Oscillibacter sp. 1-3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Oscillibacter; unclassified Oscillibacter

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4209 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9M9E2|R9M9E2_9FIRM N-acetyltransferase domain-containing protein OS=Oscillibacter sp. 1-3 OX=1235797 GN=C816_00196 PE=4 SV=1
MM1 pKa = 7.06MKK3 pKa = 10.34KK4 pKa = 10.48FFGILLALAMVLSLAACGGNGGSGTPANDD33 pKa = 3.6TPPASTDD40 pKa = 3.13NNQPSGDD47 pKa = 3.87APSGGSASGTFKK59 pKa = 11.07LGGVGPLTGDD69 pKa = 3.21AAIYY73 pKa = 10.57GNAAMNGAQIAVDD86 pKa = 4.64EE87 pKa = 4.45INALGGIQIEE97 pKa = 4.99FKK99 pKa = 11.22AEE101 pKa = 3.76DD102 pKa = 3.86DD103 pKa = 4.06VADD106 pKa = 4.51GEE108 pKa = 4.82TSVNAYY114 pKa = 6.94NTLMDD119 pKa = 3.01WGMQVLVGPVTTGAAIAVSAEE140 pKa = 4.09VYY142 pKa = 10.06NDD144 pKa = 3.21RR145 pKa = 11.84VFALTPSASSTDD157 pKa = 3.78VISGKK162 pKa = 10.72DD163 pKa = 3.3NMFQVCFTDD172 pKa = 3.87PNQGVGSADD181 pKa = 3.72YY182 pKa = 10.36ISQNMPGAKK191 pKa = 9.8VGIIYY196 pKa = 10.56RR197 pKa = 11.84NDD199 pKa = 3.26DD200 pKa = 3.86AYY202 pKa = 11.56SQGIRR207 pKa = 11.84DD208 pKa = 3.89TFVAEE213 pKa = 4.0AGKK216 pKa = 10.65VGLEE220 pKa = 3.96IVSEE224 pKa = 4.51GTFTKK229 pKa = 9.81DD230 pKa = 2.99TQSDD234 pKa = 3.74FSVQLTAAQSSGADD248 pKa = 3.82LIFLPTYY255 pKa = 7.17YY256 pKa = 10.3QPNAVILNQAKK267 pKa = 10.12GMGYY271 pKa = 10.26APTFFGVDD279 pKa = 3.25GMDD282 pKa = 5.46GILAMPGFDD291 pKa = 3.04TSLAEE296 pKa = 4.23GVMLLTPFSADD307 pKa = 3.16ADD309 pKa = 4.02DD310 pKa = 4.74QRR312 pKa = 11.84TQSFVAKK319 pKa = 10.04YY320 pKa = 10.38NEE322 pKa = 4.18LHH324 pKa = 6.85GEE326 pKa = 4.22TPNQFAADD334 pKa = 4.42GYY336 pKa = 9.1DD337 pKa = 2.97AVYY340 pKa = 10.19IIYY343 pKa = 9.42EE344 pKa = 4.11ALQAAGCSGDD354 pKa = 3.32MSAEE358 pKa = 4.28EE359 pKa = 3.74ICEE362 pKa = 4.34AIVAVMPTITVDD374 pKa = 3.18GLTGQGMTWAEE385 pKa = 3.94SGEE388 pKa = 4.24VSKK391 pKa = 11.52APMAVVIKK399 pKa = 10.44DD400 pKa = 3.6GVYY403 pKa = 8.55VTPP406 pKa = 4.86

Molecular weight: 41.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9M200|R9M200_9FIRM Uncharacterized protein OS=Oscillibacter sp. 1-3 OX=1235797 GN=C816_02573 PE=4 SV=1
MM1 pKa = 7.34PRR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84IQLNAVQTLALGFAALIVAGGVLLALPVSSRR36 pKa = 11.84DD37 pKa = 3.26GHH39 pKa = 6.61ALPFLDD45 pKa = 5.03ALFTAASASCVTGLVLYY62 pKa = 7.43DD63 pKa = 3.32TCTQFTLFGQAVILLLIQVGGMGFMTVSILVAVLLHH99 pKa = 6.03RR100 pKa = 11.84RR101 pKa = 11.84IGLRR105 pKa = 11.84QRR107 pKa = 11.84SILMDD112 pKa = 3.58SVGALQMGGIVRR124 pKa = 11.84LTRR127 pKa = 11.84RR128 pKa = 11.84AIRR131 pKa = 11.84VTLTCEE137 pKa = 3.84GAGAALLALWFCPRR151 pKa = 11.84YY152 pKa = 9.92GVGRR156 pKa = 11.84GLWMSLFHH164 pKa = 6.87AVSSFCNAGFDD175 pKa = 4.52LLGTGASLTSIAGEE189 pKa = 3.83PLPNIVLMALVICGGLGFLVWDD211 pKa = 4.72DD212 pKa = 4.28LLTHH216 pKa = 6.55GRR218 pKa = 11.84HH219 pKa = 4.29IRR221 pKa = 11.84RR222 pKa = 11.84WRR224 pKa = 11.84LHH226 pKa = 5.15SKK228 pKa = 9.71IVVFATTLLFVGGAAAFYY246 pKa = 10.4FLEE249 pKa = 5.07RR250 pKa = 11.84DD251 pKa = 3.37HH252 pKa = 7.41AFAGAGPGRR261 pKa = 11.84RR262 pKa = 11.84ALMAAFQSVTCRR274 pKa = 11.84TAGFNTAPLTALSQSGTLLTMILMFIGAGSGSTGGGAKK312 pKa = 10.31VNTVSVLLLSALAQVRR328 pKa = 11.84RR329 pKa = 11.84KK330 pKa = 10.17EE331 pKa = 3.97DD332 pKa = 3.12VNVFRR337 pKa = 11.84RR338 pKa = 11.84RR339 pKa = 11.84LDD341 pKa = 3.05TATIQKK347 pKa = 9.93AYY349 pKa = 10.16SSVSIFFLACLAGTMVMCLQGISLDD374 pKa = 3.7SALFEE379 pKa = 5.36AISAVGTVGLTRR391 pKa = 11.84GVTPYY396 pKa = 10.81LPEE399 pKa = 4.31LSKK402 pKa = 10.69LTVLLMMFAGRR413 pKa = 11.84VGSMSVAMAVTRR425 pKa = 11.84DD426 pKa = 3.36RR427 pKa = 11.84PQPKK431 pKa = 9.68LRR433 pKa = 11.84NIPEE437 pKa = 4.73KK438 pKa = 10.7ILIGG442 pKa = 3.81

Molecular weight: 46.92 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4209

0

4209

1246555

25

2967

296.2

32.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.764 ± 0.05

1.675 ± 0.019

5.457 ± 0.03

7.055 ± 0.038

3.762 ± 0.029

7.914 ± 0.039

1.738 ± 0.018

5.497 ± 0.036

4.563 ± 0.038

9.875 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.704 ± 0.02

3.285 ± 0.024

4.303 ± 0.031

3.511 ± 0.026

6.192 ± 0.048

5.577 ± 0.025

5.61 ± 0.05

6.86 ± 0.035

1.138 ± 0.016

3.518 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski