK783 pKa = 10.49IVDD786 pKa = 3.19QCEE789 pKa = 3.71WVRR792 pKa = 11.84FEE794 pKa = 3.86GQLYY798 pKa = 8.99INQEE802 pKa = 4.19EE803 pKa = 4.79GHH805 pKa = 4.64SHH807 pKa = 5.64RR808 pKa = 11.84WQYY811 pKa = 10.64RR812 pKa = 11.84FKK814 pKa = 10.9GKK816 pKa = 9.57PKK818 pKa = 10.28FLSCYY823 pKa = 7.99WLQDD827 pKa = 3.25GRR829 pKa = 11.84PIRR832 pKa = 11.84PASDD836 pKa = 3.2NVEE839 pKa = 3.77KK840 pKa = 10.96LLFPEE845 pKa = 6.28GIHH848 pKa = 7.15KK849 pKa = 10.8DD850 pKa = 2.93IDD852 pKa = 3.86DD853 pKa = 4.57YY854 pKa = 11.49IAAVLAMVVDD864 pKa = 4.12NPFNQHH870 pKa = 5.29NVNHH874 pKa = 6.16MKK876 pKa = 10.09HH877 pKa = 6.55RR878 pKa = 11.84YY879 pKa = 9.24LIAQQIKK886 pKa = 8.71RR887 pKa = 11.84VMVSTIPYY895 pKa = 9.7QLILALSRR903 pKa = 11.84IRR905 pKa = 11.84SEE907 pKa = 4.04AGEE910 pKa = 4.96DD911 pKa = 3.27IPFPMIAPWRR921 pKa = 11.84RR922 pKa = 11.84FKK924 pKa = 10.98EE925 pKa = 4.07WIDD928 pKa = 3.41LDD930 pKa = 3.85KK931 pKa = 11.31YY932 pKa = 10.95PPVQEE937 pKa = 4.62WIRR940 pKa = 11.84DD941 pKa = 3.4FDD943 pKa = 4.41DD944 pKa = 4.63FVAGISGLYY953 pKa = 9.33VRR955 pKa = 11.84STTGGIDD962 pKa = 2.88AYY964 pKa = 10.26KK965 pKa = 10.72FMDD968 pKa = 5.37FIRR971 pKa = 11.84GDD973 pKa = 3.74SVIGEE978 pKa = 4.37GQWGNEE984 pKa = 3.58MTRR987 pKa = 11.84WIRR990 pKa = 11.84FVTEE994 pKa = 4.24HH995 pKa = 6.15PVSRR999 pKa = 11.84ALRR1002 pKa = 11.84KK1003 pKa = 9.42ARR1005 pKa = 11.84RR1006 pKa = 11.84FNPKK1010 pKa = 9.44AAPTAGEE1017 pKa = 3.82NLFYY1021 pKa = 10.87TRR1023 pKa = 11.84AQGGLNAYY1031 pKa = 9.38RR1032 pKa = 11.84RR1033 pKa = 11.84QLQNHH1038 pKa = 5.79RR1039 pKa = 11.84LEE1041 pKa = 4.33SSEE1044 pKa = 5.37EE1045 pKa = 3.77YY1046 pKa = 10.85GLWVSDD1052 pKa = 4.98LLRR1055 pKa = 11.84QKK1057 pKa = 10.96

Molecular weight:
119.83 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1439

382

1057

719.5

80.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.771 ± 1.778

1.042 ± 0.254

6.81 ± 0.383

6.463 ± 0.682

5.49 ± 0.125

6.254 ± 0.142

1.112 ± 0.032

5.073 ± 0.434

5.629 ± 0.192

9.521 ± 0.946

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.085 ± 0.38

2.849 ± 0.37

4.656 ± 0.486

3.683 ± 0.376

8.061 ± 0.102

5.698 ± 0.227

4.795 ± 0.473

5.629 ± 0.065

1.529 ± 0.621

2.849 ± 0.015

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski