Amino acid dipepetide frequency for Bifidobacterium biavatii DSM 23969

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.212AlaAla: 15.212 ± 0.199	1.116AlaCys: 1.116 ± 0.038	8.587AlaAsp: 8.587 ± 0.13	6.081AlaGlu: 6.081 ± 0.091	3.599AlaPhe: 3.599 ± 0.07	9.695AlaGly: 9.695 ± 0.123	2.359AlaHis: 2.359 ± 0.058	5.568AlaIle: 5.568 ± 0.085	4.756AlaLys: 4.756 ± 0.106	9.693AlaLeu: 9.693 ± 0.132	3.118AlaMet: 3.118 ± 0.064	3.783AlaAsn: 3.783 ± 0.076	4.05AlaPro: 4.05 ± 0.074	3.92AlaGln: 3.92 ± 0.08	6.364AlaArg: 6.364 ± 0.094	6.69AlaSer: 6.69 ± 0.086	6.765AlaThr: 6.765 ± 0.095	8.639AlaVal: 8.639 ± 0.113	1.647AlaTrp: 1.647 ± 0.046	2.855AlaTyr: 2.855 ± 0.053	0.001AlaXaa: 0.001 ± 0.001
Cys	1.179CysAla: 1.179 ± 0.036	0.134CysCys: 0.134 ± 0.013	0.658CysAsp: 0.658 ± 0.033	0.52CysGlu: 0.52 ± 0.027	0.319CysPhe: 0.319 ± 0.02	1.004CysGly: 1.004 ± 0.038	0.196CysHis: 0.196 ± 0.014	0.393CysIle: 0.393 ± 0.021	0.251CysLys: 0.251 ± 0.016	0.779CysLeu: 0.779 ± 0.033	0.262CysMet: 0.262 ± 0.019	0.243CysAsn: 0.243 ± 0.018	0.452CysPro: 0.452 ± 0.02	0.202CysGln: 0.202 ± 0.017	0.615CysArg: 0.615 ± 0.028	0.535CysSer: 0.535 ± 0.025	0.55CysThr: 0.55 ± 0.023	0.743CysVal: 0.743 ± 0.029	0.127CysTrp: 0.127 ± 0.012	0.234CysTyr: 0.234 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	8.375AspAla: 8.375 ± 0.12	0.629AspCys: 0.629 ± 0.028	5.601AspAsp: 5.601 ± 0.11	4.809AspGlu: 4.809 ± 0.089	2.18AspPhe: 2.18 ± 0.063	6.852AspGly: 6.852 ± 0.108	1.478AspHis: 1.478 ± 0.046	3.127AspIle: 3.127 ± 0.063	2.555AspLys: 2.555 ± 0.064	5.304AspLeu: 5.304 ± 0.087	1.705AspMet: 1.705 ± 0.043	1.964AspAsn: 1.964 ± 0.054	3.851AspPro: 3.851 ± 0.07	1.983AspGln: 1.983 ± 0.056	4.005AspArg: 4.005 ± 0.062	3.472AspSer: 3.472 ± 0.068	3.76AspThr: 3.76 ± 0.077	5.346AspVal: 5.346 ± 0.08	1.152AspTrp: 1.152 ± 0.039	2.107AspTyr: 2.107 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.609GluAla: 5.609 ± 0.1	0.454GluCys: 0.454 ± 0.025	2.912GluAsp: 2.912 ± 0.063	2.733GluGlu: 2.733 ± 0.072	1.718GluPhe: 1.718 ± 0.048	3.36GluGly: 3.36 ± 0.063	1.671GluHis: 1.671 ± 0.043	2.629GluIle: 2.629 ± 0.057	1.881GluLys: 1.881 ± 0.061	5.031GluLeu: 5.031 ± 0.088	1.238GluMet: 1.238 ± 0.039	1.717GluAsn: 1.717 ± 0.043	2.782GluPro: 2.782 ± 0.062	2.5GluGln: 2.5 ± 0.055	4.551GluArg: 4.551 ± 0.087	3.115GluSer: 3.115 ± 0.059	3.498GluThr: 3.498 ± 0.065	2.904GluVal: 2.904 ± 0.067	0.7GluTrp: 0.7 ± 0.03	1.778GluTyr: 1.778 ± 0.05	0.001GluXaa: 0.001 ± 0.001
Phe	4.08PheAla: 4.08 ± 0.083	0.339PheCys: 0.339 ± 0.02	2.785PheAsp: 2.785 ± 0.062	1.659PheGlu: 1.659 ± 0.04	1.134PhePhe: 1.134 ± 0.042	3.197PheGly: 3.197 ± 0.076	0.662PheHis: 0.662 ± 0.026	1.484PheIle: 1.484 ± 0.039	1.141PheLys: 1.141 ± 0.041	2.604PheLeu: 2.604 ± 0.057	0.816PheMet: 0.816 ± 0.034	1.183PheAsn: 1.183 ± 0.035	1.314PhePro: 1.314 ± 0.039	0.869PheGln: 0.869 ± 0.032	1.826PheArg: 1.826 ± 0.042	2.078PheSer: 2.078 ± 0.052	2.283PheThr: 2.283 ± 0.058	2.628PheVal: 2.628 ± 0.056	0.425PheTrp: 0.425 ± 0.024	0.916PheTyr: 0.916 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	7.533GlyAla: 7.533 ± 0.119	0.856GlyCys: 0.856 ± 0.037	5.325GlyAsp: 5.325 ± 0.098	4.579GlyGlu: 4.579 ± 0.076	3.059GlyPhe: 3.059 ± 0.075	6.35GlyGly: 6.35 ± 0.111	1.741GlyHis: 1.741 ± 0.04	4.43GlyIle: 4.43 ± 0.074	3.914GlyLys: 3.914 ± 0.082	6.971GlyLeu: 6.971 ± 0.103	2.267GlyMet: 2.267 ± 0.047	2.961GlyAsn: 2.961 ± 0.079	2.473GlyPro: 2.473 ± 0.054	2.487GlyGln: 2.487 ± 0.066	4.898GlyArg: 4.898 ± 0.083	5.257GlySer: 5.257 ± 0.083	5.588GlyThr: 5.588 ± 0.11	6.346GlyVal: 6.346 ± 0.103	1.437GlyTrp: 1.437 ± 0.045	2.71GlyTyr: 2.71 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	2.413HisAla: 2.413 ± 0.057	0.22HisCys: 0.22 ± 0.015	1.764HisAsp: 1.764 ± 0.045	1.204HisGlu: 1.204 ± 0.037	0.583HisPhe: 0.583 ± 0.027	1.994HisGly: 1.994 ± 0.047	0.553HisHis: 0.553 ± 0.025	1.051HisIle: 1.051 ± 0.037	0.527HisLys: 0.527 ± 0.025	1.489HisLeu: 1.489 ± 0.039	0.554HisMet: 0.554 ± 0.026	0.585HisAsn: 0.585 ± 0.028	1.212HisPro: 1.212 ± 0.038	0.562HisGln: 0.562 ± 0.025	1.412HisArg: 1.412 ± 0.044	0.96HisSer: 0.96 ± 0.035	1.147HisThr: 1.147 ± 0.029	1.793HisVal: 1.793 ± 0.04	0.346HisTrp: 0.346 ± 0.021	0.68HisTyr: 0.68 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.66IleAla: 6.66 ± 0.1	0.552IleCys: 0.552 ± 0.023	4.074IleAsp: 4.074 ± 0.058	2.835IleGlu: 2.835 ± 0.066	1.331IlePhe: 1.331 ± 0.051	4.597IleGly: 4.597 ± 0.081	0.825IleHis: 0.825 ± 0.03	2.626IleIle: 2.626 ± 0.056	1.685IleLys: 1.685 ± 0.053	3.628IleLeu: 3.628 ± 0.072	1.164IleMet: 1.164 ± 0.037	1.72IleAsn: 1.72 ± 0.046	2.585IlePro: 2.585 ± 0.054	1.216IleGln: 1.216 ± 0.036	2.942IleArg: 2.942 ± 0.06	2.715IleSer: 2.715 ± 0.057	3.311IleThr: 3.311 ± 0.07	4.754IleVal: 4.754 ± 0.069	0.568IleTrp: 0.568 ± 0.025	1.118IleTyr: 1.118 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	4.553LysAla: 4.553 ± 0.098	0.16LysCys: 0.16 ± 0.015	2.403LysAsp: 2.403 ± 0.063	1.894LysGlu: 1.894 ± 0.058	1.004LysPhe: 1.004 ± 0.041	2.504LysGly: 2.504 ± 0.066	0.772LysHis: 0.772 ± 0.031	1.738LysIle: 1.738 ± 0.059	1.641LysLys: 1.641 ± 0.057	3.172LysLeu: 3.172 ± 0.065	0.834LysMet: 0.834 ± 0.03	1.374LysAsn: 1.374 ± 0.045	2.392LysPro: 2.392 ± 0.057	1.451LysGln: 1.451 ± 0.045	2.371LysArg: 2.371 ± 0.057	2.146LysSer: 2.146 ± 0.05	2.882LysThr: 2.882 ± 0.067	2.525LysVal: 2.525 ± 0.069	0.485LysTrp: 0.485 ± 0.026	1.095LysTyr: 1.095 ± 0.037	0.0LysXaa: 0.0 ± 0.0
Leu	10.336LeuAla: 10.336 ± 0.116	0.909LeuCys: 0.909 ± 0.033	6.083LeuAsp: 6.083 ± 0.093	4.048LeuGlu: 4.048 ± 0.066	3.025LeuPhe: 3.025 ± 0.071	6.654LeuGly: 6.654 ± 0.085	1.767LeuHis: 1.767 ± 0.046	4.475LeuIle: 4.475 ± 0.094	3.244LeuLys: 3.244 ± 0.068	7.654LeuLeu: 7.654 ± 0.133	2.109LeuMet: 2.109 ± 0.057	2.943LeuAsn: 2.943 ± 0.053	4.527LeuPro: 4.527 ± 0.071	2.195LeuGln: 2.195 ± 0.049	5.285LeuArg: 5.285 ± 0.095	5.265LeuSer: 5.265 ± 0.087	5.634LeuThr: 5.634 ± 0.081	6.261LeuVal: 6.261 ± 0.094	1.093LeuTrp: 1.093 ± 0.035	2.131LeuTyr: 2.131 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.591MetAla: 2.591 ± 0.049	0.253MetCys: 0.253 ± 0.018	1.376MetAsp: 1.376 ± 0.043	0.982MetGlu: 0.982 ± 0.032	0.875MetPhe: 0.875 ± 0.033	1.665MetGly: 1.665 ± 0.046	0.51MetHis: 0.51 ± 0.024	1.481MetIle: 1.481 ± 0.041	0.86MetLys: 0.86 ± 0.03	2.593MetLeu: 2.593 ± 0.054	0.703MetMet: 0.703 ± 0.03	1.011MetAsn: 1.011 ± 0.031	1.484MetPro: 1.484 ± 0.043	0.811MetGln: 0.811 ± 0.035	1.801MetArg: 1.801 ± 0.048	1.804MetSer: 1.804 ± 0.046	2.043MetThr: 2.043 ± 0.053	1.634MetVal: 1.634 ± 0.047	0.322MetTrp: 0.322 ± 0.019	0.524MetTyr: 0.524 ± 0.024	0.001MetXaa: 0.001 ± 0.001
Asn	4.025AsnAla: 4.025 ± 0.077	0.21AsnCys: 0.21 ± 0.017	2.431AsnAsp: 2.431 ± 0.055	1.726AsnGlu: 1.726 ± 0.044	0.834AsnPhe: 0.834 ± 0.032	3.766AsnGly: 3.766 ± 0.089	0.671AsnHis: 0.671 ± 0.028	1.532AsnIle: 1.532 ± 0.044	1.198AsnLys: 1.198 ± 0.042	2.594AsnLeu: 2.594 ± 0.055	0.814AsnMet: 0.814 ± 0.031	1.229AsnAsn: 1.229 ± 0.051	2.183AsnPro: 2.183 ± 0.05	0.96AsnGln: 0.96 ± 0.039	1.991AsnArg: 1.991 ± 0.043	1.682AsnSer: 1.682 ± 0.053	2.128AsnThr: 2.128 ± 0.059	2.579AsnVal: 2.579 ± 0.056	0.469AsnTrp: 0.469 ± 0.026	0.905AsnTyr: 0.905 ± 0.038	0.0AsnXaa: 0.0 ± 0.0
Pro	5.53ProAla: 5.53 ± 0.096	0.344ProCys: 0.344 ± 0.019	3.843ProAsp: 3.843 ± 0.068	2.925ProGlu: 2.925 ± 0.059	1.689ProPhe: 1.689 ± 0.048	3.483ProGly: 3.483 ± 0.066	0.947ProHis: 0.947 ± 0.035	2.29ProIle: 2.29 ± 0.047	1.676ProLys: 1.676 ± 0.048	3.77ProLeu: 3.77 ± 0.073	1.08ProMet: 1.08 ± 0.038	1.674ProAsn: 1.674 ± 0.042	1.328ProPro: 1.328 ± 0.046	1.701ProGln: 1.701 ± 0.045	2.302ProArg: 2.302 ± 0.056	3.103ProSer: 3.103 ± 0.064	2.97ProThr: 2.97 ± 0.058	3.974ProVal: 3.974 ± 0.064	0.695ProTrp: 0.695 ± 0.029	1.447ProTyr: 1.447 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	3.475GlnAla: 3.475 ± 0.084	0.3GlnCys: 0.3 ± 0.017	1.575GlnAsp: 1.575 ± 0.042	1.364GlnGlu: 1.364 ± 0.047	1.036GlnPhe: 1.036 ± 0.033	2.109GlnGly: 2.109 ± 0.049	0.746GlnHis: 0.746 ± 0.025	1.778GlnIle: 1.778 ± 0.044	0.959GlnLys: 0.959 ± 0.038	3.217GlnLeu: 3.217 ± 0.059	0.901GlnMet: 0.901 ± 0.034	0.968GlnAsn: 0.968 ± 0.035	1.763GlnPro: 1.763 ± 0.06	1.528GlnGln: 1.528 ± 0.053	2.175GlnArg: 2.175 ± 0.057	2.136GlnSer: 2.136 ± 0.05	1.98GlnThr: 1.98 ± 0.052	2.253GlnVal: 2.253 ± 0.06	0.612GlnTrp: 0.612 ± 0.028	1.127GlnTyr: 1.127 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	5.741ArgAla: 5.741 ± 0.089	0.538ArgCys: 0.538 ± 0.024	4.061ArgAsp: 4.061 ± 0.077	3.787ArgGlu: 3.787 ± 0.067	2.415ArgPhe: 2.415 ± 0.053	3.959ArgGly: 3.959 ± 0.072	1.562ArgHis: 1.562 ± 0.046	3.625ArgIle: 3.625 ± 0.065	2.332ArgLys: 2.332 ± 0.055	5.756ArgLeu: 5.756 ± 0.101	1.943ArgMet: 1.943 ± 0.046	2.105ArgAsn: 2.105 ± 0.056	2.711ArgPro: 2.711 ± 0.063	2.219ArgGln: 2.219 ± 0.055	5.219ArgArg: 5.219 ± 0.12	3.279ArgSer: 3.279 ± 0.067	3.565ArgThr: 3.565 ± 0.059	4.337ArgVal: 4.337 ± 0.079	0.957ArgTrp: 0.957 ± 0.035	2.068ArgTyr: 2.068 ± 0.05	0.002ArgXaa: 0.002 ± 0.001
Ser	6.578SerAla: 6.578 ± 0.089	0.539SerCys: 0.539 ± 0.023	4.157SerAsp: 4.157 ± 0.084	2.795SerGlu: 2.795 ± 0.054	2.064SerPhe: 2.064 ± 0.051	5.721SerGly: 5.721 ± 0.087	1.11SerHis: 1.11 ± 0.033	2.874SerIle: 2.874 ± 0.055	2.109SerLys: 2.109 ± 0.061	5.216SerLeu: 5.216 ± 0.074	1.466SerMet: 1.466 ± 0.044	2.002SerAsn: 2.002 ± 0.054	2.495SerPro: 2.495 ± 0.051	1.938SerGln: 1.938 ± 0.048	3.458SerArg: 3.458 ± 0.078	4.25SerSer: 4.25 ± 0.117	3.732SerThr: 3.732 ± 0.07	4.549SerVal: 4.549 ± 0.081	0.88SerTrp: 0.88 ± 0.03	1.666SerTyr: 1.666 ± 0.054	0.0SerXaa: 0.0 ± 0.0
Thr	7.326ThrAla: 7.326 ± 0.115	0.481ThrCys: 0.481 ± 0.022	4.389ThrAsp: 4.389 ± 0.084	2.693ThrGlu: 2.693 ± 0.051	2.241ThrPhe: 2.241 ± 0.053	5.559ThrGly: 5.559 ± 0.091	1.17ThrHis: 1.17 ± 0.035	3.65ThrIle: 3.65 ± 0.066	2.187ThrLys: 2.187 ± 0.052	5.794ThrLeu: 5.794 ± 0.085	1.466ThrMet: 1.466 ± 0.045	2.134ThrAsn: 2.134 ± 0.057	3.498ThrPro: 3.498 ± 0.064	1.815ThrGln: 1.815 ± 0.05	3.273ThrArg: 3.273 ± 0.061	3.625ThrSer: 3.625 ± 0.07	4.222ThrThr: 4.222 ± 0.092	6.142ThrVal: 6.142 ± 0.101	0.879ThrTrp: 0.879 ± 0.034	1.752ThrTyr: 1.752 ± 0.056	0.0ThrXaa: 0.0 ± 0.0
Val	8.866ValAla: 8.866 ± 0.133	0.866ValCys: 0.866 ± 0.033	5.233ValAsp: 5.233 ± 0.077	3.916ValGlu: 3.916 ± 0.072	2.775ValPhe: 2.775 ± 0.059	5.171ValGly: 5.171 ± 0.088	1.381ValHis: 1.381 ± 0.039	4.236ValIle: 4.236 ± 0.071	2.946ValLys: 2.946 ± 0.071	6.45ValLeu: 6.45 ± 0.11	1.851ValMet: 1.851 ± 0.051	2.706ValAsn: 2.706 ± 0.049	3.895ValPro: 3.895 ± 0.057	1.982ValGln: 1.982 ± 0.05	4.617ValArg: 4.617 ± 0.084	5.069ValSer: 5.069 ± 0.066	5.543ValThr: 5.543 ± 0.1	5.885ValVal: 5.885 ± 0.101	1.05ValTrp: 1.05 ± 0.033	1.918ValTyr: 1.918 ± 0.051	0.0ValXaa: 0.0 ± 0.0
Trp	1.241TrpAla: 1.241 ± 0.03	0.162TrpCys: 0.162 ± 0.013	0.927TrpAsp: 0.927 ± 0.04	0.587TrpGlu: 0.587 ± 0.024	0.574TrpPhe: 0.574 ± 0.028	0.932TrpGly: 0.932 ± 0.034	0.369TrpHis: 0.369 ± 0.02	0.772TrpIle: 0.772 ± 0.03	0.625TrpLys: 0.625 ± 0.026	1.497TrpLeu: 1.497 ± 0.047	0.398TrpMet: 0.398 ± 0.022	0.697TrpAsn: 0.697 ± 0.029	0.553TrpPro: 0.553 ± 0.026	0.67TrpGln: 0.67 ± 0.027	1.064TrpArg: 1.064 ± 0.037	0.903TrpSer: 0.903 ± 0.034	0.979TrpThr: 0.979 ± 0.038	0.885TrpVal: 0.885 ± 0.032	0.307TrpTrp: 0.307 ± 0.018	0.492TrpTyr: 0.492 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.173TyrAla: 3.173 ± 0.063	0.333TyrCys: 0.333 ± 0.019	2.07TyrAsp: 2.07 ± 0.053	1.646TyrGlu: 1.646 ± 0.042	0.97TyrPhe: 0.97 ± 0.035	2.609TyrGly: 2.609 ± 0.074	0.555TyrHis: 0.555 ± 0.024	1.147TyrIle: 1.147 ± 0.038	0.909TyrLys: 0.909 ± 0.037	2.427TyrLeu: 2.427 ± 0.05	0.636TyrMet: 0.636 ± 0.024	0.929TyrAsn: 0.929 ± 0.035	1.221TyrPro: 1.221 ± 0.038	0.995TyrGln: 0.995 ± 0.037	2.011TyrArg: 2.011 ± 0.048	1.517TyrSer: 1.517 ± 0.049	1.715TyrThr: 1.715 ± 0.056	2.178TyrVal: 2.178 ± 0.048	0.483TyrTrp: 0.483 ± 0.023	0.86TyrTyr: 0.86 ± 0.036	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.002XaaThr: 0.002 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.003

Statistics based on 2557 proteins (931382 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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